Monofloral Triadica Cochinchinensis Honey Polyphenols Improve Alcohol-Induced Liver Disease by Regulating the Gut Microbiota of Mice

Honey produced from medicinal plants holds great promise for human health. Increasing evidence suggests that the gut microbiota plays an important role in liver pathology after alcohol intake. The aim of this study was to identify the polyphenol composition of triadica cochinchinensis honey (TCH), and to study the potential effect of honey polyphenols on the regulation of gut microbes in mice with alcohol-induced liver injury and the improvement of alcohol-induced liver disease. For these purposes, a total of 190 compounds were identified and 27 of them were quantified by ultraperformance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q/TOF-MS) and we successfully established a mouse model of alcohol-induced liver injury. The results show that TCH polyphenols can significantly restore the levels of ALT and AST, and TCH intervention can significantly improve the pathological changes of liver tissue in alcohol-exposed mice. Additionally, a significant decrease was observed in Firmicutes/Bacteroidetes after TCH treatment. Moreover, KEGG pathways of ATP-binding cassette (ABC) transporters, two-component system and biosynthesis of amino acids enriched the most differentially expressed genes after TCH intervention for 8 weeks. Our results may have important implications for the use of TCH as a functional food component with potential therapeutic utility against alcohol-induced liver disease.

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Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake

Journal of Functional Foods ◽

10.1016/j.jff.2020.104045 ◽

2020 ◽

Vol 72 ◽

pp. 104045

Author(s):

Xiaozhu Tian ◽

Rong Li ◽

Yiming Jiang ◽

Fei Zhao ◽

Zhengsheng Yu ◽

...

Keyword(s):

Liver Disease ◽

Gut Microbiota ◽

Alcoholic Liver Disease ◽

Alcohol Intake ◽

Chronic Alcohol ◽

Bifidobacterium Breve

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The Protective Effects of Live and Pasteurized Akkermansia muciniphila and Its Extracellular Vesicles against HFD/CCl4-Induced Liver Injury

Microbiology Spectrum ◽

10.1128/spectrum.00484-21 ◽

2021 ◽

Author(s):

Shahrbanoo Keshavarz Azizi Raftar ◽

Fatemeh Ashrafian ◽

Abbas Yadegar ◽

Arezou Lari ◽

Hamid Reza Moradi ◽

...

Keyword(s):

Liver Disease ◽

Liver Fibrosis ◽

Liver Injury ◽

Gut Microbiota ◽

Chronic Liver Disease ◽

Liver Dysfunction ◽

Protective Effects ◽

Main Determinant ◽

Content Type ◽

Akkermansia Muciniphila

Akkermansia muciniphila , as a member of the gut microbiota, has been proposed as a next-generation probiotic. Liver fibrosis is the main determinant of liver dysfunction and mortality in patients with chronic liver disease.

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Streptococcus, the Predominant Bacterium to Predict the Severity of Liver Injury in Alcoholic Liver Disease

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.649060 ◽

2021 ◽

Vol 11 ◽

Author(s):

Xiaodan Zhong ◽

Ping Cui ◽

Junjun Jiang ◽

Chuanyi Ning ◽

Bingyu Liang ◽

...

Keyword(s):

