Analysis of the Behaviour of Immunoglobulin G Antibodies in Children and Adults Convalescing From Severe Acute Respiratory Syndrome-Coronavirus-2 Infection

The pandemic caused by SARS CoV-2 (COVID-19) has affected millions of people since 2020. There are clinical differences and in mortality between the adult and paediatric population. Recently, the immune response through the development of antibodies has gained relevance due to the risk of reinfection and vaccines' development.Objective: Was to compare the association of clinical history and the clinical presentation of the disease with the development of IgG antibodies against SARS-CoV-2 in paediatric and adult patients with a history of positive reverse transcriptase-polymerase chain reaction (RT-PCR) results.Methods: Cross-sectional observational study carried out in a Paediatric Hospital in Mexico City included patients under 18 years of age and health personnel with positive RT-PCR for COVID-19 comparing antibody expression. The development of specific IgG antibodies was measured, the presence of comorbidities, duration, and severity of symptoms was determined.Results: Sixty-one subjects (20 < 18 years and 41 > 18 years) were analysed. The median sample collection was 3 weeks. There were no differences in the expression of specific antibodies; no differences were shown according to the symptoms' severity. A positive correlation (r = 0.77) was demonstrated between the duration of symptoms and antibody levels.Conclusions: In conclusion, there is a clear association between the duration of the symptoms associated with SARS-CoV-2 infection and the IgG units generated in paediatric and adult patients convalescing from COVID-19.

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Seroprevalence of varicella antibodies in adults without clinical history of disease

Cadernos de Saúde Pública ◽

10.1590/0102-311x00149119 ◽

2020 ◽

Vol 36 (1) ◽

Author(s):

Konrad Silva Büchele ◽

Danusa Ferreira Correa ◽

Mari Tuyama ◽

Alberto dos Santos de Lemos ◽

Marcellus Dias da Costa ◽

...

Keyword(s):

Travel Medicine ◽

Clinical History ◽

Cross Sectional Study ◽

Older Individuals ◽

Cross Sectional ◽

Varicella Zoster ◽

History Of Disease ◽

Protective Antibodies ◽

High Positive Predictive Value ◽

History Of

Abstract: Varicella in adults and immunocompromised patients can be severe. The clinical diagnosis of varicella has high accuracy and the history of disease has a high positive predictive value for protection. A significant portion of adults, however, cannot remember if they have had varicella, especially older individuals. We conducted a cross-sectional study to determine the seroprevalence of varicella protective antibodies titers in adults with no clinical history of disease, attended at a Reference Center for Special Immunobiologicals and Travel Medicine in Rio de Janeiro (Brazil). Titration of immunoglobulin G (IgG) antibodies to varicella-zoster was determined by chemiluminescence immunoassay. Among 140 adults without history of varicella, 92% had protective antibody titers. We concluded that seroprevalence of varicella-zoster protection was very high in adults with negative history of disease and the use of serology before vaccination reduced significantly unnecessary vaccine and immunoglobulin use.

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Cross-sectional quantitative RT-PCR study of feline coronavirus viremia and replication in peripheral blood of healthy shelter cats in Southern California

Journal of Feline Medicine and Surgery ◽

10.1177/1098612x17705227 ◽

2017 ◽

Vol 20 (4) ◽

pp. 295-301 ◽

Cited By ~ 7

Author(s):

Eric J Fish ◽

Pedro Paulo VP Diniz ◽

Yen-Chen Juan ◽

Frank Bossong ◽

Ellen W Collisson ◽

...

