scholarly journals Breeding for Prolificacy, Total Carotenoids and Resistance to Downy Mildew in Small-Ear Waxy Corn by Modified Mass Selection

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1793
Author(s):  
Supaporn Sukto ◽  
Khomsorn Lomthaisong ◽  
Jirawat Sanitchon ◽  
Sompong Chankaew ◽  
Shanerin Falab ◽  
...  

The study aimed to improve the small-ear waxy corn populations for prolificacy, high total carotenoid content and resistance to downy mildew. Three cycles of modified mass selection were carried out for population improvement. Forty-four genotypes derived from eight C3 populations and six check varieties were evaluated for agronomic traits and yield at Khon Kaen and screened for downy mildew resistance in the rainy season 2020 at two experimental sites in Ban Phang district of Khon Kaen province. Fifty genotypes were clustered into six major groups based on color parameter (h°) and total ear number. Two selected groups (C and E) with two populations of small-ear waxy corn including Nei9008/BK-24-9-B and TY/TF-33-1-B were selected as they were resistant to downy mildew, prolific ears, and intense orange kernel color. Modified mass selection was effective for improvement of multiple traits in waxy corn.

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Supaporn Sukto ◽  
Khomsorn Lomthaisong ◽  
Jirawat Sanitchon ◽  
Sompong Chankaew ◽  
Marvin P. Scott ◽  
...  

Waxy corn is a popular, alternative staple food in most Asian countries including Thailand. The availability of small-ear waxy corn genotypes with prolific ears and a high level of carotenoids is expected to benefit growers and consumers. Integrated evaluation among source germplasm is essential before performing further breeding efforts for enhancing prolific ears and high-carotenoid content. Thus, the present study explored the variability of ear prolificacy, total carotenoids, lutein, zeaxanthin, and beta-carotene among yellow small-ear waxy corn accessions. About 44 corn accessions and 4 check varieties were evaluated for agronomic traits and yield components under multienvironment trials in a randomized complete block design (RCBD). The immature seed sample of these genotypes was analyzed to quantify the content of total carotenoids and some carotenoid fractions. All traits showed that low GXE interaction and significant genotypic diversity existed among all tested accessions with the predominant contribution of genotype to total phenotypic variation and beta-carotene. Accessions were clustered into four major groups based on the similarity of multiple carotenoids profiles. Three selected accessions (UT121001, KKU-WX112087, and KKU-WX212001) had higher values of total carotenoids, lutein, zeaxanthin, and beta-carotene than those of all check varieties. High and positive correlations among second-emerged ears, marketable second-emerged ears, and total ear number indicate that a higher chance of secondary ears becomes marketable ears with an increase of total ears per plant per hectare. Lutein and zeaxanthin had positive, strong correlations with total carotenoids. The implications and breeding strategies are discussed prior to promoting yellow small-ear waxy corn as a biofortified crop.


Author(s):  
Elena Andreea POP ◽  
Andrea BUNEA ◽  
Florina COPACIU ◽  
Carmen SOCACIU ◽  
Adela PINTEA

Apricots are well known for the high content of bioactive compounds such as carotenoids, polyphenols, vitamins and minerals. Several studies have pointed out the chemical composition or the biological effects of apricots, but limited information are available regarding the stability of active compounds during storage or processing. The aim of this study was to determine the stability of major carotenoids in commercial dried apricots during storage.Carotenoids were extracted monthly from dried apricots kept in a dark environment, at room temperature, for twelve months. Total carotenoids were determined using the spectrophotometric method while the most relevant carotenoids were analyzed by high-performance liquid chromatography-photodiode array detection (HPLC-PDA) on a C30 column and using a gradient elution system.Initial carotenoid content of dried fruits was 6.72 mg/100g, while after six months of storage it decreased to 2.46 mg/100g. After twelve months of storage the total carotenoid content was 0.82 mg/100g, representing 20.35 % of the initial concentration. The major carotenoids identified in apricots were: all trans β-carotene, its geometrical isomers (9-cis-β-carotene; 13-cis-β-carotene; 9,13-di-cis β-carotene); β-carotene-5,8-epoxide; β-cryptoxanthin and β-cryptoxanthin palmitate. Significant decreases were observed for all pigments but all trans β-carotene appears to be the most sensitive pigment, with 15.7 % residual concentration. Although the concentrations of β-cryptoxanthin palmitate is small, it has shown significant increased stability compared to carotenes.


