Nicotinamide Mononucleotide Restores the Meiotic Competency of Porcine Oocytes Exposed to Ethylene Glycol Butyl Ether

Ethylene glycol butyl ether (EGBE), a type of glycol ethers, is a common chemical used in both industrial and household products. Increasing animal studies have indicated that it produces reproductive problems, such as testicular damage, reduced female fertility, death of embryos, and birth defects. However, how it influences the female germ cells has not yet determined. Here, we found that EGBE exposure resulted in the defective porcine oocyte maturation via disruption of cytoskeleton dynamics, showing the abnormal spindle assembly, chromosome alignment, and actin organization. Meanwhile, EGBE exposure perturbed the mitochondrial distribution and function, leading to the accumulation of reactive oxygen species (ROS) and generation of DNA damage and apoptosis. Of note, nicotinamide mononucleotide (NMN) supplementation rescued the meiotic defects caused by EGBE exposure via restoring NAD+ level and mitochondrial function and thus eliminating the excessive ROS. Taken together, our observations illustrate that NMN supplementation is an effective strategy to protect oocyte quality against environmental pollutant-induced deterioration, contributing to improve the animal and human fertility.

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Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.678665 ◽

2021 ◽

Vol 9 ◽

Author(s):

Jingyue Chen ◽

Zhaokang Cui ◽

Yawei Qiu ◽

Xingxing Zhang ◽

Fang Chen ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Dna Damage ◽

Reactive Oxygen ◽

Oocyte Quality ◽

Actin Dynamics ◽

Oxygen Species ◽

Cortical Granules ◽

Porcine Oocyte ◽

Mitochondrial Distribution ◽

And Function

Copper (Cu) is an essential trace element for animals, and also an important nutritional component for the normal physiology and metabolism of animal reproductive systems. An excess or lack of Cu will directly or indirectly affect animal reproductive activities. However, the effect of Cu, in particular excessive Cu, on the reproductive performance of sows has not been studied. Here, we report that excessive Cu had negative effects on oocyte maturation and organelle functions. We showed that Cu exposure perturbed porcine oocyte meiotic maturation and impaired spindle/chromosome structure, resulting in a defective spindle assembly, as well as the abnormal distribution of actin dynamics and cortical granules. In addition, single-cell transcriptome analysis identified the target effectors of Cu actions in porcine oocytes, further demonstrating that Cu exposure affects the mitochondrial distribution and function, leading to the high levels of reactive oxygen species, DNA damage, and early apoptosis of porcine oocytes. These findings demonstrate that Cu exposure causes abnormalities in the mitochondrial distribution and function, resulting in the increased oxidative stress and levels of reactive oxygen species, DNA damage, and apoptosis, ultimately leading to a decreased porcine oocyte quality.

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Clinical Toxicology of Ethylene Glycol Monoal Ethers

Human & Experimental Toxicology ◽

10.1177/096032719401300508 ◽

1994 ◽

Vol 13 (5) ◽

pp. 325-335 ◽

Cited By ~ 21

Author(s):

Randall G. Browning ◽

Steven C. Curry

Keyword(s):

Ethylene Glycol ◽

Animal Studies ◽

Case Reports ◽

Case Series ◽

Epidemiological Studies ◽

Acute Poisoning ◽

Clinical Toxicology ◽

Glycol Ethers ◽

Management Options ◽

Household Products

The glycol ethers constitute a family of organic solvents commonly found in industrial and household products. Because of their widespread availability and potential for serious toxicity, physicians should be aware of the clinical toxicology of these compounds. Until recently, knowledge of the toxic effects of glycol ethers has been derived from animal studies and a limited number of case reports and small case series. A growing body of data from epidemiological studies, controlled human studies, and studies using human tissue now allows for advancement in the understanding of the acute and chronic toxicity of these compounds. This review summarizes and evaluates human and pertinent animal literature on the clinical toxicology of glycol ethers, with a focus on the commonly encountered monoalkyl ethers of ethylene glycol. Management options for acute poisoning, as well as measures for the control of workplace exposures, are discussed.

