Prevalence, morphological and biochemical study of larval stage Coenurus cerebralis of Taenia multiceps in sheep

Coenurosis caused by Coenurus cerebralis, the larval stage of Taenia multiceps, particularly affects sheep and goats. In this case report, two coenurus cysts were detected under the lumbar spinal cord (outside the CNS) in a goat. We first described cysts in the macro- and micro-morphological examination. The cysts were identified as C. cerebralis on the basis of the arrangement of scolices and the number and size of hooks in the scolices. The morphology of the larval cyst was similar to that of T. multiceps: the scolices had four suckers and a rostellum with a double crown of hooks. The hooks and hooklets were 178 and 132 µm in length, respectively. T. gaigeri may be synonymous with T. multiceps. This may reflect a different host response to the parasite in goats. The difficulty of making a species identification in C. cerebralis or C. gaigeri based on their morphology is discussed.  

Download Full-text

Molecular characterization and phylogenetic analysis of Taenia multiceps from China

Acta Parasitologica ◽

10.1515/ap-2018-0085 ◽

2018 ◽

Vol 63 (4) ◽

pp. 721-727 ◽

Cited By ~ 1

Author(s):

L. Tan ◽

A.B. Wang ◽

S.Q. Zheng ◽

X.L. Zhang ◽

C.J. Huang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Dna Sequences ◽

Hunan Province ◽

Biological Study ◽

Economic Losses ◽

Molecular Characteristics ◽

12S Rrna ◽

Intermediate Hosts ◽

Taenia Multiceps ◽

Coenurus Cerebralis

Abstract Taenia multiceps, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of T.multiceps is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of T.multiceps and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of T.multiceps were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(pcox1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from T.multiceps and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between T.multiceps and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for pcox1, 12S rRNA and ITS, respectively, among T.multiceps and coenurus isolates obtained in this study. In Sichuan province, sequence variations for Coenurus cerebralis isolated from Yaan city were 0–1.4% for pcox1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of T.multiceps isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for pcox1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on pcox1 sequences indicated that all cerebral and noncerebral metacestodes belong to T.multiceps. These results provide reference values for future molecular epidemiological and biological study on T.multiceps in dogs and intermediate hosts.

Download Full-text

Histopathological and Molecular Evaluation of the Experimen-tally Infected Goats by the Larval Forms of Taenia multiceps

Iranian Journal of Parasitology ◽

10.18502/ijpa.v14i1.722 ◽

2019 ◽

Cited By ~ 1

Author(s):

Omidreza AMRABADI ◽

Ahmad ORYAN ◽

Mohammad MOAZENI ◽

Hassan SHARIFIYAZDI ◽

Maryam AKBARI

Keyword(s):

Spinal Cord ◽

Molecular Genetic ◽

Separate Species ◽

Taenia Multiceps ◽

Larval Stages ◽

Morphological Criteria ◽

Coenurus Cerebralis ◽

Brain And Spinal Cord ◽

The Brain ◽

Molecular Evaluation

Background: Introduction of Taenia multiceps and T. gaigeri as two separate species have been recognized mainly on morphological grounds. This experimental study was conducted to test whether cerebral and non-cerebral forms of Coenurus cerebralis belong to one origin or they are originated from two different tape worms. Methods: Two groups of dogs were infected with the cerebral and muscular sources of the coenuri cysts. About two months later the eggs were collected from the fecal samples to be used to experimentally infect other healthy goats. Histopathological and molecular evaluation was conducted in two groups of goats that were challenged with T. multiceps eggs obtained from the infected dogs by brain and muscular sources of coenuri cysts in School of Veterinary Medicine of Shiraz University, Shiraz, Iran in 2015. All aberrant sites of predilection of the metacestode in goats were muscles, heart, diaphragm and lungs. The brain and spinal cord were carefully dissected and examined but the cysts were not found in these locations. In addition, the molecular genetic markers of mitochondrial DNA (CO1 and ND1) were applied to resolve the questionable relationship between T. multiceps and T. gaigeri. Results: The larval stages of T. multiceps in brain and in other aberrant sites, which showed similar morphological criteria, were monophyletic species. Conclusion: Therefore, T. gaigeri must be considered taxonomically invalid.