2012 ◽  
Vol 24 (1) ◽  
pp. 33-39
Author(s):  
Wanda Wadas ◽  
Halina Mioduszewska ◽  
Krzysztof Kalinowski

Effects of selected agronomical factors on the content of health-promoting compounds in spaghetti squash (Cucurbita pepoL. subsp.pepo)The study examined the effects of the sowing date (5, 15 and 25 May) and plant spacing (1 × 0.6 m, 1 × 0.8 m and 1 × 1 m) on the content of carotenoids and L-ascorbic acid in the fruits of two cultivars of spaghetti squash (‘Makaronowa Warszawska' and ‘Pyza’). The field experiment was carried out in central-eastern Poland. Fully mature spaghetti squash fruits were harvested from the beginning of September to the beginning of October. The content of total carotenoids and β-carotene was the greatest in the fruits of plants sown on 15 May. The sowing date did not affect the content of L-ascorbic acid. Increasing the plant spacing from 1 × 0.6 m to 1 × 1 m resulted in an increase of total carotenoid content, particularly for the spaghetti squash sown on 25 May. Plant spacing did not have a significant effect on the content of β-carotene and L-ascorbic acid. Fruits of the ‘Makaronowa Warszawska’ with light orange flesh contained more total carotenoids and β-carotene than fruits of the ‘Pyza’ with cream-coloured flesh. The content of L-ascorbic acid in the fruits of both cultivars did not differ significantly.


Author(s):  
Alena Andrejiová ◽  
Alžbeta Hegedűsová ◽  
Miroslav Šlosár ◽  
Silvia Barátová

The winter squash is an important source of antioxidants, especially carotenoids. The aim of submitted research work was to determine the effect of genotype, storage and different methods of technological processing (baking, boiling and sterilization) on the content of ascorbic acid and total carotenoids in fruits of winter squash (Cucurbita moschata Duch. ex Poir.). The small-plot field experiment was established at Slovak University of Agriculture in Nitra in 2013. Five cultivars of winter squash (‘Liscia’, ‘Orange’, ‘Hannah’, ‘UG 205 F1’ and ‘Waltham’) were examined in experiment. The total carotenoids content in the pulp of fresh fruits was ranged from 9.33 to 15.10 mg.100 g−1. Its highest value was determined in case of ‘Orange’ variety. The storage and the thermal treatment of fruit pulp in case of baking had positive impact from the total carotenoid content point of view. The baking resulted in the increase of its value in winter squash. On the contrary, sterilization tended to the decrease of total carotenoid content in edible part of squash. The total carotenoids content in the baking pulp was ranged from 14.27 to 31.87 mg.100 g−1. The vitamin C content before storage and technological processing ranged in interval from 13.88 to 18.69 mg.100 g−1. Particular thermal methods of processing and storage resulted in decrease of vitamin C content in the pulp of all winter squash varieties.


2015 ◽  
Vol 43 (3) ◽  
pp. 255-260
Author(s):  
Gyanendra Kumar Rai ◽  
Rajesh Kumar ◽  
Ranjeet Ranjan Kumar ◽  
Sheetal Dogra

