scholarly journals Assessment of Allelopathic Potential of Senna garrettiana Leaves and Identification of Potent Phytotoxic Substances

Agronomy ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 139
Author(s):  
Ramida Krumsri ◽  
Arihiro Iwasaki ◽  
Kiyotake Suenaga ◽  
Hisashi Kato-Noguchi
Keyword(s):  
Ferulic Acid ◽  
Vanillic Acid ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
Lepidium Sativum ◽  
Allelopathic Potential ◽  
Natural Herbicides ◽  
The Individual ◽  
Spectral Data Analysis ◽  
Lepidium Sativum L

Senna garrettiana (Craib) Irwin & Barneby (Fabaceae) is a medicinal plant known to be rich in biologically active compounds that could be exploited to produce bioherbicides. The present study was conducted to explore the allelopathic potential and phytotoxic substances of S. garrettiana. Extracts of S. garrettiana leaves were found to significantly inhibit the growth of Lepidium sativum L. and Echinochloa crus-galli (L.) P. Beauv. (p ≤ 0.05). The phytotoxic substances were isolated and identified as vanillic acid and ferulic acid by bioassay-directed fractionation and spectral data analysis. The two compounds were shown to significantly inhibit the seed germination, seedling growth, and dry biomass of L. sativum. Based on the concentration required for 50% growth inhibition (defined as IC50), the roots of L. sativum were the most sensitive to the compounds, and the inhibitory effect of ferulic acid (IC50 = 0.62 mM) was >1.3 times more potent than that of vanillic acid (IC50 = 0.82 mM). In addition, a mixture of the two compounds (0.3 mM) resulted in synergistic inhibitory activity against the L. sativum roots compared with the individual compounds. These results suggest that the extracts of S. garrettiana leaves and their phytotoxic compounds have potential as candidate natural herbicides.

scholarly journals Isolation and Identification of Two Potent Phytotoxic Substances from Afzelia xylocarpa for Controlling Weeds

2021 ◽  
Vol 11 (8) ◽  
pp. 3542
Author(s):  
Ramida Krumsri ◽  
Kaori Ozaki ◽  
Toshiaki Teruya ◽  
Hisashi Kato-Noguchi
Keyword(s):  
Ferulic Acid ◽  
Vanillic Acid ◽  
Synergistic Effects ◽  
Phleum Pratense ◽  
Lepidium Sativum ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Isolation And Identification ◽  
Phytotoxic Activity ◽  
Seedling Length

Phytotoxic substances released from plants are considered eco-friendly alternatives for controlling weeds in agricultural production. In this study, the leaves of Afzelia xylocarpa (Kurz) Craib. were investigated for biological activity, and their active substances were determined. Extracts of A. xylocarpa leaf exhibited concentration-dependent phytotoxic activity against the seedling length of Lepidium sativum L., Medicago sativa L., Phleum pratense L., and Echinochloa crus-galli (L.) P. Beauv. Bioassay-guided fractionation of the A. xylocarpa leaf extracts led to isolating and identifying two compounds: vanillic acid and trans-ferulic acid. Both compounds were applied to four model plants using different concentrations. The results showed both compounds significantly inhibited the model plants’ seedling length in a species-dependent manner (p < 0.05). The phytotoxic effects of trans-ferulic acid (IC50 = 0.42 to 2.43 mM) on the model plants were much greater than that of vanillic acid (IC50 = 0.73 to 3.17 mM) and P. pratense was the most sensitive to both compounds. In addition, the application of an equimolar (0.3 mM) mixture of vanillic acid and trans-ferulic acid showed the synergistic effects of the phytotoxic activity against the root length of P. pratense and L. sativum. These results suggest that the leaves of A. xylocarpa and its phytotoxic compounds could be used as a natural source of herbicides.

scholarly journals Influence of drought on allelopathic properties of Pinus sylvestris L.

