Markers of Oxidative Stress and Antioxidant Status in the Plasma, Urine and Saliva of Healthy Mice

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment. Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.

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Effect of percutaneous transthoracic lung biopsy on oxidative metabolism in sheep

Journal of the South African Veterinary Association ◽

10.4102/jsava.v83i1.14 ◽

2012 ◽

Vol 83 (1) ◽

Cited By ~ 2

Author(s):

Andreza A. Silva ◽

Danilo O.L. Ferreira ◽

Bianca P. Santarosa ◽

Adriano Dias ◽

Débora C. Damasceno ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Metabolism ◽

Lung Biopsy ◽

Thiobarbituric Acid ◽

Oxidative Stress Markers ◽

Thiobarbituric Acid Reactive Substances ◽

Total Glutathione ◽

Blood Samples ◽

Stress Markers ◽

Healthy Sheep

This study aimed to assess the effect of percutaneous transthoracic lung biopsy on the oxidative metabolism of sheep by measuring the oxidative stress markers of superoxide dismutase (SOD), total glutathione (GSH-t), peroxidase (GSH-Px) and thiobarbituric acid reactive substances (TBARS) in the red cells of these animals. Blood samples were collected from 20 clinically healthy sheep prior to, and 30 min after, percutaneous transthoracic lung biopsy. After biopsy, there was a significant decrease (p < 0.05) in SOD and GSH-Px activity, with no significant change (p ≥ 0.05) in GSH-t and TBARS concentrations. These results showed that percutaneous transthoracic lung biopsy did not significantly affect the oxidative metabolism of sheep 30 min after the procedure, which may be used widely in this species without causing serious tissue damage.

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Effect of different models of physical exercise on oxidative stress markers in mouse liver

Applied Physiology Nutrition and Metabolism ◽

10.1139/h08-132 ◽

2009 ◽

Vol 34 (1) ◽

pp. 60-65 ◽

Cited By ~ 21

Author(s):

Luciano A. da Silva ◽

Cleber A. Pinho ◽

Luis G.C. Rocha ◽

Talita Tuon ◽

Paulo C.L. Silveira ◽

...

Keyword(s):

Oxidative Stress ◽

Physical Exercise ◽

Mouse Liver ◽

Citrate Synthase ◽

Training Session ◽

Thiobarbituric Acid ◽

Oxidative Stress Markers ◽

Sod Activity ◽

Stress Markers ◽

Continuous Running

The aim of this study was to investigate the effect of different protocols of physical exercise on oxidative stress markers in mouse liver. Twenty-eight male CF1 mice (30–35 g) were distributed into 4 groups (n = 7) — untrained (UT), continuous running (CR), downhill running (D-HR), and intermittent running (IR) — and underwent an 8-week training program. Forty-eight hours after the last training session, the animals were killed, and their livers were removed. Blood lactate, creatine kinase, citrate synthase, thiobarbituric acid reactive species, carbonyl, superoxide dismutase (SOD), and catalase (CAT) activities were assayed. Results show a decrease in the level of lipoperoxidation and protein carbonylation in the CR and D-HR groups. SOD activity was significantly increased and CAT activity was reduced in the CR and D-HR groups. Our findings indicate that CR and D-HR may be important for decreasing oxidative damage and in the regulation of antioxidant enzymes (SOD and CAT) in the livers of trained mice.

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Circadian Rhythms of Oxidative Stress Markers and Melatonin Metabolite in Patients with Xeroderma Pigmentosum Group A

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/5741517 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 5

Author(s):

Rie Miyata ◽

Naoyuki Tanuma ◽

Hiroshi Sakuma ◽

Masaharu Hayashi

Keyword(s):

