scholarly journals Biologically active components of medicinal plants in the fermented milk product «Dyvosyl»

Author(s):  
T. Kutsyk

The article is devoted to the scientific substantiation of technological solutions, which consists in the enrichment of dairy basis of bacterial concentrate of kefir grains and functional ingredients of medicinal plants to create the original composition of the product introduced medicinal plant with diverse therapeutic areas — functional sour-milk product «Dyvosyl». Experimental work was conducted at the Department of biotechnology of the Institute of Food Resources NAAS. Set doses of functional ingredients, bacterial concentrate of kefir grains and according to the research are for bacterial concentrate — 5 g/t of dry extract of root of mallow medicinal — 120 g/t, dry extract of rootstock and roots of inula — 160 g/t, of essential oil peppermint — 2.5 g/t. The use of kefir grains in complex with functional ingredients allows us to obtain a fermented milk product with a significant amount of biologically active microorganisms (total number lactic acid bacteria (LAB) ((0.8÷1.01)±0.05)×109 CFU/g), increased levels of free amino acids (up to 12.4% of the total number) and a minimal amount of cholesterol ((61.2±0.1)%). It has been determined that for dry extracts we should use initial dissolution, thermal treatment at a temperature of (92±2)°C, endurance (15±2) minutes and the introduction of a solution of the extracts before fermentation of the milk. The fermentation should be carried out at a temperature of (25±1)°C for (8–9) hours to raise titrated acidity (80±5)°T, Essential oil of peppermint to make only after the process of aging of the product. The use of functional ingredients has allowed extending the shelf life to 12 days. Product originality and novelty of technological solutions of production of the product is confirmed by the patent of Ukraine for invention Method of production of functional fermented milk product «Dyvosyl» (Pat. No. 97772 Ukraine, IPC AS 9/13(2006.01), AC/127 (2006.01), pub. 12.03.2012 R., bull. No. 5).

2017 ◽  
Vol 55 (1) ◽  
pp. 252-257 ◽  
Author(s):  
Mykola Kukhtyn ◽  
Olena Vichko ◽  
Yulia Horyuk ◽  
Olga Shved ◽  
Volodymyr Novikov

2020 ◽  
Vol 8 (2) ◽  
pp. 192 ◽  
Author(s):  
Fatemeh Nejati ◽  
Stefan Junne ◽  
Peter Neubauer

Milk kefir is a traditional fermented milk product whose consumption is becoming increasingly popular. The natural starter for kefir production is kefir grain, which consists of various bacterial and yeast species. At the industrial scale, however, kefir grains are rarely used due to their slow growth, complex application, bad reproducibility and high costs. Instead, mixtures of defined lactic acid bacteria and sometimes yeasts are applied, which alter sensory and functional properties compared to natural grain-based milk kefir. In order to be able to mimic natural starter cultures for authentic kefir production, it is a prerequisite to gain deep knowledge about the nature of kefir grains, its microbial composition, morphologic structure, composition of strains on grains and the impact of environmental parameters on kefir grain characteristics. In addition, it is very important to deeply investigate the numerous multi-dimensional interactions among different species, which play important roles on the formation and the functionality of grains.


