scholarly journals Assessment of the Genetic Diversity of Chrysanthemum Cultivars Using SSR Markers

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2318
Author(s):  
Agata Olejnik ◽  
Katarzyna Parkitna ◽  
Bartosz Kozak ◽  
Szymon Florczak ◽  
Jakub Matkowski ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Morphological Characteristics ◽  
Economic Importance ◽  
Flowering Plants ◽  
Mutual Distance ◽  
High Interest ◽  
The World ◽  
Efficient Breeding ◽  
Phenotypic Methods

Chrysanthemums are undoubtedly one of the most popular flowering plants in the world. Their exceptional importance in Asian culture resulted in the global popularization of this species, which resulted in the high interest of breeders. Chrysanthemums can be divided into three groups: small-flowered, mid-flowered, and large-flowered. The exceptional economic importance and a large number of varieties make them problematic to identify, resulting in a less efficient breeding process. In the case of chrysanthemums, genotypes are almost impossible to distinguish by using phenotypic methods due to the high variation in morphological characteristics, even when they belong to the same group. The aim of the study was to evaluate the genetic diversity of 97 chrysanthemum cultivars using 14 selected SSR markers. Large-flowered varieties (Angali and Rosee D’une) were characterized by the smallest mutual distance, and the greatest distance was between large-flowered (Impact Rood) and small-flowered (Conaco Yellow) varieties. All methods of visualizing the results reveal a clear distinctiveness of small-flowered cultivars, except for the cultivars from the Moira series.

Genetic diversity among potato species as revealed by phenotypic resistances and SSR markers

2013 ◽  
Vol 11 (2) ◽  
pp. 131-139 ◽  
Author(s):  
D. Carputo ◽  
D. Alioto ◽  
R. Aversano ◽  
R. Garramone ◽  
V. Miraglia ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Sources Of Resistance ◽  
Wild Potato Species ◽  
Resistance Response ◽  
Potato Species ◽  
Average Value ◽  
Ssr Analysis ◽  
Efficient Breeding ◽  
Resistant Genotypes

The evolutionary diversity of wild potato species makes them excellent materials for improving the narrow genetic basis of the cultivated potato Solanum tuberosum. Understanding their genetic diversity is important not only to choose the best parents for breeding, but also to design proper crossing schemes and selection strategies. The objectives of this study were to determine the resistance response to Ralstonia solanacearum, Potato virus Y and low temperatures of 21 clones of 12 potato species, and to determine their genetic diversity through simple sequence repeat (SSR) markers. Sources of resistance have been found for all the investigated traits, with high resistance variability not only between but also within species. Combined resistances were also identified, with positive implications for efficient breeding. SSR analysis allowed the detection of 12 loci and 46 alleles across all genotypes, with an average value of 3.8 alleles per locus. Both unique and rare alleles useful for marker-assisted selection were found. SSR-based cluster analysis revealed that resistant genotypes were distributed among all clusters, suggesting that genetically different resistant genotypes were identified. The information obtained in this study is discussed from a breeding perspective.

scholarly journals Characterization of quince (Cydonia oblonga Mill.) cultivars using SSR markers developed for apple

2009 ◽  
Vol 15 (4) ◽  
Author(s):  
J. Halász ◽  
V. Hoffman ◽  
Z. Szabó ◽  
J. Nyéki ◽  
T. Szabó ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Genetic Bottleneck ◽  
The Self ◽  
Cydonia Oblonga ◽  
Allele Number ◽  
The World ◽  
Ssr Loci ◽  
A Minor

Quince (Cydonia oblongaMill.) is a minor fruit crop, which is primarily used for marmalade, jam and sauce.Very few quince cultivars are known all over the world and in many cases similar names are used for presumably different cultivars. The aim of the present study was to evaluate and characterize the genetic diversity of 36 quince cultivars and selections with SSR markers. Seven out of 8 SSR markers designed from apple sequences could successfully yield amplification also in quince cultivars. Number of alleles per locus ranged from 2 to 3 alleles. These allele numbers are quite low when compared to apple. It is supposed to be the consequence of a genetic bottleneck. In spite of the low allele number per locus, the 36 quince cultivars formed 30 different genotypes. The ratio of homozygosity was low, which might be coupled with the self-(in)compatibility phenotype of quinces. SSR markers proved unable to differentiate putatively closely related cultivars (e.g. ‘Bereczki’ and ‘Bereczki bôtermő’). In general, the level of polymorphism among the tested quince genotypes was much restricted due to the low allele number detected. However, it must be considered that the number of analysed SSR loci is not enough high to estimate the overall heterozygosity of the quince genome. Further experiments are needed and the SSR markers proved to be a reliable and useful tool for such analyses.

