scholarly journals Comparative Genome-Wide Transcriptome Analysis of Brucella suis and Brucella microti Under Acid Stress at pH 4.5: Cold Shock Protein CspA and Dps Are Associated With Acid Resistance of B. microti

2021 ◽  
Vol 12 ◽  
Author(s):  
Jorge A. de la Garza-García ◽  
Safia Ouahrani-Bettache ◽  
Sébastien Lyonnais ◽  
Erika Ornelas-Eusebio ◽  
Luca Freddi ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Protein Misfolding ◽  
Cold Shock ◽  
Stress Protein ◽  
Acid Stress ◽  
Acid Resistance ◽  
Cold Shock Protein ◽  
Physiological Adaptation ◽  
Energy Conditions ◽  
Brucella Suis

Brucellae are facultative intracellular coccobacilli causing brucellosis, one of the most widespread bacterial zoonosis affecting wildlife animals, livestock and humans. The genus Brucella comprises classical and atypical species, such as Brucella suis and Brucella microti, respectively. The latter is characterized by increased metabolic activity, fast growth rates, and extreme acid resistance at pH 2.5, suggesting an advantage for environmental survival. In addition, B. microti is more acid-tolerant than B. suis at the intermediate pH of 4.5. This acid-resistant phenotype of B. microti may have major implications for fitness in soil, food products and macrophages. Our study focused on the identification and characterization of acid resistance determinants of B. suis and B. microti in Gerhardt’s minimal medium at pH 4.5 and 7.0 for 20 min and 2 h by comparative RNA-Seq-based transcriptome analysis, validated by RT-qPCR. Results yielded a common core response in both species with a total of 150 differentially expressed genes, and acidic pH-dependent genes regulated specifically in each species. The identified core response mechanisms comprise proton neutralization or extrusion from the cytosol, participating in maintaining physiological intracellular pH values. Differential expression of 441 genes revealed species-specific mechanisms in B. microti with rapid physiological adaptation to acid stress, anticipating potential damage to cellular components and critical energy conditions. Acid stress-induced genes encoding cold shock protein CspA, pseudogene in B. suis, and stress protein Dps were associated with survival of B. microti at pH 4.5. B. suis response with 284 specifically regulated genes suggested increased acid stress-mediated protein misfolding or damaging, triggering the set-up of repair strategies countering the consequences rather than the origin of acid stress and leading to subsequent loss of viability. In conclusion, our work supports the hypothesis that increased acid stress resistance of B. microti is based on selective pressure for the maintenance of functionality of critical genes, and on specific differential gene expression, resulting in rapid adaptation.

Effect of different carbon sources and cold shock on protein synthesis by a psychrotrophicAcinetobactersp.

2002 ◽  
Vol 48 (3) ◽  
pp. 239-244 ◽  
Author(s):  
S E Barbaro ◽  
J T Trevors ◽  
W E Inniss
Keyword(s):  
Protein Synthesis ◽  
Olive Oil ◽  
Cold Shock ◽  
Polyacrylamide Gel Electrophoresis ◽  
Carbon Sources ◽  
Stress Protein ◽  
Tween 80 ◽  
Synthesis Temperature ◽  
Cold Shock Protein ◽  
In Cells

The induction of proteins after a 25 to 5°C cold shock in the psychrotrophic Acinetobacter HH1-1 was examined using two-dimensional polyacrylamide gel electrophoresis. In addition, effects of various carbon sources (acetate, Tween 80, and olive oil) on protein synthesis after cold shock were assessed. HH1-1 responded to cold shock by synthesizing both cold shock proteins (csps) and cold acclimation proteins (caps). The synthesis of two csps (89 and 18) was increased 2 h after cold shock by the cells, regardless of the carbon source provided. An additional csp (csp 12), with an estimated molecular mass of 12 kDa, was observed in cells grown in olive oil only. Csp 12 was also synthesized when cells were incubated at 30°C, suggesting that this protein may serve as a general stress protein. In addition to csps, caps were observed post cold shock at 72 h in acetate-grown cells and at 140 h in cells grown in Tween 80 and olive oil. Induction of cold-acclimated periplasmic proteins was observed for cells grown in olive oil only, suggesting cells grown in olive oil may be stressed by low temperatures to a greater extent than cells grown in either acetate or Tween 80.Key words: Acinetobacter, carbon sources, cold shock, protein synthesis, temperature.

