Detection of Secondary Metabolite of Mycena pelianthina Growth in Various Liquid Medium

Mushrooms which is mostly belong to the phylum Basidiomycota have been recognized as functional foods and a source for the nutraceuticals. Genus Mycena is one of mushrooms, which has been investigated for its secondary metabolites. Mycena pelianthina produces pelianthinarubins A, pelianthinarubins B, muscarin, and epimuscarin from alkaloid group. The purposes of this research were to determine the effects of composition of medium and incubation time on production of mycelial biomass of M. pelianthina in liquid medium and to know the group of secondary metabolite compounds produced. This research was done experimentally using a Factorial Completely Randomized Design consisted of 12 treatments and three replications. The first factor was medium type, which were Mushroom Complete Medium (MCM), Potato Dextrose Yeast Broth (PDYB), and Yeast Malt Extract Medium (YM). The second factor was the incubation time consisting of 15, 20, 25, and 30 days. The observed main parameters were the dry weight of mycelial biomass and the secondary metabolite groups. The dried weights of mycelia were analyzed using analysis of variance (ANOVA), continued with Duncan test at a 95% confidence level. The best growth of M. pelianthina mycelium was produced on PDYB medium at incubation time of 20 days with an average mycelial dry weight of 0.92 g/100 mL. M. pelianthina contained secondary metabolite compound groups of alkaloids, terpenoids, and flavonoids.

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Effect of planting date and density on calendula and peppermint herbs

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v1i1.4241 ◽

2014 ◽

Vol 1 (1) ◽

pp. 25-29

Author(s):

Rahim Mohammadian ◽

Behnam Tahmasebpour ◽

Peyvand Samimifar

Keyword(s):

Mutual Effect ◽

Dry Weight ◽

Planting Date ◽

Mass Reduction ◽

Flower Number ◽

Planting Dates ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Total Maximum

A factorial experiment was conducted with a completely randomized design to evaluate the effects of planting date and density on calendula herbs and peppermint. It had 3 replicates and was done in Khosroshahr research farm, Tabriz in 2006. Under studied factors were: 3 planting dates (10 May, 25 May and 10 June) in 4 densities (25, 35, 45, 55) of the plant in square meters. The results of variance a nalysis showed that there was 1% probability significant difference between the effects of planting date and bush density on the leave number, bush height and the bush dry weight. But the mutual effect of the plant date in mentioned traits density was insignificant. Regarding the traits mean comparison, the total maximum dry weight was about the 55 bush density in mm. Also, the bush high density in mm causes the bush growth and its mass reduction. When there is the density grain, the flower number will increase due to bush grain in surface unit. Overall, we can conclude that 10 June planting and 45 bush density in mm is the most suitable items and results in favored production with high essence for these crops.

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Respon Akar Bibit Aren Genjah (Arenga pinnata) Di Pembibitan Pada Pemberian Dosis dan Interval Pupuk Organik Cair Nasa

Agrifarm : Jurnal Ilmu Pertanian ◽

10.24903/ajip.v7i1.368 ◽

2018 ◽

Vol 7 (1) ◽

pp. 28

Author(s):

Yetti Elidar

Keyword(s):

Organic Fertilizer ◽

Dry Weight ◽

Treatment Combination ◽

Root Dry Weight ◽

Randomized Design ◽

Dose Study ◽

Palm Sugar ◽

Wet Weight ◽

Completely Randomized Design ◽

Leaf Dry Weight

Research on the response of roots of palm sugar palm seeds (Arenga pinnata) in nurseries at doses and intervals of Nasa liquid organic fertilizer. Aims to determine the dosage, interval and combination of dosages and fertilization intervals with Nasa liquid organic fertilizer which can provide the best dry weight of the roots in the nursery. The research design used was a Completely Randomized Design (CRD) with 3x3 factorial experiments and each treatment was repeated 8 (eight) times, consisting of: the first factor was the treatment of POC Nasa dose in a concentration of 3 cc POC Nasa per liter of water (D) consists of 3 levels, namely: d1 = 300 ml POC Nasa, d2 = 400 ml POC Nasa, d3 = 500 ml POC Nasa, while the second factor is the treatment of POC Nasa Interval (I) consisting of 3 levels, namely: i1 = 2 once a week, i2 = once every 3 weeks, i3 = once every 4 weeks. The results of the POC Nasa dose study had a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level are: d2 (400 ml of Nasa liquid organic fertilizer), the interval of liquid organic fertilizer Nasa has a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level is: i1 (once every 2 weeks). The treatment combination has no significant effect on all parameters.