Keyword(s):

Peripheral Blood ◽

Southern California ◽

Buffy Coat ◽

Sample Collection ◽

Rt Pcr ◽

Case Control Studies ◽

M Gene ◽

Cross Sectional ◽

Qrt Pcr ◽

Feline Coronavirus

Objectives The objectives of this study were to determine the prevalence of feline coronavirus (FCoV) viremia, and its replication in peripheral blood using quantitative RT-PCR (qRT-PCR) methodology in a population of 205 healthy shelter cats in Southern California, as well as to assess any possible connection to longitudinal development of feline infectious peritonitis (FIP). Methods The study was performed on buffy-coat samples from EDTA-anticoagulated whole blood samples of 205 healthy shelter cats. From 50 of these cats, fecal samples were also examined. FCoV genomic and subgenomic RNA in the buffy coats was amplified by a total FCoV RNA qRT-PCR. Evidence for FCoV replication in peripheral blood and feces was obtained by M gene mRNA qRT-PCR. Results Nine of 205 cats (4.4%) were viremic by the total FCoV RNA qRT-PCR, and one of these cats had evidence of peripheral FCoV blood replication by an FCoV mRNA qRT-PCR. The single cat with peripheral blood replication had a unique partial M gene sequence distinct from positive controls and previously published FCoV sequences. Neither seven of the nine viremic cats with follow-up nor the single cat with replicating FCoV with positive qRT-PCR results developed signs compatible with FIP within 6 months of sample collection. Conclusions and relevance FCoV viremia and peripheral blood replication in healthy shelter cats have a low prevalence and do not correlate with later development of FIP in this study population, but larger case-control studies evaluating the prognostic accuracy of the qRT-PCR assays are needed.

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The Natural History of Adult Trigger Thumb

Journal of Hand Surgery (European Volume) ◽

10.1016/0266-7681(93)90120-5 ◽

1993 ◽

Vol 18 (2) ◽

pp. 247-248 ◽

Cited By ~ 6

Author(s):

C. B. SCHOFIELD ◽

N. D. CITRON

Keyword(s):

Natural History ◽

Average Duration ◽

Adult Patients ◽

Full Recovery ◽

Trigger Thumb ◽

Duration Of Symptoms ◽

History Of ◽

Average Loss ◽

Functional Reduction

30 consecutive adult patients presenting with trigger thumb (31 thumbs) were entered prospectively into a study to determine the natural history of the condition. Five patients insisted on treatment and could not be followed to resolution, but the rest resolved spontaneously after an average duration of symptoms of 6.8 months (range 2–15). There was a small but non-functional reduction in movement of the thumb in some of the patients: six lost an average of 7° of abduction and ten had an average loss of opposition of 1.4 (Kapandji grade). The remaining patients made a full recovery.

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The pathology of the ulnar nerve in acromegaly

Acta Endocrinologica ◽

10.1530/eje-08-0327 ◽

2008 ◽

Vol 159 (4) ◽

pp. 369-373 ◽

Cited By ~ 18

Author(s):

Alberto Tagliafico ◽

Eugenia Resmini ◽

Raffaella Nizzo ◽

Lorenzo E Derchi ◽

Francesco Minuto ◽

...

Keyword(s):

Ulnar Nerve ◽

Clinical History ◽

Cubital Tunnel Syndrome ◽

Cubital Tunnel ◽

Control Group ◽

Cross Sectional ◽

Sensory Disturbances ◽

History Of ◽

Tunnel Syndrome ◽

Written Informed Consent

ContextAcromegalic patients may complain of sensory disturbances in their hands. Cubital tunnel syndrome, the ulnar nerve neuropathy at the cubital tunnel (UCT), in acromegalic patients has never been reported.ObjectiveTo describe and assess the prevalence of UCT in acromegalic patients and the effects of 1 year of therapy on UCT.PatientsWe examined prospectively 37 acromegalic patients with no history of polyneuropathy, acute trauma at the elbow, no diabetes or hypothyroidism with clinical examination, nerve conduction studies (NCS), and high-resolution ultrasound (US). A control group was made by 50 volunteers. The local ethics committee approved the study and written informed consent was obtained from all subjects involved in the study.InterventionClinical history, physical examination, NCS, and US were used to diagnose UCT at the beginning of the study and after 1 year.ResultsIn 8 of 37 patients, a diagnosis of UCT was made at the beginning of the study reflecting a prevalence of 21%. After 1 year, 5 of 8 (62.5%) patients reported clinical and NCS improvements and evident US reduction of nerve cross-sectional area (CSA; 16.7±2.9 mm2 vs 12.2±3.1 mm2; P<0.001). In 3 of 8 (37.5%) patients, the UCT was unchanged. Ulnar nerve CSA was significantly increased in acromegalic patients with UCT (16.7±2.9 mm2 vs 11.1±2.3 mm2; P<0.047).ConclusionUlnar neuropathy could occur in acromegalic patients and can improve in 62% of cases with disease control. Due to the different management and therapeutic approach, it would be important to make differential diagnosis between cubital and carpal tunnel syndrome in acromegaly.

