Milk Production Responses and Digestibility of Dairy Buffaloes (Bubalus bubalis) Partially Supplemented with Forage Rape (Brassica Napus) Silage Replacing Corn Silage

Worldwide, silage is considered the main component in dairy animal diets; however, this portion is mainly dominated by corn silage, which raises availability challenges in some agricultural production systems. The present study evaluated a partial replacement of corn silage with forage rape silage (FRS) and its effect on feed intake, nutrient digestibility, rumen fermentation, milk production, and blood metabolites in buffalo. Thirty-six lactating buffaloes were randomly assigned to four different groups, according to supplementation of FRS (only corn silage, FRS0) or with 15% (FRS15), 25% (FRS25), and 35% (FRS35) of forage rape silage instead of corn silage. The results showed that, compared to corn silage, forage rape silage has a lower carbohydrate but a higher protein concentration. The buffalo intake of dry matter and organic matter were improved linearly with the FRS increasing in the diet. The apparent total-tract digestibility (ATTD) of dry matter, organic matter, nitrogen, neutral detergent fiber, and acid detergent fiber also increased by the FRS supplementation compared with FRS0. Conversely, FRS supplementation decreased the propionic, butyric, and valeric acid contents and increased the acetic:propionic ratio and microbial protein content. Furthermore, FRS inclusion led to a significantly higher milk urea and non-fat milk solid content, higher blood glucose, total globulins, blood urea nitrogen, and lower blood high-density lipoprotein. These results suggested that FRS has high a nutritional value and digestibility, is a good feed resource, and showed favorable effects when supplemented with dairy buffalo ration.

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Feed intake, nutrient digestibility, milk production and composition in dairy cows fed silage of wet brewers grain

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n2p1069 ◽

2016 ◽

Vol 37 (2) ◽

pp. 1069 ◽

Cited By ~ 2

Author(s):

Leiliane Cristine de Souza ◽

Maximiliane Alavarse Zambom ◽

Claudete Regina Alcalde ◽

Tatiane Fernandes ◽

Deise Dalazen Castagnara ◽

...

Keyword(s):

Organic Matter ◽

Milk Production ◽

Wheat Bran ◽

Dry Matter ◽

Milk Composition ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Holstein Cows ◽

Soybean Hulls ◽

Ground Corn

The aim of this study was to evaluate the supply of wet brewers grain silage with and without nutrient additives in ensiling (wheat bran, soybeans hulls and ground corn), and the effect of additives on the intake and digestibility of dry matter and nutrients as well as the efficiency, production and milk composition of Holstein cows. Four multiparous Holstein cows were used, with an average milk production of 25 liters per day. The cows were randomly assigned, via the Latin Square design (4x4), to one of four experimental diets. The experimental period was 21 days. The results obtained in the experiment were evaluated by analysis of variance, Fisher’s F test and Tukey’s test at 5 % probability. The experimental diets consisted of wet brewers grain silage alone, and this silage with the addition of wheat bran, soybean hulls or ground corn. The diets did not affect the initial body weight of the animals. Diets containing wheat bran and ground corn provided a higher intake of dry matter (21.78 and 21.50 kg day-1) and organic matter (20.42 and 20.22 kg day-1) compared to the wet brewers grain silage alone and the diet containing soybean hulls. The results observed for dry matter (636.96 and 632.96 g kg-1), organic matter (659.16 and 654.35 g kg-1 DM), crude protein (712.84 and 717.79 g kg-1 DM) and neutral detergent fiber (598.99 and 538.90 g kg-1 DM) when cows were fed diets containing soybean hulls and ground corn, respectively, were higher than other diets. The different diets did not change daily milk production, milk production corrected for 3.5 % fat or milk composition. The milk urea nitrogen from cows fed ground corn diets was a lower concentration (17.67 mg dL-1), however, it was still above the suitable range (10 to 14 mg dL-1). Providing wet brewers grain silage with soybeans hulls or ground corn to Holstein cows increased the digestibility of dry matter and nutrients. However, the use of silages with wheat bran, soybean hulls or ground corn did not alter the efficiency, production or milk composition of cows fed with these silages.

