PEMATAHAN DORMANSI BENIH KELAPA SAWIT (Elaeis guinensis Jacq.) MENGGUNAKAN KALIUM NITRAT (KNO3) DAN AIR KELAPA

Redpalm isornamentplantwhichpotentiallytobecultured.Thescarcityofredpalmmadeithashighmarket value and need to be preserved. However, the germination growth of red palm is slower than other kinds of palm, so the best way to germinate the red palm seed is needed. The aim of this research is to determine the effect of apliccation some young coconut water concentration and kascing fertilizer to red palm seed germination. The research used factorial completely randomized design with 5 replication, the first factor is young coconut water concentration (0%, 25%, 50%, 75%, and 100%) and the second factor is germination media (steril sand and kascing fertilizer). Variables measured were live germination percentage, speed germination rate, radicula length, and plumula height. Results of this study showed that the best germination media is steril sand and 0% concentration of young coconut water.

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Harvesting Criteria and Drying Methods to Improve the Quality of Foxtail Millet Seeds (Setaria italica L. Beauv.)

Journal of Tropical Crop Science ◽

10.29244/jtcs.8.03.168-176 ◽

2021 ◽

Vol 8 (03) ◽

pp. 168-176

Author(s):

Putri Aulia Lainufar ◽

Abdul Qadir ◽

M. Rahmad Suhartanto ◽

Sintho Wahyuning Ardie

Keyword(s):

Seed Quality ◽

Germination Rate ◽

Foxtail Millet ◽

Seed Viability ◽

Dry Weight ◽

Maximum Growth ◽

Growth Potential ◽

Growth Speed ◽

Vigor Index

Foxtail millet is annual grasses with grains that are smaller than those of sorghum, rice, and wheat, and is considered one of the minor economic crops but with nutritional values similar to other major food crops. The development of millet as major cereal crop is considered challenging due to the low quality of its seeds, and studies have been conducted to improve millet’s seed quality. We conducted this study to determine the harvesting criteria of foxtail millet seeds based on the change in color of the panicles. We also wanted to determine the drying treatment, and evaluate the relationship between the position of the seed on the panicles and the seed viability and vigor. The first experiment was arranged based on a completely randomized design with panicle color as the first factor (green, yellow 75%, and brown) and drying time as the second factor (0 hours, 24 hours, 48 hours, 72 hours). The second experiment was arranged in a completely randomized design with seed position as the main factor (base, middle, tip). The study was conducted on two genotypes of millet, i.e. “BOTOK 4” and “BOTOK 10”. The highest seed quality of “BOTOK 4” and “BOTOK 10” genotypes were obtained when the panicles were brown and dried for 72 hours; the seed chlorophyll content was the lowest and water content was 8.88%, with the highest viability and vigor, i.e., germination rate of 88.3%-90%, maximum growth potential of 92%-95.3%, normal sprout dry weight 596-620 mg, vigor index of 33.6% - 21.6%, and growth rate of 18.2%-17.1% etmal-1. The highest seed viability and vigor in “BOTOK 4” genotypes were obtained from the base position, i.e., 78.7% germination rate, maximum growth potential of 83.7%, vigor index of 56.5%, growth speed of 19.6 etmal-1, normal sprout dry weight of 48 mg, radicle length of 3.3 cm) and “BOTOK 10” genotypes from the middle position with 91.5% germination, maximum growth potential of 97.2%, vigor index of 21.7%, growth speed of 17.0% etmal-1, and normal sprout dry weight 61 mg.

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Efektivitas Invigorasi Benih Cabai (Capsicum annuum L) Kadaluarsa Menggunakan Rizobakteri Pemacu Pertumbuhan Tanaman

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v5i2.15034 ◽

2020 ◽

Vol 5 (2) ◽

pp. 121-129

Author(s):

Hafiz Juanda ◽

Hasanuddin Hasanuddin ◽

Syamsuddin Syamsuddin

Keyword(s):