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Isolation and Characterization of Yeasts from Rumen Fluids for Potential Use as Additives in Ruminant Feeding

Veterinary Sciences ◽

10.3390/vetsci8030052 ◽

2021 ◽

Vol 8 (3) ◽

pp. 52

Author(s):

Chanon Suntara ◽

Anusorn Cherdthong ◽

Metha Wanapat ◽

Suthipong Uriyapongson ◽

Vichai Leelavatcharamas ◽

...

Keyword(s):

Incubation Time ◽

Cellulase Activity ◽

The Other ◽

Pichia Kudriavzevii ◽

Factor B ◽

Yeast Strains ◽

Randomized Design ◽

Holstein Friesian ◽

The Media ◽

Completely Randomized Design

Saccharomyces cerevisiae is a yeast strain often used to improve the feed quality of ruminants. However, S. cerevisiae has limited capacity to provide biomass when inoculated with carbon sources and a low ability to produce cellulase enzymes. Here, we hypothesized that yeast in the rumen produces a large amount of biomass and could release cellulase enzymes to break down fiber content. Therefore, the aim of this study was to screen, isolate and identify yeast from the rumen fluids of Holstein Friesian steers and measure the efficiency of biomass production and cellulase activity. A fermentation medium containing sugarcane molasses as a carbon source and urea as a nitrogen source was optimized. Two fistulated–crossbred Holstein Friesian steers averaging 350 ± 20 kg body weight were used to screen and isolate the ruminal yeast. Two experiments were designed: First, a 12 × 3 × 3 factorial was used in a completely randomized design to determine biomass and carboxymethyl cellulase activity. Factor A was the isolated yeast and S. cerevisiae. Factor B was sugarcane molasses (M) concentration. Factor C was urea (U) concentration. In the second experiment, potential yeasts were selected, identified, and analyzed for 7 × 4 factorial use in a completely randomized design. Factor A was the incubation times. Factor B was the isolated yeast strains, including codes H-Khon Kaen University (KKU) 20 (as P. kudriavzevii-KKU20), I-KKU20 (C. tropicalis-KKU20), and C-KKU20 (as Galactomyces sp.-KKU20). Isolation was imposed under aerobic conditions, resulting in a total of 11 different colonies. Two appearances of colonies including asymmetric colonies of isolated yeast (indicated as A, B, C, E, and J) and ovoid colonies (coded as D, F, G, H, I, and K) were noted. Isolated yeast from the rumen capable of providing a high amount of biomass when inoculant consisted of the molasses 15% + urea 3% (M15 + U3), molasses 25% + urea 1% (M25 + U1), molasses 25% + urea 3% (M25 + U3), and molasses 25% + urea 5% (M25 + U5) when compared to the other media solution (p < 0.01). In addition, 11 isolated biomass-producing yeasts were found in the media solution of M25 + U1. There were 4 isolates cellulase producing yeasts discovered in the media solution of M25 + U1 and M25 + U5 whereas molasses 5% + urea 1% (M5 + U1), molasses 5% + urea 3% (M5 + U3), molasses 5% + urea 5% (M5 + U5), molasses 15% + urea 1% (M15 + U1), molasses 15% + urea 3% (M5 + U3), and M25 + U3 were found with 2, 3, 1, 2, 1, and 2 isolates, respectively. Ruminal yeast strains H-KKU20, I-KKU20, and C-KKU20 were selected for their ability to produce biomass. Identification of isolates H-KKU20 and I-KKU20 revealed that those isolates belonged to Pichia kudriavzevii-KKU20 and Candida tropicalis-KKU20 while C-KKU20 was identified as Galactomyces sp.-KKU20. Two strains provided maximum cell growth: P. kudriavzevii-KKU20 (9.78 and 10.02 Log cell/mL) and C. tropicalis-KKU20 (9.53 and 9.6 Log cells/mL) at 60 and 72 h of incubation time, respectively. The highest ethanol production was observed in S. cerevisiae at 76.4, 77.8, 78.5, and 78.6 g/L at 36, 48, 60, and 72 h of incubation time, respectively (p < 0.01). The P. kudriavzevii-KKU20 yielded the least reducing sugar at about 30.6 and 29.8 g/L at 60 and 72 h of incubation time, respectively. The screening and isolation of yeasts from rumen fluids resulted in 11 different yeasts being obtained. The potential yeasts discovered in the rumen fluid of cattle were Pichia kudriavzevii-KKU20, Candida tropicalis-KKU20, and Galactomyces sp.-KKU20. P. kudriavzevii-KKU20 had higher results than the other yeasts in terms of biomass production, cellulase enzyme activity, and cell number.

