scholarly journals Effect of protease supplementation on the digestibility of amino acids in animal-origin meals for broiler diets

2021 ◽  
Vol 66 (No. 1) ◽  
pp. 29-37
Author(s):  
Júlia Marixara Sousa da Silva ◽  
Nayanne Rodrigues de Oliveira ◽  
Alison Batista Vieira Silva Gouveia ◽  
Rodolfo Alves Vieira ◽  
Ronan Omar Fernandes dos Santos ◽  
...  
Keyword(s):  
Amino Acids ◽  
Nitrogen Balance ◽  
Broiler Chickens ◽  
Nutrient Digestibility ◽  
Metabolizable Energy ◽  
Animal Origin ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Apparent Metabolizable Energy

Enzymes benefit digestion and absorption of the ingredients and their addition to an animal-origin meal (AOM) can improve its nutritional quality. This research aimed to evaluate the effect of protease on nutrient digestibility, amino acids, and metabolism of AOM energy for broilers. Four hundred and eighty broiler chickens were distributed in a completely randomized design (4 × 2 factorial scheme), eight treatments, six replicates containing 10 birds/replicate. Treatments consisted of poultry viscera meal, swine viscera meal (SVM), bovine meat and bone meal, and basal diet; with and without protease addition. Two tests were performed. In the first test, the total excreta collection method was used with birds at 13 to 20 days of age and 25% of the reference feed was replaced by AOM. In the second test, a protein-free diet was administered to birds at 21 to 24 days of age and AOM replaced 25% of the starch. The inclusion of protease increased the apparent metabolizable energy corrected for nitrogen balance of SVM by 15.99% and the apparent metabolizable crude energy by 5.7%, and it also raised the coefficient of true ileal digestibility of the amino acids in the AOMs by 5.67% on average. The inclusion of protease improved the apparent metabolizable crude energy of AOMs, apparent metabolizable dry matter of bovine meat and bone meal, coefficient of true ileal digestibility of essential amino acids, and apparent metabolizable energy corrected for nitrogen balance of SVM. Dietary supplementation of protease may be a potential strategy to improve the digestibility of amino acids for broilers, a possibility of using animal-origin meals as a protein source of diets.

Comparison of the Ileal Digestibility of Amino Acids in Meat and Bone Meal for Broiler Chickens and Growing Rats

2005 ◽  
Vol 4 (4) ◽  
pp. 192-196 ◽  
Author(s):  
V. Ravindran . ◽  
W.H. Hendriks . ◽  
D.V. Thomas . ◽  
P.C.H. Morel . ◽  
C.A. Butts .
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Growing Rats

scholarly journals Effects of Fermentation on Standardized Ileal Digestibility of Amino Acids and Apparent Metabolizable Energy in Rapeseed Meal Fed to Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1774
Author(s):  
Zhengke Wu ◽  
Jiao Liu ◽  
Jiang Chen ◽  
Shoaib Ahmed Pirzado ◽  
Yang Li ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Nutritive Value ◽  
Animal Feed ◽  
Basal Diet ◽  
Rapeseed Meal ◽  
Metabolizable Energy ◽  
Isoleucine Leucine ◽  
Ileal Digestibility ◽  
Apparent Metabolizable Energy

Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher (p < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.

scholarly journals Metabolizable energy and digestible amino acids of full-fat soybean without or with protease supplementation in diets for broilers

2016 ◽  
Vol 40 (5) ◽  
pp. 565-576 ◽  
Author(s):  
Felipe Santos Dalólio ◽  
Luiz Fernando Teixeira Albino ◽  
Horácio Santiago Rostagno ◽  
Diego Ladeira da Silva ◽  
Maurílio de Lucas Xavier Júnior ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Control Diet ◽  
Amino Acid Content ◽  
Metabolizable Energy ◽  
Broiler Chicks ◽  
Ileal Digestibility ◽  
Apparent Metabolizable Energy ◽  
Protein Free Diet ◽  
Digestible Amino Acids

ABSTRACT Two experiments were carried out to determine the energy values and the true ileal digestibility of amino acids of nine toasted full-fat soybeans (FFSB), without or with the addition of monocomponent protease. In the first experiment, to determine the energy values, the method of excreta collection was used with 1120 broiler chicks at 14 days old, distributed in randomized blocks in a 10x2 factorial arrangement with nine full-fat soybeans (30% included) plus a control diet (70%) without or with the addition of protease, totaling 20 treatments with 8 replicates and 7 birds per cage. In the second experiment, 1120 broiler chickens from 24 to 28 days of age were distributed in randomized blocks in a 10x2 factor arrangement, with nine full-fat soybeans (40% included) + a protein free diet (60%), without or with the addition of protease, totaling 20 treatments with 8 replicates and 7 birds per cage. Ileal digesta collection was used to determine the true digestibility coefficients and the digestible amino acid content of full-fat soybean. The average values of nitrogen-corrected apparent metabolizable energy (AMEn) and their respective metabolization coefficients (CAMEn) were 3207 kcal/kg and 62.57%, respectively. The average values of digestibility coefficients of crude protein and essential and nonessential amino acids were 86.79, 87.90 and 84.34%, respectively. The inclusion of protease improved (P<0.05) all evaluated parameters. Therefore, its use is recommended in diets containing full-fat soybeans for broiler chickens.