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Replacement of raw soybean with roasted soybean increased milk production in Holstein cows

Ciência Rural ◽

10.1590/0103-8478cr20160002 ◽

2017 ◽

Vol 47 (5) ◽

Cited By ~ 1

Author(s):

Gilson Sebastião Dias Júnior ◽

Vítor Augusto Silveira ◽

Ivan Júnior Ascari ◽

Renata Apocalypse Nogueira Pereira ◽

Marina de Arruda Camargo Danés ◽

...

Keyword(s):

Milk Production ◽

Dry Matter ◽

Degradation Kinetics ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Production Performance ◽

Holstein Cows ◽

Lower Protein ◽

Inclusion Level ◽

Improved Performance

ABSTRACT: The aim of this study was to evaluate the effect of total replacement of raw whole soybean (RAW) for roastedwhole soybean (ROS) on the production performance of Holstein cows. Two experiments were carried out usinga simple reversal design where RAW has been completely replaced by ROS. In experiment 1, 22 cows (175±60 days in milk)were used, and the dietary inclusion level of RAW or ROS was 3.7% of dry matter (DM). In experiment 2, 16 cows (130±50 days in milk)were used, and thedietary inclusion level of RAW or ROS was 11% of DM. In both experiments, ROS increased milk production by 1.1kgday-1 without changing fat and protein production. Dry matter intake or milk urea nitrogenwere not affected by dietary soy source. In experiment 2, plasma glucose concentration was decreased, and allantoin/creatinine ratio in urine tended to decreasein ROS. Experiment 2 also evaluated the nutrient digestibility and ruminal degradation kinetics of crude protein in two soybean sources. Roasting had no effect on the digestibility of DM, organic matter, and neutral detergent fiber. Roasted whole soybean hadgreater fraction B and lower protein degradation rate than did RAW; this showed that heat treatment was effective in increasing therumen undegradable amino acid flowto the animal, which suggesteda potential mechanism of action for improved performance observed in ROS.

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Effects of source and concentration of neutral detergent fiber from roughage in beef cattle diets on feed intake, ingestive behavior, and ruminal kinetics

Journal of Animal Science ◽

10.1093/jas/skaa107 ◽

2020 ◽

Vol 98 (5) ◽

Author(s):

Rodrigo S Goulart ◽

Ricardo A M Vieira ◽

Joao L P Daniel ◽

Rafael C Amaral ◽

Vanessa P Santos ◽

...

Keyword(s):

Beef Cattle ◽

Feed Intake ◽

Dry Matter ◽

Latin Square ◽

Nutrient Digestibility ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Ingestive Behavior ◽

Rumen Content ◽

Residence Times

Abstract The objectives of this research were to evaluate the effects of source and concentration of α-amylase-treated neutral detergent fiber (aNDF) from roughage on feed intake, ingestive behavior, and ruminal kinetics in beef cattle receiving high-concentrate diets. Six ruminally cannulated Nellore steers (408 ± 12 kg of body weight) were randomly assigned to a 6 × 6 Latin square design with six diets: 10% aNDF from corn silage (10CS); 20% aNDF from corn silage (20CS); or four diets containing 10% aNDF from corn silage and 10% aNDF from one of the following sources: sugarcane (SC), sugarcane bagasse (SCB), soybean hulls (SH), or low oil cottonseed hulls (LOCH). The parameters of passage and degradation kinetics were estimated based on a two-compartmental model with gamma- and exponential-distributed residence times. The nonlinear models were fitted by nonlinear least squares, and a linear mixed-effects model was fitted to all variables measured from the Latin square design that were related to intake, digestibility, digestion kinetic parameters, and residence times. Mean particle size (MPS) between roughage sources (CS, SCB, and SC) and coproducts (SH and LOCH) was affected (P < 0.05). Dry matter intake (DMI) was not affected (P > 0.05) by 20CS, SC, SH, or LOCH. Steers fed 20CS or LOCH diets had 16% and 20% greater DMI, respectively, (P < 0.05) than steers fed 10CS diet. Steers fed SCB consumed the least dry matter (DM). The SH and LOCH diets had lower MPS values (about 8.77 mm) in comparison to 20CS, SCB, and SC diets (about 13.08 mm) and, consequently, affected (P < 0.05) rumen content, ruminal in situ disappearance, nutrient digestibility, and solid fractional passage rate. Chewing time was affected (P < 0.05) by roughage sources and concentration. Lower values of distance travel inside the rumen (min/cm) were observed (P < 0.05) for the SCB and SC diets in comparison with any other diet. Except for SCB, there was no difference (P > 0.05) in rumen fill, among other treatments. Mean daily ruminal pH was not affected (P > 0.05) by 20CS, SCB, SC, and LOCH diets, and it ranged from 6.1 to 6.23. Total short-chain fatty acids concentration was affected (P < 0.05) by roughage source and concentration. Based on our results, we recommend that under Brazilian finishing diets, replacing roughage sources, except for SCB, based on aNDF concentration of the roughage in high-concentrate diets containing finely ground flint corn does not affect DMI.