Liver Disease ◽

Liver Injury ◽

Gut Microbiota ◽

Alcoholic Liver Disease ◽

Early Stage ◽

Characteristic Curve ◽

Hepatic Function ◽

Rrna Gene ◽

Alcoholic Fatty Liver ◽

Predominant Bacterium

BackgroundNew evidence implies that the imbalance of gut microbiota is associated with the progression of alcoholic liver disease (ALD) and that the composition of gut microbiota is altered in ALD patients. However, the predominant bacterium in patients involved in the progress of ALD has not been identified. The purpose of this study is to investigate the predominant bacterium in the early and end-stages of ALD as well as the relationship between the bacterium and the degree of liver injury.MethodsWe enrolled 21 alcoholic fatty liver (AFL) patients, 17 alcoholic liver cirrhosis (ALC) patients and 27 healthy controls, and sequenced the 16S rRNA gene of their fecal microbiota. The gut microbiota composition and its relationship with the indicators of clinical hepatic function were assessed using canonical correspondence analysis (CCA), spearman correlation heatmap and multivariate association with linear (MaAsLin) Models.ResultsThe composition and structure of gut microbiota changed greatly in different stages of ALD, and the degree of disorder was aggravated with the progression of ALD, even in the early stage. Moreover, the relative abundance of Streptococcus was highly enriched only in patients with ALC (P <0.001), and positively correlated with AST level (P = 0.029). The abundance of Streptococcus distinguished the liver injury of ALC patients from the controls with an area under the receiver-operating characteristic curve (AUC) of 0.877 (P < 0.001).ConclusionsThese findings indicate that the imbalance of gut microbiota exists at the early and end-stages of ALD, and the degree of disorder is aggravated with the progression of ALD. Streptococcus, as the predominant bacterium, may be a microbiological marker to evaluate the severity of liver injury in ALD patients.

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Antrodin A from Antrodia camphorata modulates the gut microbiome and liver metabolome in mice exposed to acute alcohol intake

Food & Function ◽

10.1039/d0fo03345f ◽

2021 ◽

Vol 12 (7) ◽

pp. 2925-2937

Author(s):

Zhenwei Yi ◽

Xiaofeng Liu ◽

Lihong Liang ◽

Guangqiang Wang ◽

Zhiqiang Xiong ◽

...

Keyword(s):

Liver Injury ◽

Gut Microbiota ◽

Gut Microbiome ◽

Alcohol Intake ◽

Underlying Mechanism ◽

Antrodia Camphorata ◽

Hepatoprotective Effects

In this study, we investigated the hepatoprotective effects of AdA and the underlying mechanism at the liver metabolomics and gut microbiota levels under alcohol-induced liver injury conditions.

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Astaxanthin Prevents Alcoholic Fatty Liver Disease by Modulating Mouse Gut Microbiota

Nutrients ◽

10.3390/nu10091298 ◽

2018 ◽

Vol 10 (9) ◽

pp. 1298 ◽

Cited By ~ 18

Author(s):

Huilin Liu ◽

Meihong Liu ◽

Xueqi Fu ◽

Ziqi Zhang ◽

Lingyu Zhu ◽

...

Keyword(s):

Liver Disease ◽

Liver Injury ◽

Gut Microbiota ◽

Fatty Liver ◽

Fatty Liver Disease ◽

Serum Markers ◽

Alcoholic Fatty Liver ◽

Ethanol Group ◽

The Relationship ◽

Alcoholic Fatty Liver Disease

The development and progression of alcoholic fatty liver disease (AFLD) is influenced by the intestinal microbiota. Astaxanthin, a type of oxygenated carotenoid with strong antioxidant and anti-inflammatory properties, has been proven to relieve liver injury. However, the relationship between the gut microbiota regulation effect of astaxanthin and AFLD improvement remains unclear. The effects of astaxanthin on the AFLD phenotype, overall structure, and composition of gut microbiota were assessed in ethanol-fed C57BL/6J mice. The results showed that astaxanthin treatment significantly relieves inflammation and decreases excessive lipid accumulation and serum markers of liver injury. Furthermore, astaxanthin was shown to significantly decrease species from the phyla Bacteroidetes and Proteobacteria and the genera Butyricimonas, Bilophila, and Parabacteroides, as well as increase species from Verrucomicrobia and Akkermansia compared with the Et (ethanol)group. Thirteen phylotypes related to inflammation as well as correlated with metabolic parameters were significantly altered by ethanol, and then notably reversed by astaxanthin. Additionally, astaxanthin altered 18 and 128 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways involved in lipid metabolism and xenobiotic biodegradation and metabolism at levels 2 and 3, respectively. These findings suggest that Aakkermansia may be a potential target for the astaxanthin-induced alleviation of AFLD and may be a potential treatment for bacterial disorders induced by AFLD.

