scholarly journals Jerusalem Artichoke: Quality Response to Potassium Fertilization and Irrigation in Poland

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1518
Author(s):  
Bożena Bogucka ◽  
Krzysztof Jankowski
Keyword(s):  
Amino Acids ◽  
Jerusalem Artichoke ◽  
Tuber Quality ◽  
Potassium Fertilizer ◽  
High Potassium ◽  
Isoleucine Leucine ◽  
Endogenous Amino Acids ◽  
Potassium Fertilization ◽  
North Eastern ◽  
Β Carotene

The aim of this study was to determine the effects of soil potassium fertilization (150, 250 and 350 kg K2O ha−1) and irrigation on the tuber quality (content of ά-tocopherol, β-carotene, essential and endogenous amino acids) of three Jerusalem artichoke (Helianthus tuberosus L.) cultivars (Topstar, Violette de Rennes, Waldspindel). Jerusalem artichokes were grown during a field experiment in the Agricultural Experiment Station in Tomaszkowo (53°42′ N, 20°26′ E, north-eastern Poland). The content of ά-tocopherol and β-carotene was determined at 1.60–2.65 and 0.75–1.00 mg kg−1 DM, respectively, in all Jerusalem artichoke cultivars produced in north-eastern Poland. High rates of potassium fertilizer (250 and 350 kg K ha−1) increased the content of ά-tocopherol in tubers by 47% and 66% on average, respectively. The stimulatory effects of high potassium rates on the content of ά-tocopherol (2.5-fold increase) were observed only in response to irrigation. High rates of potassium fertilizer induced a particularly high increase (3.2-fold) in ά-tocopherol concentrations in Jerusalem artichokes cv. Waldspindel. Irrigation increased ά-tocopherol levels (by 40%) and decreased the concentrations of β-carotene (by 25%) and most essential and endogenous amino acids (isoleucine, leucine, lysine, phenylalanine, valine, alanine, glycine, histidine, serine, threonine). The Topstar cultivar accumulated the highest quantities of essential and endogenous amino acids. Leucine, methionine + cysteine were the limiting amino acids in Jerusalem artichoke tubers. The analyzed tubers were characterized by very high nutritional quality of dietary protein (Essential Amino-Acid Index, 66–78).

scholarly journals Moderation of Inulin and Polyphenolics Contents in Three Cultivars of Helianthus tuberosus L. by Potassium Fertilization

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 884 ◽  
Author(s):  
Anna Michalska-Ciechanowska ◽  
Aneta Wojdyło ◽  
Bożena Bogucka ◽  
Bogdan Dubis
Keyword(s):  
Jerusalem Artichoke ◽  
Polyphenolic Compounds ◽  
Potassium Fertilizer ◽  
Fertilizer Rate ◽  
Potassium Fertilization ◽  
Freeze Dried ◽  
K Fertilizer ◽  
On Line ◽  
Fertilizer Rates ◽  
Excellent Source

Jerusalem artichoke, a widely consumed edible, is an excellent source of inulin and selected phytochemicals. However, the improvement of its chemical composition by potassium fertilization has not yet been studied. Thus, the aim of the study was to evaluate the effect of different potassium (K) fertilization levels (K2O 150 kg ha−1, 250 kg ha−1, 350 kg ha−1) on the content of inulin; profile and changes in polyphenolic compounds; and the antioxidant capacity, including on-line ABTS antioxidant profiles of freeze-dried tubers originated from Violette de Rennes, Topstar, and Waldspindel cultivars. Inulin content was highest in the early maturing cv. Topstar. The application of 350 kg ha−1 of K fertilizer rates during the growth of cv. Topstar increased the inulin content of tubers by 13.2% relative to the lowest K fertilizer rate of 150 kg ha−1. In cv. Violette de Rennes, inulin accumulation increased in response to the fertilizer rate of 250 kg ha−1. A further increase in K fertilizer rates had no effect on inulin content. The inulin content of cv. Waldspindel was not modified by any of the tested K fertilizer rates. Thus, the accumulation of the inulin was cultivar-dependent. In the cultivars analyzed, 11 polyphenolic compounds were identified and polyphenolic compound content was affected by the applied rate of potassium fertilizer, which was dependent on the cultivar. Chlorogenic acid was the predominant phenolic acid in all cultivars, and it accounted for around 66.4% of the identified polyphenolic compounds in cv. Violette de Rennes and for around 77% of polyphenolic compounds in cv. Waldspindel and Topstar.