Oxidative Stress ◽

Circadian Rhythm ◽

Diurnal Variation ◽

Xeroderma Pigmentosum ◽

Enzyme Linked Immunosorbent Assay ◽

Oxidative Stress Markers ◽

Antioxidant Power ◽

Stress Markers ◽

Group A ◽

Xeroderma Pigmentosum Group A

Xeroderma pigmentosum group A (XPA) is a genetic disorder in DNA nucleotide excision repair (NER) with severe neurological disorders, in which oxidative stress and disturbed melatonin metabolism may be involved. Herein we confirmed the diurnal variation of melatonin metabolites, oxidative stress markers, and antioxidant power in urine of patients with XPA and age-matched controls, using enzyme-linked immunosorbent assay (ELISA). The peak of 6-sulfatoxymelatonin, a metabolite of melatonin, was seen at 6:00 in both the XPA patients and controls, though the peak value is lower, specifically in the younger age group of XPA patients. The older XPA patients demonstrated an increase in the urinary levels of 8-hydroxy-2′-deoxyguanosine and hexanoyl-lysine, a marker of oxidative DNA damage and lipid peroxidation, having a robust peak at 6:00 and 18:00, respectively. In addition, the urinary level of total antioxidant power was decreased in the older XPA patients. Recently, it is speculated that oxidative stress and antioxidant properties may have a diurnal variation, and the circadian rhythm is likely to influence the NER itself. We believe that the administration of melatonin has the possibility of ameliorating the augmented oxidative stress in neurodegeneration, especially in the older XPA patients, modulating the melatonin metabolism and the circadian rhythm.

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Levels of oxidative stress markers: correlation with hepatic function and worm burden patients with schistosomiasis

Acta Parasitologica ◽

10.2478/s11686-012-0026-5 ◽

2012 ◽

Vol 57 (2) ◽

Cited By ~ 7

Author(s):

G. Cunha ◽

V. Silva ◽

K. Bessa ◽

M. Bitencourt ◽

U. Macêdo ◽

...

Keyword(s):

Oxidative Stress ◽

Schistosoma Mansoni ◽

Worm Burden ◽

Reduced Glutathione ◽

Public Health Problem ◽

Thiobarbituric Acid ◽

Hepatic Function ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Parasitic Load

AbstractSchistosomiasis is caused by Schistosoma mansoni and is a public health problem in Brazil. The typical granulomatous lesion is associated with the increase in the oxidative damage by generation of free radicals. The aim of this work was to correlate some oxidative stress markers with the worm burden on carriers of schistosomiasis (n = 30) in the acute phase in comparison to healthy subjects (n = 30). The pro-oxidant parameter used was the colorimetric quantification of reactive substances to thiobarbituric acid, while the antioxidant markers used were blood content of reduced glutathione and determination of the activity of catalase. The worm burden was assessed by Kato-Katz method. The results pointed out that initially there was no difference in the catalase activity. However, there was a positive correlation between the increase in parasitic load and intensity of lipid peroxidation, and decrease in the content of reduced glutathione. Additionally, only the aspartate aminotransferase levels presented to be high, while there was a decrease in bilirubin level. Therefore, a possible association between the establishment of the oxidative stress in tissue and the parasitic load of Schistosoma mansoni is suggested.

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Impact of environmentally relevant concentration of benzophenone-3 on antioxidant enzymes, oxidative stress markers and morphology of gills in Danio rerio (Hamilton)

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2021.14.3.0043 ◽

2021 ◽

Vol 14 (3) ◽

pp. 189-196

Author(s):

S Velanganni ◽

P Sivakumar ◽

S Miltonprabu

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Danio Rerio ◽

Thiobarbituric Acid ◽

Respiratory Physiology ◽

Control Fish ◽

Histopathological Analysis ◽

Oxidative Stress Markers ◽

Relevant Concentration ◽

Stress Markers

The current investigation intended to evaluate the effect of Benzophenone-3 (BP-3) at the environmentally relevant concentration (44 μg/L) in the gills of Danio rerio through evaluating oxidative stress markers and histopathological analysis. The adult Zebra fish was exposed to BP-3 at environmentally relevant concentration for 45 days. During the experimental period of 15, 30 and 45 days, lipid peroxidation markers like thiobarbituric acid reactive substances (TBARS) and Hydrogen peroxide (H2O2), antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) and non-enzymatic glutathione (GSH) in the gill and histology of gill were analyzed. The activity of TBARS and H2O2 was found to be significantly higher meanwhile the activities of antioxidant enzymes viz., SOD, CAT, GPx and Glutathione (GSH) level were found to be significantly reduced in the gill of BP-3 treated fish for 30 and 45 days. Additionally, the morphology of gill also showed several abnormal changes in their morphology when compared to control. BP-3 exposure for 15 days elicited only mild alterations in the biochemical and histopathological variables when compared to 30 and 45 days exposure. Further, the values were also non-significant when compared to the control fish. These results demonstrated that the treatment of BP-3 at environmentally relevant concentration could prominently alter the respiratory physiology and metabolism of the gills of Danio rerio.

