scholarly journals Dissecting the Contribution of Environmental Influences, Plant Phenology, and Disease Resistance to Improving Genomic Predictions for Fusarium Head Blight Resistance in Wheat

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2008
Author(s):  
Jose Moreno-Amores ◽  
Sebastian Michel ◽  
Franziska Löschenberger ◽  
Hermann Buerstmayr
Keyword(s):  
Linear Regression ◽  
Fusarium Head Blight ◽  
Genomic Selection ◽  
Linear Regression Analysis ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Multiple Linear Regression Analysis ◽  
Head Blight ◽  
Severity Scores ◽  
Fhb Resistance

Environmental factors like temperature and humidity are presumed to greatly influence Fusarium head blight FHB infections in wheat. Anther retention AR, on the other hand, is a morphologically neutral trait that shares a common genetic basis with FHB resistance. In this study, our aims were to: (i) Evaluate two types of corrections of FHB severity scores, namely method-1 via linear regression on flowering time (FT), and method-2 via a best-subset multiple linear regression analysis comprising FT plus accumulated thermal time variables; and (ii) assess the performance of multi-trait genomic selection (MT.GS) models for FHB severity assisted by AR. The forward prediction scenarios where GS models were trained with data from the previous years revealed average prediction accuracies (PA) of 0.28, 0.33, and 0.36 for FHB severity scores that were uncorrected or corrected by method-1 and method-2, respectively. FHB severity scores free from the influences of both environment and phenology seemed to be the most efficient trait to be predicted across different seasons. Average PA increments up to 1.9-fold were furthermore obtained for the MT.GS models, evidencing the feasibility of using AR as an assisting trait to improve the genomic selection of FHB resistance breeding lines.

scholarly journals Alien chromatin but not Fhb7 confers Fusarium head blight resistance in wheat breeding

2021 ◽  
Author(s):  
Xianrui Guo ◽  
Qinghua Shi ◽  
Jing Yuan ◽  
Mian Wang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Glutathione S Transferase ◽  
Head Blight ◽  
Fhb Resistance ◽  
Translocation Lines ◽  
High Level ◽  
Thinopyrum Elongatum

AbstractFusarium head blight (FHB), caused by Fusarium species, seriously threaten global wheat production. Three wheat-Th.elongatum FHB resistant translocation lines have been developed and used for breeding. Transcriptomic analysis identified a derivative glutathione S-transferase transcript T26102, which was homologous to Fhb7 and induced dramatically by Fusarium graminearum. Homologs of Fhb7 were detected in several genera in Triticeae, including Thinopyrum, Elymus, Leymus, Pseudoroegeria and Roegeria. Several wheat-Thinopyrum translocation lines carrying Fhb7 remain susceptible to FHB, and transgenic plants overexpressing the T26102 on different backgrounds did not improve the FHB resistance. Taken as a whole, we show the application of the chromatin derived from diploid Thinopyrum elongatum successfully conferring wheat with high level FHB resistance independent of the Fhb7.One Sentence SummaryThinopyrum elongatum chromatin from 7EL was successfully applied to wheat FHB resistance breeding, but the resistant gene other than the reported Fhb7 remained unknown.

scholarly journals Characterizing Winter Wheat Germplasm for Fusarium Head Blight Resistance Under Accelerated Growth Conditions

2021 ◽  
Vol 12 ◽  
Author(s):  
Mustafa Zakieh ◽  
David S. Gaikpa ◽  
Fernanda Leiva Sandoval ◽  
Marwan Alamrani ◽  
Tina Henriksson ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Growth Conditions ◽  
Head Blight ◽  
Wheat Germplasm ◽  
Progress Curve ◽  
Accelerated Growth ◽  
Fhb Resistance