Capsicum Annuum ◽

Bacillus Megaterium ◽

Maximum Growth ◽

Growth Potential ◽

Growth Speed ◽

Department Of Agriculture ◽

Capsicum Annuum L ◽

Randomized Design ◽

Two Factors ◽

Banda Aceh

Abstrak. Penelitian ini bertujuan untuk mengetahui Efektivitas Invigorasi Benih Cabai (Capsicum annuum L.) Kadaluarsa Menggunakan Rizobakteri Pemacu Pertumbuhan Tanaman. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Program Studi Agroteknologi Fakultas Pertanian Universitas Syiah Kuala Darussalam Banda Aceh. Penelitian dilaksanakan mulai pada bulan Januari 2020. Pada penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial 2X9 dengan dua faktor yang diteliti. Faktor pertama varietas (V) yang terdiri dari 2 taraf yaitu: V1 (Lado F1) dan V2 (Krida F1). Faktor kedua jenis Rizobakteri (R) yang terdiri dari 9 taraf yaitu R0 : Kontrol, R1 : Bacillus larvae, R2 : Pseudomonas capasia, R3 : Azotobacter sp. R4 : Acitinobacillus suis, R5 : Bacillus polymixa, R6 : Bacillus megaterium, R7 : Bacillus stearothermophillus, R8 : Bacillus lichiniformis. Dengan demikian terdapat 18 kombinasi perlakuan. Masing - masing kombinasi perlakuan diulang sebanyak tiga kali, dengan demikian terdapat 54 satuan percobaan. Peubah yang diamati adalah potensi tumbuh (%), daya berkecambah (%), keserampakan tumbuh (%), indeks vigor (%), kecepatan tumbuh relatif (%) dan T50 (%). Hasil penelitian yang dilakukan menunjukkan bahwa interaksi antara rizobakteri jenis Azotobacter dengan varietas Lado F1 efektif dalam meningkatkan potensi tumbuh maksimum dan daya berkecambah. Interaksi antara rizobakteri jenis Bacillus polymixa dengan varietas Krida F1 efektif dalam meningkatkan potensi tumbuh maksimum dan daya berkecamabah.The Effectiveness of Invigorating Chili Seeds (Capsicum annuum L.) Expired Using Rhizobacteria Booster Plant GrowthAbstract. This study aims to determine the effectiveness of the Invigoration of Expired Chilli (Capsicum Annum L.) Seeds Using Plant Growth Booster Rizobacteria. This research was carried out in the Seed Science and Technology Laboratory of the Department of Agriculture, Faculty of Agriculture, Syiah Kuala University, Banda Aceh. The study was conducted starting in January 2020. In this study used a 2X9 factorial Complete Randomized Design (CRD) with two factors examined. The first factor is variety (V) which consists of 2 levels, namely: V1 (Lado F1) and V2 (Krida F1). The second factor is the type of Rizobacteria (R) which consists of 9 levels, namely R0 : Control, R1 : Bacillus larvae, R2 : Pseudomonas capasia, R3 : Azotobacter sp, R4 : Acitinobacillus suis, R5 : Bacillus polymixa, R6: Bacillus megaterium, R7: Bacillus stearotherinoph, R8 : Bacillus lichiniformis. Thus there are 18 treatment combinations. Each treatment combination was repeated three times, thus there were 54 experimental units. The observed variables were growth potential (%), germination (%), growth uniformity (%), vigor index (%), relative growth speed (%), and T50 (%). The results of the research showed that the interaction between Azotobacter rizobacteria and Lado F1 varieties was effective in increasing maximum growth potential and germination. The interaction between Bacillus polymixa rizobacteria and Krida F1 variety is effective in increasing the maximum growth potential and the ability to contaminate

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Penggunaan kalium nitrat dalam pematahan dormansi fisiologis setelah pematangan pada beberapa galur padi mutan organik spesifik lokal Aceh

Kultivasi ◽

10.24198/kultivasi.v19i1.25468 ◽

2020 ◽

Vol 19 (1) ◽

pp. 1061

Author(s):

Halimursyadah Halimursyadah ◽

Syamsuddin Syamsuddin ◽

Hasanuddin Hasanuddin ◽

Efendi Efendi ◽

Najwa Anjani

Keyword(s):