scholarly journals Evaluation of Genetic Diversity in Iranian Rice (Oryza Sativa L.) Cultivars for Resistance Blast Disease (Magnaporthe Oryzae) Using Microsatellite (SSR) Markers.

2019 ◽  
Author(s):  
Farzaneh Farahzadi ◽  
Asa Ebrahimi ◽  
Vahid Zarrinnia ◽  
Reza Azizinezhad
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Magnaporthe Oryzae ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Molecular Data ◽  
Blast Disease ◽  
Analysis Group ◽  
The People ◽  
The World

AbstractRice plays an important role in feeding the world’s population, specially the people who live in developing countries. Rice occupies one third of the world’s crops and provides 35 to 60 percent of calories consumed by 2.7 billion people in the world. More than 90 percent of world’s rice is produced and consumed in Asia. Blast disease caused by Magnaporthe Oryzae is one of the most important diseases of rice in the world. This study was evaluated the Power of ten blast specific SSR Markers in determination genetic diversity between thirty cultivars of Indica rice. The phenotypic evaluation was also performed using Magnaporthe Oryzae in stage of three or four leaves. The total of 23 effective alleles with an average of 2.21 alleles per locus affecting microsatellite markers were amplified in genotypes. The total of 28 alleles was also observed per locus with 2.8 average. Also, the average of PIC was estimated 42.0 for markers. The RM204 marker has the maximum of PIC (0.60) and RM413 marker has the minimum of PIC (0.081). The calculated similarities result between molecular data by Jacard similarity coefficient and UPGMA algorithm have divided thirty studied genotypes into three categories. This analysis between coordinates decides the cluster analysis group. In phenotypic testing, the calculated similarities between phenotypic has also occurred using coefficient similarity Dist and UPGMA algorithm. According to phenotypic evaluation the population was also divided into three groups. According to T-Student test for comparing the phenotypic and genotypic data, SSR markers "RM277" and “RM8225” were linked to semi-susceptible, and resistant phenotype.

scholarly journals Genetic Diversity Within and Among Populations of Roseroot (Rhodiola rosea L.) Based on Molecular Markers

2012 ◽  
Vol 40 (2) ◽  
pp. 266 ◽  
Author(s):  
Zsuzsanna GYORGY ◽  
Maria SZABO ◽  
Dmitry BACHAROV ◽  
Andrzej PEDRYC
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gene Diversity ◽  
Geographic Origin ◽  
Morphological Characteristics ◽  
Population Level ◽  
Shannon Index ◽  
Rhodiola Rosea ◽  
Pharmacologically Active ◽  
Cool Climate

Rhodiola rosea L. is a perennial adaptogenic medicinal plant found in cool climate of the northern hemisphere. The species is very diverse both in terms of morphological characteristics and in the content of the pharmacologically active substances. The genetic diversity of four geographically distant roseroot populations was studied with ISSR and SSR markers. Using 7 ISSR primers 64 DNA fragments were generated and 85,94% of those were found to be polymorphic, indicating high genetic variability at the species level (gene diversity = 0.33, Shannon index = 0.48). Lower level of diversity was detected at the population level (Shannon-index ranged from 0.2173 to 0.2696). Only four out of the eight SSR markers used were informative during this study. The primer pairs for these four SSR markers produced 25 fragments with an average of 6.25 putative alleles per locus. Observed heterozygosity ranged from 0.4 to 1.0, whereas expected heterozygosity ranged from 0.47 to 0.84. Cluster analysis based on both markers revealed the same groups, individuals clustered according to their geographic origin. The Southern-Uralian population was the most genetically isolated. ITS analysis was used for the determination whether these Southern-Uralian individuals belong to the same species.

scholarly journals Genetic diversity of maize inbred lines as inferred from SSR markers