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Risk factors related to childbirth in El Salvador

ALERTA Revista Científica del Instituto Nacional de Salud ◽

10.5377/alerta.v2i2.7922 ◽

2019 ◽

Vol 2 (2) ◽

pp. 144-152

Author(s):

Fátima Larisa Chavarría Rodríguez

Keyword(s):

Public Health ◽

Risk Factors ◽

Preterm Birth ◽

El Salvador ◽

Multiple Pregnancy ◽

Public Health Problem ◽

Clinical History ◽

Cross Sectional ◽

History Of ◽

Prevalence Ratios

Introduction. Preterm birth is a public health problem, considered a high risk factor for morbidity, disability and neonatal mortality. Objective. Characterize risk factors related to preterm birth in El Salvador. Methodology. Analytical cross-sectional study of risk factors related to preterm birth, in women who received childbirth care in the national public health system, during 2017. Data was obtained from the Perinatal Information System. 44 891 cases were analyzed. Qualitative variables were analyzed through absolute values and frequencies, and the quantitative variables, through absolute values, frequency and central tendency measures, using the Statistical Package for the Social Science (SPSS) version 24 program. The analysis of relation of variables, was performed with the Epidat version 3.1 program, through the calculation of prevalence ratios (PR) and the calculation of Odds Ratio (OR), both analysis with a 95% confidence interval and a value of p <0.05. Results. 9.5% of the deliveries attended were preterm. The risk analysis through the calculation of prevalence ratios showed that women with a multiple pregnancy were 14 times more likely to have a preterm birth. Preterm birth was 4.7 times more frequent in women with a clinical history of diabetes mellitus and 2.4 times more frequent, with a history of high blood pressure. Conclusions. Women who have a multiple pregnancy and pregnant women with a clinical history of chronic diseases, and an obstetric history of preeclampsia, are more likely to have a preterm birth. Likewise, obesity and inadequate age for pregnancy, mainly women over 35 years, are also related to preterm birth.

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ASSOCIATION OF SEROPOSITIVITY FOR CMV IGM AND IGG WITH ADVERSE PREGNANCY OUTCOME

Pakistan Armed Forces Medical Journal ◽

10.51253/pafmj.v71i3.2928 ◽

2021 ◽

Vol 71 (3) ◽

pp. 831-35

Author(s):

Ambreen Amna ◽

Farkhunda Nadeem ◽

Amin Fahim ◽

Altaf Hussian Jatoi ◽

Kanwal Abbas ◽

...

Keyword(s):

Pregnancy Outcome ◽

Adverse Pregnancy Outcome ◽

Cross Sectional Study ◽

Reproductive Age ◽

University Hospital ◽

Igg Antibodies ◽

Cross Sectional ◽

History Of ◽

Adverse Pregnancy ◽

Reproductive Age Group

Objective: To find out the prevalence of antibodies against cytomegalovirus and their association with adverse pregnancy outcome in women, at Isra University Hospital Hyderabad. Study Design: Cross-sectional study. Place and Duration of Study: Department of Gynaecology & Obstetrics, Isra University Hospital (IUH) Hyderabad, from Jan to Jun 2018. Methodology: Cytomegalovirus IgM and IgG antibodies in 305 women of reproductive age group were measured using the Enzyme Linked Fluorescent Assaysystem kit. Results: The combined positivity of anti cytomegalovirus IgG and IgM antibodies was 93 (30.40%). About 37 (37.37%) and 28 (34.14%) women were found to be seropositive for cytomegalovirus antibodies who had history of spontaneous miscarriage and recurrent miscarriages respectively. Conclusion: Higher association of seropositivity for cytomegalovirus IgM and IgG with bad obstetrical history and low economy was found.