Significant Interaction ◽

Dry Weight ◽

Maximum Growth ◽

Growth Potential ◽

Growth Speed ◽

Relative Growth ◽

Significance Level ◽

Randomized Design ◽

Two Factors ◽

Vigour Index

Sari. Induksi mutasi radiasi merupakan metode efektif untuk meningkatkan keragaman tanaman. Kajian akan sifat-sifat yang dibawa oleh generasi galur padi hasil mutasi penting untuk dipelajari. Penelitian ini bertujuan untuk mengetahui pengaruh radiasi sinar gamma terhadap sifat dormansi fisiologis after ripening pada galur padi mutan organik dan mengetahui keefektifan penggunaan konsentrasi KNO3 terhadap upaya pematahan dormansinya. Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih, Fakultas Pertanian, Universitas Syiah Kuala. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor serta menggunakan uji lanjut Tukey pada taraf nyata 5%. Faktor pertama adalah galur padi mutan organik terdiri 5 taraf yaitu G0 = tanpa radiasi (Sanbei Simeleu) sebagai pembanding, G1= Sultan Unsrat, G2= 39e, G3= 75d, G4=57e. Faktor kedua adalah konsentrasi KNO3 terdiri 3 taraf yaitu K0 = 0%, K1 = 1%, dan K2 = 2%. Parameter yang diamati adalah potensi tumbuh maksimum, daya berkecambah, indeks vigor, keserempakan tumbuh, kecepatan tumbuh relatif, berat kering kecambah normal, dan persistensi dormansi. Hasil penelitian menunjukkan bahwa galur padi mutan organik berpengaruh sangat nyata terhadap berat kering kecambah normal dan berpengaruh nyata terhadap potensi tumbuh maksimum. Konsentrasi KNO3 berpengaruh sangat nyata terhadap daya berkecambah dan berat kering kecambah normal, serta berpengaruh nyata terhadap potensi tumbuh maksimum dan kecepatan tumbuh relatif. Terdapat interaksi sangat nyata antara galur padi mutan organik dan konsentrasi KNO3 terhadap berat kering kecambah normal, dan interaksi nyata terhadappotensi tumbuh maksimum, dan daya berkecambah. Kombinasi terbaik dijumpai pada galur padi mutan organik 57e dan konsentrasi KNO3 2%.Kata kunci: Setelah pematangan ∙ Dormansi ∙ Galur mutan ∙ KNO3 ∙ Padi Abstract. This study aims to determine the effect of gamma radiation on the physiological dormancy after-ripening of organic mutant rice lines and the effectiveness using KNO3 concentrations on efforts to break dormancy. The study was conducted at the Seed Science and Technology Laboratory, Syiah Kuala University. This research used a completely randomized design (CRD) with two factors and Tukey test at significance level of 5%. The first factor was the organic mutant rice lines that consisted of 5 levels. There were G0 = without radiation (Sanbei Simeleu) as a comparison, G1 = Sultan Unsrat, G2 = 39e, G3 = 75d, and G4 = 57e. The second factor was KNO3 concentration, that consisted of 3 levels. There were K0 = 0%, K1 = 1%, and K2 = 2%. The observed parameters were dormancy persistence, maximum growth potential, germination, vigour index, the simultaneity of growth, relative growth speed, and normal germination dry weight,. The results showed that the organic mutant rice lines had a very significant effect on the dry weight of normal sprouts and had a significant effect on maximum growth potential. KNO3 concentration has a very significant effect on germination and dry weight of normal sprouts and has a significant effect on the maximum growth potential and relative growth speed. There was a highly significant interaction between organic mutant rice lines and KNO3 concentration on the normal dry weight of the sprouts, and significant interaction with maximum growth potential and germination. The best interaction was found in organic mutant rice lines 57e and KNO3 concentration of 2%.Keywords: After ripening ∙ Dormancy ∙ KNO3 ∙ Mutantline ∙ Rice

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Physiological and biochemical changes in cocoa seed (Theobroma cacao L.) caused by desiccation Perubahan fisiologi dan biokimia benih kakao (Theobroma cacao L.) akibat desikasi

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v68i1.135 ◽

2016 ◽

Vol 68 (1) ◽

Author(s):

Nurita TORUAN-MATHIUS ◽

. RACHMAWATI-HASID ◽

. NURHAIMI-HARIS ◽

Tolhas HUTABARAT

Keyword(s):