Fusarium head blight (FHB) is one of the economically important diseases of wheat as it causes severe yield loss and reduces grain quality. In winter wheat, due to its vernalization requirement, it takes an exceptionally long time for plants to reach the heading stage, thereby prolonging the time it takes for characterizing germplasm for FHB resistance. Therefore, in this work, we developed a protocol to evaluate winter wheat germplasm for FHB resistance under accelerated growth conditions. The protocol reduces the time required for plants to begin heading while avoiding any visible symptoms of stress on plants. The protocol was tested on 432 genotypes obtained from a breeding program and a genebank. The mean area under disease progress curve for FHB was 225.13 in the breeding set and 195.53 in the genebank set, indicating that the germplasm from the genebank set had higher resistance to FHB. In total, 10 quantitative trait loci (QTL) for FHB severity were identified by association mapping. Of these, nine QTL were identified in the combined set comprising both genebank and breeding sets, while two QTL each were identified in the breeding set and genebank set, respectively, when analyzed separately. Some QTLs overlapped between the three datasets. The results reveal that the protocol for FHB evaluation integrating accelerated growth conditions is an efficient approach for FHB resistance breeding in winter wheat and can be even applied to spring wheat after minor modifications.

scholarly journals Evaluation of Genomic Prediction for Fusarium Head Blight Resistance with a Multi-Parental Population

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 756
Author(s):  
Wentao Zhang ◽  
Kerry Boyle ◽  
Anita Brule-Babel ◽  
George Fedak ◽  
Peng Gao ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Genomic Selection ◽  
Fusarium Head Blight Resistance ◽  
Breeding Value ◽  
Minor Effect ◽  
Parental Population ◽  
Head Blight ◽  
Training Population ◽  
Environment Model ◽  
Fhb Resistance

Fusarium head blight (FHB) resistance is quantitatively inherited, controlled by multiple minor effect genes, and highly affected by the interaction of genotype and environment. This makes genomic selection (GS) that uses genome-wide molecular marker data to predict the genetic breeding value as a promising approach to select superior lines with better resistance. However, various factors can affect accuracies of GS and better understanding how these factors affect GS accuracies could ensure the success of applying GS to improve FHB resistance in wheat. In this study, we performed a comprehensive evaluation of factors that affect GS accuracies with a multi-parental population designed for FHB resistance. We found larger sample sizes could get better accuracies. Training population designed by CDmean based optimization algorithms significantly increased accuracies than random sampling approach, while mean of predictor error variance (PEVmean) had the poorest performance. Different genomic selection models performed similarly for accuracies. Including prior known large effect quantitative trait loci (QTL) as fixed effect into the GS model considerably improved the predictability. Multi-traits models had almost no effects, while the multi-environment model outperformed the single environment model for prediction across different environments. By comparing within and across family prediction, better accuracies were obtained with the training population more closely related to the testing population. However, achieving good accuracies for GS prediction across populations is still a challenging issue for GS application.

scholarly journals Characterization of the Genetic Architecture for Fusarium Head Blight Resistance in Durum Wheat: The Complex Association of Resistance, Flowering Time, and Height Genes

2020 ◽  
Vol 11 ◽  
Author(s):  
Yuefeng Ruan ◽  
Wentao Zhang ◽  
Ron E. Knox ◽  
Samia Berraies ◽  
Heather L. Campbell ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Fusarium Head Blight ◽  
Flowering Time ◽  
Plant Height ◽  
Genetic Resistance ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Cost Effective ◽  
Head Blight ◽  
Fhb Resistance

Durum wheat is an economically important crop for Canadian farmers. Fusarium head blight (FHB) is one of the most destructive diseases that threatens durum production in Canada. FHB reduces yield and end-use quality and most commonly contaminates the grain with the fungal mycotoxin deoxynivalenol, also known as DON. Serious outbreaks of FHB can occur in durum wheat in Canada, and combining genetic resistance with fungicide application is a cost effective approach to control this disease. However, there is limited variation for genetic resistance to FHB in elite Canadian durum cultivars. To explore and identify useful genetic FHB resistance variation for the improvement of Canadian durum wheat, we assembled an association mapping (AM) panel of diverse durum germplasms and performed genome wide association analysis (GWAS). Thirty-one quantitative trait loci (QTL) across all 14 chromosomes were significantly associated with FHB resistance. On 3BS, a stable QTL with a larger effect for resistance was located close to the centromere of 3BS. Three haplotypes of Fhb1 QTL were identified, with an emmer wheat haplotype contributing to disease susceptibility. The large number of QTL identified here can provide a rich resource to improve FHB resistance in commercially grown durum wheat. Among the 31 QTL most were associated with plant height and/or flower time. QTL 1A.1, 1A.2, 3B.2, 5A.1, 6A.1, 7A.3 were associated with FHB resistance and not associated or only weakly associated with flowering time nor plant height. These QTL have features that would make them good targets for FHB resistance breeding.