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 489-498
Author(s):  
Ana Nikolic ◽  
Dragana Ignjatovic-Micic ◽  
Dragan Kovacevic ◽  
Zoran Camdzija ◽  
Milomir Filipovic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Principal Component ◽  
Inbred Lines ◽  
Yield Potential ◽  
Pedigree Data ◽  
Maize Breeding ◽  
Breeding Programs ◽  
Maize Inbred Lines ◽  
The World

Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and new problems related to different biotic and abiotic stresses and the growing needs of the world market require constant work on finding new ways for advancing maize production. Molecular marker technology is one of the fastest developing fields and its implementation has already given results in solving different problems related to maize breeding improvement. The aim of the study presented herein was characterization and genetic similarity assessment of twenty-nine maize inbred lines from Maize Research Institute collection using Simple Sequence Repeats (SSR) markers. The analysis was done using 20 pairs of SSR primers with clearly visible and reproducible results. A total of 119 alleles were detected with a mean of 5.8 per locus. PIC (Polymorphism Information Content) values were in the range from 0.45 to 0.92 (average 0.74). Genetic similarities calculated using Jaccard?s coefficient ranged from 0.27 to 0.99. Cluster and Principal Component Analysis (PCA) analysis were done using matrices of similarity in the NTSYSpc software, version 2.1. Results of both classifications were moderately in agreement with the pedigree data of analysed genotypes. The information about genetic diversity of maize inbred lines revealed by SSR markers could be useful in planning strategies for future maize breeding programs.

​SSR Based Genetic Diversity Analysis in Fenugreek (Trigonella foenum-graecum L.) Genotypes

2021 ◽  
Author(s):  
S.R. Maloo ◽  
Radheshyam Sharma ◽  
Himanshu Soan
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Trigonella Foenum Graecum ◽  
The World ◽  
Ctab Method ◽  
Medicinal Crop ◽  
Wide Variability ◽  
Trigonella Foenum Graecum L

Background: Fenugreek (Trigonella foenum-graecum L.) is an important seed spice crop widely grown all over the world. In India, the state of Rajasthan is known for fenugreek production and productivity in the world. A concerted assessment of genetic variability among the germplasm accession is essential for breeding new superior varieties. Molecular markers such as AFLP, RAPD, ISSR, SSR, SCAR, SCoT, SRAP have become for the characterization of the germplasm rapidly and accurately. The present study aimed to characterize 20 elite fenugreek genotypes using simple sequence repeat (SSR) markers to assess the existing genetic diversity of this medicinal crop. Methods: The present study was carried out at the Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan, India. Total genomic DNA was isolated from old leaves using the CTAB method (Doyle and Doyle, 1990). Further, PCR based genetic diversity was analyzed with using 50 SSR primer pairs. Dendrogram was constructed using NTSYSpc version 2.2 and clustering of the genotypes was done. Result: Twenty genotypes of fenugreek were assessed for genetic diversity analysis using SSR markers. Out of 50 markers 43 primer pairs produced 130 alleles with an average of 84.60% polymorphism. Jaccard’s similarity coefficient lied between and 0.39 to 0.82. Based on UPGMA clustering, a dendrogram consisting of five main clusters was generated with wide variability among the studied genotypes. These diverse genotypes so identified could be gainfully utilized in the fenugreek breeding programme.

scholarly journals Genetic diversity and relationship of Hunan province of China local tree peonies based on SSR markers

2019 ◽  
Vol 18 (4) ◽  
pp. 213-223
Author(s):  
Min-Huan Zhang ◽  
Xiao-Ling Jin ◽  
Ya-Feng Wen ◽  
Shouyun Shen ◽  
Wen Xing ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Shannon Index ◽  
Ornamental Plant ◽  
Hunan Province ◽  
Fixation Index ◽  
Tree Peony ◽  
The World ◽  
Relationship Of ◽  
Simple Sequence

