Treatment of Residual Periodontal Pockets Using a Hyaluronic Acid-Based Gel: A 12 Month Multicenter Randomized Triple-Blinded Clinical Trial

The aim of the present study was to evaluate the adjunctive effect of hyaluronic acid (HA) gel in the treatment of residual periodontal pockets over a 12-month period. Periodontal patients presenting at least one residual periodontal pocket 5–9 mm of depth in the anterior area were recruited from six university-based centers. Each patient was randomly assigned to subgingival instrumentation (SI) with the local adjunctive use of HA for test treatment or adjunctive use of local placebo for control treatment at baseline and after 3 months. Clinical parameters ( )probing depth (PD), bleeding on probing (BoP), plaque index (PI), recession (REC), clinical attachment level (CAL)) and microbiological samples for the investigation of the total bacterial count (TBC) and presence of specific bacterial species (Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum) were taken at baseline and every 3 months, until study termination. PD was determined as the primary outcome variable. From a total of 144 enrolled, 126 participants (53 males, 73 females) completed the entire protocol. Both treatments resulted in statistically significant clinical and microbiological improvements compared to baseline. Although the local application of HA showed a tendency for better results, there was a lack of statistically significant differences between the groups.

Download Full-text

Adjunctive air-polishing with erythritol in nonsurgical periodontal therapy: a randomized clinical trial

BMC Oral Health ◽

10.1186/s12903-020-01363-5 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Holger F. R. Jentsch ◽

Christian Flechsig ◽

Benjamin Kette ◽

Sigrun Eick

Keyword(s):

Test Group ◽

Nonparametric Tests ◽

Periodontal Therapy ◽

Periodontal Pocket ◽

Control Group ◽

Clinical Variables ◽

Air Polishing ◽

Probing Depth ◽

Nonsurgical Periodontal Therapy ◽

Attachment Level

Abstract Background This study was aimed to investigate if the adjunctive use of erythritol air-polishing powder applied with the nozzle-system during subgingival instrumentation (SI) has an effect on the outcome of non-surgical periodontal treatment in patients with moderate to severe periodontitis. Methods Fourty-two individuals with periodontitis received nonsurgical periodontal therapy by SI without (controls, n = 21) and with adjunctive air-polishing using nozzle + erythritol powder (test, n = 21). They were analyzed for the clinical variables BOP (primary outcome at six months), probing depth (PD), attachment level, four selected microorganisms and two biomarkers at baseline, before SI as well as three and six months after SI. Statistical analysis included nonparametric tests for intra- and intergroup comparisons. Results In both groups, the clinical variables PD, attachment level and BOP significantly improved three and six months after SI. The number of sites with PD ≥ 5 mm was significantly lower in the test group than in the control group after six months. At six months versus baseline, there were significant reductions of Tannerella forsythia and Treponema denticola counts as well as lower levels of MMP-8 in the test group. Conclusions Subgingival instrumentation with adjunctive erythritol air-polishing powder does not reduce BOP. But it may add beneficial effects like reducing the probing depth measured as number of residual periodontal pocket with PD ≥ 5 mm when compared with subgingival instrumentation only. Clinical relevance The adjunctive use of erythritol air-polishing powder applied with the nozzle-system during SI may improve the clinical outcome of SI and may reduce the need for periodontal surgery. Trial registration The study was retrospectively registered in the German register of clinical trials, DRKS00015239 on 6th August 2018, https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL.

Download Full-text

Treatment of Intrabony Defects with a Combination of Hyaluronic Acid and Deproteinized Porcine Bone Mineral

Materials ◽

10.3390/ma14226795 ◽

2021 ◽

Vol 14 (22) ◽

pp. 6795

Author(s):

Darko Božić ◽

Ivan Ćatović ◽

Ana Badovinac ◽

Larisa Musić ◽

Matej Par ◽

...

Keyword(s):

Hyaluronic Acid ◽

Bone Mineral ◽

Gingival Recession ◽

Intrabony Defects ◽

Clinical Attachment Level ◽

New Approach ◽

Novel Approach ◽

Probing Depth ◽

Baseline Values ◽

Attachment Level

Background: this study evaluates the clinical outcomes of a novel approach in treating deep intrabony defects utilizing papilla preservation techniques with a combination of hyaluronic acid (HA) and deproteinized porcine bone mineral. Methods: 23 patients with 27 intrabony defects were treated with a combination of HA and deproteinized porcine bone mineral. Clinical attachment level (CAL), pocket probing depth (PPD), gingival recession (REC) were recorded at baseline and 6 months after the surgery. Results: At 6 months, there was a significant CAL gain of 3.65 ± 1.67 mm (p < 0.001) with a PPD reduction of 4.54 ± 1.65 mm (p < 0.001), which was associated with an increase in gingival recession (0.89 ± 0.59 mm, p < 0.001). The percentage of pocket resolution based on a PPD ≤4 mm was 92.6% and the failure rate based on a PPD of 5 mm was 7.4%. Conclusions: the present findings indicate that applying a combined HA and xenograft approach in deep intrabony defects provides clinically relevant CAL gains and PPD reductions compared to baseline values and is a valid new approach in treating intrabony defects.

