scholarly journals Lab-to-Field Transition of RNA Spray Applications – How Far Are We?

2021 ◽  
Vol 12 ◽  
Author(s):  
Aline Pereira Rank ◽  
Aline Koch

The drastic loss of biodiversity has alarmed the public and raised sociopolitical demand for chemical pesticide-free plant production, which is now treated by governments worldwide as a top priority. Given this global challenge, RNAi-based technologies are rapidly evolving as a promising substitute to conventional chemical pesticides. Primarily, genetically modified (GM) crops expressing double-stranded (ds)RNA-mediating gene silencing of foreign transcripts have been developed. However, since the cultivation of GM RNAi crops is viewed negatively in numerous countries, GM-free exogenous RNA spray applications attract tremendous scientific and political interest. The sudden rise in demand for pesticide alternatives has boosted research on sprayable RNA biopesticides, generating significant technological developments and advancing the potential for field applications in the near future. Here we review the latest advances that could pave the way for a quick lab-to-field transition for RNA sprays, which, as safe, selective, broadly applicable, and cost-effective biopesticides, represent an innovation in sustainable crop production. Given these latest advances, we further discuss technological limitations, knowledge gaps in the research, safety concerns and regulatory requirements that need to be considered and addressed before RNA sprays can become a reliable and realistic agricultural approach.

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1446
Author(s):  
Heather H. Tso ◽  
Leonardo Galindo-González ◽  
Stephen E. Strelkov

Clubroot, caused by Plasmodiophora brassicae, is one of the most detrimental threats to crucifers worldwide and has emerged as an important disease of canola (Brassica napus) in Canada. At present, pathotypes are distinguished phenotypically by their virulence patterns on host differential sets, including the systems of Williams, Somé et al., the European Clubroot Differential set, and most recently the Canadian Clubroot Differential set and the Sinitic Clubroot Differential set. Although these are frequently used because of their simplicity of application, they are time-consuming, labor-intensive, and can lack sensitivity. Early, preventative pathotype detection is imperative to maximize productivity and promote sustainable crop production. The decreased turnaround time and increased sensitivity and specificity of genotypic pathotyping will be valuable for the development of integrated clubroot management plans, and interest in molecular techniques to complement phenotypic methods is increasing. This review provides a synopsis of current and future molecular pathotyping platforms for P. brassicae and aims to provide information on techniques that may be most suitable for the development of rapid, reliable, and cost-effective pathotyping assays.


Energies ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1917
Author(s):  
Benedykt Pepliński ◽  
Wawrzyniec Czubak

In many circles, brown coal continues to be viewed as a cheap source of energy, resulting in numerous investments in new opencast brown coal mines. Such a perception of brown coal energy is only possible if the external costs associated with mining and burning coal are not considered. In past studies, external cost analysis has focused on the external costs of coal burning and associated emissions. This paper focuses on the extraction phase and assesses the external costs to agriculture associated with the resulting depression cone. This paper discusses the difficulties researchers face in estimating agricultural losses resulting from the development of a depression cone due to opencast mineral extraction. In the case of brown coal, the impacts are of a geological, natural-climatic, agricultural-productive, temporal, and spatial nature and result from a multiplicity of interacting factors. Then, a methodology for counting external costs in crop production was proposed. The next section estimates the external costs of crop production arising from the operation of opencast mines in the Konin-Turek brown coal field, which is located in central Poland. The analyses conducted showed a large decrease in grain and potato yields and no effect of the depression cone on sugar beet levels. Including the estimated external costs in the cost of producing electricity from mined brown coal would significantly worsen the profitability of that production.


Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 561
Author(s):  
José A. Fernández-López ◽  
Marta Doval Miñarro ◽  
José M. Angosto ◽  
Javier Fernández-Lledó ◽  
José M. Obón

The sustainable management of biomass is a key global challenge that demands compliance with fundamental requirements of social and environmental responsibility and economic effectiveness. Strategies for the valorization of waste biomass from agrifood industries must be in line with sustainable technological management and eco-industrial approaches. The efficient bioremoval of the pesticides imazalil and thiabendazole from aqueous effluents using waste biomass from typically Mediterranean agrifood industries (citrus waste, artichoke agrowaste and olive mill residue) revealed that these residues may be transformed into cost-effective biosorbents. Agrifood wastes present irregular surfaces, many different sized pores and active functional groups on their surface, and they are abundant in nature. The surface and adsorptive properties of olive mill residue, artichoke agrowaste and citrus waste were characterized with respect to elemental composition, microstructure, crystallinity, pore size, presence of active functional groups, thermal stability, and point of zero charge. Olive mill residue showed the highest values of surface area (Brunauer–Emmett–Teller method), porosity, crystallinity index, and pH of zero point of charge. Olive mill residue showed the highest efficiency with sorption capacities of 9 mg·g−1 for imazalil and 8.6 mg·g−1 for thiabendazole.


