scholarly journals Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis

2021 ◽  
Vol 12 ◽  
Author(s):  
José Marcos dos Santos Oliveira ◽  
Théo Fortes Silveira Cavalcanti ◽  
Ingrid Ferreira Leite ◽  
Dávida Maria Ribeiro Cardoso dos Santos ◽  
Isabel Cristina Celerino de Moraes Porto ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Water Solubility ◽  
Composite Resin ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Composite Resins ◽  
Control Group ◽  
Average Roughness ◽  
3T3 Fibroblasts ◽  
Physical Chemical

The aim of this study was to obtain a Brazilian red propolis (BRP) enriched composite resin and to perform the characterization of its antibacterial activity, mechanical, and physical-chemical properties. Brazilian red propolis ethyl acetate extract (EABRP) was characterized by LC-ESI-Orbitrap-FTMS, UPLC-DAD, antibacterial activity, total flavonoids content, and radical scavenging capacity. BRP was incorporated to a commercial composite resin (RC) to obtain BRP enriched composite at 0.1, 0.15 and 0.25% (RP10, RP15 and RP25, respectively). The antibacterial activity RPs was evaluated against Streptococcus mutans by contact direct test and expressed by antibacterial ratio. The RPs were characterized as its cytotoxicity against 3T3 fibroblasts, flexural strength (FS), Knoop microhardness (KHN), post-cure depth (CD), degree of conversion (DC%), water sorption (Wsp), water solubility (Wsl), average roughness (Ra), and thermal analysis. Were identified 50 chemical compounds from BRP extract by LC-ESI-Orbitrap-FTMS. EABRP was bacteriostatic and bactericide at 125 and 500 μg/ml, respectively. The RP25 exhibited antibacterial ratio of 90.76% after 1 h of direct contact with S. mutans (p < 0.0001) while RC no showed significative antibacterial activity (p = 0.1865), both compared with cell control group. RPs and RC no showed cytotoxicity. RPs exhibited CD from 2.74 to 4.48 mm, DC% from 80.70 to 83.96%, Wsp from 17.15 to 21.67 μg/mm3, Wsl from 3.66 to 4.20 μg/mm3, Ra from 14.48 to 20.76 nm. RPs showed thermal resistance between 448–455°C. The results support that propolis can be used on development of modified composite resins that show antibacterial activity and that have compatible mechanical and physical-chemical properties to the indicate for composite resins.

The Effect of Finishing and Polishing Procedures on the Surface Roughness of Composite Resin Materials

2007 ◽  
Vol 8 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Nuray Attar
Keyword(s):  
Surface Roughness ◽  
Composite Resin ◽  
Resin Composite ◽  
Composite Resins ◽  
Control Group ◽  
Surface Groups ◽  
Average Roughness ◽  
Strip Surface ◽  
Polishing Procedures ◽  
Surface Sealant

Abstract Aims The aims of this study were to evaluate the effect of various finishing and polishing procedures on the surface roughness of six different composite resin materials (Artemis Enamel, TPH Spectrum, Filtek A–110, Filtek Supreme Enamel, Solitaire 2, and Filtek P–60) as well as to evaluate the effectiveness of the surface sealant application (BisCover) on the surface roughness after finishing and polishing procedures of tested composites. Methods and Materials Specimens (n=168) measuring 5 mm in diameter x 2 mm in thickness were fabricated in a plexiglass well covered with a Mylar strip using six composite resins. A control group of seven specimens of each material received no polishing after being cured under the Mylar strip. Twenty-one specimens for each composite were randomly divided among three finishing and polishing groups (n=7). Each group was polished using a different system: Carbide bur/Sof-Lex disc, Carbide bur/Enhance disc with polishing paste, and Carbide bur/Edenta composite finishing kit. The average surface roughness (Ra, ìm) of the control and treated specimens were measured with the Mitutoyo Surftest–402 Surface Roughness tester. After a surface sealant (BisCover) was applied to all treated specimens, according to manufacturer's instructions, the average roughness (Ra) was measured again. Results were statistically analyzed using analysis of variance (ANOVA) and the post-hoc Scheffe's test at a p<0.05 significance level. Results Significant differences were found for the surface roughness (p<0.05) with interaction among composite resins and the finishing systems used (p<0.05). Enhance/Biscover finishing and polishing procedure surface was not significantly different from the Mylar strip surface groups (p>0.05). The Mylar strip group was not significantly different from the Sof-Lex/BisCover and Edenta/BisCover groups. The ranking of mean Ra values by materials was as follows: Filtek Supreme Enamel < Filtek A110 < TPH Spectrum < Artemis <Filtek P–60 < Solitaire 2. The ranking of mean Ra values by polishing systems was as follows: Enhance/BisCover < Mylar Strip < Sof-Lex/BisCover < Edenta/BisCover < Sof-Lex < Enhance < Edenta. Conclusion Smoother surfaces were recorded for the Enhance/BisCover and the Mylar strip-formed surface groups. The composite finishing kit Edenta significantly increased the Ra for all tested composites (p<0.05). But after finishing with Edenta, the use of a surface sealant (BisCover) significantly improved the surface smoothness of all tested composites (p<0.05). Use of BisCover surface sealant on anterior and posterior resin composite restorations after finishing and polishing procedures is recommended. Citation Attar N. The Effect of Finishing and Polishing Procedures on the Surface Roughness of Composite Resin Materials. J Contemp Dent Pract 2007 January;(8)1:027-035.