2020 ◽  
Vol 85-86 ◽  
Author(s):  
N.V. Rositska
Keyword(s):  
Soil Moisture ◽  
Pinus Sylvestris ◽  
Shoot Elongation ◽  
Botanical Garden ◽  
Inhibitory Effect ◽  
Lepidium Sativum ◽  
Allelopathic Effect ◽  
Natural Ecosystems ◽  
Allelopathic Potential ◽  
Allelopathic Activity

Objective – to investigate the influence of drought on allelopathic activity of Pinus sylvestris L. plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. The choice of study objects was due to their habitat: plants of P. sylvestris grew in compliance with the necessary agricultural technology including regular irrigation at site 1 (control) and in the conditions of drought at site 2 that models natural ecosystems (experiment). The allelopathic activity of leaves and soil was determined. In the model experiments, Lepidium sativum L. ‘Azhur’, Triticum aestivum L. ‘Poliska 90’, Amaranthus caudatum L. ‘Rothschwanz’ were applied as the test plants. Results of the current study revealed that the allelopathic potential of needles and rhizosphere soil of P. sylvestris depends not only from the species but also from drought conditions. The inhibitory effect was stronger against root elongation than shoot elongation of seedlings. Conclusion. This study provides a laboratory-based evidence of the allelopathic effect of P. sylvestris exudates under drought stress on model plants. Studies showed that plants grown under conditions of lower soil moisture exhibit stronger inhibitory effect or slightly stimulate the growth of test objects in comparison with plants that had better water supply. This testifies to the relationship between soil moisture and seasonal allelopathic activity of the plants.

scholarly journals Garcienone, a Novel Compound Involved in Allelopathic Activity of Garcinia Xanthochymus Hook

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 301 ◽  
Author(s):  
Md. Mahfuzur Rob ◽  
Arihiro Iwasaki ◽  
Ryota Suzuki ◽  
Kiyotake Suenaga ◽  
Hisashi Kato-Noguchi
Keyword(s):  
Inhibitory Activity ◽  
Methanol Extract ◽  
Lepidium Sativum ◽  
Aqueous Methanol ◽  
Allelopathic Potential ◽  
Barnyard Grass ◽  
Garcinia Xanthochymus ◽  
Medicago Sativa L ◽  
Promising Source ◽  
Lepidium Sativum L

Plants are sources of diversified allelopathic substances that can be investigated for use in eco-friendly and efficient herbicides. An aqueous methanol extract from the leaves of Garcinia xanthochymus exhibited strong inhibitory activity against barnyard grass (Echinochloa crus-galli (L.) P. Beauv.), foxtail fescue (Vulpia myuros (L.) C.C.), alfalfa (Medicago sativa L.), and cress (Lepidium sativum L.), and appears to be a promising source of allelopathic substances. Hence, bio-activity guided purification of the extract through a series of column chromatography steps yielded a novel compound assigned as garcienone ((R, E)-5-hydroxy-5-((6S, 9S)-6-methyl-9-(prop-13-en-10-yl) tetrahydrofuran-6-yl) pent-3-en-2-one). Garcienone significantly inhibited the growth of cress at a concentration of 10 μM. The concentrations resulting in 50% growth inhibition (I50) of cress roots and shoots were 120.5 and 156.3 μM, respectively. This report is the first to isolate and identify garcienone and to determine its allelopathic potential.

scholarly journals Assessment of some medicinal plants for their allelopathic potential against redroot pigweed (Amaranthus retroflexus)

2014 ◽  
Vol 54 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Marzieh Sabagh Nekonam ◽  
Jamshid Razmjoo ◽  
Hasan Kraimmojeni ◽  
Bahram Sharifnabi ◽  
Hajar Amini ◽  
...  
Keyword(s):  
Ricinus Communis ◽  
Dry Weight ◽  
Inhibitory Effect ◽  
Amaranthus Retroflexus ◽  
Crocus Sativus ◽  
Nerium Oleander ◽  
Aqueous Extracts ◽  
Allelopathic Potential ◽  
Natural Herbicides ◽  
Redroot Pigweed

Abstract The study was conducted to determine the allelopathic effects of Crocus sativus L., Ricinus communis L., Nicotiana tabacum L., Datura inoxia Mill., Nerium oleander L., and Sorghum vulgare L. on the germination and growth of Amaranthus retroflexus (redroot pigweed). Powder and aqueous extracts of these plants were used to run the experiment under laboratory and greenhouse conditions. In the laboratory, all aqueous extracts showed a significant inhibitory effect on the germination, seedling length and weight of redroot pigweed plants. The most allelopathic against the redroot pigweed were R. communis, N. tabacum, and D. inoxia. In the greenhouse experiment, extracts and the powder of these plants also showed significant inhibitory effects on pigweed dry weight, height, leaf area, number of survivor plants, and amount of chlorophyll. In the germination bioassay and application of powder, the inhibitory effect was dosage dependent - the higher the concentration, the strongest the inhibitory effect. From the obtained results, it can be concluded that the powder and extracts of the tested species have an herbicidal potential against redroot pigweed and could be used as natural herbicides and mulches.