Seed Germination ◽

Water Content ◽

Theobroma Cacao ◽

Germination Rate ◽

Dry Weight ◽

Maximum Growth ◽

Growth Potential ◽

Normal Seedling ◽

Theobroma Cacao L ◽

Physiological And Biochemical

Ringkasan Benih kakao tergolong rekalsitran, benihnya sensitif terhadap desikasi dan apabila disimpan pada kondisi yang menyebabkan kehilangan air, benih akan kehilangan viabilitasnya. Viabilitas benih kakao hanya dapat dipertahankan beberapa hari saja dalam keadaan terbuka pada suhu kamar. Hal ini merupakan kendala dalam penyimpanan dan pengiriman benih kakao. Tujuan penelitian ini adalah untuk menetapkan pengaruh desikasi terhadap karakter fisiologis dan biokimia benih kakao. Benih ICS 60 (kakao lindak) dan DR2 (kakao mulia) diletakkan dalam cawan Petri kemudian disimpan pada suhu 25oC dan Rh 55-75% selama empat hari. Percobaan dilakukan dengan rancangan petak terpisah, petak utama adalah kandungan air awal dan kritikal. Sebagai anak petak adalah jenis kakao, masing-masing diulang empat kali. Peubah fisiologis yang diukur adalah viabilitas benih mencakup kandungan air benih, potensi tumbuh maksimum, daya berkecambah, kecepatan tumbuh, bobot kering kecambah normal, dan laju pertumbuhan kecambah normal. Di samping itu juga dilakukan pengamatan pola pita protein benih yang dianalisis dengan SDS-PAGE. Kandungan asam absisik (ABA) dan gula stahiosa, raftnosa, glukosa, fruktosa, arabinosa, silosa, serta sukrosa dalam benih yang ditetapkan dengan HPLC Integritas membran benih ditetapkan berdasarkan daya hantar listrik air perendaman benih yang diukur dengan konduktometer. Hasil yang diperoleh menunjukkan bahwa adanya interaksi yang nyata antara desikasi dengan seluruh tolok ukur fisiologis. Desikasi menyebabkan penurunan daya berkecambah, bobot kering dan laju pertumbuhan kecambah normal, potensi tumbuh maksimum dan kecepatan tumbuh. Sedang untuk, kandungan ABA, sukrosa, arabinosa dan rafinosa mengalami peningkatan. Di samping itu desikasi menyebabkan dibentuknya protein baru dengan BM 32,5; 47,0 dan 51,0 kDa (DR2); 47,0 dan 51,0 kD (ICS 60). Beberapa protein yang hilang oleh pengaruh desikasi yaitu dengan BM37, 0 (DR2), 19, 0 dan 37, 0 kD (ICS60). Benih ICS60 lebih tahan terhadap desikasi dibandingkan dengan benih DR2. Summary Seed of cocoa is recalcitrant and sensitive to desiccation. In open condition at room temperature, the viability of cocoa seed ultimately lost for several days. These characters are a problem for seed storage and delivery. The objectives of this study are to investigate the effect of desiccation on physiological and biochemical characters of cocoa seed. Seeds of ICS 60 (bulk cocoa) and DR2 (fine cocoa) were placed on Petri dishes and stored at 25oC, Rh 55-75% for four days (critical water content). The experiment was conducted with split plot analysis, (1) The main plot was the storage condition initial and critical seeds water content. (2) The sub plot was the variety of cocoa, with four replications of each treatment. The effect of desiccation on seeds viability was tested, based on seed water content, maximum growth potential, seed germination, germination rate, dry weight of normal seedling, and seedling growth rate. Besides, the changes of seed proteins band pattern were also analysed by SDSPAGE. Abscisic acid, stachyose, raffnose, fructose, arabinose, xyllose, and sucrose seed content were determined by HPLC. The integrity of seed membrane based on the leakage of electrolytes from seeds was measured with a CM 100 multicell conductivity meter. The results showed that there is an interaction with highly significant correlation between desiccation and all of the physiological and biochemical parameters. Desiccation caused the decrease of seed germination, dry weight and growth rate'of normal seedling, maximum growth potential, and germination rate and while the leakage of electrolytes, ABA, sucrose, arabinose and raffinose increased. Besides, desiccation was also caused the formation of new proteins with MW 32.5, 47,0 and 51,0 kDa (DR2); 47,0 and 51,0 kD ICS 60) . On the other hand, several protein were disappeared i.e. MW 37,0 (DR2), 19,0 and 37,0 kD (ICS60). Seeds of ICS 60 are more tolerant to desiccation than seeds of DR2.

