scholarly journals Effect of percutaneous transthoracic lung biopsy on oxidative metabolism in sheep

2012 ◽  
Vol 83 (1) ◽  
Author(s):  
Andreza A. Silva ◽  
Danilo O.L. Ferreira ◽  
Bianca P. Santarosa ◽  
Adriano Dias ◽  
Débora C. Damasceno ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Metabolism ◽  
Lung Biopsy ◽  
Thiobarbituric Acid ◽  
Oxidative Stress Markers ◽  
Thiobarbituric Acid Reactive Substances ◽  
Total Glutathione ◽  
Blood Samples ◽  
Stress Markers ◽  
Healthy Sheep

This study aimed to assess the effect of percutaneous transthoracic lung biopsy on the oxidative metabolism of sheep by measuring the oxidative stress markers of superoxide dismutase (SOD), total glutathione (GSH-t), peroxidase (GSH-Px) and thiobarbituric acid reactive substances (TBARS) in the red cells of these animals. Blood samples were collected from 20 clinically healthy sheep prior to, and 30 min after, percutaneous transthoracic lung biopsy. After biopsy, there was a significant decrease (p < 0.05) in SOD and GSH-Px activity, with no significant change (p ≥ 0.05) in GSH-t and TBARS concentrations. These results showed that percutaneous transthoracic lung biopsy did not significantly affect the oxidative metabolism of sheep 30 min after the procedure, which may be used widely in this species without causing serious tissue damage.  

Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Shima Fathi ◽  
Mohammad Taghi Goodarzi ◽  
Shiva Borzouei ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Total Oxidative Stress ◽  
And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

scholarly journals Oxidative Stress, Telomere Shortening, and Apoptosis Associated to Sarcopenia and Frailty in Patients with Multimorbidity

2020 ◽  
Vol 9 (8) ◽  
pp. 2669 ◽  
Author(s):  
Máximo Bernabeu-Wittel ◽  
Raquel Gómez-Díaz ◽  
Álvaro González-Molina ◽  
Sofía Vidal-Serrano ◽  
Jesús Díez-Manglano ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Telomere Length ◽  
Prospective Observational Study ◽  
Oxygen Species ◽  
Oxidative Stress Markers ◽  
Blood Leukocytes ◽  
Telomere Shortening ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant

Background: The presence of oxidative stress, telomere shortening, and apoptosis in polypathological patients (PP) with sarcopenia and frailty remains unknown. Methods: Multicentric prospective observational study in order to assess oxidative stress markers (catalase, glutathione reductase (GR), total antioxidant capacity to reactive oxygen species (TAC-ROS), and superoxide dismutase (SOD)), absolute telomere length (aTL), and apoptosis (DNA fragmentation) in peripheral blood samples of a hospital-based population of PP. Associations of these biomarkers to sarcopenia, frailty, functional status, and 12-month mortality were analyzed. Results: Of the 444 recruited patients, 97 (21.8%), 278 (62.6%), and 80 (18%) were sarcopenic, frail, or both, respectively. Oxidative stress markers (lower TAC-ROS and higher SOD) were significantly enhanced and aTL significantly shortened in patients with sarcopenia, frailty or both syndromes. No evidence of apoptosis was detected in blood leukocytes of any of the patients. Both oxidative stress markers (GR, p = 0.04) and telomere shortening (p = 0.001) were associated to death risk and to less survival days. Conclusions: Oxidative stress markers and telomere length were enhanced and shortened, respectively, in blood samples of polypathological patients with sarcopenia and/or frailty. Both were associated to decreased survival. They could be useful in the clinical practice to assess vulnerable populations with multimorbidity and of potential interest as therapeutic targets.

scholarly journals PSI-36 Effect of short-chain fructooligosaccharides on Trolox equivalent antioxidant capacity and thiobarbituric acid reactive substances in mature and senior stock-type horses

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Emili McClure ◽  
Courtney P Heaton ◽  
Dishnu Sajeev ◽  
Thu Dinh
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Random Effect ◽  
Thiobarbituric Acid ◽  
Short Chain ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Thiobarbituric Acid Reactive Substances ◽  
Stress Markers ◽  
Trolox Equivalent ◽  
Stock Type