Purification and Characterization of Lupus Anticoagulant Like Protein from Agkistrodon Halys Brevicaudus Venom

1996 ◽  
Vol 76 (06) ◽  
pp. 0993-0997
Author(s):  
Zhao-Yan Li ◽  
Xiao-Wei Wu ◽  
Tie-Fu Yu ◽  
Eric C-Y Lian
Keyword(s):  
Amino Acid ◽  
Lupus Anticoagulant ◽  
Inhibitory Effect ◽  
Clotting Factor ◽  
Acid Oxidase ◽  
Crude Venom ◽  
Amino Acid Oxidase ◽  
Sds Page ◽  
The Individual ◽  
Reducing Condition

SummaryBy means of CM-Sephadex C-25, DEAE-Sephadex A-50, Sephadex G-200, and Sephadex G-75 chromatographies, a lupus anticoagulant like protein (LALP) from Agkistrodon halys brevicaudus was purified. On SDS-PAGE, the purified LALP had a molecular weight of 25,500 daltons under non-reducing condition and 15,000 daltons under reducing condition. The isoelectric point was pH 5.6. Its N terminal amino acid sequencing revealed a mixture of 2 sequences: DCP(P/S)(D/G)WSSYEGH(C/R)Q(Q/K). It was devoid of phospho-lipaseA, fibrino(geno)lytic, 5′-nucleotidase, L-amino acid oxidase, phosphomonoesterase, phosphodiesterase and thrombin-like activities, which were found in crude venom. In the presence of LALP, PT, aPTT, and dRVVT of human plasma were markedly prolonged and its effects were concentration-dependent but time-independent. The inhibitory effect of LALP on the plasma clotting time was enhanced by decreasing phospholipid concentration in TTI test. The individual clotting factor activity was not affected by LALP when higher dilutions of LALP-plasma mixture were used for assay. Russell’s viper venom time was shortened when high phospholipid confirmatory reagent was used. Therefore, the protein has lupus anticoagulant property.

scholarly journals Combined Luteolin and Indole-3-Carbinol Synergistically Constrains ERα-Positive Breast Cancer by Dual Inhibiting Estrogen Receptor Alpha and Cyclin-Dependent Kinase 4/6 Pathway in Cultured Cells and Xenograft Mice

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2116
Author(s):  
Xiaoyong Wang ◽  
Lijuan Zhang ◽  
Qi Dai ◽  
Hongzong Si ◽  
Longyun Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cell ◽  
Breast Cancer Cells ◽  
Cultured Cells ◽  
Inhibitory Effect ◽  
Cyclin Dependent Kinase ◽  
Xenograft Mice ◽  
The Individual

The high concentrations of individual phytochemicals in vitro studies cannot be physiologically achieved in humans. Our solution for this concentration gap between in vitro and human studies is to combine two or more phytochemicals. We screened 12 phytochemicals by pairwise combining two compounds at a low level to select combinations exerting the synergistic inhibitory effect of breast cancer cell proliferation. A novel combination of luteolin at 30 μM (LUT30) and indole-3-carbinol 40 μM (I3C40) identified that this combination (L30I40) synergistically constrains ERα+ breast cancer cell (MCF7 and T47D) proliferation only, but not triple-negative breast cancer cells. At the same time, the individual LUT30 and I3C40 do not have this anti-proliferative effect in ERα+ breast cancer cells. Moreover, this combination L30I40 does not have toxicity on endothelial cells compared to the current commercial drugs. Similarly, the combination of LUT and I3C (LUT10 mg + I3C10 mg/kg/day) (IP injection) synergistically suppresses tumor growth in MCF7 cells-derived xenograft mice, but the individual LUT (10 mg/kg/day) and I3C (20 mg/kg/day) do not show an inhibitory effect. This combination synergistically downregulates two major therapeutic targets ERα and cyclin dependent kinase (CDK) 4/6/retinoblastoma (Rb) pathway, both in cultured cells and xenograft tumors. These results provide a solid foundation that a combination of LUT and I3C may be a practical approach to treat ERα+ breast cancer cells after clinical trials.

