scholarly journals Prospective Use of Brown Spider Venom Toxins as Therapeutic and Biotechnological Inputs

2021 ◽  
Vol 8 ◽  
Author(s):  
Luiza Helena Gremski ◽  
Fernando Hitomi Matsubara ◽  
Nayanne Louise Costacurta Polli ◽  
Bruno Cesar Antunes ◽  
Pedro Henrique de Caires Schluga ◽  
...  
Keyword(s):  
Renal Injury ◽  
Therapeutic Agents ◽  
Biologically Active ◽  
Venom Toxins ◽  
Potential Development ◽  
Rational Therapy ◽  
Protein Toxins ◽  
Brown Spider ◽  
Hematological Abnormalities ◽  
New Generation

Brown spider (genus Loxosceles) venoms are mainly composed of protein toxins used for predation and defense. Bites of these spiders most commonly produce a local dermonecrotic lesion with gravitational spread, edema and hemorrhage, which together are defined as cutaneous loxoscelism. Systemic loxoscelism, such as hematological abnormalities and renal injury, are less frequent but more lethal. Some Loxosceles venom toxins have already been isolated and extensively studied, such as phospholipases D (PLDs), which have been recombinantly expressed and were proven to reproduce toxic activities associated to the whole venom. PLDs have a notable potential to be engineered and converted in non-toxic antigens to produce a new generation of antivenoms or vaccines. PLDs also can serve as tools to discover inhibitors to be used as therapeutic agents. Other Loxosceles toxins have been identified and functionally characterized, such as hyaluronidases, allergen factor, serpin, TCTP and knottins (ICK peptides). All these toxins were produced as recombinant molecules and are biologically active molecules that can be used as tools for the potential development of chemical candidates to tackle many medical and biological threats, acting, for instance, as antitumoral, insecticides, analgesic, antigens for allergy tests and biochemical reagents for cell studies. In addition, these recombinant toxins may be useful to develop a rational therapy for loxoscelism. This review summarizes the main candidates for the development of drugs and biotechnological inputs that have been described in Brown spider venoms.

scholarly journals Activity of Anti-Microbial Peptides (AMPs) against Leishmania and Other Parasites: An Overview

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 984
Author(s):  
Rima El-Dirany ◽  
Hawraa Shahrour ◽  
Zeinab Dirany ◽  
Fadi Abdel-Sater ◽  
Gustavo Gonzalez-Gaitano ◽  
...  
Keyword(s):  
Innate Immune System ◽  
Therapeutic Agents ◽  
Mechanisms Of Action ◽  
Innate Immune ◽  
Biologically Active ◽  
Neglected Tropical Disease ◽  
Parasitic Diseases ◽  
Promising Alternative ◽  
Parasitic Effect ◽  
Anti Fungal

Anti-microbial peptides (AMPs), small biologically active molecules, produced by different organisms through their innate immune system, have become a considerable subject of interest in the request of novel therapeutics. Most of these peptides are cationic-amphipathic, exhibiting two main mechanisms of action, direct lysis and by modulating the immunity. The most commonly reported activity of AMPs is their anti-bacterial effects, although other effects, such as anti-fungal, anti-viral, and anti-parasitic, as well as anti-tumor mechanisms of action have also been described. Their anti-parasitic effect against leishmaniasis has been studied. Leishmaniasis is a neglected tropical disease. Currently among parasitic diseases, it is the second most threating illness after malaria. Clinical treatments, mainly antimonial derivatives, are related to drug resistance and some undesirable effects. Therefore, the development of new therapeutic agents has become a priority, and AMPs constitute a promising alternative. In this work, we describe the principal families of AMPs (melittin, cecropin, cathelicidin, defensin, magainin, temporin, dermaseptin, eumenitin, and histatin) exhibiting a potential anti-leishmanial activity, as well as their effectiveness against other microorganisms.

Brown spider venom toxins interact with cell surface and are endocytosed by rabbit endothelial cells

Toxicon ◽  
2010 ◽  
Vol 56 (4) ◽  
pp. 535-543 ◽  
Author(s):  
Jenifer Nowatzki ◽  
Reginaldo Vieira de Sene ◽  
Katia Sabrina Paludo ◽  
Silvio Sanches Veiga ◽  
Constance Oliver ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Surface ◽  
Spider Venom ◽  
Venom Toxins ◽  
Brown Spider

scholarly journals Cryogenic technologies in biology and medicine

2020 ◽  
Vol 2020 (2b) ◽  
pp. 129-134
Author(s):  
O.I. Ossetsky ◽  
◽  
O.S. Snurnikov ◽  
Keyword(s):  
Public Health ◽  
Food Industry ◽  
Raw Materials ◽  
Biologically Active ◽  
Whole Body ◽  
Active Ingredients ◽  
Lipid Fractions ◽  
The World ◽  
New Generation