Amino acid metabolism of myeloma cells in culture

1976 ◽  
Vol 21 (3) ◽  
pp. 609-615
Author(s):  
R.S. Roberts ◽  
H.W. Hsu ◽  
K.D. Lin ◽  
T.J. Yang
Keyword(s):  
Amino Acids ◽  
Acid Metabolism ◽  
Culture Media ◽  
Growth Yield ◽  
Media Analysis ◽  
Sodium Pyruvate ◽  
Isoleucine Leucine ◽  
Myeloma Protein ◽  
Simple Modification ◽  
Myeloma Cells

The growth of myeloma cells in Leibovitz medium supplemented with 20% serum was limited by the depletion of glutamine. A simple modification of the Leibovitz medium by increasing the concentrations of glutamine, lysine, isoleucine, leucine, sodium pyruvate, galactose, and vitamins resulted in over 100% increase in cell growth yield. The total myeloma protein produced by the cells was increased by approximately 90% in modified Leibovitz media. Analysis of spent culture media for 19 amino acids showed that the concentrations of 8 amino acids were reduced; those of 5 amino acids were increased and the other 6 did not change significantly.

scholarly journals AMINO ACID COMPOSITION AND ELECTROPHORETIC PROPERTIES OF HYPERTENSIN I

1955 ◽  
Vol 102 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Leonard T. Skeggs ◽  
Walton H. Marsh ◽  
Joseph R. Kahn ◽  
Norman P. Shumway
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Isoelectric Point ◽  
Column Chromatography ◽  
Isoleucine Leucine ◽  
Ion Exchange Column

A preparation of hypertensin I was purified by countercurrent distribution and was shown to migrate as a single component in starch blocks at pH 9.3 and 4.2. It had an isoelectric point of 7.7. Quantitative analysis by ion exchange column chromatography showed eight amino acids in approximately unimolar proportion: aspartic, proline, valine, isoleucine, leucine, tyrosine, phenylalanine, and arginine. There were in addition two moles of histidine.

USE OF GLUTAMIC ACID-U-C14 TO DETERMINE NUTRITIONALLY ESSENTIAL AMINO ACIDS FOR LARVAE OF THE BLOW FLY, PHORMIA REGINA

1960 ◽  
Vol 38 (11) ◽  
pp. 1229-1234 ◽  
Author(s):  
R. Kasting ◽  
A. J. McGinnis
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Essential Amino Acids ◽  
Phormia Regina ◽  
Blow Fly ◽  
Isoleucine Leucine ◽  
Low Level ◽  
Third Instar Larvae

The production of C14O2 by third-instar larvae of the blow fly, Phormia regina Meig., after it was injected with glutamic acid-U-C14, indicates that this substrate was metabolized under these conditions. However, the nutritionally essential amino acids lysine, phenylalanine, valine, isoleucine, leucine, and threonine, isolated from the injected larvae, contained little radioactivity. A low level of radioactivity in arginine, histidine, and methionine suggests that they were slowly synthesized. The nutritionally non-essential amino acids alanine, serine, aspartic acid, and proline contained large quantities of radioactivity; tyrosine and glycine were exceptions. These results, in agreement with earlier work that used glucose-U-C14, show that radioactivity data are useful for determining certain of the nutritionally essential amino acids.

scholarly journals Effect of extruded full-fat soybeans on performance, amino acids digestibility, trypsin activity, and intestinal morphology in broilers

2013 ◽  
Vol 58 (No. 10) ◽  
pp. 470-478 ◽  
Author(s):  
M. Foltyn ◽  
V. Rada ◽  
M. Lichovníková ◽  
I. Šafařík ◽  
A. Lohniský ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Growth Performance ◽  
Intestinal Morphology ◽  
Trypsin Activity ◽  
Villus Height ◽  
Isoleucine Leucine ◽  
Final Body Weight ◽  
Dietary Treatments ◽  
Different Levels

The influence of different levels of extruded full-fat soybean (EFFSB) in the diet on growth performance, apparent ileal amino acids digestibility (AIAAD), intestinal morphology, and trypsin activity in digesta of broilers was determined. In the first experiment, two-hundred sixty ROSS 308 male chickens were used to investigate the effect of EFFSB on growth performance, intestinal morphology, and trypsin activity in the digesta and AIAAD. Five dietary treatments were used, containing 0, 40, 80, 120, and 160 g/kg of EFFSB. The experiment lasted from day 10 till day 38 of age. The inclusion of EFFSB at the level of 160 g/kg in the diet significantly (P < 0.05) decreased final body weight (2443 g in 0 group vs. 2093 in 160 group) and worsened feed efficiency. AIAAD was lower when diet contained more than 40 g/kg EFFSB. But at the level of 160 g/kg AIAAD increased in several amino acids (threonine, isoleucine, leucine, histidine). Trypsin activity increased with increasing EFFSB in the diets. There were no significant (P > 0.05) differences in AIAAD between groups 80, 120, and 160. Villus height (groups 0: 966.2; 4: 852.1; 8: 792.6; 12: 836.3; 16: 926.7 µm) and crypt depth (groups 0: 160.1; 4: 134.8; 8: 122.9; 12: 129.5; 16: 134.6 µm) of ileum decreased with inclusion of EFFSB in the diet, but villi/crypt ratio increased. In the second experiment, male chickens ROSS 308 were divided into 4 groups with 2 replicates per 100 chicks each. The groups were fed 0, 40, 80, and 120 g/kg of EFFSB. The experiment lasted from day 10 till day 38 of age. Final body weight (2594 g in 0 group vs. 2624 g in 120 group) was not significantly (P > 0.05) affected by the diet. The study showed that EFFSB at the level of 120 g/kg in grower broiler diet had no adverse effect on performance.  