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The Prebiotic-Like Effects of Coprinus comatus Polysaccharides on Gut Microbiota in Normal Mice and Those with Acute Alcoholic Liver Injury: A Comparative Study

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/2027570 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Weidong Li ◽

Yongxia Wang ◽

Min Sun ◽

Yuting Liang ◽

Xiaoqing Cai ◽

...

Keyword(s):

Liver Injury ◽

Gut Microbiota ◽

Comparative Study ◽

Relative Abundance ◽

Alcohol Intake ◽

Alcoholic Liver Injury ◽

Alcohol Group ◽

Coprinus Comatus ◽

Positive Effects ◽

Microbiota Structure

This study aims to investigate the prebiotic-like effects of Coprinus comatus polysaccharides (CCP) on gut microbiota. Mice were divided into four groups: normal group (NG), alcohol group (AG), polysaccharides group (PG), and alcohol + polysaccharides group (APG). The gut microbiota structure of feces was analyzed by determining the V3-V4 region sequence in 16S rDNA. The results showed CCP could increase the diversity of gut microbiota. Compared with NG, PG had a significantly higher relative abundance of Firmicutes and Lactobacillaceae and a lower abundance of Rikenellaceae. These changes in gut microbiota result in positive effects on gut due to a series of prebiotic-like effects of CCP. At the same time, CCP could improve some adverse changes in gut microbiota caused by acute alcohol intake, such as the increased proportion of Firmicutes, Bacteroidetes, Muribaculaceae, and Lachnospiraceae and the decreased proportion of Rikenellaceae. In conclusion, the CCP has certain prebiotic effects not only on normal mice but also on mice with acute alcoholic liver injury.

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Effects of Tea against Alcoholic Fatty Liver Disease by Modulating Gut Microbiota in Chronic Alcohol-Exposed Mice

Foods ◽

10.3390/foods10061232 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1232

Author(s):

Bangyan Li ◽

Qianqian Mao ◽

Dandan Zhou ◽

Min Luo ◽

Renyou Gan ◽

...

Keyword(s):

Liver Disease ◽

Liver Injury ◽

Gut Microbiota ◽

Fatty Liver ◽

Fatty Liver Disease ◽

Black Tea ◽

Chronic Alcohol ◽

Alcoholic Fatty Liver ◽

Oolong Tea ◽

Alcoholic Fatty Liver Disease

Gut microbiota dysbiosis has been a crucial contributor to the pathogenesis of alcoholic fatty liver disease (AFLD). Tea is a popular beverage worldwide and exerts antioxidant and anti-inflammatory activities, as well as hepatoprotective effects. However, the potential role of gut microbiota regulated by tea in the prevention and management of AFLD remains unclear. Here, the protective effects of oolong tea, black tea, and dark tea on AFLD and its regulation of gut microbiota in chronic alcohol-exposed mice were explored and investigated. The results revealed that tea supplementation significantly prevented liver steatosis, decreased oxidative stress and inflammation, and modulated gut microbiota in chronic alcohol-exposed mice, especially oolong tea and dark tea. However, black tea showed less effectiveness against liver injury caused by alcohol. Moreover, the diversity, structure and composition of chronic alcohol-disrupted gut microbiota were restored by the supplementation of oolong tea and dark tea based on the analysis of gut microbiota. Furthermore, the relationship between liver injury biochemical indicators and gut microbiota indicated that some specific bacteria, such as Bacteroides, Alloprevotella, and Parabacteroides were closely associated with AFLD. In addition, the phytochemical components in tea extracts were measured by high-performance liquid chromatography, which could contribute to preventive effects on AFLD. In summary, oolong tea and dark tea could prevent chronic alcohol exposure-induced AFLD by modulating gut microbiota.

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