scholarly journals Contribution of YthA, a PspC Family Transcriptional Regulator ofLactococcus lactisF44 Acid Tolerance and Nisin Yield: a Transcriptomic Approach

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Hao Wu ◽  
Jingui Liu ◽  
Sen Miao ◽  
Yue Zhao ◽  
Hongji Zhu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Survival Rate ◽  
Transcriptome Analysis ◽  
Acid Stress ◽  
Acid Resistance ◽  
Transcriptional Regulator ◽  
Regulation Mechanism ◽  
Content Type ◽  
Regulation Mechanisms ◽  
Nisin Yield

ABSTRACTTo overcome the adverse impacts of environmental stresses during growth, different adaptive regulation mechanisms can be activated inLactococcus lactis. In this study, the transcription levels of eight transcriptional regulators ofL. lactissubsp.lactisF44 under acid stress were analyzed using quantitative reverse transcription-PCR. Eight gene-overexpressing strains were then constructed to examine their influences on acid-resistant capability. OverexpressingythA, a PspC family transcriptional regulator, increased the survival rate by 3.2-fold compared to the control at the lethal pH 3.0 acid shock. Moreover, the nisin yield was increased by 45.50%. TheythA-overexpressing strain FythA appeared to have higher intracellular pH stability and nisin-resistant ability. Subsequently, transcriptome analysis revealed that the vast majority of genes associated with amino acid biosynthesis, including arginine, serine, phenylalanine, and tyrosine, were predominantly upregulated in FythA. Arginine biosynthesis (argGandargH), arginine deiminase pathway, and polar amino acid transport (ysfEandysfF) were proposed to be the main regulation mechanisms of YthA. Furthermore, the transcription of genes associated with pyrimidine and exopolysaccharide biosynthesis were upregulated. The transcriptional levels ofnisIPRKFEGgenes were substantially higher in FythA, which directly contributed to the yield and resistance of nisin. Three potential DNA-binding sequences were predicted by computer analysis using the upstream regions of genes with prominent changes. This study showed that YthA could increase acid resistance and nisin yield and revealed a putative regulation mechanism of YthA.IMPORTANCENisin, produced byLactococcus lactissubsp.lactis, is widely used as a safe food preservative. Acid stress becomes the primary restrictive factor of cell growth and nisin yield. In this research, we found that the transcriptional regulator YthA was conducive to enhancing the acid resistance ofL. lactisF44. OverexpressingythAcould significantly improve the survival rate and nisin yield. The stability of intracellular pH and nisin resistance were also increased. Transcriptome analysis showed that nisin immunity and the biosynthesis of some amino acids, pyrimidine, and exopolysaccharides were enhanced in the engineered strain. This study elucidates the regulation mechanism of YthA and provides a novel strategy for constructing robust industrialL. lactisstrains.

Study on the transgenic tobacco expressing truncated wheat cold shock protein gene TA3-13 and analysis of disease resistance

2011 ◽  
Vol 33 (5) ◽  
pp. 520-526 ◽  
Author(s):  
Na LI ◽  
Xiu-Zhen DU ◽  
Xiao-Mei PAN ◽  
Jin-Sheng WANG ◽  
Cong-Feng SONG
Keyword(s):  
Disease Resistance ◽  
Transgenic Tobacco ◽  
Cold Shock ◽  
Protein Gene ◽  
Cold Shock Protein

Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis

2015 ◽  
Vol 112 ◽  
pp. 15-20 ◽  
Author(s):  
Antje Lindae ◽  
Raphael J. Eberle ◽  
Icaro P. Caruso ◽  
Monika A. Coronado ◽  
Fabio R. de Moraes ◽  
...  
Keyword(s):  
Cold Shock ◽  
Protein A ◽  
Cold Shock Protein ◽  
Corynebacterium Pseudotuberculosis ◽  
Purification And Characterization

Dynamical properties of cold shock protein A from Mycobacterium tuberculosis

2010 ◽  
Vol 402 (4) ◽  
pp. 693-698 ◽  
Author(s):  
Gabriella D’Auria ◽  
Carla Esposito ◽  
Lucia Falcigno ◽  
Luisa Calvanese ◽  
Emanuela Iaccarino ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cold Shock ◽  
Protein A ◽  
Cold Shock Protein ◽  
Dynamical Properties

scholarly journals Major cold shock protein of Escherichia coli.