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Intake and performance of confined bovine fed fresh or ensilaged sugar cane based diets and corn silage

Revista Brasileira de Zootecnia ◽

10.1590/s1516-35982011000500022 ◽

2011 ◽

Vol 40 (5) ◽

pp. 1095-1103 ◽

Cited By ~ 4

Author(s):

Gustavo Chamon de Castro Menezes ◽

Sebastião de Campos Valadares Filho ◽

Felipe Antunes Magalhães ◽

Rilene Ferreira Diniz Valadares ◽

Laura Franco Prados ◽

...

Keyword(s):

Organic Matter ◽

Calcium Oxide ◽

Sugar Cane ◽

Dry Matter ◽

Subcutaneous Fat ◽

The Body ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Superior Performance ◽

And Performance

It was evaluated intake, total apparent digestibility, performance and feeding behavior of bovine fed diets constituted of corn silage, crushed sugar cane given fresh or crushed and given 72 hours after storage, ensilaged sugar cane with or without 1% of calcium oxide and concentrate at the proportion of 1% of the body weight. It was used 35 bovines, distributed in a random block design, with 5 treatments and 6 repetitions. The animals were housed in collective stalls with troughs individualized by electronic gates. The animals fed diet with corn silage presented greater intake of all nutrients and greater total digestibility of dry matter, organic matter, neutral detergent fiber and total digestible nutrients, as well as greater weight gain and subcutaneous fat thickness. Animals fed diet with fresh sugar cane presented greater nutrient intake as well as better dry matter digestibility, ether extract and TDN content and a superior performance in relation to animals fed ensilaged sugar cane diets. Animals fed silage of sugar cane with calcium oxide presented greater digestibility of organic matter, NFC and content of TDN but they did not differ on performance in relation to the use of silage of sugar cane without calcium oxide. Intake and performance of animals did not change with or without storage of sugar cane. It was concluded that animals fed diets with corn silage present performances superior to the ones which are fed sugar cane based diets, and animals fed fresh sugar diet are superior to the animals fed diets with sugar cane silage.

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Impact of corn silage moisture at harvest on performance of growing steers with supplemental rumen undegradable protein, finishing steer performance, and nutrient digestibility by lambs1

Translational Animal Science ◽

10.1093/tas/txz011 ◽

2019 ◽

Vol 3 (2) ◽

pp. 761-774 ◽

Cited By ~ 3

Author(s):

F Henry Hilscher ◽

Dirk B Burken ◽

Curt J Bittner ◽

Jana L Gramkow ◽

Robert G Bondurant ◽

...

Keyword(s):

Dry Matter ◽

Average Daily Gain ◽

Nutrient Digestibility ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Feed Ratio ◽

