scholarly journals THE INHIBITORY AND DESTRUCTIVE ACTION OF THE SILVER NANOPARTICLE PREPARATION ON BIOFILMS FORMED BY CLINICALLY RELEVANT MICROORGANISMS

2019 ◽  
Vol 18 (3(69)) ◽  
pp. 56-70
Author(s):  
M. A. Sukhina ◽  
Yu. A. Shelygin ◽  
A. Yu. Piyadina ◽  
N. B. Feldman ◽  
M. A. Ananyan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Average Diameter ◽  
Bacterial Films ◽  
Transmission Electron ◽  
Nanoparticle Preparation ◽  
Additional Stabilization ◽  
Initial Culture ◽  
Planktonic Form ◽  
Dioctyl Sodium Sulfosuccinate

AIM: to obtain and investigate the activity of silver nanoparticles stabilized with arabinogalactan in relation to clinically relevant strains of filmforming microorganisms. MATERIALS AND METHODS: silver nanoparticles were obtained by reduction from silver nitrate in the presence of arabinogalactan with additional stabilization with dioctyl sodium sulfosuccinate. The shape and size of the nanoparticles were determined by the method of transmission electron microscopy, the zeta potential by the method of electrophoretic light scattering. The study of the effect of the nanoparticles on biofilm formation was carried out on 17 clinically relevant strains of bacteria isolated from blood culture and the clinical biomaterial of postoperative patients. RESULTS: the silver nanoparticles with an average diameter of 11.4 nm and a zeta potential of –24 mV were obtained. The minimum inhibitory concentration of the nanoparticles in relation to planktonic form of bacteria was 120 µg/ml; the use of the drug at a concentration of 100 µg/ml reduced the amount of CFU by 7 orders of magnitude compared with the initial culture. The study of the effect of silver nanoparticles on the formation of biofilms showed that, in the presence of the drug, the growth of biofilms was significantly reduced; at a drug concentration of 150 µg/ml, the growth of bacterial films was completely suppressed. Incubation of the formed daily biofilms with the silver nanoparticles in the concentration range from 150 to 120 µg/ml for 48 h resulted in the partial or complete destruction of the biopolymer matrix. CONCLUSION: the studied preparation of silver nanoparticles has a great potential for use in the treatment of infectious diseases caused by biofilm forming microorganisms.

Citrate Stabilized Silver Nanoparticles

2011 ◽  
Vol 3 (3) ◽  
pp. 15-28 ◽  
Author(s):  
Nabraj Bhattarai ◽  
Subarna Khanal ◽  
Pushpa Raj Pudasaini ◽  
Shanna Pahl ◽  
Dulce Romero-Urbina
Keyword(s):  
Crystal Structure ◽  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Colloidal Solution ◽  
Measurement Techniques ◽  
Structural Defects ◽  
Potential Measurement ◽  
Transmission Electron

Citrate stabilized silver (Ag) colloidal solution were synthesized and characterized for crystallographic and surface properties by using transmission electron microscopy (TEM) and zeta potential measurement techniques. TEM investigation depicted the size of Ago ranges from 5 to 50 nm with smaller particles having single crystal structure while larger particles with structural defects (such as multiply twinned, high coalescence and Moire patterns). ?-potential measurement confirms the presence of Ag+ in nAg stock solution. The shift in ?-potential measurement by +25.1 mV in the filtered solution suggests the presence of Ag+ in Ago nanoparticles.

Effect of Laser Fluence on Siliver Nanoparticles Colloid

2011 ◽  
Vol 415-417 ◽  
pp. 747-750
Author(s):  
Bing Xu ◽  
Ren Guo Song ◽  
Chao Wang
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Laser Ablation ◽  
Laser Fluence ◽  
Pulsed Laser ◽  
Pulsed Laser Ablation ◽  
Average Diameter ◽  
Distilled Water ◽  
Transmission Electron ◽  
Silver Target

In order to study the effects of laser fluence on silver nanoparticles colloid, the silver nanoparticles colloid was prepared by pulsed laser ablation of silver target for 10min in distilled water at different laser fluence. The particles size，morphologies and absorption spectroscopy of the obtained nanoparticles colloid were characterized by ultraviolet to visible (UV-Vis) spectrometer and transmission electron microscopy (TEM), the average diameter and its distribution were analyzed by Image-ProPlus software. The results shown that the average diameter of the silver nanoparticles prepared at the laser fluence of 4.2J/cm2 was the smallest (D=17.54nm), also, the distribution of particle size was narrowest (=36.86nm) and the morphologies were more homogeneous. It was confirmed that the nanoparticles size and shape could be controlled by pulsed laser ablation parameters.