HORTUSCOLER ◽  
2020 ◽  
Vol 1 (01) ◽  
pp. 20-26
Author(s):  
Risa Selfiani ◽  
Darmansyah Darmansyah

Eggplant (Solanum melongena L.) is a plant species that is known as the fruit and vegetables grown for use as a food ingredient. These plants belong to one group of plants that produce seeds Eggplant Trunk divided into two kinds, namely the main stem and branching. Eggplant leaf consists of a petiole and the leaf blade. Eggplant flowers are hermaphrodite flowers, or better known as the bisexual flowers, the flowers are one of the stamen to the pistil. Eggplant fruit is the fruit of a true single.. Cultivation of eggplant in the Project of Independent Business is using composting technology bagase. Bagase compost is compost that comes from bagasse or the rest of the sugar mill. Bagase own compost organic matter content of about 90%, N content of 0,3%, 0,02% P2O5, K20 0,14%, Ca 0,06% and 0,04% Mg. The project goal independent business is the use of compost bagase on the cultivation of eggplant to reduce the use of chemical fertilizers and optimize plant production eggplant, farming systems semi-organic environmentally sustainable and analyze the feasibility of cultivation of eggplant with market potential in Payakumbuh and the District Fifty City. Independent business projects implemented during the four months from September to December 2015 and conducted experiments dikebun State Agricultural Polytechnic Payakumbuh with total area of ​​300 m2. Eggplant crop production on the independent business projects with the use of compost bagase treatment that is 575 Kg/300 m2, a profit of Rp. 835.310, the profitability of 92% and RC Ratio of 1,92.


2020 ◽  
pp. 24-27
Author(s):  
Maryna POLENKOVA

The paper defines the structural distribution of sown area of crops. The dynamics of production of cereals and legumes, sugar beets, sunflowers, potatoes, vegetables, fruits and berries. The clustering of regions of Ukraine under similar conditions of development of the enterprises of the agricultural sector specializing in crop production is carried out. According to the results of the analysis, 3 clusters were identified, which gave rise to the possibility of forming (developing) joint effective organizational and economic mechanisms to enhance the development of crop enterprises, favorable for implementation within the cluster. In the course of this study, we focus on the development trends of enterprises specializing in crop products. The lion's share (54.7 %) of sown areas falls on cereals and legumes. Technical crops occupy, which is 32.6 %, and potatoes, vegetables and melons – 6.5 %. It is important to note the long-term positive dynamics of production of cereals and legumes and the growth of production can be traced to all categories of farms – in farms and households. The production of sunflower and vegetable crops was also marked by positive dynamics. The decrease was observed in the production of sugar beets, potatoes, fruit and berry crops. In the context of the study, it is important to identify similar conditions (environment, resources, etc.) for the development of agricultural enterprises between the regions of Ukraine. For this purpose, multifactor statistical analysis (cluster analysis) of key factors of their development was used on the basis of a matrix of 25 selected indicators of development of enterprises specializing in crop production in all regions (600 indicators). To enhance the objectivity of the results, the analysis was conducted in 2010, 2015 and 2019. According to the results of the analysis, taxonomic groups (clusters) were singled out, which made it possible to single out similar regional conditions within each group. Since 2010, there have been significant changes in the regional distribution of Ukraine's agricultural potential. However, during 2015–2019 there is a relative stability in terms of resource provision and use within specific clusters. According to the results of the analysis, three clusters are distinguished: I. Volyn, Zakarpattia, Ivano-Frankivsk, Lviv, Rivne, Chernivtsi, as well as Donetsk and Luhansk regions. II. Dnipropetrovsk, Zhytomyr, Zaporizhia, Kyiv, Mykolaiv, Odesa, Ternopil, Kharkiv, Kherson, Khmelnytsky regions. III. Vinnytsia, Kirovohrad, Poltava, Sumy, Cherkasy and Chernihiv regions.