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Physiological and biochemical changes in cocoa seed (Theobroma cacao L.) caused by desiccation Perubahan fisiologi dan biokimia benih kakao (Theobroma cacao L.) akibat desikasi

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v68i1.135 ◽

2016 ◽

Vol 68 (1) ◽

Author(s):

Nurita TORUAN-MATHIUS ◽

. RACHMAWATI-HASID ◽

. NURHAIMI-HARIS ◽

Tolhas HUTABARAT

Keyword(s):

Seed Germination ◽

Water Content ◽

Theobroma Cacao ◽

Germination Rate ◽

Dry Weight ◽

Maximum Growth ◽

Growth Potential ◽

Normal Seedling ◽

Theobroma Cacao L ◽

Physiological And Biochemical

Ringkasan Benih kakao tergolong rekalsitran, benihnya sensitif terhadap desikasi dan apabila disimpan pada kondisi yang menyebabkan kehilangan air, benih akan kehilangan viabilitasnya. Viabilitas benih kakao hanya dapat dipertahankan beberapa hari saja dalam keadaan terbuka pada suhu kamar. Hal ini merupakan kendala dalam penyimpanan dan pengiriman benih kakao. Tujuan penelitian ini adalah untuk menetapkan pengaruh desikasi terhadap karakter fisiologis dan biokimia benih kakao. Benih ICS 60 (kakao lindak) dan DR2 (kakao mulia) diletakkan dalam cawan Petri kemudian disimpan pada suhu 25oC dan Rh 55-75% selama empat hari. Percobaan dilakukan dengan rancangan petak terpisah, petak utama adalah kandungan air awal dan kritikal. Sebagai anak petak adalah jenis kakao, masing-masing diulang empat kali. Peubah fisiologis yang diukur adalah viabilitas benih mencakup kandungan air benih, potensi tumbuh maksimum, daya berkecambah, kecepatan tumbuh, bobot kering kecambah normal, dan laju pertumbuhan kecambah normal. Di samping itu juga dilakukan pengamatan pola pita protein benih yang dianalisis dengan SDS-PAGE. Kandungan asam absisik (ABA) dan gula stahiosa, raftnosa, glukosa, fruktosa, arabinosa, silosa, serta sukrosa dalam benih yang ditetapkan dengan HPLC Integritas membran benih ditetapkan berdasarkan daya hantar listrik air perendaman benih yang diukur dengan konduktometer. Hasil yang diperoleh menunjukkan bahwa adanya interaksi yang nyata antara desikasi dengan seluruh tolok ukur fisiologis. Desikasi menyebabkan penurunan daya berkecambah, bobot kering dan laju pertumbuhan kecambah normal, potensi tumbuh maksimum dan kecepatan tumbuh. Sedang untuk, kandungan ABA, sukrosa, arabinosa dan rafinosa mengalami peningkatan. Di samping itu desikasi menyebabkan dibentuknya protein baru dengan BM 32,5; 47,0 dan 51,0 kDa (DR2); 47,0 dan 51,0 kD (ICS 60). Beberapa protein yang hilang oleh pengaruh desikasi yaitu dengan BM37, 0 (DR2), 19, 0 dan 37, 0 kD (ICS60). Benih ICS60 lebih tahan terhadap desikasi dibandingkan dengan benih DR2. Summary Seed of cocoa is recalcitrant and sensitive to desiccation. In open condition at room temperature, the viability of cocoa seed ultimately lost for several days. These characters are a problem for seed storage and delivery. The objectives of this study are to investigate the effect of desiccation on physiological and biochemical characters of cocoa seed. Seeds of ICS 60 (bulk cocoa) and DR2 (fine cocoa) were placed on Petri dishes and stored at 25oC, Rh 55-75% for four days (critical water content). The experiment was conducted with split plot analysis, (1) The main plot was the storage condition initial and critical seeds water content. (2) The sub plot was the variety of cocoa, with four replications of each treatment. The effect of desiccation on seeds viability was tested, based on seed water content, maximum growth potential, seed germination, germination rate, dry weight of normal seedling, and seedling growth rate. Besides, the changes of seed proteins band pattern were also analysed by SDSPAGE. Abscisic acid, stachyose, raffnose, fructose, arabinose, xyllose, and sucrose seed content were determined by HPLC. The integrity of seed membrane based on the leakage of electrolytes from seeds was measured with a CM 100 multicell conductivity meter. The results showed that there is an interaction with highly significant correlation between desiccation and all of the physiological and biochemical parameters. Desiccation caused the decrease of seed germination, dry weight and growth rate'of normal seedling, maximum growth potential, and germination rate and while the leakage of electrolytes, ABA, sucrose, arabinose and raffinose increased. Besides, desiccation was also caused the formation of new proteins with MW 32.5, 47,0 and 51,0 kDa (DR2); 47,0 and 51,0 kD ICS 60) . On the other hand, several protein were disappeared i.e. MW 37,0 (DR2), 19,0 and 37,0 kD (ICS60). Seeds of ICS 60 are more tolerant to desiccation than seeds of DR2.