Toxicological effects of different-sized Co–Fe (CoFe2O4) nanoparticles on Lepidium sativum L.: towards better understanding of nanophytotoxicity

Ecotoxicology ◽  
2021 ◽  
Vol 30 (2) ◽  
pp. 277-291
Author(s):  
D. Montvydienė ◽  
A. Jagminas ◽  
Ž. Jurgelėnė ◽  
M. Kazlauskas ◽  
R. Butrimienė ◽  
...  
Keyword(s):  
Lepidium Sativum ◽  
Cofe2o4 Nanoparticles ◽  
Toxicological Effects ◽  
Lepidium Sativum L

scholarly journals Phytotoxicity of Tires Evaluated in Simulated Conditions

Environments ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 49
Author(s):  
Markéta Šourková ◽  
Dana Adamcová ◽  
Jan Winkler ◽  
Magdalena Daria Vaverková
Keyword(s):  
Higher Plants ◽  
Sinapis Alba ◽  
Lepidium Sativum ◽  
Root Growth Inhibition ◽  
Chemical Parameters ◽  
Sinapis Alba L ◽  
Test Kit ◽  
Physical And Chemical ◽  
Aqueous Leachate ◽  
Lepidium Sativum L

Illegal dumps and landfills with disposed of tires are a fact of today, which should not be neglected as they represent a great ecological burden for the environment, affect the surrounding nature and disturb the landscape. This research was focused on testing the phytotoxicity of aqueous leachates from the fractions of tires in two sets of experiments—to simulate laboratory conditions (tire leaching in distilled water) and natural conditions (tire leaching in water from a recipient) using the Phytotoxkit testing kit (kit for the establishment of inhibition/stimulation effect on the root development) and the watercress test of phytotoxicity (biological method for the assessment of leachate phytotoxicity). Plants whose seeds were selected for the test were watercress (Lepidium sativum L.) and white mustard (Sinapis alba L.). The aqueous leachate was tested for 38 weeks. During the experiment, physical and chemical parameters were measured at intervals of 14 days by the testing instrument HACH TEST KIT: electric conductivity (EC), amount of dissolved oxygen (LDO) and pH. Results of root growth inhibition (IR) on the seeds of Lepidium sativum L. and Sinapis alba L. exhibited values ranging from 11.73% to 47.74% in the tested samples. Results of germination index (GI) on the seeds of Lepidium sativum L. exhibited values below 66% in the tested samples, which indicated the leachate phytotoxicity. In spite of the fact that similar studies are tackling the acute toxicity of leachates from tires (particularly to algae, embryos and animals), this research brings complementary information in testing the acute phytotoxicity of tire leachates to higher plants.

scholarly journals Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 19
Author(s):  
Anastasia Zerva ◽  
Nikolaos Tsafantakis ◽  
Evangelos Topakas
Keyword(s):  
Biomass Conversion ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
White Rot ◽  
Oxidative Enzymes ◽  
Industrial Residues ◽  
Pure Compounds ◽  
Wild Strains

White-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cellulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important application in cosmetic formulations, either as pure compounds or as standardized extracts. In the present work, three wild strains of Basidiomycetes fungi (Pleurotus citrinopileatus, Abortiporus biennis and Ganoderma resinaceum) from Greek habitats were grown in agro-industrial residues (oil mill wastewater, and corn cob) and evaluated for their anti-tyrosinase and antioxidant activity and for the production of biotechnologically relevant enzymes. P. citrinopileatus showed the most interesting tyrosinase inhibitory activity, while A. biennis showed the highest DPPH(2,2-diphenyl-1-picryl-hydrazyl) scavenging potential. Corn cobs were the most appropriate carbon source for maximizing the inhibitory effect of fungal biomasses on both activities, while the use of oil mill wastewater selectively increased the anti-tyrosinase potential of P. citrinopileatus culture filtrate. All strains were found to be preferential lignin degraders, similarly to most white-rot fungi. Bioinformatic analyses were performed on the proteome of the strains P. citrinopileatus and A. biennis, focusing on CAZymes with biotechnological relevance, and the results were compared with the enzyme activities of culture supernatants. Overall, all three strains showed strong production of oxidative enzymes for biomass conversion applications.

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