Abstract Oxidative stress (OS) causes health complications through the destruction of cellular components as individuals age. Reactive oxygen species are used to measure OS through Trolox equivalent antioxidant capacity (TEAC) and thiobarbituric acid reactive substances (TBARS). Other prebiotics have been used to reduce OS markers in numerous species; however, the effect of short-chain fructooligosaccharides (scFOS) on OS has not been studied in the horse. Ten healthy stock-type horses were blocked by age into 2 groups: mature (MA; n = 5; 7.0 ± 0.87 yr) and senior (SR; n = 5; 22.6 ± 1.1 yr) to analyze effects of scFOS on TEAC and TBARS. Horses were randomly assigned to 1 of 3 diets for 25 d before transition to another diet. Diets were bermudagrass hay offered at 1.5% BW/d hay as-fed, hay with a ration balancer (CON), or hay with a ration balancer and scFOS added at a rate of 2.5 g/kg (PRE). Prior to a total fecal collection for an alternate study, horses were fasted overnight for 8 h with blood samples taken immediately prior to feeding (0), 30, and 60 min postprandial. Oxidative stress markers were analyzed for the 2 ration balancer diets. Statistical analysis was performed with SAS using the MIXED procedure with horse within diet as a random effect with significance of P ≤ 0.05. Trolox equivalent antioxidant capacity was unaffected by diet (P = 0.827) or age (P = 0.347). Time (P = 0.006) was significant for TBARS which increased postprandial regardless of treatment or age. Consistent with other species, higher levels of OS was found in SR compared to MA regardless of time or diet (P = 0.037; 4.491 µM vs. 3.412 µM TBARS, respectively). These results indicate that scFOS do not seem to be effective in reducing OS in SR and MA horses.

scholarly journals Markers of Oxidative Stress and Antioxidant Status in the Plasma, Urine and Saliva of Healthy Mice

2018 ◽  
pp. 921-934 ◽  
Author(s):  
M. GYURÁSZOVÁ ◽  
A. KOVALČÍKOVÁ ◽  
K. JANŠÁKOVÁ ◽  
K. ŠEBEKOVÁ ◽  
P. CELEC ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Blood Collection ◽  
Reference Ranges ◽  
Oxidative Stress Markers ◽  
Male Adult ◽  
Antioxidant Power ◽  
Stress Markers ◽  
Status Assessment

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment. Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.

scholarly journals Influence of reproductive status on the daily rhythms of oxidative stress markers in Ovis aries

2010 ◽  
Vol 5 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Giuseppe Piccione ◽  
Claudia Giannetto ◽  
Francesco Fazio ◽  
Anna Assenza ◽  
Giovanni Caola
Keyword(s):  
Oxidative Stress ◽  
Circadian Rhythms ◽  
Ovis Aries ◽  
Reproductive Status ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Daily Rhythms ◽  
Blood Samples ◽  
Stress Markers ◽  
Experimental Group

AbstractChanges in circadian rhythms of dROMs, Oxy-ads and SHp during reproductive stages were studied in Comisana ewes. Twelve ewes were divided in two equal groups. The experimental group consisted of ewes undergoing gestation and lactation following artificial insemination and the control group consisted of non-pregnant ewes. Blood samples were collected every 3 h over a 24 h period, 20 days before insemination, on days 100 and 140 of pregnancy, on days 10, 30 and 200 post-partum and during the dry period. In the control group, blood samples were collected on the same days and with the same procedures as those used for the experimental group. A significant effect of time on all parameters studied was observed in the experimental group. Daily rhythms of the parameters studied were observed in the control group in all experimental conditions, and in the experimental group during pre-pregnancy and dry periods. We conclude that the reproductive status of sheep affects oxidative stress markers in blood and their circadian rhythms.

scholarly journals Association of advanced oxidation protein product, thiobarbituric acid reactive substances and total sulfhydryl groups with retinal blood vessels caliber

2019 ◽  
Vol 147 (11-12) ◽  
pp. 706-712
Author(s):  
Sonja Cekic ◽  
Tatjana Cvetkovic ◽  
Ivan Jovanovic ◽  
Predrag Jovanovic ◽  
Gordana Stankovic-Babic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Blood Vessels ◽  
Thiobarbituric Acid ◽  
Protein Product ◽  
Morphometric Parameters ◽  
Thiobarbituric Acid Reactive Substances ◽  
Advanced Oxidation Protein Product ◽  
Blood Samples ◽  
Retinal Blood Vessels