Influence of Hydrogen Peroxide Bleaching Gels on Color, Opacity, and Fluorescence of Composite Resins

2012 ◽  
Vol 37 (5) ◽  
pp. 526-531 ◽  
Author(s):  
CRG Torres ◽  
CF Ribeiro ◽  
E Bresciani ◽  
AB Borges
Keyword(s):  
Hydrogen Peroxide ◽  
Composite Resin ◽  
Color Change ◽  
Composite Resins ◽  
Control Group ◽  
Peroxide Bleaching ◽  
Color Changes ◽  
Tukey Test ◽  
Bleaching Gels ◽  
Bleaching Treatment

SUMMARY The aim of the present study was to evaluate the effect of 20% and 35% hydrogen peroxide bleaching gels on the color, opacity, and fluorescence of composite resins. Seven composite resin brands were tested and 30 specimens, 3-mm in diameter and 2-mm thick, of each material were fabricated, for a total of 210 specimens. The specimens of each tested material were divided into three subgroups (n=10) according to the bleaching therapy tested: 20% hydrogen peroxide gel, 35% hydroxide peroxide gel, and the control group. The baseline color, opacity, and fluorescence were assessed by spectrophotometry. Four 30-minute bleaching gel applications, two hours in total, were performed. The control group did not receive bleaching treatment and was stored in deionized water. Final assessments were performed, and data were analyzed by two-way analysis of variance and Tukey tests (p&lt;0.05). Color changes were significant for different tested bleaching therapies (p&lt;0.0001), with the greatest color change observed for 35% hydrogen peroxide gel. No difference in opacity was detected for all analyzed parameters. Fluorescence changes were influenced by composite resin brand (p&lt;0.0001) and bleaching therapy (p=0.0016) used. No significant differences in fluorescence between different bleaching gel concentrations were detected by Tukey test. The greatest fluorescence alteration was detected on the brand Z350. It was concluded that 35% hydrogen peroxide bleaching gel generated the greatest color change among all evaluated materials. No statistical opacity changes were detected for all tested variables, and significant fluorescence changes were dependent on the material and bleaching therapy, regardless of the gel concentration.

scholarly journals Effect of irradiation mode and filling technique on resin/dentin bonding strength in class I cavities

2004 ◽  
Vol 18 (3) ◽  
pp. 260-265 ◽  
Author(s):  
Alex José Souza dos Santos ◽  
Marcelo Giannini ◽  
Luis Alexandre Maffei Sartini Paulillo ◽  
José Roberto Lovadino ◽  
Ricardo Marins de Carvalho
Keyword(s):  
Bonding Strength ◽  
Composite Resin ◽  
Composite Resins ◽  
Class I ◽  
Control Group ◽  
Human Teeth ◽  
Dentin Bonding ◽  
Soft Start ◽  
Microtensile Testing ◽  
Dunnett's Test