scholarly journals Characterization of crtRB1 Gene Polymorphism and β-Carotene Content in Maize Landraces Originated From North Eastern Himalayan Region (NEHR) of India

2020 ◽  
Vol 4 ◽  
Author(s):  
Senthil Natesan ◽  
Thangjam Surchandra Singh ◽  
Thirusenduraselvi Duraisamy ◽  
Neelima Chandrasekharan ◽  
Sarankumar Chandran ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Himalayan Region ◽  
Carotene Content ◽  
North Eastern ◽  
Maize Landraces ◽  
Eastern Himalayan Region ◽  
Β Carotene

scholarly journals Effect of nitrogen fertilization on metabolisms of essential and non-essential amino acids in field-grown grain maize (Zea mays L.)

2010 ◽  
Vol 56 (No. 12) ◽  
pp. 574-579 ◽  
Author(s):  
T. Lošák ◽  
J. Hlušek ◽  
R. Filipčík ◽  
L. Pospíšilová ◽  
J. Maňásek ◽  
...  
Keyword(s):  
Amino Acids ◽  
Zea Mays ◽  
Field Experiments ◽  
Zea Mays L ◽  
Tricarboxylic Acid Cycle ◽  
Essential Amino Acids ◽  
Isoleucine Leucine ◽  
N Dose ◽  
Limiting Amino Acid ◽  
Nitrogen Rates

In two-year field experiments, nitrogen (N) in the form of urea (0, 120 and 240 kg N/ha) was applied to grain maize (Zea mays L.) hybrid KWS 2376. The two-year mean content of total grain N at harvest was 1.54%. The highest N dose reduced most of the 17 amino acids (AA) analysed in the grain compared with the other treatments. Possible reasons for this could be an adverse effect on the tricarboxylic acid cycle or deficiency of carbon skeletons for the assimilation of NH<sub>4</sub><sup>+</sup> into amides and amino acids. The content of the limiting amino acid lysine was not influenced by N fertilisation, with a mean two-year content of 2.02 mg/g DM. Taking into account the differences in fertilisation, the effect of the year was seen in the maximal accumulation of amino acids serine, proline, methionine, threonine, arginine and lysine. Increasing rates of nitrogen reduced the accumulation of asparagine and glycine, and, on the contrary, increased the accumulation of tyrosine. Nitrogen rates have a significant effect on the maximal accumulation of valine, isoleucine, leucine, phenylalanine, histidine, cysteine and alanine and appeared as early as after the first increased rate of nitrogen (120 kg N/ha).

scholarly journals An In Vitro Model to Investigate the Role of Helicobacter pylori in Type 2 Diabetes, Obesity, Alzheimer’s Disease and Cardiometabolic Disease

2020 ◽  
Vol 21 (21) ◽  
pp. 8369
Author(s):  
Paola Cuomo ◽  
Marina Papaianni ◽  
Clementina Sansone ◽  
Antonio Iannelli ◽  
Domenico Iannelli ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Amino Acids ◽  
Helicobacter Pylori ◽  
Bacterial Colonization ◽  
Mammalian Target Of Rapamycin ◽  
Disease Model ◽  
Cardiometabolic Disease ◽  
Isoleucine Leucine

Helicobacter pylori (Hp) is a Gram-negative bacterium colonizing the human stomach. Nuclear Magnetic Resonance (NMR) analysis of intracellular human gastric carcinoma cells (MKN-28) incubated with the Hp cell filtrate (Hpcf) displays high levels of amino acids, including the branched chain amino acids (BCAA) isoleucine, leucine, and valine. Polymerase chain reaction (PCR) Array Technology shows upregulation of mammalian Target Of Rapamycin Complex 1 (mTORC1), inflammation, and mitochondrial dysfunction. The review of literature indicates that these traits are common to type 2 diabetes, obesity, Alzheimer’s diseases, and cardiometabolic disease. Here, we demonstrate how Hp may modulate these traits. Hp induces high levels of amino acids, which, in turn, activate mTORC1, which is the complex regulating the metabolism of the host. A high level of BCAA and upregulation of mTORC1 are, thus, directly regulated by Hp. Furthermore, Hp modulates inflammation, which is functional to the persistence of chronic infection and the asymptomatic state of the host. Finally, in order to induce autophagy and sustain bacterial colonization of gastric mucosa, the Hp toxin VacA localizes within mitochondria, causing fragmentation of these organelles, depletion of ATP, and oxidative stress. In conclusion, our in vitro disease model replicates the main traits common to the above four diseases and shows how Hp may potentially manipulate them.

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