Introduction/Objective. Intensive oxidative stress is proven in patients with diabetes mellitus and important in the development of a microvascular complication of type 2 diabetes mellitus. The aim of the study was to investigate the relationship between morphometric parameters of retinal blood vessels in patients with diabetic retinopathy (DR) and the levels of parameters of oxidative stress: advanced oxidation protein product (AOPP), thiobarbituric acid reactive substances (TBARS), and total sulfhydryl (SH) groups in blood samples. Methods. The patients (the group with DR and controls) were sex- and age-matched. Glycaemia, hemoglobin A1C HbA1C, total cholesterol and its fractions, and triglycerides were measured in blood samples. AOPP and total SH groups were determined in the plasma by specific methods. Modification of the thiobarbituric acid method was used for the determination of TBARS. The number and diameter of retinal blood vessels, as morphometric parameters on digital retinal photography, was determined by using the ImageJ software. Student?s t-test was used as the statistical method for the evaluation of differences between the morphometric and blood test parameters. The significance of differences in morphometric parameters of retinal blood was establish by one-way ANOVA. Results. Significantly higher levels of parameters of oxidative stress (AOPP and TBARS) were in the group of patients with DR than in the controls. This difference was also present among the patients with mild and severe forms of DR (AOPP F 77.03, p < 0.001) (TBARS F 63.28, p < 0.001). The diameter of retinal blood vessels correlated with levels of AOPP, but only in patients with mild DR. Conclusion. Parameters of oxidative stress, AOPP and TBARS, may be important for the follow-up of DR. In early stages in diabetic retinopathy, AOPP can be a valuable biomarker.

Analysis of Oxidative Stress Markers in Glucose-6-Phoshate Dehydrogenase Deficient Umbilical Cord Blood Samples in Kampala, Uganda

PEDIATRICS ◽  
2016 ◽  
Vol 137 (Supplement 3) ◽  
pp. 392A-392A
Author(s):  
Paul S. Stadem ◽  
Megan Hilgers ◽  
Derrick Bengo ◽  
Susan Nabadda-Ndidde ◽  
Troy Lund
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers

Effect of different models of physical exercise on oxidative stress markers in mouse liver

2009 ◽  
Vol 34 (1) ◽  
pp. 60-65 ◽  
Author(s):  
Luciano A. da Silva ◽  
Cleber A. Pinho ◽  
Luis G.C. Rocha ◽  
Talita Tuon ◽  
Paulo C.L. Silveira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Physical Exercise ◽  
Mouse Liver ◽  
Citrate Synthase ◽  
Training Session ◽  
Thiobarbituric Acid ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Continuous Running

The aim of this study was to investigate the effect of different protocols of physical exercise on oxidative stress markers in mouse liver. Twenty-eight male CF1 mice (30–35 g) were distributed into 4 groups (n = 7) — untrained (UT), continuous running (CR), downhill running (D-HR), and intermittent running (IR) — and underwent an 8-week training program. Forty-eight hours after the last training session, the animals were killed, and their livers were removed. Blood lactate, creatine kinase, citrate synthase, thiobarbituric acid reactive species, carbonyl, superoxide dismutase (SOD), and catalase (CAT) activities were assayed. Results show a decrease in the level of lipoperoxidation and protein carbonylation in the CR and D-HR groups. SOD activity was significantly increased and CAT activity was reduced in the CR and D-HR groups. Our findings indicate that CR and D-HR may be important for decreasing oxidative damage and in the regulation of antioxidant enzymes (SOD and CAT) in the livers of trained mice.

scholarly journals Increased Trimethylamine N-Oxide Is Not Associated with Oxidative Stress Markers in Healthy Aged Women

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Robert Antoni Olek ◽  
Joanna Jolanta Samulak ◽  
Angelika Katarzyna Sawicka ◽  
Dace Hartmane ◽  
Solveiga Grinberga ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Carbonyls ◽  
Carnitine Supplementation ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Markers Of Oxidative Stress ◽  
White Blood Cell Counts

Increased plasma trimethylamine N-oxide (TMAO) levels have been associated with cardiovascular diseases (CVD). L-carnitine induces TMAO elevation in human blood, and thus, it has been suggested as developing atherosclerosis. The aim of this study was to determine the relation between selected markers of oxidative stress and plasma TMAO concentration induced by L-carnitine supplementation for 24 weeks in healthy aged women. Twenty aged women were supplemented during 24 weeks with either 1500 mg L-carnitine-L-tartrate (n=11) or isonitrogenous placebo (n=9) per day. Fasting blood samples were taken from antecubital vein. L-carnitine supplementation induced an increase in TMAO, but not in γ-butyrobetaine (GBB). Moreover, there were no significant changes in serum ox-LDL, myeloperoxidase, protein carbonyls, homocysteine, and uric acid concentrations due to supplementation. Significant reduction in white blood cell counts has been observed following 24-week supplementation, but not attributable to L-carnitine. Our results in healthy aged women indicated no relation between TMAO and any determined marker of oxidative stress over the period of 24 weeks. At the same time, plasma GBB levels were not affected by L-carnitine supplementation. Further clinical studies of plasma GBB level as a prognostic marker are needed.

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