Factors such as light-curing mode, filling technique and cavity configuration may affect the bonding strength to dentin. This study evaluated the effect of irradiation mode and filling technique on resin/dentin bonding strength on the buccal wall of class I cavities in human teeth. Occlusal enamel was removed to expose a flat dentin surface. Occlusal cavities (4 x 3 x 3 mm) were prepared in dentin. The adhesive Single Bond was applied according to the manufacturer's instructions and TPH Spectrum composite resin was placed using the following: oblique incremental, horizontal incremental or bulk filling techniques. The composite resin was light-cured either by continuous (600 mW/cm² for 40 s) or Soft-Start (250 mW/cm² for 10 s + 600 mW/cm² for 30 s) modes. Specimens of the control group were obtained by bonding the material to the flat exposed buccal wall of the cavity (C-factor = 1). The teeth were stored in water at 37ºC for 24 h and prepared for microtensile testing. Bonded beams of approximately 0.8 mm² were obtained from the buccal wall and tested with a tension of 0.5 mm/min. Results were analyzed by two-way ANOVA, Tukey's test and Dunnett's test (alpha = 0.05). Incremental placement techniques with both irradiation modes produced higher bonding strength values than the bulk technique (p < 0.05). Bonding strength tested in the cavities had lower values than those obtained in flat dentin surfaces (control group) (p < 0.05), except for incremental fillings using stepped irradiation. Bonding strength to the cavity walls depends on the filling technique and on the irradiation mode of composite resins.

scholarly journals Pengaruh kopi arabika terhadap perubahan warna resin komposit hybrid

e-GIGI ◽  
2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Style V. Sirang ◽  
P. S. Anindita ◽  
. Juliatri
Keyword(s):  
Hybrid Composite ◽  
Composite Resin ◽  
Composite Resins ◽  
Control Group ◽  
Control Group Design ◽  
Arabica Coffee ◽  
Group Design ◽  
Hybrid Type ◽  
Post Test ◽  
One Way Anova

Abstract: Composite resin is one of the restorations used in the field of dentistry due to its good esthetic property. To date, the most commonly used composite resin is the hybrid type. Its weakness is the nature of fluid absorbance that can change its color. This discoloration can caused inter alia by food and beverage consumption. Arabica coffee is mostly consumed by our society, however, it has deposit of chlorogenic acid that might cause discoloration. This study was aimed to assess the influence of arabica coffee to the discoloration of hybrid composite resins. This was a true experimental study with a pre test and post test only control group design. Total samples were 24 composite resins of hybrid type (diameter 5 mm and height 2 mm). Each treatment group consisted of 6 samples. The samples were divided into 4 treatment groups, as follows: 0 day, 3 days, 5 days, and 7 days of immersion in arabica coffee solution. The one-way anova test showed that the influence of arabica coffee solution to discoloration of composite resins was significant (p=0.00). Conclusion: There was a significant influence of the duration of immersion in arabica coffee to discoloration of hybrid composite resinsKeywords: resin composite, discoloration, arabica coffee Abstrak: Resin komposit merupakan salah satu bahan restorasi yang sering digunakan di bidang kedokteran gigi karena memiliki nilai estetik yang baik. Saat ini jenis resin komposit yang sering digunakan yaitu resin komposit tipe hybrid. Resin komposit memiliki kekurangan yaitu sifat absorbansi cairan yang dapat menyebabkan perubahan warna. Salah satu penyebab perubahan warna yaitu makanan dan minuman. Kopi arabika merupakan minuman yang sering dikonsumsi oleh masyarakat dan memiliki kandungan asam klorogenik yang dapat memengaruhi perubahan warna. Penelitian ini bertujuan untuk mengetahui pengaruh kopi arabika terhadap perubahan warna resin komposit hybrid. Jenis penelitian ialaj eksperimental murni dengan pre test dan post test only control group design. Jumlah sampel penelitian yaitu 24 resin komposit tipe hybrid dengan ukuran diameter 5 mm dan tinggi 2 mm, dibagi dalam tiga kelompok. Masing-masing kelompok perlakuan terdiri dari 6 sampel. Sampel dibagi menjadi 4 kelompok yaitu kelompok perlakuan 0 hari, 3 hari, 5 hari, dan 7 hari perendaman dalam larutan kopi arabika. Hasil analisis data dengan uji one way Anova menunjukkan adanya pengaruh kopi arabika terhadap perubahan warna resin komposit (p=0,00). Simpulan: Terdapat pengaruh bermakna dari lama perendaman dalam kopi arabika terhadap perubahan warna resin komposit hybrid. Kata kunci : resin komposit, perubahan warna, kopi arabika

scholarly journals Curcumin-Functionalized Gelatin Films: Antioxidant Materials with Modulated Physico-Chemical Properties