Masked mycotoxins: does breeding for enhanced Fusarium head blight resistance result in more deoxynivalenol-3-glucoside in new wheat varieties?

2016 ◽  
Vol 9 (5) ◽  
pp. 741-754 ◽  
Author(s):  
M. Lemmens ◽  
B. Steiner ◽  
M. Sulyok ◽  
P. Nicholson ◽  
A. Mesterhazy ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Animal Health ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Head Blight ◽  
Concentration Data ◽  
Relative Level ◽  
Wheat Varieties ◽  
Fhb Resistance ◽  
Highly Correlated

From economic and environmental points of view, enhancing resistance to Fusarium head blight (FHB) in wheat is regarded as the best option to reduce fungal colonisation and the concomitant mycotoxin contamination. This review focuses on the effect of FHB resistance on deoxynivalenol (DON) and the masked metabolite deoxynivalenol-3-glucoside (DON-3-glucoside) in wheat. Based on published information complemented with our own results we draw the following conclusions: (1) All investigated wheat cultivars can convert DON to DON-3-glucoside. Hence, detoxification of DON to DON-3-glucoside is not a new trait introduced by recent resistance breeding against FHB. (2) The amount of DON-3-glucoside relative to DON contamination can be substantial (up to 35%) and is among other things dependent on genetic and environmental factors. (3) Correlation analyses showed a highly significant relationship between the amount of FHB symptoms and DON contamination: breeding for FHB resistance reduces DON contamination. (4) DON contamination data are highly correlated with DON-3-glucoside concentration data: in other words, reduction of DON content through resistance breeding results in a concomitant reduction in DON-3-glucoside content. (5) The DON-3-glucoside/DON ratio increases with decreasing DON contamination: the most resistant lines with the lowest DON contamination show the highest relative level of DON-3-glucoside to DON. In summary, introgressing FHB resistance reduces both DON and DON-3-glucoside levels in the grain, but the reduction is lower for the masked toxin. DON-3-glucoside can represent a possible hazard to human and animal health, especially in wheat samples contaminated with DON close to permitted limits.

scholarly journals A Quantitative Proteomics View on the Function of Qfhb1, a Major QTL for Fusarium Head Blight Resistance in Wheat

Pathogens ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 58 ◽  
Author(s):  
Moustafa Eldakak ◽  
Aayudh Das ◽  
Yongbin Zhuang ◽  
Jai Rohila ◽  
Karl Glover ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Starch Synthesis ◽  
Resistance Mechanism ◽  
Fusarium Head Blight Resistance ◽  
Defense Responses ◽  
Wheat Breeding ◽  
Host Cells ◽  
Head Blight ◽  
Fhb Resistance ◽  
Trait Locus

Fusarium head blight (FHB) is a highly detrimental disease of wheat. A quantitative trait locus for FHB resistance, Qfhb1, is the most utilized source of resistance in wheat-breeding programs, but very little is known about its resistance mechanism. In this study, we elucidated a prospective FHB resistance mechanism by investigating the proteomic signatures of Qfhb1 in a pair of contrasting wheat near-isogenic lines (NIL) after 24 h of inoculation of wheat florets by Fusarium graminearum. Statistical comparisons of the abundances of protein spots on the 2D-DIGE gels of contrasting NILs (fhb1+ NIL = Qfhb1 present; fhb1- NIL = Qfhb1 absent) enabled us to select 80 high-ranking differentially accumulated protein (DAP) spots. An additional evaluation confirmed that the DAP spots were specific to the spikelet from fhb1- NIL (50 spots), and fhb1+ NIL (seven spots). The proteomic data also suggest that the absence of Qfhb1 makes the fhb1- NIL vulnerable to Fusarium attack by constitutively impairing several mechanisms including sucrose homeostasis by enhancing starch synthesis from sucrose. In the absence of Qfhb1, Fusarium inoculations severely damaged photosynthetic machinery; altered the metabolism of carbohydrates, nitrogen and phenylpropanoids; disrupted the balance of proton gradients across relevant membranes; disturbed the homeostasis of many important signaling molecules induced the mobility of cellular repair; and reduced translational activities. These changes in the fhb1- NIL led to strong defense responses centered on the hypersensitive response (HSR), resulting in infected cells suicide and the consequent initiation of FHB development. Therefore, the results of this study suggest that Qfhb1 largely functions to either alleviate HSR or to manipulate the host cells to not respond to Fusarium infection.