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Perlakuan Berbagai Dosis Pupuk ZA Pertumbuhan Dan Hasil Tanaman Bayam Merah (Amaranthus gangeticus)

Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis ◽

10.30737/agrinika.v5i2.1946 ◽

2021 ◽

Vol 5 (2) ◽

pp. 180

Author(s):

Edy Kustiani ◽

Mariyono Mariyono ◽

Bela Citra Ayuningtyas

Keyword(s):

Analysis Of Variance ◽

Nutritional Value ◽

Environmental Design ◽

Dry Weight ◽

Growth And Yield ◽

Dose Treatment ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

The Ideal

Red spinach (Amaranthus gangeticus) is one of the most popular vegetables because of its high nutritional value and easy cultivation. Efforts to increase the production of red spinach are to fertilize to meet the needs of both macro and micronutrients. This study aimed to determine the effect of ZA fertilizer dose on the growth and yield of red spinach. The environmental design used was a non-factorial, completely randomized design with four replications. The factors tested were the dose of ZA fertilizer which consisted of 6 levels, namely: without ZA fertilizer (D0); 0.4 gr/polybag (D1); 0.8 gr/polybag (D2); 1.2 gr/polybag (D3); 1.6 gr/polybag (D4); 2 gr/polybag (D5). The data obtained were analyzed using analysis of variance, and if the results were significantly different, the BNT test was continued at a 5% level. The growth and yield of red spinach showed significant differences due to the ZA fertilizer dose treatment. The ZA fertilizer dose treatment of 1.2 g/polybag (D3) resulted in the highest plant height, the number of leaves, and leaf area of red spinach at 14, 21, and 28 days after planting. The ZA fertilizer dose treatment of 1.2 g/polybag (D3) resulted in the highest wet and dry weight plants of 49.15 and 4.38 grams, respectively. The ZA 1.2 gr/polybag dose is the ideal dose to increase the productivity of red spinach.Bayam merah (Amaranthus gangeticus) termasuk sayuran penting yang disukai masyarakat karena bernilai gizi tinggi dan mudah pembudidayaannya. Upaya peningkatan hasil bayam merah salah satunya dengan pemupukan untuk menambah nutrisi yang dibutuhkan tanaman. Tujuan dari penelitian ini adalah mempelajari respon pertumbuhan dan hasil tanaman bayam merah pada perlakuan dosis pupuk ZA. Pelaksanaan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) satu faktor. Faktor yang diujikan adalah dosis pupuk ZA, terdiri 6 taraf, yaitu: tanpa pupuk ZA (D0); 0.4 gr/polybag (D1); 0.8 gr/polybag (D2); 1.2 gr/polybag (D3); 1.6 gr/polybag (D4); 2 gr /polybag (D5). Percobaan dilakukan dengan ulangan sebanyak 4 kali. Analisis data menggunakan analysis of variance (ANOVA), apabila berbeda nyata dilanjutkan uji Beda Nyata Terkecil (BNT) taraf 5%. Perlakuan dosis pupuk ZA berpengaruh nyata terhadap pertumbuhan dan hasil tanaman bayam merah. Perlakuan dosis pupuk ZA 1.2 gr/polybag (D3) menghasilkan tinggi, jumlah dan luas daun tanaman bayam merah tertinggi pada pengamatan umur 14, 21, dan 28 HST. Dosis pupuk ZA 1.2 gr/polybag (D3) juga menunjukkan berat basah dan berat kering tanaman tertinggi sebesar 49.15 dan 4.38 gram. Dosis ZA 1.2 gr/polybag merupakan dosis ideal untuk meningkatkan produktivitas tanaman bayam merah.