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1824
Author(s):  
Katia Rubini ◽  
Elisa Boanini ◽  
Silvia Parmeggiani ◽  
Adriana Bigi
Keyword(s):  
Water Solubility ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Crosslinking Agent ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Mechanical Tests ◽  
Scanning Calorimetry ◽  
Gelatin Films ◽  
Physico Chemical

In this paper we used curcumin as a functionalizing agent of gelatin films with the aim to get antioxidant materials with modulated physico-chemical properties. To this aim, we prepared gelatin films at different contents of curcumin up to about 1.2 wt%. The as-prepared films, as well as glutaraldehyde crosslinked films, were submitted to several tests: swelling, water solubility, differential scanning calorimetry, X-ray diffraction, mechanical tests and curcumin release. The radical scavenging activity of the as-prepared films is similar to that of free curcumin, indicating remarkable antioxidant properties. All the other tested properties vary as a function of curcumin content and/or the presence of the crosslinking agent. In particular, the films exhibit sustained curcumin release in different solvents. Thanks to its biocompatibility, biodegradability and lack of antigenicity, gelatin uses span from food processing to packaging and biomaterials. It follows that the modulated properties exhibited by the functionalized materials developed in this work can be usefully employed in different application fields.

scholarly journals PERBANDINGAN KEBOCORAN TEPI ANTARA RESTORASI RESIN KOMPOSIT TIPE BULK-FILL DAN TIPE PACKABLE DENGAN PENGGUNAAN SISTEM ADHESIF TOTAL ETCH DAN SELF ETCH

2016 ◽  
Vol 3 (1) ◽  
pp. 8
Author(s):  
Arlina Nurhapsari
Keyword(s):  
Composite Resin ◽  
Chemical Properties ◽  
Adhesive System ◽  
Composite Resins ◽  
Blue Dye ◽  
Dentin Bonding ◽  
Physico Chemical ◽  
System Method ◽  
Different Types ◽  
Self Etch

Background: Composite resin has been widely used because of it's great aesthetic and physico-chemical properties. Type of posterior composite resins mostly used nowadays are packable and bulk-fill composite resins. Shrinkage in composite resin resulting microleakage that could lead to pulp sensitivity. Dentin bonding adhesive system can help to minimalize shrinkage. There are two type of adhesive system, the total etch and self etch group. The purpose of this study was to compare the microleakage between bulk-fill and packable composit resin with two different types of dentin bonding adhesive system. Method: twenty premolar teeth used as sample. The teeth were divided into four groups of 5 teeth each and Class I cavity preparations were prepared. The groups are : G1 (PC + TE), G2 (PC + SE), G3 (BC + TE), G4 (BC + SE). The specimens were thermocycled, stained with methylene blue dye, and sectioned to evaluate the dye penetration.Result: Kruskal Wallis test showed no difference among 4 groups. Meanwhile, Mann Whitney test showed significance difference between G2 - G3, and G3 - G4.Conclus/on: The study demonstrated that total etch has better sealing ability than self etch and there was no significant microleakage difference between bulkfill composite and packable composite.

scholarly journals A Preliminary Research Study on the Cytotoxicity of Expired and Non-expired Composite Resins: In Vitro Study

2020 ◽  
pp. 227-238
Author(s):  
Magrur Kazak DDS, PhD ◽  
Nazmiye Donmez DDS, PhD ◽  
Fatemeh Bahadori PhD ◽  
Vildan Betul Yenigun PhD ◽  
Abdurrahim Kocyigit MD, PhD
Keyword(s):  
Cell Viability ◽  
Composite Resin ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Composite Resins ◽  
Control Group ◽  
Significant Difference ◽  
Preliminary Research