By continuous and rapid growth in industrialization as well as population, the agricultural lands are also becoming less and less continuously day by day which results in increase in the population of small land holders. In addition to this, because of continuous decreasing production the youth of the villages are also migrating in big cities for the employment, resulting decreasing manpower essential to perform various seedbed operations in the villages. Hence, it is the need of time for small farmers having small agricultural land and having less crop production to introduce the cost-effective farm mechanization so that they can improve production rate. Rotavator is the best option available to achieve this landmark as it is already proved that seedbed prepared by using rotavator gives highest benefit to cost ratio. For this experimental study the whole land of 9 acre area is divided into 18 plots of equal size in area. The combination of method of seedbed preparation and use of fertilizers were the preliminary criteria. The categories of fertilizer according to quantity are discussed earlier which are 50kg, 35kg and 65kg per acre respectively. The quantities of organic fertilizer used are 45kg and 60kg per acre of agricultural land for both seedbeds which are prepared manually and by using rotavator. The highest production of Pigeon Pea was obtained of 814kg was from the plot whose seedbed is prepared by using agricultural machine called rotavator and organic fertilizer; next highest production is obtained of 802 kg from the plot whose seedbed is prepared by using rotavator and the fertilizer used was chemical fertilizer. The minimum production of Pigeon Pea which is 690 kg was obtained in the plot whose seedbed was prepared manually and chemical fertilizer was used.


Author(s):  
Rabia Nazir

Loading of water with multifarious pollutants has dwindled the availability of quality fresh water and put questions on reliability and efficacy of conventional water treatment technologies. Also the quest for developing robust and cost-effective methods with minimum impact on environment had driven the focus of researchers and technologists on new technological developments. Nanotechnology – better referred as Aqua-nanotechnology in this regard provides scientists a new dimension to deal this big problem with small particles having application in 1) water treatment, 2) remediation, and 3) pollution prevention. This chapter will focus on fabrication and use of advance nanomaterials categorized as nanoadsorbents and nanoatalysts for these three main areas. A range of materials exploited in this regard are single and mixed metal oxides and their composites with polymer, clay, carbon based materials etc. while keeping focus on technological developments taken place over the period in regard with treating water and waste water.


2002 ◽  
Vol 92 (12) ◽  
pp. 1356-1362 ◽  
Author(s):  
F. N. Martin ◽  
C. T. Bull

Soil fumigation with methyl bromide plus chloropicrin is used as a preplant treatment to control a broad range of pathogens in high-value annual crop production systems. In California, fumigation is used on approximately 10,125 ha of strawberry production to control pathogens ranging from Verticillium dahliae to root pruning pathogens such as Pythium, Rhizoctonia, or Cylindrocarpon spp. In addition to pathogen control, fumigation also causes an enhanced growth response of the plant and reduces weed pressure. The development of successful, long-term cost effective biocontrol strategies most likely will require the development of an integrated systems approach that incorporates diverse aspects of the crop production system. Although application of single microbial inoculants may provide some level of control for specific production problems, it will be a challenge to provide the broad spectrum of activity needed in production fields.


2020 ◽  
Vol 8 (9) ◽  
pp. 693
Author(s):  
Dracos Vassalos ◽  
Donald Paterson

The traditional risk control option adopted in naval architecture to meet safety-related objectives is by regulations, targeting damage limitation, nominally instigated in the wake of maritime accidents claiming heavy loss of life. These primarily concern the introduction of watertight bulkheads, i.e., permanent (passive) reconfiguration of the internal ship environment to enhance damage stability. This has been the most common measure, manifesting itself in the wake of every serious flooding accident since the beginning, back in the 19th century. However, traditional flooding protection through watertight subdivision, to an extent dictated by IMO regulations, has a physical limit which, if exceeded, a safety plateau is reached. This is currently the case and with damage stability standards progressively increasing, the safety gap between existing and new ships is dangerously widening and with design stability margins progressively eroding, stability management is unsustainable, leading to loss of earnings at best. The need for managing the residual risk through active intervention/protection over the life-cycle of the vessel drives industry to searching and adopting a new normal. This new normal is the innovation being explained in this paper by addressing safety enchantment through a systematic reconfiguration of the ship environment for passive and active protection in flooding accidents. In this respect, the “design-optimal” internal arrangement of a vessel, is adapted and reconfigured, using passive and active containment systems for flooding incidents, in the form of high-expansion foam products. The innovation is briefly explained, claiming transformational reduction in flooding risk in the most cost-effective way available. To support wider understanding and appreciation for the latter, the paper critically reviews the whole evolution of internal ship space reconfiguration, chronologically and systematically, concluding that new technological developments and breakthroughs will bring sustainable changes to the traditional evolutionary maritime safety enhancement.


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