scholarly journals Omega-3 Polyunsaturated Fatty Acids Mitigate Palmitate-Induced Impairments in Skeletal Muscle Cell Viability and Differentiation

2020 ◽  
Vol 11 ◽  
Author(s):  
Bill Tachtsis ◽  
Jamie Whitfield ◽  
John A. Hawley ◽  
Nolan J. Hoffman
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Cell Viability ◽  
Muscle Cell ◽  
Skeletal Muscle Cell ◽  
Omega 3

scholarly journals Omega-3 Polyunsaturated Fatty Acids: Benefits and Endpoints in Sport

Nutrients ◽  
2018 ◽  
Vol 11 (1) ◽  
pp. 46 ◽  
Author(s):  
Maria Gammone ◽  
Graziano Riccioni ◽  
Gaspare Parrinello ◽  
Nicolantonio D’Orazio
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Metabolic Response ◽  
Muscle Metabolism ◽  
Omega 3 ◽  
Cellular Functions ◽  
Inflammatory Conditions ◽  
Inflammatory Processes ◽  
And Performance

The influence of nutrition has the potential to substantially affect physical function and body metabolism. Particular attention has been focused on omega-3 polyunsaturated fatty acids (n-3 PUFAs), which can be found both in terrestrial features and in the marine world. They are responsible for numerous cellular functions, such as signaling, cell membrane fluidity, and structural maintenance. They also regulate the nervous system, blood pressure, hematic clotting, glucose tolerance, and inflammatory processes, which may be useful in all inflammatory conditions. Animal models and cell-based models show that n-3 PUFAs can influence skeletal muscle metabolism. Furthermore, recent human studies demonstrate that they can influence not only the exercise and the metabolic response of skeletal muscle, but also the functional response for a period of exercise training. In addition, their potential anti-inflammatory and antioxidant activity may provide health benefits and performance improvement especially in those who practice physical activity, due to their increased reactive oxygen production. This review highlights the importance of n-3 PUFAs in our diet, which focuses on their potential healthy effects in sport.

scholarly journals Regulation of Skeletal Muscle Satellite Cell Differentiation by Omega-3 Polyunsaturated Fatty Acids: A Critical Review

2021 ◽  
Vol 12 ◽  
Author(s):  
Peter O. Isesele ◽  
Vera C. Mazurak
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Muscle Regeneration ◽  
Critical Review ◽  
Myogenic Differentiation ◽  
Skeletal Muscle Regeneration ◽  
Omega 3 ◽  
Muscle Disorders ◽  
Myogenic Progenitor Cells

Skeletal muscle is composed of multinuclear cells called myofibres, which are formed by the fusion of myoblasts during development. The size of the muscle fiber and mass of skeletal muscle are altered in response to several pathological and physiological conditions. Skeletal muscle regeneration is primarily mediated by muscle stem cells called satellite cells (SCs). In response to injury, these SCs replenish myogenic progenitor cells to form new myofibers to repair damaged muscle. During myogenesis, activated SCs proliferate and differentiate to myoblast and then fuse with one another to form muscle fibers. A reduced number of SCs and an inability to undergo myogenesis may contribute to skeletal muscle disorders such as atrophy, cachexia, and sarcopenia. Myogenic regulatory factors (MRF) are transcription factors that regulate myogenesis and determines whether SCs will be in the quiescent, activated, committed, or differentiated state. Mitochondria oxidative phosphorylation and oxidative stress play a role in the determination of the fate of SCs. The potential activation and function of SCs are also affected by inflammation during skeletal muscle regeneration. Omega-3 polyunsaturated fatty acids (PUFAs) show promise to reduce inflammation, maintain muscle mass during aging, and increase the functional capacity of the muscle. The aim of this critical review is to highlight the role of omega-3 PUFAs on the myogenic differentiation of SCs and pathways affected during the differentiation process, including mitochondrial function and inflammation from the current body of literature.

scholarly journals Fish oil omega-3 fatty acids partially prevent lipid-induced insulin resistance in human skeletal muscle without limiting acylcarnitine accumulation