Cryogenic technologies in biology and medicine Current tendencies of the development of cryogenic technologies in the world practice and in Ukraine have been comparatively analyzed. A special attention has been paid to cryotechnologies in biology and medicine. The features of deriving the biologically active ingredients of natural raw materials by means of cryosublimation fractionation and extraction of lipid fractions with liquefied refrigerants, as well as perspectives of their application when obtaining the products of new generation in pharmacy, cosmetics, food industry have been considered. There were discussed the possibilities of human whole body extreme cryotherapy. Main aspects of its usage in practical public health have been considered.

scholarly journals New-generation osteoplastic materials based on biological and synthetic matrices

2021 ◽  
Vol 16 (1) ◽  
pp. 36-54
Author(s):  
D. D. Lykoshin ◽  
V. V. Zaitsev ◽  
M. A. Kostromina ◽  
R. S. Esipov
Keyword(s):  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Biologically Active ◽  
Side Reactions ◽  
Prolonged Release ◽  
Pharmaceutical Substances ◽  
Surgical Implants ◽  
Analytical Review ◽  
New Generation

Objectives. The purpose of this analytical review is to evaluate the market for osteoplastic materials and surgical implants, as well as study the features of new-generation materials and the results of clinical applications.Methods. This review summarizes the volumes of research articles presented in the electronic database PubMed and eLIBRARY. A total of 129 scientific articles related to biological systems, calcium phosphate, polymer, and biocomposite matrices as carriers of pharmaceutical substances, primary recombinant protein osteoinductors, antibiotics, and biologically active chemical reagents were analyzed and summarized. The search depth was 10 years.Results. Demineralized bone matrix constitutes 26% of all types of osteoplastic matrices used globally in surgical osteology, which includes neurosurgery, traumatology and orthopedics, dentistry, and maxillofacial and pediatric surgery. Among the matrices, polymer and biocomposite matrices are outstanding. Special attention is paid to the possibility of immobilizing osteogenic factors and target pharmaceutical substances on the scaffold material to achieve controlled and prolonged release at the site of surgical implantation. Polymeric and biocomposite materials can retard the release of pharmaceutical substances at the implantation site, promoting a decrease in the toxicity and an improvement in the therapeutic effect. The use of composite scaffolds of different compositions in vivo results in high osteogenesis, promotes the initialization of biomineralization, and enables the tuning of the degradation rate of the material.Conclusions. Osteoplastic materials of various compositions in combination with drugs showed accelerated regeneration and mineralization of bone tissue in vivo, excluding systemic side reactions. Furthermore, although some materials have already been registered as commercial drugs, a plethora of unresolved problems remain. Due to the limited clinical studies of materials for use on humans, there is still an insufficient understanding of the toxicity of materials, time of their resorption, speed of drug delivery, and the possible long-term adverse effects of using implants of different compositions.

scholarly journals TOXIC AND IMMUNE ALLERGIC RESPONSES OF ANT VENOM TOXINS: A REVIEW

2021 ◽  
pp. 1-7
Author(s):  
SIMRAN SHARMA ◽  
RAVI KANT UPADHYAY!
Keyword(s):  
Immune Responses ◽  
Biological Effects ◽  
Therapeutic Agents ◽  
Microbial Pathogens ◽  
Intense Pain ◽  
Venom Toxins ◽  
Potential Source ◽  
Ant Venom ◽  
Peptide Toxins ◽  
Promising Source

Present review article explains ant venom components and its allergic and biological effects in man and animals. Red ants or small fire ants secrete and inject venom very swiftly to defend their nest against predators, microbial pathogens, and competitors and to hunt the prey. Ant venom is a mixture of various organic compounds, including peptides, enzymes, and polypeptide toxins. It is highly toxic, allergic, invasive and venomous. It imposes sever paralytic, cytolytic, haemolytic, allergenic, pro-inflammatory, insecticidal, antimicrobial, and pain-producing pharmacologic activities after infliction. Victims show red ring-shaped allergic sign with regional swelling marked with intense pain. Ant venom also contains several hydrolases, oxidoreductases, proteases, Kunitz-like polypeptides, and inhibitor cysteine knot (ICK)-like (knottin) neurotoxins and insect defensins. Ant venom toxins/proteins generate allergic immune responses and employ eosinophils and produce Th2 cytokines, response. These compounds from ant venom could be used as a potential source of new anticonvulsants molecules. Ant venoms contain many small, linear peptides, an untapped source of bioactive peptide toxins. The remarkable insecticidal activity of ant venom could be used as a promising source of additional bio-insecticides and therapeutic agents.

scholarly journals Gas Separation Membranes upon Production of Functional Berry Juice