Objective: Studies have focused on use of non-expired composites. Unfortunately some clinicians still use expired composite resins without considering their effects. The objective of this in vitro preliminary research was to investigate cytotoxicity of expired(6-months) and non-expired composite resins. Materials and methods: Expired (E) and non-expired (NE) samples of one bulk-fill (Tetric N-Ceram Bulk-fill [TNB], Ivoclar Vivadent), two nano-hybrid (Tetric N-Ceram [TN], Ivoclar Vivadent; Clearfil Majesty ES-2 [CM], Kuraray) composite resins were tested on L929 fibroblast cells. Medium covering cells was removed then plastic rings (2-mm height) were filled with non-polymerized composite resins, placed in direct contact with cells and polymerized with LED light curing unit (LCU). Three samples were prepared for each group. After polymerization, removed medium was added to the cells. Cells that were left without medium (WOM) and cells that were exposed to LCU were used as positive control groups. Cells without any treatment were used as negative control group (C). Cells were incubated with tested materials for 7-days to evaluate cytotoxicity. Cell viability was calculated by sulforhodamine B test as a percentage (%). One-way ANOVA and post-hoc Tukey tests were used for statistical analyses (p<0.05). Results: Comparison between E and NE groups of same composite resins did not result in statistically significant differences (p>0.05), except between TN NE and TN E (p<0.05). TN E group was significantly more cytotoxic than TN NE group. When NE composite resin groups were compared to each other, statistically significant difference was only obtained between TNB NE and TN NE (p<0.05). Among all tested groups, TN NE group showed the least cytotoxic profile. No statistically significant differences were determined when E composite resin groups were compared to each other (p>0.05). All experimental groups compared with C group showed statistically significant cytotoxicity (p<0.05). A statistically significant difference existed between LCU and C groups (p<0.05). Conclusions: In clinical practice, expired composite resins should never be used. Although a correlation was found between expiration dates of nano-hybrid composite resins and cell viability, opposite data were obtained for bulk-fill composite resin. Researches are still required to evaluate biocompatibility of bulk-fill composite resins at various thicknesses with current LCUs.

scholarly journals INFLUÊNCIA DOS ENXAGUATÓRIOS BUCAIS NA RUGOSIDADE SUPERFICIAL DE UMA RESINA COMPOSTA

2016 ◽  
Vol 7 (4) ◽  
Author(s):  
Gabriela Sampaio Aragão ◽  
Renata Martins Falcão ◽  
Íris Durães ◽  
Rebeca Barroso Bezerra
Keyword(s):  
Composite Resin ◽  
Control Group ◽  
Internal Diameter ◽  
Distilled Water ◽  
Average Roughness ◽  
Significance Level ◽  
Dental Biofilm ◽  
Mouth Rinses ◽  
Significant Difference ◽  
Composite Resin Restorations

Surface smoothness is an important feature of restorative material. Thus, composite roughness levels should be low to make retention of dental biofilm. Alterations of the surface of composite resin restorations can be associated to drinks and acidic foods, components of saliva, finishing and polishing methods, as well as the action of bleaching agents, rinses and fluoride. Purpose: To evaluate the effect of mouth rinses in superficial roughness of composite resin through a quantitative evaluation. Methods: 48 specimens with composite resin FiltekTM Z350 XT were made and divided into 4 groups (n=12): distilled water, Colgate® Plax, Oral B® and Listerine®. Specimens were made using a rectangular matrix with holes, each measuring 0.8 cm of internal diameter and 0.2 cm in thickness. The specimens were immersed in 50 ml of each substance, for 12 hours, to simulate a year of daily use of the solution for 2 minutes. Then specimens were stored in distilled water until reading of the roughness tester. Data were statistically treated by ANOVA and Tukey tests, with a significance level of 95%. Results: No statistically significant difference was observed between the control group and Listerine® (p = 0.03). When comparing the average roughness of different brands of mouthwashes, there was no statistically significant difference (p > 0.05). Conclusions: Among the mouthwashes tested, only Listerine® caused statistically significant increase in superficial roughness of the composite resin used.

scholarly journals Effect of a Prebrushing Mouthwash on Color Stability of Composite Resins

2021 ◽  
Vol 22 (3) ◽  
pp. 191-194
Author(s):  
Ângela Dalla Nora ◽  
Camila da Silva Rodrigues ◽  
Rachel de Oliveira Rocha
Keyword(s):  
Composite Resin ◽  
Color Change ◽  
Test Group ◽  
Color Stability ◽  
Composite Resins ◽  
Control Group ◽  
Water Control ◽  
Dental Tissues ◽  
Evaluation Time ◽  
Materials Used