1990 ◽  
Vol 87 (1) ◽  
pp. 283-287 ◽  
Author(s):  
J. Goldstein ◽  
N. S. Pollitt ◽  
M. Inouye
Keyword(s):  
Escherichia Coli ◽  
Cold Shock ◽  
Cold Shock Protein

scholarly journals Metabolomic Evaluation of Ralstonia solanacearum Cold Shock Protein Peptide (csp22)-Induced Responses in Solanum lycopersicum

2022 ◽  
Vol 12 ◽  
Author(s):  
Dylan R. Zeiss ◽  
Paul A. Steenkamp ◽  
Lizelle A. Piater ◽  
Ian A. Dubery
Keyword(s):  
Solanum Lycopersicum ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Plant Pathogens ◽  
Cold Shock ◽  
Phenylpropanoid Pathway ◽  
Infection Process ◽  
Cold Shock Protein ◽  
Pre Treatment ◽  
Molecular Patterns

Ralstonia solanacearum, the causal agent of bacterial wilt, is one of the most destructive bacterial plant pathogens. This is linked to its evolutionary adaptation to evade host surveillance during the infection process since many of the pathogen’s associated molecular patterns escape recognition. However, a 22-amino acid sequence of R. solanacearum-derived cold shock protein (csp22) was discovered to elicit an immune response in the Solanaceae. Using untargeted metabolomics, the effects of csp22-elicitation on the metabolome of Solanum lycopersicum leaves were investigated. Additionally, the study set out to discover trends that may suggest that csp22 inoculation bestows enhanced resistance on tomato against bacterial wilt. Results revealed the redirection of metabolism toward the phenylpropanoid pathway and sub-branches thereof. Compared to the host response with live bacteria, csp22 induced a subset of the discriminant metabolites, but also metabolites not induced in response to R. solanacearum. Here, a spectrum of hydroxycinnamic acids (especially ferulic acid), their conjugates and derivatives predominated as signatory biomarkers. From a metabolomics perspective, the results support claims that csp22 pre-treatment of tomato plants elicits increased resistance to R. solanacearum infection and contribute to knowledge on plant immune systems operation at an integrative level. The functional significance of these specialized compounds may thus support a heightened state of defense that can be applied to ward off attacking pathogens or toward priming of defense against future infections.

scholarly journals A Universal Stress Protein upregulated by hypoxia may contribute to chronic lung colonisation and intramacrophage survival in cystic fibrosis

2020 ◽  
Author(s):  
Andrew O’Connor ◽  
Rita Berisio ◽  
Mary Lucey ◽  
Kirsten Schaffer ◽  
Siobhán McClean
Keyword(s):  
Oxidative Stress ◽  
Chronic Infection ◽  
Stress Proteins ◽  
Cell Attachment ◽  
Stress Protein ◽  
Acid Stress ◽  
Nutrient Deficiency ◽  
Fold Increase ◽  
Burkholderia Cenocepacia ◽  
Low Oxygen

SummaryUniversal stress proteins (USPs) are ubiquitously expressed in bacteria, plants and eukaryotes and play a lead role in adaptation to environmental conditions. In Gram negative bacteria they enable adaption of bacterial pathogens to the conditions encountered in the human niche, including hypoxia, oxidative stress, osmotic stress, nutrient deficiency or acid stress, thereby facilitating colonisation. We previously reported that all six USP proteins encoded within a low-oxygen responsive locus in Burkholderia cenocepacia showed increased abundance during chronic colonisation of the CF lung. However, the role of USPs in chronic infection is not known. Using mutants derived from B. cenocepacia strain, K56-2, we show that USP76 is required for growth and survival in many conditions associated with the CF lung including, hypoxia, acidic conditions, oxidative stress. Moreover, it is involved in attachment to host epithelial cells, but not virulence. It also has a role in survival in macrophages isolated from people with CF. In contrast, another USP encoded in the same locus, USP92 had no effect on host cell attachment or oxidative stress, but was responsible for a 3-fold increase in virulence. Overall this shows that these USPs, both upregulated during chronic infection, have distinct roles in Burkholderia pathogenesis and may support the survival of B. cenocepacia in the CF lung. Specifically, USP76 is involved in its survival within CF macrophages, a hallmark of Burkholderia infection.

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