scholarly journals Energetic values of animal by-products for broiler chickens of different ages

2021 ◽  
Vol 10 (6) ◽  
pp. e37110615639
Author(s):  
José Luiz Schneiders ◽  
André Sanches de Avila ◽  
Jomara Broch ◽  
Rafael Frank ◽  
Rodrigo André Schöne ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Broiler Chickens ◽  
Metabolizable Energy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Linear Effect ◽  
Poultry Fat ◽  
Different Ages ◽  
Apparent Metabolizable Energy ◽  
By Products

The objective of this study was to determine the chemical composition and apparent metabolizable energy (AME), AME corrected for nitrogen balance (AMEn) and its respective metabolizable coefficients of animal byproducts for broiler chickens with different ages. Meat and bone meal, poultry by-product meal, tilapia processing residue and poultry fat were evaluated. A total of 760 male broiler chickens were used and evaluated and the phases: pre-starter (1 to 8 d of age); starter (11 to18 d); grower 1 (21 to 28 d); grower 2 (31 to 38 d), and finisher (41 to 48 d). Total excreta collection method was performed in five metabolism assays. The experimental design was completely randomized, and data were submitted to analysis of variance, posteriorly, the four feeds were compared by Tukey test and a regression analysis was performed with broiler chickens age. The significance was considered at 5% probability. The values of AME and AMEn were higher in older birds for all ingredients. The lesser CAME and CAMEn were obtained for meat and bone meal, for the regression analysis poultry by-product meal, tilapia processing residue and poultry fat had an increased linear effect with birds age and there was no adjust for meat and bone meal for regression analysis.

Standardized ileal digestibility of amino acids and apparent metabolizable energy in corn and soybean meal for organic broiler chicken production in Ontario

2020 ◽  
Vol 100 (3) ◽  
pp. 447-454 ◽  
Author(s):  
Haley Leung ◽  
Elijah G. Kiarie
Keyword(s):  
Amino Acids ◽  
Soybean Meal ◽  
Broiler Chickens ◽  
Metabolizable Energy ◽  
Wheat Starch ◽  
Energy Utilization ◽  
Ileal Digestibility ◽  
Feed Formulation ◽  
Apparent Metabolizable Energy ◽  
Broiler Feed

Standardized ileal digestibility (SID) of amino acids (AA) and apparent metabolizable energy corrected for nitrogen (AMEn) in samples of organic corn and soybean meal (SBM) were determined. Conventional corn (CC) and SBM (CSBM) samples were tested for comparison. A total of 560, fourteen-day-old male broiler chickens (Cobb 500) were weighed, placed in cages (10 birds per cage), and allocated to seven (n = 8) semi-purified wheat-starch-based diets. Diets were (1) CC, (2) imported organic corn, (3) local organic corn, (4) CSBM, (5) imported organic SBM (OSBMI), (6) local organic SBM (OSBML), and (7) nitrogen-free wheat starch. Only few differences were observed on SID of AA; SID of lysine was lower (P = 0.002) in organic corn samples relative to CC, and SID of methionine was lower (P = 0.002) in OSBML sample relative to CSBM and OSBMI samples. The AMEn of CC was higher (P < 0.01) than that of organic corn samples. The AMEn of OSBML was higher (P < 0.001) than for CSBM and OSBMI; however, the value for OSBMI was higher (P < 0.001) than for CSBM. In conclusion, utilization of AA in conventional and organic feedstuffs was comparable; however, differences in energy utilization warrant considerations in organic broiler feed formulation.

scholarly journals 118 Digestibility of amino acids in hydrolyzed feather meal, flash dried poultry protein, poultry meal, and meat and bone meal fed to broiler chickens and pigs

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 65-65
Author(s):  
Chan Sol Park ◽  
Victor Naranjo ◽  
Ariane Helmbrecht ◽  
John K Htoo ◽  
Olayiwola Adeola
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Block Design ◽  
Latin Square ◽  
Experimental Period ◽  
Meat And Bone Meal ◽  
Feather Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Randomized Complete Block Design

