scholarly journals Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1160
Author(s):  
Senlin Xiao ◽  
Tianjun Xu ◽  
Yuandong Wang ◽  
Jinfeng Xing ◽  
Ronghuan Wang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Germination Rate ◽  
Growth Potential ◽  
Breeding Strategies ◽  
Maize Seed ◽  
Peroxide Content ◽  
Similar Content ◽  
The Difference ◽  
Ascorbate Biosynthesis

The impacts of low temperature occasionally encountered at higher latitude regions on maize seed germination present significant threats to yield and cultivation. Exploring the association of antioxidant system with low temperature (LT) germination could support the breeding strategies for better responding to LT disturbance. In this study, we have examined the germination rate and growth potential of a set of elite maize inbred accessions under LT and normal temperature (NT) conditions in the field. These accessions were found to have variable germination rate and growth potential when grown at LT, whereas the difference is not significant under NT. Physiological study revealed lower hydrogen peroxide content in LT tolerant accessions when compared with sensitive ones. LT-tolerant and LT-sensitive lines maintained similar content of ascorbate (AsA) and glutathione (GSH), whereas the reduced substrate content of which were significantly higher in LT-tolerant accessions. Consistently, activities of ascorbate peroxidase and dehydroascorbate reductase, the enzyme components that responsible for the AsA-GSH recycling, were much higher in LT-tolerant lines. Transcription profile revealed the increased expression of ZmVTC2 gene in LT-tolerant inbred line, which was rate limited step in AsA biosynthesis. These data indicates that the coordinated improvement of AsA biosynthesis and AsA-GSH recycling increase the pool size of the total antioxidants, which ameliorate LT-induced oxidative stress during maize seed germination.

Photosynthesis and antioxidant metabolism modulate the low-temperature resistance of seed germination in maize

2021 ◽  
Author(s):  
Aiju Meng ◽  
Daxing Wen ◽  
Chunqing Zhang
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Temperature Stress ◽  
Seedling Growth ◽  
Low Temperature Stress ◽  
Maize Seed ◽  
Down Regulation ◽  
Antioxidant Metabolism ◽  
Temperature Resistance ◽  
Low Temperature Resistance

Spring maize is usually subjected to low-temperature stress during seed germination, which retards seedling growth even if under a suitable temperature. However, the mechanism underlying maize seed germination under low-temperature stress modulating seedling growth after being transferred to normal temperature is still ambiguous. In this study, we used two maize inbred lines with different low-temperature resistance (SM and RM) to investigate the mechanism. The results showed that the SM line had higher lipid peroxidation and lower total antioxidant capacity and germination percentage than the RM line under low-temperature stress, which indicated that the SM line was more vulnerable to low-temperature stress. Further transcriptome analysis revealed that seed germination under low-temperature stress caused down-regulation of photosynthesis related gene ontology (GO) terms in two lines. Moreover, the SM line displayed down-regulation of ribosome and superoxide dismutase (SOD) related genes, whereas genes involved in SOD and vitamin B6 were up-regulated in the RM line. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that photosynthesis and antioxidant metabolism related pathways played important roles in seed germination in response to low-temperature stress, and the photosynthetic system displayed a higher damage degree in the SM line. Both qRT-PCR and physiological characteristics experiments showed similar results with transcriptome data. Taken together, we propose a model for maize seed germination in response to low-temperature stress.

scholarly journals Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions

Genome ◽  
2016 ◽  
Vol 59 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Hari D. Upadhyaya ◽  
Yi-Hong Wang ◽  
Dintyala V.S.S.R. Sastry ◽  
Sangam L. Dwivedi ◽  
P.V. Vara Prasad ◽  
...  
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Cold Tolerance ◽  
Germination Rate ◽  
Strong Association ◽  
Marker Locus ◽  
Seedling Vigor ◽  
Mini Core Collection ◽  
Genome Wide ◽  
Soil Temperatures

Sorghum is one of the world’s most important food, feed, and fiber crops as well as a potential feedstock for lignocellulosic bioenergy. Early-season planting extends sorghum’s growing season and increases yield in temperate regions. However, sorghum’s sensitivity to low soil temperatures adversely impacts seed germination. In this study, we evaluated the 242 accessions of the ICRISAT sorghum mini core collection for seed germination and seedling vigor at 12 °C as a measure of cold tolerance. Genome-wide association analysis was performed with approximately 162 177 single nucleotide polymorphism markers. Only one marker locus (Locus 7-2) was significantly associated with low-temperature germination and none with vigor. The linkage of Locus 7-2 to low-temperature germination was supported by four lines of evidence: strong association in three independent experiments, co-localization with previously mapped cold tolerance quantitative trait loci (QTL) in sorghum, a candidate gene that increases cold tolerance and germination rate when its wheat homolog is overexpressed in tobacco, and its syntenic region in rice co-localized with two cold tolerance QTL in rice. This locus may be useful in developing tools for molecular breeding of sorghums with improved low-temperature germinability.

