scholarly journals Aqueous Triple-Phase System in Microwell Array for Generating Uniform-Sized DNA Hydrogel Particles

2021 ◽  
Vol 12 ◽  
Author(s):  
Marcos Kunihiro Masukawa ◽  
Yukiko Okuda ◽  
Masahiro Takinoue
Keyword(s):  
Physicochemical Characterization ◽  
Phase System ◽  
Two Phase ◽  
Dna Motifs ◽  
Hydrogel Microparticles ◽  
Hydrogel Particles ◽  
Thermal Gelation ◽  
Microwell Array ◽  
Phase Systems ◽  
Dna Hydrogel

DNA hydrogels are notable for their biocompatibility and ability to incorporate DNA information and computing properties into self-assembled micrometric structures. These hydrogels are assembled by the thermal gelation of DNA motifs, a process which requires a high salt concentration and yields polydisperse hydrogel particles, thereby limiting their application and physicochemical characterization. In this study, we demonstrate that single, uniform DNA hydrogel particles can form inside aqueous/aqueous two-phase systems (ATPSs) assembled in a microwell array. In this process, uniform dextran droplets are formed in a microwell array inside a microfluidic device. The dextran droplets, which contain DNA motifs, are isolated from each other by an immiscible PEG solution containing magnesium ions and spermine, which enables the DNA hydrogel to undergo gelation. Upon thermal annealing of the device, we observed the formation of an aqueous triple-phase system in which uniform DNA hydrogel particles (the innermost aqueous phase) resided at the interface of the aqueous two-phase system of dextran and PEG. We expect ATPS microdroplet arrays to be used to manufacture other hydrogel microparticles and DNA/dextran/PEG aqueous triple-phase systems to serve as a highly parallel model for artificial cells and membraneless organelles.

Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures

Lab on a Chip ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 2437-2444 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Steven G. Jones ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Interfacial Tension ◽  
Phase System ◽  
Flow Focusing ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microfluidic Flow ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ultralow Interfacial Tension

Simple microfluidic flow focusing generation of droplets from ultralow interfacial tension aqueous two phase systems (ATPS).

Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System

2011 ◽  
Vol 138-139 ◽  
pp. 995-1001 ◽  
Author(s):  
Wei Jie Wang ◽  
Xiao Le Zhang ◽  
Chang Bo Xu ◽  
Hong Yan Cheng
Keyword(s):  
High Purity ◽  
Separation Process ◽  
Phase System ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Optimized Conditions ◽  
Potassium Sodium Tartrate ◽  
Partitioning Behavior

In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

scholarly journals Tiny Bubbles

2009 ◽  
Vol 17 (1) ◽  
pp. 3-5
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Lower Energy ◽  
Critical Role ◽  
Interfacial Area ◽  
Consumer Products ◽  
Phase System ◽  
Two Phase ◽  
Liquid Phases ◽  
Phase Systems ◽  
Two Phases

This is not an article about the song made famous by the late (great) Don Ho. This is about a breakthrough in the understanding of how micrometer-sized bubbles can be stabilized for long periods of time. This can influence the taste, smell, and consistency of consumer products including food and cosmetics.In two-phase systems, which can include air (as bubbles) suspended within a liquid, the structures of the dispersed (bubbles) and continuous (liquid) phases play a critical role in determining the properties of the material. There is also the function of time in that the microstructure of the dispersed phase continuously evolves toward a state of lower energy by minimizing the surface area between the two phases (referred to as the interfacial area). In the long term, this time evolution diminishes the usefulness of two-phase systems. Emilie Dressaire, Rodney Bee, David Bell, Alex Lips, and Howard Stone have devised a way to stabilize a two-phase system for time periods of a year or longer.

Gas Column/Mass Spectrometry Analysis of Bio Oil Treated by Aqueous Two Phase System

2011 ◽  
Vol 236-238 ◽  
pp. 54-58
Author(s):  
Shao Peng Liu ◽  
Zhi Min Zong ◽  
Shan Shan Zhang ◽  
Yue Xu ◽  
Gui Zhen Gong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Spectrometry Analysis ◽  
Bio Oil ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ethanol System

Bio oil aqueous two phase systems were formed with ethanol and the salts of (NH4)2SO4, K2HPO4 and NaH2PO4 in bio oil on the ratio of 10:5:10(by mass). After phase separation, both the upper and the lower layers were analyzed by gas column/mass spectrometry (GC/MS). As a result, both K2HPO4/ethanol and NaH2PO4/ethanol system can enrich phenols and levoglucosan in the upperand lower layers,respectly, while NaH2PO4/ethanol system seldom changes the components of bio oil; the (NH4)2SO4/ethanol system is quit different with the other two and we have detected some components with nitrogen. Hence, NaH2PO4/ethanol is an advantage aqueous two phase system for bio oil treatment.

scholarly journals Extraction of Fe (III) and Zn (II) by ionic liquids in a system based on polypropylene glycol 425 and sodium chloride.