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Seroepidemiological Study of Novel Corona Virus (CoVID-19) in Tehran, Iran

10.1101/2021.01.18.20248911 ◽

2021 ◽

Author(s):

Zeinab Tabanejad ◽

Sorena Darvish ◽

Zeinab Borjian Boroujeni ◽

Seyed Saeed Asadi ◽

Morteza Mesri ◽

...

Keyword(s):

False Negative ◽

Underlying Disease ◽

Population Based ◽

Immunological Methods ◽

Age Group ◽

Igg Antibodies ◽

Rt Pcr ◽

Igg Antibody ◽

Cross Sectional ◽

Exposed Population

AbstractA novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has now spread to all countries of the world, including Iran. Although new anti-coronavirus antibodies in patients may be identified by immunological methods with sufficient sensitivity and specificity, the conclusive diagnosis of the disease is by the molecular RT-PCR process. We used a population-based seroepidemiological survey to quantify the proportion of the exposed population with SARS-CoV-2 antibodies and evaluated whether the antibodies are a marker of total or partial immunity to the proportion of the population that remains susceptible to the virus. This cross-sectional study was conducted to investigate the seroprevalence of CoVID-19 in Tehran, the capital of Iran, between April and end of October 2020. Specimens of clotted and heparinized blood (2ml) were collected from the patients. The serum and plasma were separated and stored at − 80LJ°C until use. We examined serum anti-SARS-CoV-2 IgG and IgM antibodies from 1375 in-patients admitted to our hospitals using ELISA kits. In total, 1375 participants were enrolled in this study, and SARS-CoV-2 antibodies were detected using IgM-IgG antibody assay in 291 patients. Among the seropositive patients studied, 187 were men (64.3%), and 104 were women (35.7%) (P<0.05). The mean age of the patients was 49±8.4 years; the majority (27%) were in age group 31-40 years. Also, the lowest frequency of cases was reported in the age group of 1-10 years (P <0.05). We determined the seroprevalence of SARS-CoV-2 for IgM or IgG antibodies to be 21.2%. Diabetes mellitus was the most common underlying disease among SARS-CoV-2 patients [P=0.05; Odd Ratio=1.61(0.90-2.91)]. Conventional serological assays in SARS-CoV-2 cases, such as the enzyme-linked immunoassay (ELISA) for specific IgM and IgG antibodies, have a high-throughput advantage and minimize false-negative rates that occur with the RT-PCR method. This study determined the seroprevalence of SARS-CoV-2 antibodies to be 21%. Control of diabetes among other cases factors shall play important role in management and control of COVID-19.

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Evaluation of elderly patients with hearing impairment.

The Professional Medical Journal ◽

10.29309/tpmj/2020.27.10.4071 ◽

2020 ◽

Vol 27 (10) ◽

pp. 2154-2158

Author(s):

Syed Ali Naqi ◽

Syed Maisam Ali ◽

Arslan Akhtar ◽

Tabassum Aziz ◽

Tahira Sajid ◽

...

Keyword(s):

Hearing Impairment ◽

Past History ◽

Clinical History ◽

The Elderly ◽

Cross Sectional Study ◽

Variable Degree ◽

Cross Sectional ◽

Detailed Clinical History ◽

History Of ◽

Study Setting

Hearing is decreased in later age is due to aging and is normally seen in most of the elderly people all over the world. Objectives: To ascertain the degree of hearing in elders in relation to age, pattern and extent of hearing weakness in old age so that interventional strategies can be planned to improve their wellbeing. Study Design: Cross Sectional Study. Setting: ENT Outdoor Clinic of Akbar Niazi Teaching Hospital of Islamabad Medical and Dental College, Bhara Kahu, Islamabad. Period: December 2017 to October 2018. Material & Methods: 200 patients were part of the study with age > 50 years and no past history of drug therapy. Data was gathered by detailed clinical history, clinical examination and audiometric findings. Result: 57% patients were found with normal hearing and 43% had variable degree of hearing deterioration. Highest number of hearing weakness was found in 9th decade. Most of hearing deterioration was found in both the ears and that of sensorineural in type. Conclusion: Sensorineural type of hearing impairment is very frequently occurring problem seen in the latter part of life in our community. The frequency and severity of decreased hearing is directly proportional to age.