scholarly journals Improving of true shallot seeds germination by the application of plant growth regulators and osmoconditioning treatment

2022 ◽  
Vol 951 (1) ◽  
pp. 012065
Author(s):  
K A Tanjung ◽  
L A M Siregar ◽  
R I M Damanik
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Root Length ◽  
Germination Rate ◽  
Block Design ◽  
Dry Weight ◽  
Peg 6000 ◽  
The Difference

Abstract This study aims to determine the effect of the application of plant growth regulators and osmoconditioning treatment to improve the germination of true shallot seeds. This research was conducted in Asam Kumbang, Medan Selayang, Medan, Indonesia. The research method was a Randomize Block Design with 2 factors, the first factor is Plant Growth Regulators (Z) with 6 levels, namely Z0 (Without PGRs Application), Z1 (Gibberellin 500 ppm), Z2 (Putrescine 15 ppm), Z3 (Putrescine 20 ppm), Z4 (Putrescine 15 ppm + Gibberellin 500 ppm), Z5 (Putrescine 20 ppm + Gibberellin 500 ppm). The second factor was the osmoconditioning treatment with Polyethylene Glycol (PEG) 6000 (O) with 4 levels, namely O0 (Without Osmoconditioning Treatment), O1 (PEG 6000 3%), O2 (PEG 6000 4%), O3 (PEG 6000 5%). Parameters observed were germination rate, germination rate index, percentage of germination, germination ability, simultaneous growth of seeds, seedling length, root length, seedling dry weight, and catalase activity test. The results of this study were: application of plant growth regulators could improve true shallot seed germination, indicated by the observed values of all parameters which were significantly different from those of the control (without PGRs application). The plant growth regulator that produced the best increase in germination was Gibberellins 500 ppm, although the difference in effect with other PGRs was not significantly different. Meanwhile, the osmoconditioning treatment with PEG 6000 was also able to improve the germination of true shallot seeds as indicated by an increase in most of the observed parameters, but in the root length parameter it was seen that the tendency of PEG 6000 3% always gave the highest value but gave the lowest value for this parameter. The best concentration of PEG 6000 in the osmoconditioning treatment to improve true shallot seed germination was 3%.

scholarly journals PEMATAHAN DORMANSI BENIH KELAPA SAWIT (Elaeis guinensis Jacq.) MENGGUNAKAN KALIUM NITRAT (KNO3) DAN AIR KELAPA

AgriPeat ◽  
2021 ◽  
Vol 22 (01) ◽  
pp. 1-10
Author(s):  
Kaliamsyah Sinaga ◽  
Chotimah Hastin Ernawati Nur CC ◽  
Yusurum Jagau
Keyword(s):  
Seed Germination ◽  
Oil Palm ◽  
Germination Rate ◽  
Water Immersion ◽  
Water Concentration ◽  
Maximum Growth ◽  
Growth Potential ◽  
Coconut Water ◽  
Growth Speed ◽  
Randomized Design

The aim of this study were, 1) to find out and study the effect of KNO3 immersion treatment on oil palm seed germination; 2) to find out and study the effect of coconut water immersion treatment on oil palm seed germination; 3) to find out and study the interactions that occur between KNO3 immersion treatment and coconut water to oil palm seed germination. This research was conducted in July 2019 until August 2019 in the Laboratory of the Department of Agriculture Cultivation, Faculty of Agriculture, Palangka Raya University using a Completely Randomized Design with 2 factors. The first factor is the concentration of KNO3: K0 (0%); K1 (0.2%); K2 (0.4%); K3 (0.6%) and the second factor is coconut water concentration, B0 (0%); B1 (60%); B2 (80%); B3 (100%). The parameters observed were first count germination, maximum growth potential, germination power, growth speed, radicle length, plumular length and dormancy intensity. The results showed that the treatment of KNO3 concentration significantly affected first count germination, maximum growth potential, germination rate, growth speed and dormancy intensity. Treatment of coconut water concentration significantly affected first count germination, maximum growth potential, germination rate, growth speed and dormancy intensity. The interaction between the treatment of KNO3 concentration and coconut water concentration had no significant effect on all observed parameters

scholarly journals Low-temperature Germination of Melons Is Affected by Seedcoat Characteristics and Embryo Genotype

HortScience ◽  
2009 ◽  
Vol 44 (5) ◽  
pp. 1412-1414 ◽  
Author(s):  
Menahem Edelstein ◽  
Haim Nerson
Keyword(s):  
Low Temperature ◽  
Germination Rate ◽  
Germination Percentage ◽  
The Other ◽  
Pressure Force ◽  
Cold Tolerant ◽  
Cold Sensitive ◽  
The Difference ◽  
Melon Seeds ◽  
Dominant Genes