Finishing Cattle ◽

Distillers Grains With Solubles ◽

Finishing Diets ◽

Rumen Undegradable Protein

Abstract Three experiments evaluated delaying corn silage harvest, silage concentration, and source of supplemental protein on performance and nutrient digestibility in growing and finishing diets. Experiment 1 used 180 crossbred yearling steers (body weight [BW] = 428; SD = 39 kg) to evaluate corn silage dry matter (DM) (37% or 43%) and replacing corn with silage (15% or 45% of diet DM) in finishing diets containing 40% modified distillers grains with solubles. Experiment 2 used 60 crossbred steers (BW = 271; SD = 32 kg) to evaluate corn silage harvest DM (37% or 43%) and response to rumen undegradable protein (RUP) supplementation (0.5%, 1.4%, 2.4%, 3.3%, or 4.2% of diet DM) in silage growing diets. Experiment 3 used 9 crossbred lambs (BW = 30.1; SD = 4.1 kg) to evaluate nutrient digestibility of 37% or 43% DM corn silage in silage growing diets fed ad libitum or restricted to 1.5% of BW. In experiment 1, as corn silage concentration increased from 15% to 45%, average daily gain (ADG) and gain-to-feed ratio (G:F) decreased (P ≤ 0.04). Carcass-adjusted final BW and hot carcass weight (HCW) were lower (P ≤ 0.04) for steers fed 45% corn silage compared to 15% when fed for equal days. As DM of corn silage was increased from 37% to 43%, no differences (P ≥ 0.30) in dry matter intake (DMI), ADG, G:F, or HCW were observed. In experiment 2, as DM of corn silage increased from 37% to 43%, ADG and G:F decreased (P ≤ 0.04). Increasing supplemental RUP in the diet increased (P ≤ 0.05) ending BW, DMI, ADG, and G:F linearly as supplemental RUP increased from 0.5% to 4.2%. In experiment 3, there were no differences (P ≥ 0.56) in DM digestibility and organic matter digestibility between silage harvest DM and intake level. Neutral detergent fiber (NDF) intake was reduced (P < 0.01) for lambs fed the delayed harvest corn silage compared to earlier corn silage harvest. As silage harvest was delayed from 37% to 43% DM, NDF digestibility decreased (P < 0.01) from 64.39% to 53.41%. Although increasing corn silage concentration in place of corn in finishing diets reduced ADG and G:F, delayed silage harvest did not affect performance of finishing cattle. Delayed silage harvest in growing cattle resulted in lower ADG and G:F, possibly due to increased starch or maturity leading to decreased NDF digestibility. The addition of RUP to silage-based, growing diets improves performance by supplying more metabolizable protein and suggests RUP of corn silage is limiting.

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Corn Silage, Meta-Analysis of The Quality and Yield of Different Regions in the World

10.20944/preprints202010.0094.v1 ◽

2020 ◽

Author(s):

Isidro García-Chávez ◽

Edgar Meraz-Romero ◽

Octavio Castelán-Ortega ◽

Joob Zaragoza Esparza ◽

Jorge Osorio Avalos ◽

...

Keyword(s):

Milk Production ◽

Dry Matter ◽

Plant Density ◽

Sugar Content ◽

Soluble Sugar ◽

Energy Resource ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Energy Concentration ◽

The World

Corn silage (Zea mays L.) is the most widely used energy resource in the diets of dairy cattle around the world; it stands out for its higher biomass yields, good palatability, homogeneous quality at harvest and ease of silage due to its higher soluble sugar content. It was carried out a search of studies related to dry matter yield (ton ha-1), population density (plant density ha-1), dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), non-fibrous carbohydrates (NFC), organic matter (OM), DM digestibility (DMD) and neutral detergent fiber digestibility (NDFD) and milk production per hectare (kg of milk ha-1) that was determined using MILK2006®. It was carried out a cluster analysis (CL, PROC CLUSTER) obtaining six groups of corn silage: CL1: "Starch", which included DMD, DFDN, TDN1xDM, Mega calories per kg of DM and kg of milk/ton/DM; CL2: "Fats" which included TDN1xMS, Mcal/kg/DM and kg of milk/ton/DM; CL3: "Dry Matter", conformed only by DFDN; CL4: "Plant Density" including Yield of DM ha-1 and Milk Production ha-1; CL5: "Neutral Detergent Fiber" and CL6: "Raw Protein". It is concluded that CL1 was characterized by a higher DMD, DFND, CNF and starch that allow a higher TNDx1DM and an energy concentration (Mcal/kg/DM) that shows a higher milk production (kg of milk/ton/DM ha-1). The characteristics of CL2 with higher EE, allow a higher TNDx1DM and an energy concentration (Mcal/kg/DM) with a higher milk production (kg of milk/ton/DM ha-1).