Abstract This study was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in hydrolyzed feather meal (HFM), flash dried poultry protein (FDPP), poultry meal (PM), and meat and bone meal (MBM) fed to broiler chickens and pigs. Four semi-purified diets and a nitrogen-free diet were prepared. Exp. 1 was conducted with 416 male broiler chickens (initial body weight [BW] = 705 ± 100.0 g) at d 18 post-hatching in a randomized complete block design. Birds were fed diets for 5 d and then euthanized to collect ileal digesta. In Exp. 2, ten barrows (initial BW = 22.1 ± 1.59 kg) surgically fitted with T-cannulas at the distal ileum were assigned to duplicate 5 × 4 incomplete Latin square design with 5 diets and 4 periods. There were 5-d adaptation and 2-d ileal digesta collection periods in each experimental period. Data were analyzed as a 2 × 4 factorial arrangement with the effects of species (i.e., broiler chickens and pigs) and four experimental diets. There were interactions (P < 0.05) in the SID of His, Lys, Thr, Trp, and Val between diets and species. In broiler chickens, the SID of Lys in MBM was greater (P < 0.05) than HFM and FDPP but was not different from PM. Pigs fed MBM had greater (P < 0.05) SID of Lys than HFM, FDPP, and PM. In broiler chickens, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was less (P < 0.05) than MBM. In pigs, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was not different from MBM. In conclusion, differences in the SID of AA among animal protein sources were affected by species of monogastric animals.

scholarly journals Effect of Extrusion on Nutrients Digestibility, Metabolizable Energy and Nutritional Value of Yellow Lupine Seeds for Broiler Chickens

2016 ◽  
Vol 16 (4) ◽  
pp. 1059-1072 ◽  
Author(s):  
Andrzej Rutkowski ◽  
Sebastian A. Kaczmarek ◽  
Marcin Hejdysz ◽  
Dorota Jamroz
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Nutritional Value ◽  
Nitrogen Retention ◽  
Metabolizable Energy ◽  
Ileal Digestibility ◽  
Positive Effects ◽  
Yellow Lupine ◽  
Dietary Treatments ◽  
Fat Digestibility

Abstract The aim of investigations was the estimation of nutritional value of currently cultivated yellow lupine cultivars in raw and extruded form, and their usefulness for broiler chickens. Two experiments were conducted with male Ross 308 chickens. In a digestibility trial 60 fifteen-day-old birds were randomly assigned to three dietary treatments (20 replications in each). Birds were kept in individual pens. Digestibility was calculated using the difference method. From day 16 to 21, diets contained lupine meal in raw or extruded form and the basal diet in the ratio 20:80. Subsequently, the total digestibility of dry matter and crude fat, also apparent nitrogen retention and AMEN value of lupine seeds were determined on chickens fed different forms of lupine. The ileal digestibility of crude protein and amino acids of lupine seeds was also analyzed. In the second experiment the one-day-old birds were randomly assigned to ten dietary treatments (10 replications in each) and were fed diets with increasing amounts of raw or extruded lupine from 10 to 30%. The chickens in control treatments were fed a diet without lupine. Extruded yellow lupine meal was characterized by lower phytic P content in comparison to raw yellow lupine meal. The content of remaining ingredients and antinutritional substances was similar. Yellow lupine seeds post extrusion were characterized by better total crude fat digestibility, nitrogen retention and AMEN values, compared to raw seeds. Higher ileal digestibility was confirmed in numerous amino acids, except lysine, threonine and valine (P≤0.05). By feeding the broilers with diets consisting of 10 to 30% of lupine seeds post extrusion (experiment II), improved apparent fat digestibility, apparent nitrogen retention and AMEN values were achieved in young chickens (P<0.01). Using 10 and 20% of lupine in the diets showed significant positive effects of extrusion on body weight gains, feed intake and feed conversion rate. The performance indices of chickens were drastically decreased by use of 25% ratio of both raw and extruded yellow lupine in the diet. This effect was heightened by a 30% share in feed mixtures.

scholarly journals The effect of dietary crude protein content on apparent and true ileal nitrogen and amino acid digestibilities

1994 ◽  
Vol 72 (1) ◽  
pp. 59-68 ◽  
Author(s):  
A. Donkoh ◽  
P. J. Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Crude Protein ◽  
Apparent Digestibility ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Dietary Protein Level

The effect of dietary protein content (25, 60, 95, 130, 165 and 200 g crude protein (N × 6.25)/kg diet) on the apparent and true ileal digestibilities of N and amino acids in meat-and-bone meal given to the growing rat was investigated. Semi-synthetic diets in which meat-and-bone meal was the sole protein source were given to 180 g body-weight rats for 14 d. On the fourteenth day the rats were fed and then killed 4 h after the start of feeding and digesta were sampled from the terminal 200 mm ileum. Endogenous amino acid excretion was determined for eighteen rats given an enzymically hydrolysed casein (EHC)-based diet and with subsequent treatment of the digesta using ultrafiltration. The EHC-fed rats were killed 3 h after the start of feeding and digesta were collected from the terminal 200 mm ileum. True ileal digestibility values determined with reference to Cr as a marker were higher than the corresponding apparent estimates. Apparent digestibility values of N and amino acids increased significantly (P < 0.001) with increasing dietary protein level; however, dietary protein content had no significant (P > 0.05) effect on the true ileal digestibilities of N and amino acids. The mean apparent ileal digestibility of N in meat-and-bone meal ranged from 65.6 to 75.3%. The corresponding range for the true ileal digestibility of N was 76.9 to 78.2%. True ileal digestibility, unlike apparent digestibility, appears to be independent of dietary protein level and may allow feed ingredients to be compared accurately even if they are ingested in different quantities.

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