Germination percentage and germination rate were examined in two melon accessions differing in their ability to germinate under low temperature and in their reciprocal F1, F2, and BC1 progeny. The seedcoat structure, pressure force required for seedcoat splitting, and the response to hilum sealing of the reciprocal F1s were examined as well. The purpose of this study was to elucidate the effects of embryonic genotype and seedcoat characteristics on the ability of melon seeds to germinate under low temperature. The results of the study point out that both components are strongly involved in low-temperature germination. The cold tolerant parent, ‘Persia 202’ (P202), germinated greater than 90% at 15 °C, whereas the cold-sensitive parent, ‘Noy Yizre'el’ (NY), did not germinate at all. The P202 × NY F1 and the reciprocal F2 germinated 80% to 90%, whereas the NY × P202 F1 germination percentage was only 71%. Backcrosses of the reciprocal F1 to the parents revealed that if the cross was to the tolerant one, the seeds germinated greater than 90%, but if the F1s were backcrossed to the sensitive parent, the seeds had only 56% to 60% germination. Data collected suggest that several dominant genes are carried by P202 for low-temperature germination. On the other hand, the difference in germination percentages between the reciprocal F1 demonstrates that the different seedcoats also play a role.

scholarly journals 261 Effect of SMP and Brushing on Gourd Seed Germination at Different Temperatures

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 487B-487
Author(s):  
Hae-Jeen Bang ◽  
Soo-Jung Hwang ◽  
Hyun-Sook Ham ◽  
Jung-Myung Lee
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Solid Matrix ◽  
Seed Processing ◽  
Long Term Storage ◽  
Combined Use ◽  
Different Temperatures ◽  
Treated Seed ◽  
The Difference

The effectiveness of solid matrix priming (SMP) and seed brushing was further evaluated by using an thermo-gradient table (Seed Processing, Holland) set at 10 different temperatures from 12 to 30 °C. Intact or brushed seeds of gourd (Lagenaria siceraria) were primed with Micorocel E (Celite Corp.) at 25 °C for 3 days in the mixture of 10 seed: 1 Microcel E: 3 water, by weight, and the primed seeds were dried again for long-term storage. SMP treatment significantly increased earlier seed germination at all temperatures. However, the difference in seed germination rate between intact and SMP-treated seeds was most pronounced at somewhat lower temperatures of 18-22 °C. SMP-treated seed showed about 20% final germination rate at 12 °C, whereas intact seeds did not germinate at all. Seed brushing treatment itself did not influenced the germination rate. However, brushing treatment before SMP treatment significantly increased the SMP effect. Combined use of chemicals in solution further increased the early germination. Details of various seed treatment methods will be presented.

scholarly journals Expression of genes related to tolerance to low temperature for maize seed germination

2015 ◽  
Vol 14 (1) ◽  
pp. 2674-2690 ◽  
Author(s):  
I.C. Silva-Neta ◽  
E.V. Pinho ◽  
A.D. Veiga ◽  
R.G. Pìnho ◽  
R.M. Guimarães ◽  
...  
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Maize Seed ◽  
Expression Of Genes

scholarly journals Nitric Oxide Pre-Treatment Advances Seed Germination and Alleviates Copper-Induced Photosynthetic Inhibition in Indian Mustard

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 776
Author(s):  
Bilal A. Rather ◽  
Iqbal R. Mir ◽  
Asim Masood ◽  
Naser A. Anjum ◽  
Nafees A. Khan
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Seed Germination ◽  
Antioxidant Defense ◽  
Germination Rate ◽  
Indian Mustard ◽  
Antioxidant Defense System ◽  
Growth Potential ◽  
Defense System ◽  
Pre Treatment

This investigation tested the efficiency of nitric oxide (NO) in alleviation of Cu-induced adverse impacts on seed germination and photosynthesis in Indian mustard (Brassica juncea L.). Pre-treatment of B. juncea seeds with sodium nitroprusside (SNP; NO donor) significantly improved the seed germination rate and also alleviated Cu-accrued oxidative stress. However, in the absence of NO, Cu caused a higher reduction in seed germination rate. The presence of NO strengthened the antioxidant defense system (glutathione reductase, ascorbate peroxidase, and superoxide dismutase) and thereby sustained the lower lipid peroxidation, reduced H2O2 content, and thiobarbituric acid reactive substances in Cu-exposed seeds. NO pre-treated seeds also retained a higher amylase activity and exhibited an improved seed germination rate. This effect of NO under Cu stress was also seen in plants originated from the NO pre-treated seeds, where the role of NO pre-treatment was reflected in the improved photosynthetic potential of B. juncea. Overall, NO pre-treatment not only improved the germination rate in seeds but also carried its effects in the grown seedlings evidenced as improved photosynthesis and growth. Potential mechanisms involved in the action of NO pre-treatment included NO-mediated significant strengthening of the antioxidant defense system and decreases in Cu-caused oxidative stress parameters.

Sign in / Sign up

Export Citation Format

Share Document