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Use of barley silage or corn silage with dry-rolled barley, corn, or a blend of barley and corn on predicted nutrient total tract digestibility and growth performance of backgrounding steers

Canadian Journal of Animal Science ◽

10.1139/cjas-2019-0198 ◽

2020 ◽

pp. 1-9

Author(s):

B.D. Sutherland ◽

J.A. Johnson ◽

J.J. McKinnon ◽

T.A. McAllister ◽

G.B. Penner

Keyword(s):

Growth Performance ◽

Dry Matter ◽

Near Infrared ◽

Average Daily Gain ◽

Nutrient Digestibility ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Feed Ratio ◽

Gross Energy ◽

Barley Silage

The objective of this study was to determine the effects of feeding barley silage (BS) or corn silage (CS) with dry-rolled barley (BG), dry-rolled corn (CG), or a blend of barley and corn grain (BCG), on growth performance and nutrient digestibility, the latter predicted using near-infrared spectroscopy for backgrounding cattle. Steers (n = 288) were stratified by body weight (BW) into 24 pens and pens were randomly assigned to one of six treatments (n = 4) in a 2 × 3 factorial design. Main factors included BS or CS in combination with BG, CG, or BCG. There were no silage × grain interactions. Dry matter intake (DMI; P = 0.018) and final BW (P = 0.004) were greater for steers fed CS than BS, but average daily gain (1.01 kg d−1) and gain-to-feed ratio (0.10 kg kg−1) were not affected by silage or grain source. Steers fed CS also had greater dry matter, organic matter, crude protein (CP), acid detergent fiber (ADF), starch, and gross energy digestibility values (P < 0.01) than BS. Feeding BG increased starch, neutral detergent fiber, ADF, and CP digestibility values (P ≤ 0.01) over CG and BCG. Relative to BS, feeding CS increased DMI, final BW, and nutrient digestibility, whereas dry-rolled BG improved nutrient digestibility when compared with CG and BCG in backgrounding diets.

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Effects of Black Wattle (Acacia mearnsii) Condensed Tannins on Intake, Protozoa Population, Ruminal Fermentation, and Nutrient Digestibility in Jersey Steers

Animals ◽

10.3390/ani10061011 ◽

2020 ◽

Vol 10 (6) ◽

pp. 1011 ◽

Cited By ~ 4

Author(s):

Andre S. Avila ◽

Maximiliane A. Zambom ◽

Andressa Faccenda ◽

Maria L. Fischer ◽

Fernando A. Anschau ◽

...

Keyword(s):

Organic Matter ◽

Condensed Tannins ◽

Crude Protein ◽

Dry Matter ◽

Nutrient Utilization ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Ruminal Fermentation ◽

Acacia Mearnsii ◽

Black Wattle

The objective of this study was to evaluate the effect of inclusion of condensed tannins (CT) from black wattle (Acacia mearnsii) on feed intake, ruminal protozoa population, ruminal fermentation, and nutrient digestibility in Jersey steers. Five ruminally-cannulated steers were used in a 5 × 5 Latin square design, with five periods of 20 days each (14 days for diet adaptation and six days for sample collection per period). Treatments were composed of dietary inclusion levels of condensed tannins at 0, 5, 10, 15, and 20 g/kg of diet dry matter. Intakes of dry matter, organic matter, ether extract, crude protein, neutral detergent fiber, and total digestible nutrients were not affected by condensed tannins. The ruminal pH was reduced linearly with tannin levels. Ruminal ammonia nitrogen concentration was not affected by tannins. Tannins reduced the molar proportion of acetate and did not affect the ruminal protozoal population, which might be related to the low doses used. Digestibilities of dry matter, organic matter, and neutral detergent fiber were not altered; however, there was a linear reduction in crude protein digestibility. Based on these results, CT extracts from black wattle are not recommended for improving nutrient utilization in steers at the tested levels.

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Evaluation of rehydrated corn grain silage with okara or soybean inclusion in the performance of lactating cows

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n6p2747 ◽

2020 ◽

Vol 41 (6) ◽

pp. 2747-2758

Author(s):

Tamara Tais Tres ◽

◽

Clóves Cabreira Jobim ◽

Robson Marcelo Rossi ◽

Antonio Vinicius Iank Bueno ◽

...