Download Full-text

Molecular characterization and detection of variants of Taenia multiceps in sheep in Turkey

Parasitology ◽

10.1017/s0031182016001669 ◽

2016 ◽

Vol 144 (2) ◽

pp. 220-225 ◽

Cited By ~ 2

Author(s):

BETUL SONMEZ ◽

ERGUN KOROGLU ◽

SAMI SIMSEK

Keyword(s):

Central Nervous System ◽

Polymerase Chain Reaction ◽

Adult Stage ◽

Infected Sheep ◽

Gene Sequences ◽

Chain Reaction ◽

Taenia Multiceps ◽

Coenurus Cerebralis ◽

The Central Nervous System ◽

Polymerase Chain

SUMMARYTaenia multiceps is a cestode (family Taeniidae) that in its adult stage lives in the small intestine of dogs and other canids. The metacestode, known as Coenurus cerebralis, is usually found in the central nervous system including brain and spinal card in sheep and other ruminants. The presence of cysts typically leads to neurological symptoms that in the majority of cases result in the death of the animal. Coenurosis could cause high losses in sheep farms because the disease commonly affects young animals. A total of 20 C. cerebralis isolates collected from naturally infected sheep in Mardin province of Turkey were characterized through the polymerase chain reaction and sequencing of a fragment of cytochrome c oxidase subunit 1 (CO1) gene. The results showed that the CO1 gene sequences were highly conserved in C. cerebralis isolates. Phylogenetic analysis based on partial CO1 gene sequences revealed that C. cerebralis isolates were composed of three different variants.

Download Full-text

Prevalence and Morphological Characteristics of \textit{Taenia multiceps} Cysts (Coenurus Cerebralis) from Abattoir-Slaughtered and Experimentally Infected Goats

Journal of Neuroparasitology ◽

10.4303/jnp/235532 ◽

2011 ◽

Vol 2 ◽

pp. 1-5 ◽

Cited By ~ 12

Author(s):

Sonia M. S. Afonso ◽

Samson Mukaratirwa ◽

Katarina Hajovska ◽

Bettencourt P. S. Capece ◽

Carles Cristòfol ◽

...

Keyword(s):

Morphological Characteristics ◽

Taenia Multiceps ◽

Coenurus Cerebralis

Download Full-text

Prevalence, predilection sites and pathological findings of Taenia multiceps coenuri in slaughtered goats from south-east Iran

Onderstepoort Journal of Veterinary Research ◽

10.4102/ojvr.v79i1.436 ◽

2012 ◽

Vol 79 (1) ◽

Cited By ~ 8

Author(s):

Reza Kheirandish ◽

Masoud Sami ◽

Shahrzad Azizi ◽

Mohammad Mirzaei

Keyword(s):

Skeletal Muscles ◽

Parietal Lobe ◽

Neuronal Degeneration ◽

Interstitial Fibrosis ◽

Anterior Part ◽

Severe Infection ◽

Abdominal Muscles ◽

Taenia Multiceps ◽

Coenurus Cerebralis ◽

The Brain

Coenurosis is a zoonotic disease in a variety of ruminants caused by the metacestode of Taenia multiceps. The coenuri in the brain and spinal cord of sheep and goats have been identified as Coenurus cerebralis whilst those reported in other tissues have been named Coenurus gaigeri. This study was conducted during the spring and summer of 2011. Out of 25 739 goats inspected in slaughterhouses, 23 carcasses (0.09%) revealed one or multiple visible swellings on the different muscles and visceral organs. The coenuri, of variable sizes, were found mainly in the muscles of the thigh, shoulder and neck, and were less common in the abdominal muscles and subcutaneous tissues. Coenuri were also found in the diaphragm, tongue, intercostal muscles, lung, parotid area and tunica adventitia of the aorta in a goat with severe infection. The brains of slaughtered goats that had coenuri in their skeletal muscles were examined and coenuri were found in two specimens (8.69%). The coenuri were located in the occipital lobe, the anterior part of the right cerebrum and the parietal lobe of the left cerebrum. Histopathologically, coenuri in the brain caused pressure atrophy and liquefactive necrosis in the surrounding tissues, hyperaemia, perivascular cuffing, neuronal degeneration, neuronophagia, satellitosis, diffuse microgliosis and astrocytosis. Coenuri in the skeletal muscles caused degenerative and necrotic changes, hyalinisation and myositis. In the lung, tissues around the coenurus revealed atelectasis and focal interstitial fibrosis. In the present study, concurrent occurrence of coenuri in the central nervous system and skeletal muscles supports the hypothesis that C. cerebralis and C. gaigeri are different names for the metacestodes of the same species of tapeworm.