2014 ◽  
Vol 127 (5) ◽  
pp. 315-322 ◽  
Author(s):  
Francis B. Stephens ◽  
Buddhike Mendis ◽  
Chris E. Shannon ◽  
Scott Cooper ◽  
Catharine A. Ortori ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Fish Oil ◽  
Intravenous Infusion ◽  
Human Skeletal Muscle ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Healthy Males

Intravenous infusion of lipid into healthy males caused insulin resistance. Addition of fish oil omega-3 polyunsaturated fatty acids to the lipid infusion partially prevented the insulin resistance. This effect was not due to differences in muscle acylcarnitine content.

scholarly journals ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Emma Rose Hinkle ◽  
Tasneem Omar Essader ◽  
Gabrielle Marie Gentile ◽  
Jimena Giudice
Keyword(s):  
Skeletal Muscle ◽  
Cell Viability ◽  
Muscle Cell ◽  
Mathematical Knowledge ◽  
Skeletal Muscle Cell ◽  
Biological Features ◽  
Analysis Process ◽  
Actin Protein ◽  
Intuitive Interface ◽  
High Degree

Abstract Background Measuring biological features of skeletal muscle cells is difficult because of their unique morphology and multinucleate nature upon differentiation. Here, we developed a new Fiji macro package called ViaFuse (that stands for viability and fusion) to measure skeletal muscle cell viability and differentiation. To test ViaFuse, we utilized immunofluorescence images of differentiated myotubes where the capping actin protein of muscle z-line subunit beta (CAPZB) was depleted in comparison with control cells. Results We compared the values achieved using the ViaFuse macros first with manual quantification performed by researchers and second with those obtained utilizing the MATLAB muscle-centric software MyoCount. We observed a high degree of correlation between all methods of quantification. Conclusions ViaFuse can detect the borders of myotubes and identify nuclear clumps which have been limitations of previous muscle-centric imaging software. The ViaFuse macros require little computer power or space to run and user inputs to the ViaFuse macros are minimal, thereby automating the analysis process in a quick, easy, and accurate fashion. Additionally, the ViaFuse macros work with Fiji, an existing imaging software widely used by skeletal muscle researchers. Furthermore, ViaFuse is compatible with many computer systems, has a very intuitive interface, and does not require prior complex mathematical knowledge. Therefore, we propose ViaFuse as a robust and meticulous method to quantify skeletal muscle cell viability and differentiation.

scholarly journals The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1276
Author(s):  
Louay Bachnak ◽  
Jean Sparks ◽  
Daniel E. Newmire ◽  
Xavier F. Gonzales ◽  
Felix O. Omoruyi
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Cell Viability ◽  
Cell Lines ◽  
Muscle Cell ◽  
Skeletal Muscle Cell ◽  
Muscle Gene Expression ◽  
Tnf Α ◽  
Muscle Gene

Background: Type 2 diabetes (T2D) is a chronic illness associated with resistance to or defective insulin secretion. This study investigates the effects of thermotherapy on cell viability, gene expression and inflammation in skeletal muscle cell lines. Methods: Healthy and T2D human skeletal muscle cell lines (HSMM and D-HSMM, respectively) were subjected to acute or chronic thermo-therapy (AT or CT, respectively). CT consisted of a 30 min exposure to 40 °C, three times a week for three weeks; AT was a one-time exposure. Results: A significant decrease in D-HSMM cell viability percentage followed AT; however, no significant change occurred in CT. HSMM yielded the highest elevations of genes following CT. In D-HSMM, both treatments yielded gene upregulation. Both treatments significantly down-regulated IL-1β, IL-6, IL-10 and TNF-α in HSMM. AT significantly decreased IL-1β, IL-6 and upregulated IL-10 and TNF-α levels in D-HSMM, while CT yielded a reduction in IL-4, TNF-α and an upregulation of IL-6 and IL-10. Conclusions: An increase in gene expression indicates actin activity and cellular responses, suggesting an increase in transcriptional regulation. The upregulation of IL-6 and IL-10 in D-HSMM negatively correlated with a decrease in TNF-α and IL-1β, indicating improved adverse inflammatory effects associated with the disease.

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