Paeonia sect. Moutan is a wide known ornamental plant in the world. The objective of this study was to provide the theoretical basis for scientific preservation and utilization of tree peony resources of Hunan province of China. Simple sequence repeat (SSR) markers were applied to reveal the genetic diversity and relationship of 21 tree peony resources and 45 domestic and foreign tree peony cultivars. Clear bands, the size of which ranged from 115 bp to 379 bp, were detected with 14 primers. In total, 90 alleles were detected and the number of alleles detected with one primer varied between 5 and 13; the number of effective alleles ranged from 1.183 to 2.070; the polymorphism ratio of each locus was 100%. The observed heterozygosity, which ranged from 0.120 to 0.851 with an average of 0.532, was larger than the expected one, which ranged from 0.090 to 0.470 with an average of 0.300. Shannon index ranged from 0.137 to 0.695 and fixation index ranged from −0.332 to −0.869. The results show abundant genetic diversity in tree peony of Hunan province and SSR markers distinguishing homonymous tree peony resources successfully.

scholarly journals Development and Use of miRNA-derived SSR Markers to Study Genetic Diversity, Population Structure and Characterization of Genotypes for Heat Tolerance Breeding in Bread Wheat (Triticum aestivum L.)

2020 ◽  
Author(s):  
Sandhya Tyagi ◽  
Anuj Kumar ◽  
Tinku Gautam ◽  
Renu Pandey ◽  
Reyazul Rouf Mir
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Triticum Aestivum L ◽  
Breeding Programs ◽  
Mirna Genes ◽  
Novel Mirna ◽  
The World

AbstractWheat is one of the most important cereal crop in the world. Heat stress is an important abiotic stress limiting wheat production and productivity in the world including south-east Asia. The importance of miRNAs in gene expression under various biotic and abiotic stresses is well documented. Molecular markers, especially SSR markers, plays an important role for the success in molecular plant breeding programs. The discovery of SSRs from non-coding regions has been a challenging task. Therefore, development of novel miRNA-based SSRs from the conserved portions of the genome will prove useful for the study of genetic diversity of heat-responsive miRNA-genes in wheat. In the present study, efforts are made to mine SSR markers from 96 members of heat-responsive miRNA-genes of wheat followed by their validation using 37 contrasting (heat tolerance/susceptible) wheat genotypes. Among a set of 13 miRNA-SSRs used,7 SSRs were found polymorphic. Among these polymorphic SSR markers, three found to be very informative SSRs (HT-169j, HT-160a and HT-160b) and could largely discriminate heat tolerant genotypes from the heat susceptible ones. Further analysis based on Polymorphism Information Content (PIC) revealed that miRNA genes were more diverse in susceptible genotypes compared to tolerant genotypes. Ours is the first report that the genic/miRNA markers could be successfully used to study wheat diversity, population structure and characterization of trait specific germplasm. The important and useful miRNA-based SSRs, therefore, would serve as best markers in the marker-assisted breeding programs aimed at enhancing heat tolerance of Indian wheat.

scholarly journals Genetic divergence in basil cultivars and hybrids

2019 ◽  
Vol 37 (2) ◽  
pp. 180-187
Author(s):  
Rodrigo P Alves ◽  
Ana Veruska C Silva ◽  
Camila S Almeida-Pereira ◽  
Tatiana S Costa ◽  
Sheila V Alvares-Carvalho ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Variability ◽  
Polymorphic Information Content ◽  
Dna Amplification ◽  
Issr Markers ◽  
Economic Importance ◽  
Dissimilarity Index ◽  
The World ◽  
Issr Molecular Markers

ABSTRACT Basil is an aromatic herb that stands out for its economic importance. It is consumed in natura and used to obtain essential oil. The cultivation of this species in several regions of the world has allowed variations by natural crosses and euploidy, leading to the wide genetic variability found nowadays. Considering the importance of this species, we aimed to analyze the genetic diversity of 27 basil genotypes using ISSR molecular markers. Fourteen primers were employed for DNA amplification, resulting in 86% polymorphism. Based on the Jaccard’s dissimilarity index, the highest index (0.80) was observed between the individuals BAS001 and BAS012, while the lowest index (0.18) was detected between the genotypes BAS014 and BAS015. The genetic similarity among individuals was calculated, forming four distinct clusters. Most individuals (40.7%) were allocated in cluster I. The polymorphic information content (PIC) (0.89) indicated considerable levels of genetic diversity among genotypes. In this sense, the ISSR markers were efficient in the detection of polymorphisms between the accessions, suggesting the genetic variability of the collection. This result demonstrates the importance of the use of molecular markers and the advantages that this information provides to the breeding of the species.

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