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The Activity of Oxidative Stress Markers in Acute Respiratory Distress Syndrome / Oksidativa Stresa Marķieru Aktivitate Akuta Respiratora Distresa Sindroma Gadījuma

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.2478/prolas-2014-0032 ◽

2014 ◽

Vol 68 (5-6) ◽

pp. 247-249

Author(s):

Marina Šarkele ◽

Agnese Ozoliņa ◽

Olegs Sabeļnikovs ◽

Andrejs Šķesters ◽

Alise Silova ◽

...

Keyword(s):

Oxidative Stress ◽

Acute Respiratory Distress Syndrome ◽

Respiratory Distress Syndrome ◽

Respiratory Distress ◽

Distress Syndrome ◽

Thiobarbituric Acid ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Acid Substance ◽

Different Levels

Abstract Acute respiratory distress syndrome is a common complication characterised by severe hypoxemia, which leads to high mortality rates in ICU patients. Imbalance between oxidative stress markers like oxidants and antioxidants may play an important role in pathophysiology of the syndrome. We observed 17 ARDS patients during seven days after inclusion, with the main goal to describe dynamic changes in the level of oxidative stress markers in patients with acute respiratory distress syndrome. We found that there are dynamic differences in the level of malondialdechyde (MDA) and nitric oxide (NO) in patients with acute respiratory distress syndrome. There were also different levels of oxidative stress markers in non-survivor compared with survivor groups. Increased level of an oxidant like a thiobarbituric acid substance with malondialdechyde (TBS_MDA) and antioxidant glutathionperoxidase (GPx) at the first day after inclusion was related with poor outcome in patients with acute respiratory distress syndrome.

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The Effect of Treadmill Treatment on Oxidative Stress Markers and Endogenous Antioxidant Status in Obesity Mice

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2018.397 ◽

2018 ◽

Vol 6 (10) ◽

pp. 1803-1808

Author(s):

Tiwuk Susantiningsih ◽

Roro Rukmi Windi Perdani ◽

Khairunnisa Berawi ◽

Sutopo Hadi

Keyword(s):

Oxidative Stress ◽

Antioxidant Status ◽

Specific Activity ◽

Oxidative Status ◽

Laboratory Research ◽

Oxidative Stress Markers ◽

Obese Mice ◽

Stress Markers ◽

Post Test ◽

Endogenous Antioxidant

AIM: This study aimed to determine the effect of treadmill treatment on oxidative stress markers and endogenous antioxidant status seen from MDA, GSH, MnSOD enzyme specific activity and blood catalase of obese mice. MATERIALS AND METHODS: This research is experimental laboratory research using post-test control design group only. The study lasted for 28 days and was divided into 4 groups of study, i.e., group K (normal control), KP (obesity control), P1 (obesity mice with 1 x 10-minute treadmill treatment a day), and P2 (obesity mice with 2 x 10-minute treadmill treatment a day). RESULTS: The treadmill treatment had an effect on the improvement of the oxidative status of mice with a decrease of MDA level of obesity mice blood (p ≤ 0.05) compared to KP control. An elevated endogenous antioxidant status of obesity mice was seen from elevated GSH levels, MnSOD specific activity and blood catalase of obesity mice (p ≤ 0.05) compared with KP controls. Treatment of 1 x 10-minute treadmill per day decreased blood MDA level, increased GSH enzyme and increased specific activity of MnSOD enzyme and blood catalase of obese mice. CONCLUSIONS: The 2 x 10-minute daily treadmill did not differ significantly in improving the oxidative status and endogenous antioxidant status compared with the treadmill 1 x 10 minutes a day (p ≥ 0.05).

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