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Germination test of Eucalyptus phaeotricha seeds

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v22n9p653-657 ◽

2018 ◽

Vol 22 (9) ◽

pp. 653-657 ◽

Cited By ~ 1

Author(s):

Carlos E. Affonso ◽

Givanildo Z. da Silva ◽

Tatiane S. Jeromini ◽

Cibele C. Martins

Keyword(s):

Dry Weight ◽

Water Volume ◽

Conduction Time ◽

Germination Test ◽

Randomized Design ◽

Temperature Regimes ◽

Seed Analysis ◽

Completely Randomized Design ◽

Mean Time ◽

Temperature Conduction

ABSTRACT The rules for seed analysis do not have recommendations for conducting the germination test of Eucalyptus phaeotricha seeds. Thus, the aim of this study was to determine the best substrate, temperature, conduction time, and substrate moistening level for the germination test of E. phaeotricha seeds. First count of germination, germination, germination speed index and mean time of germination were evaluated. The first experiment was carried out in a completely randomized design, in a factorial scheme (6 x 2), corresponding to six temperature regimes (15, 20, 25, 30, 35 °C and 20-30 °C) and two substrates (paper and sand) with four replicates, and the means were compared by Tukey test at 0.05 probability level. In the second experiment, the germination test was installed with the most favorable substrate and temperature identified in the previous step, evaluating substrate moistening levels equivalent to 1.0, 1.5, 2.0, 2.5 and 3.0 times the dry weight of the substrate; due to the quantitative nature of the treatments, the data were subjected to regression analysis and curve fitting. The germination test of E. phaeotricha seeds should be conducted at 30 °C on paper, moistened with a water volume of 2.2 times the dry weight of the paper, with initial and final counts at 6 and 14 days after sowing, respectively.

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PENGARUH INTENSITAS PEMOTONGAN TERHADAP PRODUKTIVITAS Indigofera zollingeriana

ZOOTEC ◽

10.35792/zot.40.2.2020.29881 ◽

2020 ◽

Vol 40 (2) ◽

pp. 665

Author(s):

Ivan H.G.M. Wagiu ◽

Ch.L. Kaunang ◽

M.M. Telleng ◽

W.B Kaunang

Keyword(s):

Dry Weight ◽

Randomized Design ◽

Optimal Productivity ◽

Indigofera Zollingeriana ◽

Completely Randomized Design ◽

The Difference ◽

Cutting Intensity

THE EFFECT OF DEFOLIATION INTENSITY ON PRODUCTIVITY OF Indigofera zollingeriana. This research aims to know the effect of defoliaion intensity on productivity (fresh production, dry weight production, proportion of leaves and stems) and to know the defoliation intensity with optimal productivity of forage Indigofera zollingeriana. This research was conducted in December 2019 until March 2020. It consisted of defoliation intensity at 75cm (T75), 100cm (T100), 125cm (T125), and 150cm (T150). The design used in this experiment is Completely Randomized Design. The variables in this research were 1. fresh production leaves and stems; 2. dry weight production leaves and stems; 3. the proportion of leaves and stems dry weight production of forages Indigofera zollingeriana. The results showed that the difference of intensity defoliation had a significantly effect (P<0,01) on productivity (fresh production, dry weight production, proportion of leaves and stems) forage Indigofera zollingeriana. Defoliation intensity 75 cm have significantly (P<0,01) fresh and dry weight leaf of Indigofera zollingeriana than hight defoliation 125 cm and 150 cm. Intensity defoliation 125 cm have significantly (P<0,01) leaf-stem ratio of Indigofera zollingeriana than any other. It conclude that intensity defoliation 125 cm have highly productivity. Keywords: Indigofera zollingeriana, cutting intensity, productivity

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Pengaruh Campuran Pasir dan Serat Aren pada Kailan (Brassica oleraceae) dengan Hidroponik Substrat

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v16i1.18908 ◽

2014 ◽

Vol 16 (1) ◽

pp. 19

Author(s):

Samanhudi Samanhudi ◽

Dwi Hardjoko ◽

Riandy Adhitya

Keyword(s):

Wood Fiber ◽

Dry Weight ◽

Fresh Weight ◽

Randomized Design ◽

The Media ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Brassica Oleraceae ◽