scholarly journals Investigation of the Larvicidal Potential of Silver Nanoparticles againstCulex quinquefasciatus: A Case of a Ubiquitous Weed as a Useful Bioresource

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Elijah T. Adesuji ◽  
Omolara O. Oluwaniyi ◽  
Haleemat I. Adegoke ◽  
Roshila Moodley ◽  
Ayomide H. Labulo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Volume Ratio ◽  
Average Diameter ◽  
Environmentally Benign ◽  
X Ray ◽  
Transmission Electron ◽  
Aqueous Leaf Extract ◽  
Temperature Time ◽  
Filariasis Vector

Biosynthesized silver nanoparticles (AgNPs) usingCassia hirsutaaqueous leaf extract were reported in this study. The synthesis was optimized by measuring various parameters such as temperature, time, volume ratio, and concentration. The surface plasmon resonance at 440 nm for 30°C and 420 nm for both 50°C and 70°C measured using the UV-Vis spectrophotometer confirmed the formation of AgNPs synthesized usingC.hirsuta(CAgNPs). The functional groups responsible for the reduction and stabilization of the NPs were identified using Fourier Transform Infrared (FTIR). The morphology, size, and elemental composition of the NPs were obtained using scanning electron microscope (SEM), transmission electron microscope (TEM), and energy dispersive X-ray spectroscopy (EDX). X-ray diffractometer was used to identify the phases and crystallinity of CAgNPs. Crystalline spherical NPs with average diameter of 6.9 ± 0.1 nm were successfully synthesized. The thermal analysis of CAgNPs was observed from DSC-TGA. The larvicidal results against the different larva instar stage ofCulex quinquefasciatusgave LC50= 4.43 ppm and LC90= 8.37 ppm. This is the first study on the synthesis of AgNPs usingC.hirsutaand its application against lymphatic filariasis vector. Hence, it is suggested that theC.hirsutasynthesized AgNPs would be environmentally benign in biological control of mosquito.

Fabrication and Characterization of Cellulose Acetate Ultrafine Fiber Containing Silver Nanoparticles by Electrospinning

2011 ◽  
Vol 337 ◽  
pp. 116-119 ◽  
Author(s):  
Dong Mei Zhao ◽  
Qing Mao Feng ◽  
Li Li Lv ◽  
Jian Li
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Cellulose Acetate ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Water Soluble ◽  
Ag Nps ◽  
Ultrafine Fibers ◽  
Transmission Electron ◽  
Electrospinning Method

Silver nanoparticles (Ag NPs)/cellulose acetate (CA) composite ultrafine fibers were successfully prepared by the electrospinning method. Water-soluble Ag NPs were directly mixed into CA polymer fibers to form organic–inorganic composite ultrafine fibers. The optical property of Ag NPs was measured by ultraviolet-visble spectrometer (UV-vis). The presence and identification of crystalline of Ag NPs were confirmed by XRD analysis. Transmission electron microscopy (TEM) images showed that silver nanoparticles (Ag NPs) with an average diameter of 5–15 nm were obtained and were well distributed in the CA ultrafine fibers. The morphologies of the as-prepared electrospun Ag NPs/CA composite ultrafine fibers were characterized by scanning electron microscopy (SEM) and TEM. The composition of fibers was characterized by FTIR spectrometer.

scholarly journals Preparation of silver nanoparticles by gamma Irradiation method using chitosan as stabilizer

2014 ◽  
Vol 4 (3) ◽  
pp. 43-46
Author(s):  
Tan Man Nguyen ◽  
Hai Le ◽  
Huu Tu Le ◽  
Thu Hong Tran ◽  
Duy Hang Nguyen ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Storage Time ◽  
Average Diameter ◽  
Colloidal Silver ◽  
Surface Plasma ◽  
Surface Plasma Resonance ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Γ Ray