Download Full-text

Molecular characterization and phylogenetic studies of Echinococcus granulosus and Taenia multiceps coenurus cysts in slaughtered sheep in Saudi Arabia

Open Life Sciences ◽

10.1515/biol-2021-0131 ◽

2021 ◽

Vol 16 (1) ◽

pp. 1252-1260

Author(s):

Jamila S. Al Malki ◽

Nahed Ahmed Hussien

Keyword(s):

Saudi Arabia ◽

Molecular Characterization ◽

Echinococcus Granulosus ◽

Larval Stage ◽

Nucleotide Diversity ◽

Clinical Manifestations ◽

Pcr Amplification ◽

Economic Loss ◽

Taenia Multiceps ◽

Biological Studies

Abstract Taeniids, consisting of two genera Echinococcus and Taenia, are obligatory tapeworms of mammals, and their pathogenicity was due to infection with larval stages. Hydatid (the larval stage of Echinococcus granulosus) and coenurus (the larval stage of Taenia multiceps) cysts are prevalent in domestic, wild ruminants, livestock, swine, and dogs, and accidentally they could also be found in humans. They lead to different clinical manifestations that cause economic loss in livestock and human morbidity. In Saudi Arabia, few studies were performed on hydatid and coenurus cyst genetic variations. The main goal of the present study was to identify E. granulosus and T. multiceps cyst isolates collected from slaughtered Harri sheep in Saudi Arabia by partial sequencing with PCR amplification of the cytochrome C oxidase 1 (COX1) gene. Molecular and phylogenetic evaluation based on COX1 sequences indicated that cyst isolates belong to E. granulosus and T. multiceps, respectively, successfully submitted in NCBI Genbank. Molecular characterization showed a low nucleotide diversity with two submitted isolates of coenurus with related isolates of Genbank. Conversely, E. granulosus isolates showed higher nucleotide diversity. The reported data could serve as a foundation for future molecular epidemiological and biological studies.

Download Full-text

La cénurose des petits ruminants d'Afrique centrale. Les cénuroses africaines, humaines et animales

Revue d’élevage et de médecine vétérinaire des pays tropicaux ◽

10.19182/remvt.7998 ◽

1976 ◽

Vol 29 (4) ◽

pp. 323

Author(s):

M. Graber

Keyword(s):

Afrique De L’Ouest ◽

Taenia Multiceps ◽

Afrique Du Sud ◽

Coenurus Cerebralis ◽

Afrique Centrale ◽

Afrique De L'ouest ◽

Afrique De L’Est

L'auteur signale la présence au Tchad et en République populaire du Congo de Coenurus cerebralis (Batsch, 1786) Rudolphi, 1808 dans le tissu conjonctif sous-cutané et intermusculaire, les séreuses et le cerveau du mouton et de la chèvre. Au total, 7 cas ont été observés sur les 5 000 petits ruminants autopsiés entre 1954 et 1969. Il étudie les cénuroses animales qui, sur le continent africain, sont dues à trois Cestodes parasites de l'intestin des Canidés domestiques et sauvages: Taenia multiceps Leske, 1780, Taenia serialis serialis (Bailliet, 1863) Verster, 1969 n. comb. et Taenia serialis brauni (Setti, 1897) Verster, 1969 n. Comb. Leurs affinités sont précisées. Leurs formes larvaires sont: Coenurus cerebralis des ruminants domestiques et sauvages que l'on trouve non seulement en Afrique du Sud, mais également en Afrique de l'Ouest, de l'Est et du Centre; Coenurus serialis serialis des Léporidés d'Afrique de l'Ouest, du Sud et du Nord-Est; Coenurus serialis brauni des Muridés et de certains singes d'Afrique du Nord, d'Afrique du Sud et d'Afrique de l'Est. La cénurose humaine semble beaucoup plus fréquente que sur les autres continents (77 p. 100 des cas mondiaux connus). Elle revêt deux aspects différents: une forme cérébrale à Coenurus cerebralis en Afrique du Sud et, vraisemblablement, en Afrique centrale; une forme sous-cutanée et oculaire à Coenurus serialis brauni en Afrique de l'Est. L'origine des cas rencontrés en Afrique de l'Ouest n'a pu être établie avec suffisamment de précision

