Amino acid metabolism of myeloma cells in culture
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The growth of myeloma cells in Leibovitz medium supplemented with 20% serum was limited by the depletion of glutamine. A simple modification of the Leibovitz medium by increasing the concentrations of glutamine, lysine, isoleucine, leucine, sodium pyruvate, galactose, and vitamins resulted in over 100% increase in cell growth yield. The total myeloma protein produced by the cells was increased by approximately 90% in modified Leibovitz media. Analysis of spent culture media for 19 amino acids showed that the concentrations of 8 amino acids were reduced; those of 5 amino acids were increased and the other 6 did not change significantly.
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2019 ◽
Vol 20
(7)
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pp. 644-651
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1973 ◽
Vol 28
(7-8)
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pp. 449-451
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1955 ◽
Vol 102
(4)
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pp. 435-440
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