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Ethylene glycol butyl ether deteriorates oocyte quality via impairing mitochondrial function

The FASEB Journal ◽

10.1096/fj.202002157r ◽

2021 ◽

Vol 35 (4) ◽

Author(s):

Xiaoyan Shi ◽

Yilong Miao ◽

Kemei Zhang ◽

Shuai Gong ◽

Bo Xiong

Keyword(s):

Ethylene Glycol ◽

Mitochondrial Function ◽

Oocyte Quality ◽

Butyl Ether

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A novel alkali and cosolvent thickening mechanism for latex

New Journal of Chemistry ◽

10.1039/c5nj01945a ◽

2015 ◽

Vol 39 (11) ◽

pp. 8984-8992 ◽

Cited By ~ 1

Author(s):

Lang He ◽

Yue Yu ◽

Zhengwei Cai ◽

Di Wang ◽

Xinlin Hong

Keyword(s):

Ethylene Glycol ◽

Butyl Ether ◽

Acrylic Latex

In this study, alkali-swellable acrylic latex (resin) and coalescent ethylene glycol butyl ether were combined to thicken the coating without the use of any thickeners.

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Ethylene Glycol Mono-n-Butyl Ether

Encyclopedia of Toxicology ◽

10.1016/b978-0-12-386454-3.00382-1 ◽

2014 ◽

pp. 528-531

Author(s):

B. Strohm

Keyword(s):

Ethylene Glycol ◽

Butyl Ether

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Effects of ethylene glycol ethers on the reproduction of Ceriodaphnia dubia

Chemosphere ◽

10.1016/s0045-6535(02)00400-9 ◽

2003 ◽

Vol 50 (3) ◽

pp. 373-376 ◽

Cited By ~ 2

Author(s):

J Devillers ◽

A Chezeau ◽

V Poulsen ◽

E Thybaud

Keyword(s):

Ethylene Glycol ◽

Ceriodaphnia Dubia ◽

Glycol Ethers ◽

Ethylene Glycol Ethers

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Longitudinal structural and functional brain development in childhood and adolescence

10.31234/osf.io/87kft ◽

2018 ◽

Author(s):

Kathryn L. Mills ◽

Christian K. Tamnes

Keyword(s):

Brain Development ◽

Animal Studies ◽

Diffusion Tensor ◽

Childhood And Adolescence ◽

Structural Development ◽

Social Environments ◽

Brain Structure And Function ◽

Magnetic Resonance Imaging Mri ◽

Methodological Considerations ◽

And Function

The development of the human brain involves a prolonged course of maturation, enabling us to learn to navigate our complex social environments. Here, we give short introductions to post-mortem and animal studies on postnatal brain development and selected methodological considerations for longitudinal developmental neuroimaging. We then describe typical developmental changes in brain structure and function from childhood to adulthood. We focus on measurements derived from magnetic resonance imaging (MRI) and on longitudinal data. Specifically, we discuss brain structural development based on morphometry and diffusion tensor imaging (DTI) studies, and functional development based on resting-state and task-based functional MRI. Finally, we highlight selected current overarching research questions and argue that an important step in answering these questions is to study individual differences in longitudinal brain development.

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HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.715093 ◽

2021 ◽

Vol 9 ◽

Author(s):

Emmi Helle ◽

Minna Ampuja ◽

Alexandra Dainis ◽

Laura Antola ◽

Elina Temmes ◽

...

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Animal Studies ◽

Cardiac Development ◽

Cell Types ◽

Human Endothelial Cells ◽

Bidirectional Communication ◽

Induced Pluripotent ◽

And Function ◽

Cell Crosstalk

Cell-cell interactions are crucial for organ development and function. In the heart, endothelial cells engage in bidirectional communication with cardiomyocytes regulating cardiac development and growth. We aimed to elucidate the organotypic development of cardiac endothelial cells and cardiomyocyte and endothelial cell crosstalk using human induced pluripotent stem cells (hiPSC). Single-cell RNA sequencing was performed with hiPSC-derived cardiomyocytes (hiPS-CMs) and endothelial cells (hiPS-ECs) in mono- and co-culture. The presence of hiPS-CMs led to increased expression of transcripts related to vascular development and maturation, cardiac development, as well as cardiac endothelial cell and endocardium-specific genes in hiPS-ECs. Interestingly, co-culture induced the expression of cardiomyocyte myofibrillar genes and MYL7 and MYL4 protein expression was detected in hiPS-ECs. Major regulators of BMP- and Notch-signaling pathways were induced in both cell types in co-culture. These results reflect the findings from animal studies and extend them to human endothelial cells, demonstrating the importance of EC-CM interactions during development.

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