2021 ◽  
pp. 8-12
Author(s):  
Елизавета Владимировна Залисская

Полноценное и здоровое питание является неотъемлемым фактором, прямо влияющим на развитие организма и общего здоровья человека. В мире все больший интерес представляет разработка и производство функциональных продуктов питания. Среди них сегмент молочных продуктов считается одним из самых быстрорастущих и перспективных. Особый интерес для научного сообщества представляют природные ассоциации, нашедшие применение в пищевой промышленности. Кефирный грибок - естественная спонтанная ассоциация нескольких видов микроорганизмов, широко используемая для производства кисломолочного продукта кефир. В состав кефира входят более 50 видов различных молочнокислых бактерий и дрожжей, что отличает его от других пробиотических продуктов. Исследователи, занимающиеся изучением кефира, открывают новые, ранее неизвестные его свойства. Большое количество исследований проводится по пробиотическим свойствам кефира, в частности широко изучаются его антимикробные, противоопухолевые, иммуномодулирующие, противовоспалительные и ранозаживляющие действия. Подобные пробиотические свойства кефира связывают в основном с присутствием лактобацилл в составе кефирных грибков. Также в ряде исследований указывается, что кефир обладает более выраженной антиоксидантной активностью, чем известный антиоксидант витамин Е. В статье описаны такие механизмы антиоксидантной активности бактерий, входящих в состав кефира, как ферментативная защита клеток, хелатирующая способность лактобактерий и иные механизмы, нейтрализующие действия свободных радикалов. Также собраны и представлены наиболее популярные методы определения антиоксидантной активности, такие как ABTS, DPPH, ORAC, FRAP. Приведены результаты исследований кефира, приготовленного с использованием молока различных сельскохозяйственных животных. A complete and healthy diet is an integral factor that directly affects the development of the body and general human health. The development and production of functional food products are of increasing interest in the world. Among them, the segment of dairy products is considered one of the fastest-growing and most promising. Natural associations that have found application in the food industry are of particular interest to the scientific community. Kefir grains are a natural spontaneous association of several types of microorganisms, widely used for the production of the fermented milk product kefir. Kefir contains more than 50 different species of lactic acid bacteria and yeast, which distinguishes it from other probiotic products. Researchers studying kefir are finding new and hitherto unknown properties. A large number of studies are carried out on the probiotic properties of kefir, in particular, its antimicrobial, antitumor, immunomodulating, anti-inflammatory, and wound-healing effects are widely studied. Such probiotic properties of kefir are mainly associated with the presence of lactobacilli in kefir grains. Several studies indicate that kefir has a more pronounced antioxidant activity than the well-known antioxidant vitamin E. The article describes mechanisms of the antioxidant activity of bacteria that make up kefir, such as enzymatic antioxidant protection of cells, the chelating ability of lactobacilli, and other mechanisms of antioxidant activity. Also collected and presented the most popular methods for determining antioxidant activity, such as ABTS, DPPH, ORAC, FRAP. The results of studies of kefir prepared using milk from various farm animals are presented.


2020 ◽  
pp. 48-50
Author(s):  
I.V. Rozhkova ◽  
◽  
A.V. Begunova ◽  
T.I. Shirshova ◽  
Yu. I. Krysanova ◽  
...  

2018 ◽  
pp. 54-55
Author(s):  
A.V. Begunova ◽  
◽  
I.V. Rozhkova ◽  
T.A. Raskoshnaya ◽  
T.I. Shyrshova ◽  
...  

2021 ◽  
Vol 9 (16) ◽  
pp. 180-191
Author(s):  
Аlla Solomon ◽  
◽  
Iryna Bernyk ◽  
Мariana Bondar ◽  
◽  
...  

State policy in the field of healthy nutrition is a timely and vital, because inadequate nutrition for the physical needs of the body poses a threat to national security today. The article analyzes the state of nutrition of the population of Ukraine in recent years, which is marked by negative trends, both in relation to energy adequacy and in relation to the chemical composition of diets. It is proven that the harm to health is caused by insufficient intake of vitamins, minerals and trace elements, resulting in reduced physical and mental performance, resistance to various diseases, increased negative effects on the body of adverse environmental conditions, harmful production factors, nervous and emotional stress and stress. The purpose of this work is to scientifically substantiate the composition of a fermented milk product enriched with sprouted barley grains and rosehip syrup, which allows improving the structure of the human nutrition through the use of functional ingredients and facilitating the adaptation of the human body to adverse external conditions. Experts associate the positive effect of fermented milk products for the human body with the appearance in them of physiologically active functional components, which are also useful in a physiological form. The value of fermented milk products in functional nutrition is determined primarily by the unique composition of microflora, food and biological value of products. Speaking about the nutritional value of fermented milk products, it is necessary to consider in detail the nutritional substances that make up its composition. In the lifr process of lactic acid bacteria, a complex of biologically active substances (enzymes, lactic and acetic acids, antibiotic substances) is accumulating. Dietary fermented milk products improve metabolism, stimulate the secretion of gastric juice, stimulate the appetite. Improving the health of the human body and ensuring its active life through the use of fermented milk products with functional properties is a new promising direction in medicine and nutrition, as its integral part.