Significant differences (p ? 0.05) were observed among the cherry tomato lines for the principal antioxidants, viz. total carotenoids, lycopene and vitamin-C. Vitamin-C content ranged from 17.62 - 46.16 mg/100 g, the total carotenoid content ranged from 3.86 - 6.66 mg/100 g and lycopene content ranged from 2.83 - 5.26 mg/100 g on fresh weight basis. The total phenolics, another important class of antioxidants, also differed significantly amongst the cherry tomato line (12.41-31.17 mg gallic acid equivalent/100 g). Significant variation (p ? 0.05) was also observed for pH and titrable acidity. The pH varied from 4.15 - 4.52 and anhydrous citric acid ranged from 0.050 up to 0.323%. The total soluble solids were 3.41 - 5.16%. The maximum vitamin-C content was recorded in VRCT-6 (46.16 mg/100 g) closely followed by VRCT-7 (45.51 mg/100 g) and VRCT-15 (44.71 mg/100 g), whereas maximum total carotenoid content were recorded in VRCT-16 (6.66 mg/100 g) followed by VRCT-7 (6.48 mg/100 g) and VRCT-1(6.36 mg/100 g), respectively. On the other hand, maximum lycopene content was estimated in VRCT-16 (5.26 mg/100 g) followed by VRCT-3 (5.23 mg/100 g) and VRCT-14 (4.73 mg/100 g). Maximum acidity and total water soluble solids were recorded in VRCT-9 (0.323%) and VRCT-7 (5.16%), respectively. DOI: http://dx.doi.org/10.3329/bjb.v43i3.21591 Bangladesh J. Bot. 43(3): 255-260, 2014 (December)


2019 ◽  
Vol 13 ((04) 2019) ◽  
pp. 566-573 ◽  
Author(s):  
Watanyoo Khamkoh ◽  
Danupol Ketthaisong ◽  
Khomsorn Lomthaisong ◽  
Kamol Lertrat ◽  
Bhalang Suriharn

The information on the responses to simple recurrent selection for yield and total carotenoid content in orange waxy corn is lacking. The objectives were to evaluate the responses to simple recurrent selection for two cycles for yield and carotenoids of orange waxy corn population and to investigate the correlations between color parameter with carotenoids in waxy corn kernels. Parental sources of germplasm with differences in kernel colors and levels of disease resistance were used for generation of a base population of orange waxy corn. A simple recurrent selection for superior phenotypes was practiced on this population for two selection cycles. Cycle 0 (C0), C1 and C2 were evaluated for two locations in 2016. The cycles showed significant differences for most traits except for plant height. The locations contributed to large portions of total variation in total yield, whereas selection cycle contributed to large portions of total variations in lutein, zeaxanthin, β-carotene and total carotenoid content and color parameter (ho), indicating that selection for carotenoids would be possible. Simple recurrent selection for two cycles increased total yield and husked ear weight of 23.2% and 15.0%, respectively. Simple recurrent selection for two cycles also increased lutein, zeaxanthin, β-carotene and total carotenoid content with increasing percentages ranging from 18.5 to 196.6%. The results indicated that population improvement by simple recurrent selection could be useful in increasing these traits. Color parameter at immature stage and dry stage was negatively and significantly correlated with lutein, zeaxanthin, β-carotene and total carotenoid content, indicating that color parameter can be used as an indirect selection criterion for these traits.


2021 ◽  
Vol 24 ◽  
Author(s):  
Bruna Lago Tagliapietra ◽  
Alencar Zanon Junior ◽  
Luana Fernandes Tironi ◽  
Nereu Augusto Streck ◽  
Neila Silvia Pereira dos Santos Richards

Abstract It is estimated that the global number of people affected by micronutrient deficiency, known worldwide as hidden hunger, is over two billion and also considered a public health problem. Therefore, this study aimed to evaluate biofortified cassava cultivars in relation to their physical-chemical composition, total carotenoids and to verify their sensory acceptance by schoolchildren. The study was conducted in Santa Maria, in the state of Rio Grande do Sul (RS), Brazil, with four cassava cultivars, two biofortified with yellow pulp (BRS 399 and BRS 396); two non-biofortified varieties: one with yellow pulp (“Gema de ovo”) and another with white pulp (“Vassourinha”). After cooking the roots, the nutritional composition (moisture, protein, lipid, ash, and total starch), the total carotenoid content, and the sensory acceptance of the roots by the children at school, in the countryside of Julio de Castilhos and Dilermando de Aguiar, was determined. The contents of moisture, protein, lipids, ash, and starch showed significant variations between the cultivars analyzed. The highest levels of total carotenoids were found in biofortified cultivars (12.85 µg g-1). In the non-biofortified cultivars, “Gema de ovo” and “Vassourinha”, the levels were 12.01 µg g-1 and 3.30 µg g-1, respectively. Sensory acceptance was 78.7%, demonstrating the potential for the insertion of biofortified roots in school meals.