AbstractDespite composite resins capacity to mimic dental tissues, they can discolor over time. However, there is no information on the effect of prebrushing mouthwashes on the composite resin staining. This study aimed to evaluate the effect of prebrushing mouthwashes on the color stability of three composite resins. Twenty specimens of each resin were prepared: Filtek Z250 (3M ESPE –S Paul, MN, USA), Charisma (Heraeus Kulzer GmbH - Hanau - Germany) e EvoluX (Dentsply- Petrópolis - Brazil). Groups were randomly divided accord to the treatment: immersion in prebrushing mouthwash (test group) or immersion in deionized water (control group). Test groups were immersed in 10 ml of prebrushing mouthwash Plax Magic (Colgate-Palmolive, São Bernardo do Campo, SP, Brazil) for 60 s three times a day during 35 days following the manufacturer’s instructions. Color measurements were performed after 24 h of specimen’s preparation and after 7, 14, 21, and 35 days of immersion time. Results showed that prebrushing mouthwash Plax Magic promoted a color change in all the tested materials and evaluation time. The color change was greater in composite resin Charisma, followed by Z250 and EvoluX. Therefore, prebrushing mouthwash promoted a color change in all the materials used. Composite resin Charisma demonstrated less color stability than Filtek Z250 resin and EvoluX resin, which was the most stable.Keywords: Composite Resins. Oral Hygiene. Color.ResumoApesar da capacidade das resinas compostas de mimetizar os tecidos dentais, esses materiais podem ter sua cor alterada com o tempo. No entanto, não há informação na literatura sobre o efeito de evidenciadores de placa na estabilidade de cor desses materiais. Este estudo teve por objetivo avaliar o efeito de um evidenciador de placa na estabilidade de cor e três resinas compostas. Para isso, 20 espécimes de cada resina foram preparados: Filtek Z250 (3M ESPE –S Paul, MN, EUA), Charisma (Heraeus Kulzer GmbH - Hanau - Alemanha) e EvoluX (Dentsply- Petrópolis - Brasil). Os grupos foram aleatoriamente divididos de acordo com o tratamento a ser submetido: imersão no evidenciador de placa (grupo teste) ou imersão em água deionizada (grupo controle). Os grupos teste foram imersos em 10 ml do evidenciador de placa Plax Magic (Colgate-Palmolive, São Bernardo do Campo, SP, Brasil) por 60 s três vezes por dia durante 35 dias, de acordo com a orientações de uso do fabricante. As leituras de cor foram feitas após 24 h da preparação dos espécimes e após 7, 14, 21 e 35 dias de imersão. Os resultados mostraram que o evidenciador de placa Plax Magic promoveu alteração de cor em todos os materiais usados e em todos os tempos de leitura. A alteração de cor foi maior na resina Charisma, seguida da Z250 e EvoluX. Portanto, o evidenciador de placa promove alteração de cor em todos os materiais testados. A resina composta Charisma demonstrou menor estabilidade de cor que as resinas Z250 e EvoluX, que, por sua vez, foi a mais estável. Palavras-chave: Resinas Compostas. Higiene Oral. Cor.

scholarly journals Bond Strength Evaluation of Bulk- fill Composites to Dentin Under Different Surface Treatments

2020 ◽  
pp. 194-207
Author(s):  
Kazım Bas ◽  
Ebru Uslu Cender
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Composite Resin ◽  
Surface Preparation ◽  
Acid Group ◽  
High Viscosity ◽  
Composite Resins ◽  
Control Group ◽  
Significant Difference

Purpose: The aim of this study is to compare the shear bond strength of three high-viscosity bulk-fill composite resins applied as single layers of 4mm thickness and of one microhybrid composite resin applied incrementally with a thickness of 2mm on dentin surfaces prepared with different surface preparation methods. Materials and Methods: A total of 132 caries-free human molars whose dentines had been exposed were used. Three groups, each containing 44 teeth, were formed. The acid group was etched with 32% phosphoric acid; laser group was etched with Er, Cr; YSGG laser and the control group was prepared with silicon carbid paper. Following the application of bonding agent, composite resin materials were applied on teeth using teflon moulds of 4x4mm. The samples were then subjected to shear bond strength tests. Two-way variance analysis and Tukey HSD multiple comparison test were applied on collected data. Results: There was a significant difference between surface treatment methods used regarding shear bond strength (P<0.001). The highest shear bond strength values were detected in the “Acid” group while the lowest shear bond strength values were detected in the “control” group. On the other hand, there was no statistically significant difference among composite resins (P>0.05). Conclusions: Laser etching of the dentin tissues is not as effective as acid etching. Bulk-fill composites had similar effects compared with conventional composite resins with regard to bonding strength. More in vitro studies supported with clinical data are required to investigate the performance of bulk-fill composites and laser.

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