Media Mix ◽

Leaf Greenness

Wood fiber waste of arenga produced from starch palm industry. It is not handled properly so that could be potentially cause problems for the surrounding community. Wood fiber waste of arenga is potentially used as a medium for hydroponics. The waste can be utilized to get a new composition of hydroponic media. This study aims to determine the effect of a mixture of sand with arenga fiber waste. This research was carried out by using a mixture of sand and arenga wood fiber waste as a medium. This is an experimental study using a completely randomized design. This study aims to determine the effect of a mixture of sand and arenga fiber waste for kailan growth. The results showed that the mixture of sand and arenga wood fiber waste has no effect on kailan growth. The combination of mixtures of sand and arenga fiber waste showed no interaction. Observations on plants such as plant height, number of leaves, leaf greenness levels, the fresh weight of crop stover and stover dry weight of the plant, showed a row of the highest results in the media mix washed sand, washed sand and rice husk

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Toleransi Plasma Nutfah Padi Lokal terhadap Salinitas

Buletin Plasma Nutfah ◽

10.21082/blpn.v23n1.2017.p51-58 ◽

2018 ◽

Vol 23 (1) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Tintin Suhartini ◽

Try Zulchi

Keyword(s):

Plant Height ◽

Salinity Tolerance ◽

Negative Correlation ◽

Dry Weight ◽

Gene Bank ◽

Dead Leaf ◽

Randomized Design ◽

Highly Sensitive ◽

Number Of Leaves ◽

Completely Randomized Design

AbstractThe research was aimed to screen the local rice germplasms for salinity tolerance. The 104 local rice germplasms were derived of collection of Gene Bank of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) were tested to salinity. The activities was carried out in greenhouse of ICABIOGRAD. The 15 day old seedlings were planted in pots soil contained NaCl 0.4%. One accession of rice was planted in 2 pots, each pot contained 4 seedlings. Observation of plant symptom was conducted after 1 month planted. The experiment was carried out in a Completely Randomized Design with 2 replications. The results of the analysis showed there were different responsses among rice accesions to the treatment. Salinity caused dry and dead leaves, there were very significant differences between the varieties for plant height, dry weight plant, number of total leaves, and number of green leaves. There were a high and negative correlation of plant height and total number of leaves with percentage of dead leaves. The result showed 21 accesions were tolerant to moderately&#8722;tolerant, while Pokkali as a tolerant check, most tolerant. There were two accessions were tolerant with dead leaf percentage <50%, i.e. Tjempo Brondol (reg. 5800) and Gembira Putih (reg. 20602), whereas Pokkali as resistant check had low dead leaf percentage (16.9%) and 19 accessions were moderately-tolerant with dead leaf percentage <70%, and 82 accesions were sensitive to highly-sensitive.AbstrakPenelitian ini bertujuan untuk menyaring plasma nutfah padi yang memiliki sifat toleran terhadap salinitas. Sejumlah 104 plasma nutfah padi lokal yang berasal dari koleksi Bank Gen BB Biogen diuji terhadap salinitas. Kegiatan dilakukan di rumah kaca BB Biogen. Bibit padi berumur &plusmn;15 hari ditanam pada pot berisi tanah yang sudah dilumpurkan dengan larutan garam NaCl 0,4% (4.000 ppm). Satu aksesi padi ditanam pada 2 pot, masing masing pot berisi 4 tanaman. Setelah berumur 4 minggu dari tanam dilakukan pengamatan meliputi tinggi tanaman, bobot kering akar, bobot kering tanaman, jumlah daun total, jumlah daun hijau, dan jumlah daun mati. Pengujian menggunakan Rancangan Acak Lengkap dengan 2 ulangan. Hasil analisis varians menunjukkan terdapat perbedaan respons plasma nutfah padi terhadap perlakuan salin yang diberikan. Perbedaan sangat nyata terdapat pada tinggi tanaman, bobot kering tanaman, jumlah daun total, dan jumlah daun hijau. Terdapat korelasi yang tinggi dan negatif pada tinggi tanaman dan jumlah daun total dengan persentase daun mati. Hasil pengujian telah terpilih 21 aksesi plasma nutfah padi toleran hingga agak toleran, sedangkan Pokkali sebagai cek sangat toleran. Dua aksesi yang toleran dengan persentase daun mati <50%, yaitu Tjempo Brondol (reg. 5800) dan Gembira Putih (reg. 20602), Pokkali sebagai varietas kontrol dengan tingkat persentase daun mati 16,9%, sedangkan 19 aksesi termasuk kelompok agak toleran dengan persentase daun mati <70%, dan 82 aksesi termasuk kelompok peka hingga sangat peka.