Silver nanoparticles were prepared from (Ag+) aqueous solution by the method of γ-ray irradiation using chitosan as stabilizer. The saturated conversion dose (Ag+ à Ago) determined by UV-Vis spectroscopy was found to be about 16 kGy. The UV-Vis spectrum showed that an absorption peak at λmax = 400 nm due to surface plasma resonance. The image of transmission electron microscopy (TEM) showed that the silver nanoparticles were mostly spherical in shape and the average diameter was of about ~ 12 nm. The prepared colloidal silver nanoparticles solution was in good stability during storage time.

scholarly journals Biosynthesis of Anisotropic Silver Nanoparticles byBhargavaea indicaand Their Synergistic Effect with Antibiotics against Pathogenic Microorganisms

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Priyanka Singh ◽  
Yeon Ju Kim ◽  
Hina Singh ◽  
Ramya Mathiyalagan ◽  
Chao Wang ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Vibrio Parahaemolyticus ◽  
Size Range ◽  
Average Diameter ◽  
Pathogenic Microorganisms ◽  
X Ray Diffraction ◽  
X Ray ◽  
Extracellular Biosynthesis ◽  
Predominant Component ◽  
Transmission Electron

The strainBhargavaea indicaDC1 isolated from four-year-oldP. ginsengrhizospheric soil was used to perform rapid and extracellular biosynthesis of anisotropic silver nanoparticles. The ultraviolet-visible (UV-vis) spectra of the reaction mixture containing silver nanoparticles showed a peak at 460 nm, corresponding to the surface plasmon absorbance of silver nanoparticles. Field-emission transmission electron microscopy (FE-TEM) structural characterization revealed the nanobar, pentagon, spherical, icosahedron, hexagonal, truncated triangle, and triangular nanoparticles, with the size range from 30 to 100 nm. The energy-dispersive X-ray (EDX) analysis and elemental mapping results also confirmed that the silver was the predominant component of isolated nanoparticles. The X-ray diffraction (XRD) results correspond to the purity of silver nanoparticles and dynamic light scattering (DLS) result indicated that the average diameter of particles was 111.6 nm. In addition, enhancement in antimicrobial activity of commercial antibiotics was observed against various pathogenic microorganisms such asVibrio parahaemolyticus, Salmonella enterica, Staphylococcus aureus, Bacillus anthracis, Bacillus cereus, Escherichia coli, andCandida albicans.

scholarly journals Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1042 ◽  
Author(s):  
Sharad Bhatnagar ◽  
Toshiro Kobori ◽  
Deepak Ganesh ◽  
Kazuyoshi Ogawa ◽  
Hideki Aoyagi
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Positive Control ◽  
Significant Activity ◽  
Hek 293 ◽  
Transmission Electron ◽  
Elemental Silver ◽  
Human Liver Cancer ◽  
Biosynthesized Agnps ◽  
Extracellular Pigment

In recent years, green syntheses have been researched comprehensively to develop inexpensive and eco-friendly approaches for the generation of nanoparticles. In this context, plant and microbial sources are being examined to discover potential reducing agents. This study aims to utilize an extracellular pigment produced by Talaromyces purpurogenus as a prospective reducing agent to synthesize silver nanoparticles (AgNPs). Biosynthesized AgNPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), electron probe micro analyser (EPMA), and zeta potential. The pigment functional groups involved in the generation of AgNPs were investigated using Fourier transform infrared spectroscopy. TEM images showed that the generated nanoparticles were spherical, hexagonal, rod-shaped, and triangular-shaped with a particle size distribution from 4 to 41 nm and exhibited a surface plasmon resonance at around 410 nm. DLS and zeta potential studies revealed that the particles were polydispersed and stable (−24.8 mV). EPMA confirmed the presence of elemental silver in the samples. Biosynthesized AgNPs exhibited minimum inhibitory concentrations of 32 and 4 μg/mL against E. coli and S. epidermidis, respectively. Further, cytotoxicity of the AgNPs was investigated against human cervical cancer (HeLa), human liver cancer (HepG2), and human embryonic kidney (HEK-293) cell lines using 5-fluorouracil as a positive control. A significant activity was recorded against HepG2 cell line with a half-maximal inhibitory concentration of 11.1 μg/mL.