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Evaluation of nine serological rapid tests for the detection of SARS-CoV-2

Revista Panamericana de Salud Pública ◽

10.26633/rpsp.2020.149 ◽

2020 ◽

Vol 44 ◽

pp. 1

Author(s):

Marcela Mercado ◽

Jeadran Malagón-Rojas ◽

Gabriela Delgado ◽

Vivian Vanesa Rubio ◽

Lida Muñoz Galindo ◽

...

Keyword(s):

Study Groups ◽

Cross Sectional Study ◽

Igg Antibodies ◽

Sectional Study ◽

Rt Pcr ◽

Serum Samples ◽

Serological Tests ◽

Cross Sectional ◽

Diagnostic Aid ◽

Rapid Tests

Objective. To evaluate the operative capacity of nine serological rapid tests to detect the IgM/IgG antibodies response in serum from patients with SARS-CoV-2 in different clinical stages. Methods. A cross-sectional study of serological rapid tests was designed to compare the performance of the evaluated immunochromatographic tests for the diagnosis of SARS-CoV-2. A total of 293 samples was used, including negatives, asymptomatic, and symptomatic serum samples. Results. The sensitivity of the evaluated tests was low and moderate in the groups of asymptomatic serum samples and the group of serums coming from patients with less than 11 days since the onset of the symptoms. The specificity for the anti-SARS-CoV-2 antibodies tests ranged between 86.5%-99% for IgM and 86.5%-99.5% for IgG. The sensitivity and the likelihood ratio were different according to the study groups. The usefulness of these tests is restricted to symptomatic patients and their sensitivity is greater than 85% after 11 days from the appearance of symptoms. Conclusions. Serological tests are not an adequate strategy for the identification of asymptomatic and pre-symptomatic patients. Serological rapid tests for the detection of specific anti-SARS-CoV-2 antibodies can be used as a diagnostic aid, but diagnosis must be confirmed by RT-PCR. Rapid tests should be reserved for patients with symptoms lasting more than 11 days.

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Measles antibody seropositivity among children with allergic diseases: A cross-sectional study in the Japan Environment & Children’s Pilot Study

PLoS ONE ◽

10.1371/journal.pone.0257721 ◽

2021 ◽

Vol 16 (9) ◽

pp. e0257721

Author(s):

Mayako Saito-Abe ◽

Kiwako Yamamoto-Hanada ◽

Kensuke Shoji ◽

Miori Sato ◽

Makoto Irahara ◽

...

Keyword(s):

Pilot Study ◽

Pediatric Population ◽

Clinical History ◽

Allergic Diseases ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Antibody Positivity ◽

History Of ◽

The Relationship

Background The relationship between allergic individuals and their responsiveness to routine vaccines has rarely been investigated. This study examined whether the seroprevalence of measles antibody differed between children with and without allergic diseases in the general pediatric population. Methods The cross-sectional study was performed within a prospective general birth cohort (a pilot study of the Japan Environment & Children’s Pilot Study [JECS]) of children aged 8 years. The clinical history of allergic diseases, measles, and the concentration of measles immunoglobulin G titers in serum enzyme immunoassay were examined. Fisher’s exact tests were used to assess the relationships between the allergic characteristics of the children and their measles antibody positivity rates. Results This study included 162 children. Any allergic disease was reported in 75 (46.3%). The measles antibody positivity rate was 94.7% among children with any allergic diseases and 92.0% among children without allergic diseases. Our results revealed no differences in measles antibody seropositivity between children with allergies and controls. Conclusions Children with allergies mount and maintain a comparable immune response to the measles vaccine.

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