2019 ◽  
Vol 9 (1) ◽  
pp. 6616-6619
Keyword(s):  
Ascorbic Acid ◽  
Gas Separation ◽  
Biologically Active ◽  
Juice Quality ◽  
Active Components ◽  
Gas Separation Membrane ◽  
Separation Membranes ◽  
Gas Separation Membranes ◽  
Separation Membrane ◽  
New Generation

Nowadays manufacturing of new-generation food, including functional products, is especially important. Drinks based on fruits and berries are considered at present as optimum form of food required for organism replenishment with biologically active components and vitamins aiming at organism recovery. The retention of valuable components of fruits and berries is of great concern during such production. In order to produce functional juices, it is required to decrease oxygen amount upon their production in order to prevent destructive changes in drink, to improve its quality, to perform subsequent procedures and preservation. It is proposed to modify deaeration and to apply advanced equipment: deaerator with gas separation membrane which would allow to remove higher oxygen amount in comparison with the existing equipment while producing juices of predefined quality with high content of vitamins. The experiments were carried out with freshly squeezed black current juices using a vacuum unit, an Expert-009 analyzer of dissolved oxygen with optical sensor, a gas separation membrane. The juice quality was determined by its content of ascorbic acid (vitamin C) since it is destroyed under impact of oxygen. The content of ascorbic acid during storage was measured on juices produced by different deaeration procedures. The obtained experimental results proved efficiency of membrane application during juice production showing high content of vitamins. The content of ascorbic acid during storage was higher in the juice after deaeration using gas separation membrane in comparison with the juice without treatment and with the juice after vacuum deaeration. Therefore, the most efficient method to achieve the best removal of oxygen from juice is preheating of juice to 65°C with subsequent application of deaerator with gas separation membrane, subsequent packing and cooling of final product.

scholarly journals Brown Spider (Loxosceles) Venom Toxins as Potential Biotools for the Development of Novel Therapeutics

Toxins ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 355 ◽  
Author(s):  
Daniele Chaves-Moreira ◽  
Fernando Hitomi Matsubara ◽  
Zelinda Schemczssen-Graeff ◽  
Elidiana De Bona ◽  
Vanessa Ribeiro Heidemann ◽  
...  
Keyword(s):  
Serine Proteases ◽  
Three Dimensional ◽  
Biological Properties ◽  
Status Quo ◽  
Cellular Targets ◽  
Biological Features ◽  
Venom Toxins ◽  
The Status ◽  
Brown Spider ◽  
Systemic Manifestations

Brown spider envenomation results in dermonecrosis with gravitational spreading characterized by a marked inflammatory reaction and with lower prevalence of systemic manifestations such as renal failure and hematological disturbances. Several toxins make up the venom of these species, and they are mainly peptides and proteins ranging from 5–40 kDa. The venoms have three major families of toxins: phospholipases-D, astacin-like metalloproteases, and the inhibitor cystine knot (ICK) peptides. Serine proteases, serpins, hyaluronidases, venom allergens, and a translationally controlled tumor protein (TCTP) are also present. Toxins hold essential biological properties that enable interactions with a range of distinct molecular targets. Therefore, the application of toxins as research tools and clinical products motivates repurposing their uses of interest. This review aims to discuss possibilities for brown spider venom toxins as putative models for designing molecules likely for therapeutics based on the status quo of brown spider venoms. Herein, we explore new possibilities for the venom components in the context of their biochemical and biological features, likewise their cellular targets, three-dimensional structures, and mechanisms of action.

scholarly journals Mast Cells May Regulate The Anti-Inflammatory Activity of IL-37

2019 ◽  
Vol 20 (15) ◽  
pp. 3701 ◽  
Author(s):  
Theoharis C. Theoharides ◽  
Irene Tsilioni ◽  
Pio Conti
Keyword(s):  
Mast Cells ◽  
Immune Cells ◽  
Therapeutic Agents ◽  
Biologically Active ◽  
Inflammatory Activity ◽  
Allergic Reactions ◽  
Regulatory Cytokine ◽  
Inflammatory Processes ◽  
Anti Inflammatory ◽  
Novel Therapeutic

Mast cells are unique immune cells involved in allergic reactions, but also in immunity and inflammation. Interleukin 37 (IL-37) has emerged as an important regulatory cytokine with ability to inhibit immune and inflammatory processes. IL-37 is made primarily by macrophages upon activation of toll-like receptors (TLR) leading to generation of mature IL-37 via the action of caspase 1. In this review, we advance the premise that mast cells could regulate the anti-inflammatory activity of the IL-37 via their secretion of heparin and tryptase. Extracellular IL-37 could either dimerize in the presence of heparin and lose biological activity, or be acted upon by proteases that can generate even more biologically active IL-37 forms. Molecules that could selectively inhibit the secretion of mast cell mediators may, therefore, be used together with IL-37 as novel therapeutic agents.

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