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PENGARUH LAMA PEMANASAN BASAH DAN PERENDAMAN DALAM GIBERELIN TERHADAP PEMATAHAN DORMANSI BENIH KELAPA SAWIT (Elaeis guineensis jacq.)

Agrin ◽

10.20884/1.agrin.2020.24.1.508 ◽

2021 ◽

Vol 24 (1) ◽

pp. 12

Author(s):

Agustiansyah Agustiansyah ◽

Ardian Ardian ◽

Kukuh Setiawan ◽

Erni Permata Dewi

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Maximum Growth ◽

Growth Potential ◽

Randomized Design ◽

Breaking Dormancy ◽

Two Factors ◽

Heating Treatment ◽

Completely Randomized Design ◽

Four Levels

The aims of this research to determined the effect wet heating and immersion in gibberellins on breaking dormancy of oil palm seed. The treatment was arranged in factorially with two factors in the Completely Randomized Design. The first factors was wet heating which consists of five levels, (1) 20 days of heating; (2) 25 days of heating (3) 30days of heating (4) 35 days of heating (5) 40 days of heating. The second factor was the concentrations of gibberellin in four levels, (1) 0 ppm; (2) 100 ppm; (3) 200 ppm and (4) 300 ppm. Based on the results could be concluded that (1) wet heating could increased the percentage of germination, maximum growth potential, speed of germination, and time of emergence of sprouts, (2) 30 days wet heating treatment + immersion in 200 ppm giberelin was the best treatment in percentage of germination (64 % ± 1.3), maximum growth potential(70.7%±1.4), and speed of germination (29% ± 1.0 per etmal).

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i2.7222 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Wulan Dari Neng Gumiwang ◽

Tintrim Rahayu ◽

Ari Hayati

Keyword(s):

Root Length ◽

Cocos Nucifera ◽

Water Concentration ◽

Coconut Water ◽

Culture Bottle ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan

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PENGGUNAAN Trichoderma Koningii PADA PERKECAMBAHAN SIRSAK (Annona muricata linn)

AGRI-SOSIOEKONOMI ◽

10.35791/agrsosek.15.3.2019.26496 ◽

2019 ◽

Vol 15 (3) ◽

pp. 563

Author(s):

Erida Derita Dalame ◽

Bertje Richard Albert Sumayku ◽

Jeany Polii - Mandang

Keyword(s):

Seed Germination ◽

Plant Protection ◽

Germination Rate ◽

Soaking Time ◽

The North ◽

North Sulawesi ◽

Trichoderma Koningii ◽

Randomized Design ◽

Vigor Index ◽

Two Factors

This study aims to determine the dose of Trichoderma koningii which can induce soursop germination; know the best soaking time that can affect the induction of soursop germination; to know the interaction between soaking time and Trichoderma koningii dose on soursop germination. This research was conducted in April - June 2019 at the Green House of Plant Sciences, Faculty of Agriculture, Sam Ratulangi University, Manado. The material used in this study was soursop seed obtained from Lota Village, Pineleng Tomohon Subdistrict, Trichoderma koningii with 106 solids obtained from the North Sulawesi Province Plant Protection Center Collection, Aquadest, 70% Alcohol, 80% Acetone, sandalready sifter and sterilized. This research was arranged in factorial with a Completely Randomized Design (CRD) consisting of two factors, namely 5 treatments of Trichoderma koningii dose and 4 immersion treatments repeated three times (3x) so that a total of 60 treatment units. Each treatment unituses 10 seeds. The results of this study indicated that the dose of Trichoderma koninggi 200 g without soaking can increase the germination power by 86.67% and the vigor index by 32.96%; Immersion with Koninggi Trichoderma suspension has not been able to induce soursop seed germination rate; Theinteraction between Trichoderma koningii dose and soaking time did not affect the speed of soursop seed germination.*eprm*

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