Keyword(s):

Milk Production ◽

Feed Efficiency ◽

Dry Matter ◽

Latin Square ◽

Nutrient Digestibility ◽

Corn Silage ◽

Dry Matter Intake ◽

Lactating Cows ◽

Corn Grain ◽

Corn Grains

This study aimed to evaluate dry matter intake, nutrient digestibility, milk production and composition, and feed efficiency of cows fed corn grain silages with okara or raw soybean inclusion. Six Holstein cows were distributed in a double Latin square and arranged in the following treatments: concentrate formulated based on corn grain silage with 30% okara inclusion; concentrate of corn grain silage with 20% raw soybean inclusion, and concentrate based on dry corn grains. Statistical analysis was performed using Bayesian inference. Diets formulated with silages reduced dry matter intake compared to the diet of concentrate based on dry grains. High digestibility of DM, OM, NFC, and EE was observed for diets with silage inclusion. Milk presented an increase in CLA for the diet of corn grain silage diet with soybean inclusion. Rehydrated grain corn silage with soybean or okara inclusion reduced dry matter intake and increased nutrient digestibility, maintaining the same milk production, which demonstrates the higher efficiency of these diets.

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Evaluation of a high forage total mixed ration on the lactational performance of late lactation dairy cows

Translational Animal Science ◽

10.2527/tas2016.0011 ◽

2017 ◽

Vol 1 (1) ◽

pp. 108-115 ◽

Cited By ~ 1

Author(s):

D. L. Gadeken ◽

D. P. Casper

Keyword(s):

Data Collection ◽

Dairy Cows ◽

Milk Production ◽

Milk Fat ◽

Block Design ◽

Nutrient Digestibility ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Nutrient Requirements ◽

High Forage

Abstract Dairy producers continuously ask questions challenging the paradigm of how much forage can be included in the ration to meet the nutrient requirements of lactating dairy cows to support milk production. The production and feeding of forages having both high dry matter digestibility (DMD) and neutral detergent fiber digestibility (NDFd) are needed to increase nutrient supply. Mid- to late-lactation lactating Holstein dairy cows were blocked by parity (10 primiparous and 10 multiparous), milk production (range 33.9 to 56.6; μ = 41.5 kg/d), and days in milk (DIM) (range 140 to 287; μ = 225 d) and randomly assigned within blocks to 1 of 2 rations based on medium forage (MF) or high forage (HF) inclusion rates. A forage blend consisting of 60% second cutting (2012) alfalfa haylage and 40% (2012) corn silage blended on a DM basis and then fed at either 60% (MF) or 80% (HF) of the ration DM. The alfalfa haylage DM (DMD = 75.7%) and NDF (NDFd = 55.7%) digestibility was above average, but corn silage (DMD = 72.9, NDFd = 52.3%, and starch = 32.1%) was average. The experimental design was a randomized completed block design with 4 continuous weeks for data collection preceded by a 1 wk covariate data collection period in which all cows were fed the MF ration. Cows were milked 3 times/d and milk weights recorded at each milking and milk samples were collected at each milking once weekly for analysis of milk composition. Rations were similar in crude protein (CP; 16.4%), starch (20.1%), acid detergent fiber (ADF; 21.8%), and NDF (34.1%) concentrations. Covariately adjusted milk production (28.1 and 24.1 kg/d for MF and HF, respectively) and 4% fat-corrected milk (FCM; 27.6 and 24.1 kg/d) were significantly reduced by feeding the HF ration compared with cows fed the MF ration, while milk fat (3.98 and 4.0%), milk protein (3.11 and 3.17%), milk lactose (4.81 and 4.77%), and milk solids-not-fat (8.87 and 8.77%) percentages were similar for cows fed both rations. Cows on the HF ration demonstrated a significant reduction in DMI and a trend for decreased body weight (BW) when compared with cows fed the MF ration. The forage nutrient digestibility was not adequate to support the milk production of mid- to late-lactation dairy cows when fed at 80% of the DM. The forage nutrient digestibility when fed at very high inclusion rate (80%) could not meet the nutrient requirements of mid- to late-lactation dairy cows.

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