scholarly journals Epigenetic regulation of gene expression improves Fusarium head blight resistance in durum wheat

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jitendra Kumar ◽  
Krishan M. Rai ◽  
Seyedmostafa Pirseyedi ◽  
Elias M. Elias ◽  
Steven Xu ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Regulation Of Gene Expression ◽  
Fusarium Head Blight Resistance ◽  
Mapk Signaling ◽  
Head Blight ◽  
Breeding Lines ◽  
Significant Difference ◽  
Plant Pathogen Interaction ◽  
Fhb Resistance ◽  
Parental Lines

Abstract Eight advanced durum-breeding lines were treated with 5-methyl-azacytidine to test the feasibility of generating sources of Fusarium head blight (FHB) resistance. Of the 800 treated seeds, 415 germinated and were advanced up to four (M4) generations by selfing. Thirty-two of the resulting 415 M4 lines were selected following preliminary screening and were further tested for FHB resistance for three years at two field locations, and in the greenhouse. Five of the 32 M4 lines showed less than 30% disease severity, as compared to the parental lines and susceptible checks. Fusarium-damaged kernels and deoxynivalenol analyses supported the findings of the field and greenhouse disease assessments. Two of the most resistant M4 lines were crossed to a susceptible parent, advanced to third generation (BC1:F3) and were tested for stability and inheritance of the resistance. About, one third of the BC1:F3 lines showed FHB resistance similar to their M4 parents. The overall methylation levels (%) were compared using FASTmC method, which did not show a significant difference between M4 and parental lines. However, transcriptome analysis of one M4 line revealed significant number of differentially expressed genes related to biosynthesis of secondary metabolites, MAPK signaling, photosynthesis, starch and sucrose metabolism, plant hormone signal transduction and plant-pathogen interaction pathways, which may have helped in improved FHB resistance.

scholarly journals Proteomics Reveals the Changes that Contribute to Fusarium Head Blight Resistance in Wheat

2020 ◽  
pp. PHYTO-05-20-017
Author(s):  
Mingming Yang ◽  
Xianguo Wang ◽  
Jian Dong ◽  
Wanchun Zhao ◽  
Tariq Alam ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Metabolic Pathways ◽  
Molecular Mechanisms ◽  
Critical Role ◽  
Functional Characterization ◽  
Fusarium Head Blight Resistance ◽  
Defense Responses ◽  
Head Blight ◽  
Resistance Response ◽  
Fhb Resistance

Fusarium head blight (FHB) is a devastating disease of wheat, causing yield losses and quality reduction as a result of mycotoxin production. In this study, iTRAQ (isobaric tags for relative and absolute quantification)- labeling-based mass spectrometry was employed to characterize the proteome in wheat cultivars Xinong 538 and Zhoumai 18 with contrasting levels of FHB resistance as a means to elucidate the molecular mechanisms contributing to FHB resistance. A total of 13,669 proteins were identified in the two cultivars 48 h after Fusarium graminearum inoculation. Among these, 2,505 unique proteins exclusively accumulated in Xinong 538 (resistant) and 887 proteins in Zhoumai 18 (susceptible). Gene Ontology enrichment analysis showed that most differentially accumulated proteins (DAPs) from both cultivars were assigned to the following categories: metabolic process, single-organism process, cellular process, and response to stimulus. Kyoto Encyclopedia of Genes and Genomes analysis showed that a greater number of proteins belonging to different metabolic pathways were identified in Xinong 538 compared with Zhoumai 18. Specifically, DAPs from the FHB-resistant cultivar Xinong 538 populated categories of metabolic pathways related to plant–pathogen interaction. These DAPs might play a critical role in defense responses exhibited by Xinong 538. DAPs from both genotypes were assigned to all wheat chromosomes except chromosome 6B, with approximately 30% mapping to wheat chromosomes 2B, 3B, 5B, and 5D. Twenty single nucleotide polymorphism markers, flanking DAPs on chromosomes 1B, 3B, 5B, and 6A, overlapped with the location of earlier mapped FHB-resistance quantitative trait loci. The data provide evidence for the involvement of several DAPs in the early stages of the FHB-resistance response in wheat; however, further functional characterization of candidate proteins is warranted.