Hydrazine Based Facile Synthesis and Ordered Assembly of Metal Nanoparticles (Au, Ag) on a Bacterial Surface Layer Protein Template

2008 ◽  
Vol 8 (7) ◽  
pp. 3565-3569 ◽  
Author(s):  
Sarang S. Puranik ◽  
Hrushikesh M. Joshi ◽  
S. B. Ogale ◽  
K. M. Paknikar
Keyword(s):  
Silver Nanoparticles ◽  
Metal Nanoparticles ◽  
Metal Salt ◽  
Chemical Reduction ◽  
Average Diameter ◽  
Protein Array ◽  
Gold And Silver Nanoparticles ◽  
Bacterial Surface ◽  
Transmission Electron ◽  
Ordered Assembly

An efficient and facile procedure is developed for concurrent in situ synthesis and ordered assembly of metal nanoparticles on a periodic two dimensional protein array. The S-layer protein of Bacillus subtilis exhibiting uniform pore size is used as template. Synthesis of gold and silver nanoparticles anchoring on the pores of S-layer is achieved by chemical reduction of respective metal salt laden protein template. Transmission electron microscopy reveals formation of well ordered and separated gold and silver nanoparticles with an average diameter of 6 ± 1 nm and 4 ± 1 nm, respectively. The periodic arrangement of nanoparticles is dictated by the native structure of S-layer protein array as the nanoparticle locations are found to be correlated to the nanosized pores of the crystalline S-layer array.

In situ formation of silver nanoparticles based on dendriticlinear amphiphilic block copolymer micellar templates

e-Polymers ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Huiqing Wang ◽  
Ziqiang Shao ◽  
Wenjun Wang ◽  
Feijun Wang
Keyword(s):  
Aqueous Solution ◽  
Silver Nanoparticles ◽  
Block Copolymer ◽  
Average Diameter ◽  
Amphiphilic Block Copolymer ◽  
Uv Spectrum ◽  
H Nmr ◽  
Transmission Electron ◽  
Convergent Method ◽  
Absorbance Peak

AbstractDendritic-linear amphiphilic block copolymer, G1-b-PEG, was synthesized through a convergent method. The first generation benzylic dendritic polyether, G1-Cl, was synthesized, and benzylic dendritic polyether, G1-Cl, was used to react with linear monohydroxyl terminated MPEG in alkaline solution via a convergent method. The copolymer was characterized by 1H-NMR. The amphiphilic block copolymer could self-assemble into micelles in aqueous solution, which were used as templates to prepare dispersed silver nanoparticles in water. The morphology and structure of the hybrids were characterized by transmission electron microscopy (TEM) and ultraviolet spectroscopy (UV), 1H-NMR. An absorbance peak at 406 nm appeared after reduction in the UV spectrum. The average diameter of G1-B-PEG /Ag hybrid micelles was 186 nm, and the sliver nanoparticles are stable in aqueous solution due to the stabilization of the dendritic-linear micelles.

scholarly journals Ecofriendly/Rapid Synthesis of Silver Nanoparticles Using Extract of Waste Parts of Artichoke (Cynara scolymus L.) and Evaluation of their Cytotoxic and Antibacterial Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Ayşe Baran ◽  
Mehmet Fırat Baran ◽  
Cumali Keskin ◽  
Sevgi Irtegun Kandemir ◽  
Mahbuba Valiyeva ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Cell Lines ◽  
Analysis Data ◽  
Antibacterial Activities ◽  
Human Consumption ◽  
Cynara Scolymus ◽  
Microdilution Method ◽  
Mtt Method ◽  
Transmission Electron

Recycling wastes and providing their use in useful fields attract attention every day. In our study, with the extract prepared from the parts of the Cynara scolymus L. (artichoke) plant that is not suitable for human consumption, silver nanoparticles were easily synthesized in an ec-friendly, energy-free way. Characterization of the obtained nanoparticles was done with a UV-visible spectrophotometer (UV-Vis.), fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), and zeta potential analysis data. In these data, it was determined that AgNPs have a maximum absorbance at 458.8 nm wavelength, a crystal nanosize of 28.78 nm, and a spherical appearance. The zeta potential of (-) 16.9 mV indicates that silver nanoparticles exhibit a stable structure. Particles show antimicrobial effects on pathogenic species at concentrations of 0.03-0.25 μg/ml, and it was determined by using the minimum inhibition concentration (MIC) microdilution method. By examining their cytotoxic effects on U118, CaCo-2, and Skov-3 cancer cell lines and healthy HDF cell lines by the MTT method, concentrations of inhibitive effects on survival were determined.

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