2020 ◽  
Vol 11 (3-2020) ◽  
pp. 72-75
Author(s):  
I. V. Zinov’eva ◽  
◽  
M. I. Fedorova ◽  
Yu. A. Zakhodyaeva ◽  
A.A. Voshkin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Ionic Liquids ◽  
Sodium Chloride ◽  
Phase System ◽  
Polypropylene Glycol ◽  
Two Phase ◽  
Study Results ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Extraction Of Metals

This work presents the experimental study results of Fe(III) and Zn(II) extraction by ionic liquids based on trioctylmethylammonium chloride and rhodanide in an aqueous two-phase system based on polypropylene glycol 425 and sodium chloride. Based on the results obtained, it was concluded that these extractants are effective for the extraction of metals from aqueous solutions, and aqueous two-phase systems represent a “green” alternative to traditional extraction systems.

scholarly journals Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

2021 ◽  
Author(s):  
Sohyung Lee ◽  
Joe de Rutte ◽  
Robert Dimatteo ◽  
Doyeon Koo ◽  
Dino Di Carlo
Keyword(s):  
Phase Separation ◽  
Phase System ◽  
Cell Secretion ◽  
Two Phase ◽  
Hollow Shell ◽  
Hydrogel Microparticles ◽  
Trade Offs ◽  
Shell Particles ◽  
Scalable Production ◽  
Formation Phase

Microparticles with defined shapes and spatial chemical modification can enable new opportunities to interface with cells and tissues at the cellular scale. However, conventional methods to fabricate shaped microparticles have trade-offs between the throughput of manufacture and precision of particle shape and chemical functionalization. Here, we achieved scalable production of hydrogel microparticles at rates of greater than 40 million/hour with localized surface chemistry using a parallelized step emulsification device and temperature-induced phase-separation. The approach harnesses a polymerizable polyethylene glycol (PEG) and gelatin aqueous-two phase system (ATPS) which conditionally phase separates within microfluidically-generated droplets. Following droplet formation, phase separation is induced and phase separated droplets are subsequently crosslinked to form uniform crescent and hollow shell particles with gelatin functionalization on the boundary of the cavity. The gelatin localization enabled deterministic cell loading in nanoliter-sized crescent-shaped particles, which we refer to as nanovials, with cavity dimensions tuned to the size of cells. Loading on nanovials also imparted improved cell viability during analysis and sorting using standard fluorescence activated cell sorters, presumably by protecting cells from shear stress. This localization effect was further exploited to selectively functionalize capture antibodies to nanovial cavities enabling single-cell secretion assays with reduced cross-talk in a simplified format.

Treatment of Oily Cafeteria Wastewater by Single-Phase and Two-Phase Anaerobic Filter

1990 ◽  
Vol 22 (3-4) ◽  
pp. 299-306 ◽  
Author(s):  
Keisuke Hanaki ◽  
Tomonori Matsuo ◽  
Katsuo Kumazaki
Keyword(s):  
Single Phase ◽  
Material Balance ◽  
Phase System ◽  
Two Phase ◽  
Term Operation ◽  
Effluent Quality ◽  
Anaerobic Filter ◽  
Phase Systems ◽  
Better Than

Cafeteria wastewater containing about 30% of lipid on COD basis was treated at 20°C by laboratory-scale anaerobic filter in single-phase system and two-phase systems. Stable COD removal (about 80%) was achieved in spite of large fluctuation in influent COD concentration (normal range of 1,300 - 2,500 mg−l) both in the single-phase system (hydraulic retention time (HRT) longer than 1.3 d) and in the two-phase system (HRT longer than 3.3 d). The single-phase system gave better effluent quality than the two-phase system because the former entrapped suspended solids better than the latter. However, material balance revealed that methane conversion from removed COD was higher in the two-phase system than the single-phase system. The single-phase system perhaps removed lipids by entrapment with filter media without biodegradation, and this might cause clogging problems in long-term operation. The two-phase system is recommended since it degrades lipids better than the single-phase system.

Passive droplet generation in aqueous two-phase systems with a variable-width microchannel

Soft Matter ◽  
2019 ◽  
Vol 15 (23) ◽  
pp. 4647-4655 ◽  
Author(s):  
Daeho Choi ◽  
Eunjeong Lee ◽  
Sung-Jin Kim ◽  
Minsub Han
Keyword(s):  
Droplet Generation ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Passive droplet generation for an aqueous two-phase system (ATPS) was performed with a fracture-based variable microchannel.

scholarly journals Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Oğuz Kaan Kırbaş ◽  
Batuhan Turhan Bozkurt ◽  
Ayla Burçin Asutay ◽  
Beyza Mat ◽  
Bihter Ozdemir ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Cost Effective ◽  
Drug Carriers ◽  
Phase System ◽  
Phenol Red ◽  
Two Phase ◽  
Acid Acid ◽  
Aqueous Two Phase Systems ◽  
Specialized Equipment ◽  
Phase Systems

AbstractFrom biomarkers to drug carriers, Extracellular Vesicles (EVs) are being used successfully in numerous applications. However, while the subject has been steadily rising in popularity, current methods of isolating EVs are lagging behind, incapable of isolating EVs at a high enough quantity or quality while also requiring expensive, specialized equipment. The “isolation problem” is one of the major obstacles in the field of EV research - and even more so for their potential, widespread use for clinical diagnosis and therapeutic applications. Aqueous Two-Phase Systems (ATPS) has been reported previously as a promising method for isolating EVs quickly and efficiently, and with little contaminants - however, this method has not seen widespread use. In this study, an ATPS-based isolation protocol is used to isolate small EVs from plant, cell culture, and parasite culture sources. Isolated EVs were characterized in surface markers, size, and morphological manner. Additionally, the capacity of ATPS-based EV isolation in removing different contaminants was shown by measuring protein, fatty acid, acid, and phenol red levels of the final isolate. In conclusion, we have shown that EVs originating from different biological sources can be isolated successfully in a cost-effective and user-friendly manner with the use of aqueous two-phase systems.

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