scholarly journals Evaluation of Canadian barley breeding lines for Fusarium head blight resistance

2015 ◽  
Vol 95 (5) ◽  
pp. 923-929 ◽  
Author(s):  
Xinyao He ◽  
Mohamed Osman ◽  
James Helm ◽  
Flavio Capettini ◽  
Pawan K. Singh
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Correlation Coefficients ◽  
Blight Resistance ◽  
Head Blight ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Barley Breeding ◽  
Days To Heading ◽  
Fhb Resistance

He, X., Osman, M., Helm, J., Capettini, F. and Singh, P. K. 2015. Evaluation of Canadian barley breeding lines for Fusarium head blight resistance. Can. J. Plant Sci. 95: 923–929. Fusarium head blight (FHB) is a major challenge to the successful production of barley in Canada, as well as for end-users such as the malting and brewing industries. Due to the quantitative inheritance of FHB resistance, continuous effort is required to identify breeding lines with improved FHB resistance and incorporate them into crossing schemes to enhance FHB resistance. In the present study, 402 advanced breeding lines from Alberta, Canada, were evaluated in the FHB screening nursery at CIMMYT, Mexico. In 2011 and 2012, FHB incidence was measured on a scale of 1 to 4 to eliminate the most susceptible lines. In 2013 and 2014, 181 lines with the lowest disease scores in the previous 2 yr were tested in replicated experiments for field FHB index, Fusarium-damaged kernels, and deoxynivalenol content. Agronomic and morphological traits, specifically days to heading, plant height, and row and hull types were also evaluated in relations to FHB parameters. Correlation coefficients among the three FHB parameters in both 2013 and 2014 were all significant at P<0.0001, ranging from 0.36 to 0.63. Additional correlation analysis showed that late-maturing, tall, and two-row lines tended to have lower disease, whereas hull type did not show a significant correlation with FHB. Several lines with high and stable FHB resistance similar to that of the resistant checks were identified. These could be used in breeding programs as resistance sources or be registered as new cultivars if their overall attributes meet commercial standards.

scholarly journals The Ability to Detoxify the Mycotoxin Deoxynivalenol Colocalizes With a Major Quantitative Trait Locus for Fusarium Head Blight Resistance in Wheat

2005 ◽  
Vol 18 (12) ◽  
pp. 1318-1324 ◽  
Author(s):  
Marc Lemmens ◽  
Uwe Scholz ◽  
Franz Berthiller ◽  
Chiara Dall'Asta ◽  
Andrea Koutnik ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Fusarium Head Blight ◽  
Quantitative Trait ◽  
Fusarium Head Blight Resistance ◽  
Close Relation ◽  
Major Effect ◽  
Head Blight ◽  
Fhb Resistance ◽  
Trait Locus ◽  
Wheat Lines

We investigated the hypothesis that resistance to deoxynivalenol (DON) is a major resistance factor in the Fusarium head blight (FHB) resistance complex of wheat. Ninety-six double haploid lines from a cross between ‘CM-82036’ and ‘Remus’ were examined. The lines were tested for DON resistance after application of the toxin in the ear, and for resistances to initial infection and spread of FHB after artificial inoculation with Fusarium spp. Toxin application to flowering ears induced typical FHB symptoms. Quantitative trait locus (QTL) analyses detected one locus with a major effect on DON resistance (logarithm of odds = 53.1, R2 = 92.6). The DON resistance phenotype was closely associated with an important FHB resistance QTL, Qfhs.ndsu-3BS, which previously was identified as governing resistance to spread of symptoms in the ear. Resistance to the toxin was correlated with resistance to spread of FHB (r = 0.74, P < 0.001). In resistant wheat lines, the applied toxin was converted to DON-3-O-glucoside as the detoxification product. There was a close relation between the DON-3-glucoside/DON ratio and DON resistance in the toxintreated ears (R2 = 0.84). We conclude that resistance to DON is important in the FHB resistance complex and hypothesize that Qfhs.ndsu-3BS either encodes a DON-glucosyltransferase or regulates the expression of such an enzyme.

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