2021 ◽  
Author(s):  
Seyed Nesa Mousavi ◽  
Hamid Mohammadiazarm ◽  
Fatemeh Azadbakht

Abstract In this experiment, the influences of dietary garlic, onion, and sweet red pepper meals as herbal supplements were studied on some immunological parameters, total carotenoid content, and growth performance of male guppy fish (Poecilia reticulata) in the juvenile stage. The experimental diets were fed to fish as a control (0), and three other diets contained 20 g kg− 1 garlic, or 20 g kg− 1 red onion, and or 20 g kg− 1 sweet red pepper powders. The male guppies with the early weight of 0.642g ± 0.07 were stocked in twelve circular tanks (40 L capacity) at density of 30 fish per tank. Fish were fed to satiation the diets thrice daily. The results showed that dietary herbal additives induced a significant increase (P < 0.05) in some mucosal immunological parameters contained alternative complement (ACH50), total immunoglobulin (Ig), lysozyme, and total protein compared to control, which were the highest in the group with 20 g kg− 1 garlic powder. Also, various dietary herbal additives resulted in higher (P < 0.05) total carotenoids levels of lateral skin body and caudal fin of fish than control, which were the highest in the group with 20 g kg− 1 sweet red pepper. The growth performance and feeding indices enhanced significantly (P < 0.05) in experimental groups compared to the control. As a result, used herbal supplements had beneficial effects on immunological parameters and total carotenoid content of fish. So, the highest immunity and coloration was obtained separately at 20 g kg− 1 respectively with garlic and sweet red pepper meals.


Beverages ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 43
Author(s):  
Stavros Lalas ◽  
Aggeliki Alibade ◽  
Eleni Bozinou ◽  
Dimitris P. Makris

In this study, peach pomace (PP) moisture reduction using conventional oven-drying was investigated by implementing the Box-Behnken experimental design, considering two major process variables, time—t and temperature—T. The purpose was the optimisation of the process to obtain PP extracts as rich as possible in total carotenoids (TCn). It was shown that effective moisture removal up to a final level of approximately 24%, could be achieved after 8.27 h (496 min) at 70 °C. Under these optimised drying conditions, the maximum carotenoid yield was 84.57 ± 8.56 μg CtE g−1 dm. This yield was by almost 63% lower than that achieved using fresh (non-dried) samples. Temperatures higher than 70 °C were demonstrated to be even more detrimental in this regard, yet from the model built, it was made clear that prolonged drying time may bring about a more pronounced negative effect on the total carotenoid yield. The drop in total carotenoid content of PP as a result of drying was accompanied by a significant decline in the antiradical activity of PP extracts.


Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 196
Author(s):  
Ancuta Nartea ◽  
Benedetta Fanesi ◽  
Pasquale Massimiliano Falcone ◽  
Deborah Pacetti ◽  
Natale Giuseppe Frega ◽  
...  

The effect of steam and sous-vide oven procedures on liposoluble antioxidants of colored cauliflower (orange and purple) was assessed for the first time and compared with domestic practice (boiling). In raw samples, the total carotenoid content was 10-fold higher in Cheddar than in Depurple (20.9 ± 2.1 vs. 2.3 ± 0.5 mg/kg dry weight), whereas the level of tocopherols was similar (28.5 ± 4.4 vs. 33 ± 5.2 mg/kg dry weight). The Cheddar liposoluble antioxidant matter contained violaxanthin, neoxanthin, α-carotene and δ-tocopherol, not detected in Depurple. All tests increased the bioactive compounds extractability with steam oven and sous-vide displaying similar effects, lower than boiling. In boiled Cheddar cauliflower, the total carotenoids and tocopherols contents increased with cooking time until they were 13-fold and 6-fold more than in raw cauliflower, respectively. Conversely, in the Depurple variety, contents increased by half with respect to the orange variety. However, from a nutritional point of view, no differences were revealed among the three different cooking treatments in terms of vitamin A and E levels expressed in μg/100 g of fresh vegetable because of the higher water content of boiled samples that must be considered when evaluating the effect of thermal treatment on cauliflower nutritional traits.


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