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PERTUMBUHAN STEK KOPI ROBUSTA (Coffea canephora) DENGAN PEMBERIAN EKSTRAK BAWANG MERAH

Jurnal Media Pertanian ◽

10.33087/jagro.v6i1.108 ◽

2021 ◽

Vol 6 (1) ◽

pp. 43

Author(s):

Yulistiati Nengsih ◽

Aditya Deska Wahyu

Keyword(s):

Dry Weight ◽

Coffea Canephora ◽

Shoot Length ◽

Distilled Water ◽

Onion Extract ◽

Robusta Coffee ◽

Randomized Design ◽

The World ◽

Treatment Factor ◽

Completely Randomized Design

Indonesia is one of the largest coffee producing and exporting countries in the world. Most of the production is robusta variety. Coffee is the fourth largest foreign exchange earner for Indonesia after palm oil, rubber and cocoa and is a source of income for the community. Coffee can be developed in a generative (seed) and vegetative way, including using cuttings. Efforts to accelerate the formation of roots in cuttings can be done by using natural growth regulators, one of which is shallots which contain auxins. The purpose of this study was to examine the effect of shallot extract on the growth of robusta coffee cuttings (Coffea canephora). This research was conducted from December 2019 to February 2020 at Campus II, Batanghari University (Pijoan). The design used in this study was a completely randomized design (CRD) with one treatment factor, namely shallot extract including; .k0 (without giving onion extract), k1 (250 g of shallots in 1 liter of distilled water), k2 (500 g of shallots in 1 liter of distilled water), k3 (750 g of shallots in 1 liter of distilled water), and k4 (1000 g of shallots in 1 liter of distilled water). Each stage of the experiment was repeated 3 times, so that there were 15 experimental plots, for each experiment there were 10 cuttings with a total of 150 cuttings. The observed data were analyzed using analysis of variance followed by the DNMRT test at α 5%. From the immersion treatment in 750 g of shallot extract in 1 liter of distilled water (k3), there was an increase in the percentage of live cuttings 47.37%, the percentage of shoots and roots growth of 83.51%, 106.06% of shoot length, and 98.03% of roots. , and the root dry weight of 225.27% compared without giving onion extract (k0).

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ASSESSING THE NITROGEN FIXATION ABILITIES OF NEWLY DEVELOPED PHASEOLUS VULGARIS LINES

HortScience ◽

10.21273/hortsci.27.6.662e ◽

1992 ◽

Vol 27 (6) ◽

pp. 662e-662

Author(s):

Dyremple B. Marsh ◽

Wayne McLaughlin ◽

James S. Beaver

Keyword(s):

Phaseolus Vulgaris ◽

Dry Weight ◽

Tropical Regions ◽

Breeding Lines ◽

Shoot Dry Weight ◽

Greenhouse Study ◽

Kidney Beans ◽

Randomized Design ◽

Red Kidney Bean ◽

Completely Randomized Design

Methods to improve the grain yield of red kidney bean without the addition of commercially fixed nitrogen will have significant benefits to farmers in Jamaica and other tropical regions. Red kidney beans provide a major portion of the dietary protein for most families in these regions. Our experimental objective was to evaluate the nitrogen fixing capabilities of several breeding lines of Phaseolus vulgaris when inoculated with Rhizobium strains isolated from Jamaican soils. Surface sterilized seeds of 11 Phaseolus lines were inoculated with inoculum prepared from 5 day old Rhizobium YEM mixture. Rhizobium used were T2 and B17 from Jamaica and UMR 1889. The greenhouse study was arranged as a completely randomized design. Bean lines 9056-101, 9056-98B, 8954-5 and 8954-4 showed improved nodulation and N2 fixation when inoculated with UMR 1899. The combination of breeding line 8954-5 and Rhizobium strain B17 produced the highest nodule number and shoot dry weight of 193 and 0.72 g, respectively. The Rhizobium strain B17showed some ability to compete successfully for nodule sites against known effective strains.

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