2001 ◽  
Vol 64 (4) ◽  
pp. 462-469 ◽  
Author(s):  
A. JAGANNATH ◽  
M. N. RAMESH ◽  
M. C. VARADARAJ

The increasing popularity of traditional milk-based foods has placed emphasis on the need for microbial safety in food-chain establishments, as there are ample possibilities for foodborne pathogens to occur as postprocessing contaminants. The behavioral pattern of an enterotoxigenic strain of Escherichia coli D 21 introduced as a postprocessing contaminant in shrikhand, a traditional sweetened lactic fermented milk product, was studied with variables of initial inoculum (4.3, 5.3, and 6.3 log10 CFU/g), storage temperature (4, 10, and 16°C), and storage period (4, 9, and 14 days). During storage of shrikhand prepared individually with Lactobacillus delbruecki ssp. bulgaricus CFR 2028 and Lactococcus lactis ssp. cremoris B-634, there was a steady decrease in the viable count of E. coli that was proportional to the initial inoculum of E. coli introduced into shrikhand. The data were subjected to multivariate analysis, and equations were derived to predict the behavior of E. coli in shrikhand. The predicted values for the probable survival of E. coli showed good agreement with the experimental values with a majority of these predictions being fail-safe. The values of statistical indices showed that the model fits ranged between extremely good and satisfactory. Response surface plots were generated to describe the behavioral pattern of E. coli. The derived models and response surface plots enabled prediction of the survival of E. coli in shrikhand as a function of initial inoculum levels, storage temperatures, and storage periods of shrikhand. These predictions were valid within the limits of the experimental variables used to develop the model.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Anne-Sophie Alvarez ◽  
Julien Tap ◽  
Isabelle Chambaud ◽  
Stéphanie Cools-Portier ◽  
Laurent Quinquis ◽  
...  

Abstract Many clinical studies have evaluated the effect of probiotics, but only a few have assessed their dose effects on gut microbiota and host. We conducted a randomized, double-blind, controlled intervention clinical trial to assess the safety (primary endpoint) of and gut microbiota response (secondary endpoint) to the daily ingestion for 4 weeks of two doses (1 or 3 bottles/day) of a fermented milk product (Test) in 96 healthy adults. The Test product is a multi-strain fermented milk product, combining yogurt strains and probiotic candidate strains Lactobacillus paracasei subsp. paracasei CNCM I-1518 and CNCM I-3689 and Lactobacillus rhamnosus CNCM I-3690. We assessed the safety of the Test product on the following parameters: adverse events, vital signs, hematological and metabolic profile, hepatic, kidney or thyroid function, inflammatory markers, bowel habits and digestive symptoms. We explored the longitudinal gut microbiota response to product consumption and dose, by 16S rRNA gene sequencing and functional contribution by shotgun metagenomics. Safety results did not show any significant difference between the Test and Control products whatever the parameters assessed, at the two doses ingested daily over a 4-week-period. Probiotic candidate strains were detected only during consumption period, and at a significantly higher level for the three strains in subjects who consumed 3 products bottles/day. The global structure of the gut microbiota as assessed by alpha and beta-diversity, was not altered by consumption of the product for four weeks. A zero-inflated beta regression model with random effects (ZIBR) identified a few bacterial genera with differential responses to test product consumption dose compared to control. Shotgun metagenomics analysis revealed a functional contribution to the gut microbiome of probiotic candidates.


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