Download Full-text

Cerebral Coenurosis of a Long-Tailed Goral, Naemorhedus caudatus, in Korea

The Korean Journal of Parasitology ◽

10.3347/kjp.2021.59.1.55 ◽

2021 ◽

Vol 59 (1) ◽

pp. 55-59

Author(s):

Sangjin Ahn ◽

Hyeongseok Oh ◽

Soo-Young Choi ◽

Jong-Taek Kim ◽

Hyeon-Cheol Kim

Keyword(s):

Magnetic Resonance Imaging ◽

Computed Tomography ◽

Cerebral Hemisphere ◽

Resonance Imaging ◽

Germinal Layer ◽

Clear Fluid ◽

Taenia Multiceps ◽

Coenurus Cerebralis ◽

Rescue Center ◽

Cerebral Coenurosis

We intended to describe a case of cerebral coenurosis in a long-tailed goral, Naemorhedus caudatus, from Hwacheon-gun, Gangwon-do (Province), in the Korea. The goral, a 10-year-old male, was suffering from neurological symptoms, such as turning the circle to one side without lifting the head straight, and died at 30 days after admission to the wildlife medical rescue center in Chuncheon-si, Gangwon-do. A fluid-filled cyst was detected in the left cerebral hemisphere by computed tomography and magnetic resonance imaging. The cyst removed from the deceased goral was transparent, about 3×3 cm in size, contained a clear fluid and approximately 320 protoscolices invaginating from the internal germinal layer. The protoscolex had 4 suckers and a rostellum with 28 hooklets arranged in 2 rows. By the present study, a case of cerebral coenurosis was first confirmed in a long-tailed goral, N. caudatus, from Gangwon-do, in Korea. The residents frequently exposed in the sylvatic environment should be careful the accidental infections of zoonotic metacestode of Taenia multiceps, Coenurus cerebralis, in Korea.

Download Full-text

First report of coenurosis in sheep in the State of Mato Grosso do Sul, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612010000400016 ◽

2010 ◽

Vol 19 (4) ◽

pp. 265-267 ◽

Cited By ~ 4

Author(s):

Fernando Arévalo Batista ◽

Dietrich Pizzigatti ◽

Charles Ferreira Martins ◽

Marcelo Monteiro Nunes ◽

Tábata Torres Megda ◽

...

Keyword(s):

Right Hemisphere ◽

Pathological Condition ◽

Brain Lesion ◽

The State ◽

Mato Grosso ◽

Taenia Multiceps ◽

First Case ◽

Coenurus Cerebralis ◽

The Right ◽

Mato Grosso Do Sul

This paper reports the first case of coenurosis in the State of Mato Grosso do Sul, Brazil. This disease is caused by the larval stage of the tapeworm Taenia multiceps (Leske, 1780). The animal in which the disease was diagnosed was an 18-month-old ewe from an endemic area of Southern Brazil as an imported animal among a group of 30 sheep. The clinic-pathological condition was that commonly found in herbivores affected by the disease, especially sheep. Apathy, nystagmus, intermittent blindness, circling and pressing head against obstacles were the neurological signs reported. The necropsy showed that a brain lesion in the subcortex of the right hemisphere was a bladder-like cyst measuring 4 cm in diameter filled with a translucent fluid with a large number of white spherules (protoscolices) floating. In addition to the identification of the Coenurus cerebralis protoscolices, the brain tissue lesion was histopathologically described.

Download Full-text