Download Full-text

Effect of Feed Supplemented With Vitamin C on Microbial Flora of Swine Faeces and Blood Morphology

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/v10213-012-0031-3 ◽

2012 ◽

Vol 56 (2) ◽

pp. 171-175

Author(s):

Beata Trawińska ◽

Jerzy Lechowski ◽

Antoni Polonis ◽

Marta Kowaleczko

Keyword(s):

Vitamin C ◽

Pathogenic Bacteria ◽

Bacterial Species ◽

Intestinal Flora ◽

Bacterial Count ◽

Control Group ◽

Total Bacterial Count ◽

E Coli ◽

Bacteriological Evaluation ◽

Experimental Group

Abstract The studies involved gilts 6 weeks pre-partum allocated into the control and experimental groups. The experimental group received vitamin C at the dose of 2.5g/100 kg b.w./day. Faeces for analyses were collected for 3- and 6- week period of vitamin C dietary inclusion. The faecal material underwent quantitative and qualitative bacteriological evaluation. The count of Enterobacteriaceae was evaluated. The qualitative examination of these bacteria was made using the micro-plate method API 20E. Feed supplemented with vitamin C contributed to a statistically significant decline of the total bacterial count of Enterobacteriaceae in faeces. The qualitative bacterial analyses showed the presence of E. coli, Providecia sp., Proteus sp., and Salmonella sp. in the control and experimental groups. Enterobacter sp. was recovered only in the control group, while Lactobacillus sp. in the experimental group. The dietary administration of vitamin C significantly decreased the number of the studied bacterial species, except Salmonella rods. No statistically significant differences in the number of most blood morphotic elements following the 6- week supply of vitamin C were found; only the granulocyte count significantly increased, while lymphocyte numbers declined. Ascorbic acid inhibited the growth of pathogenic intestinal flora and reduced the pathogenic and relatively pathogenic bacteria count in the gastrointestinal tract and notably contributed to enhanced growth of beneficial bacteria.

Download Full-text

Adjunctive air-polishing with erythritol in nonsurgical periodontal therapy – a randomized clinical trial

10.21203/rs.3.rs-47863/v2 ◽

2020 ◽

Author(s):

Holger F.R. Jentsch ◽

Christian Flechsig ◽

Benjamin Kette ◽

Sigrun Eick

Keyword(s):

Test Group ◽

Nonparametric Tests ◽

Periodontal Therapy ◽

Periodontal Pocket ◽

Control Group ◽

Clinical Variables ◽

Air Polishing ◽

Probing Depth ◽

Nonsurgical Periodontal Therapy ◽

Attachment Level

Abstract Background: This study was aimed to investigate if the adjunctive use of erythritol air-polishing powder applied with the nozzle-system during subgingival instrumentation (SI) has an effect on the outcome of non-surgical periodontal treatment in patients with moderate to severe periodontitis. Methods:Forty-two individuals with periodontitis received nonsurgical periodontal therapy by SI without (controls, n=21) and with adjunctive air-polishing using nozzle + erythritol powder (test, n=21). They were analyzed for the clinical variables BOP (primary outcome at six month), probing depth (PD), attachment level (AL), four selected microorganisms and two biomarkers at baseline, before SI as well as three and six months after SI. Statistical analysis included nonparametric tests for intra- and intergroup comparisons. Results: In both groups, the clinical variables probing depth (PD), attachment level and BOP significantly improved three and six months after SI. The number of sites with PD ≥ 5mm was significantly lower in the test group than in the control group after six months. At six months vs. baseline, there were significant reductions of Tannerella forsythia and Treponema denticola counts as well as lower levels of MMP-8 in the test group. Conclusions:Subgingival instrumentation with adjunctive erythritol air-polishing powder does not reduce BOP. But it may add beneficial effects like reducing the probing depth measured as number of residual periodontal pocket with PD ≥ 5mm when compared with subgingival instrumentation only. Clinical relevanceThe adjunctive use of erythritol air-polishing powder applied with the nozzle-system during SI may improve the clinical outcome of SI and may reduce the need for periodontal surgery.

Download Full-text

Relationship between Oral Malodor and the Global Composition of Indigenous Bacterial Populations in Saliva

Applied and Environmental Microbiology ◽

10.1128/aem.02304-09 ◽

2010 ◽

Vol 76 (9) ◽

pp. 2806-2814 ◽

Cited By ~ 38

Author(s):

Toru Takeshita ◽

Nao Suzuki ◽

Yoshio Nakano ◽

Yoshihiro Shimazaki ◽

Masahiro Yoneda ◽

...

Keyword(s):

Microbial Community Composition ◽

Hierarchical Cluster ◽

Bacterial Count ◽

The Other ◽

Periodontal Pocket ◽

Total Bacterial Count ◽

Oral Malodor ◽

Pocket Depth ◽

Bacterial Populations ◽

Two Parameters

ABSTRACT Oral malodor develops mostly from the metabolic activities of indigenous bacterial populations within the oral cavity, but whether healthy or oral malodor-related patterns of the global bacterial composition exist remains unclear. In this study, the bacterial compositions in the saliva of 240 subjects complaining of oral malodor were divided into groups based on terminal-restriction fragment length polymorphism (T-RFLP) profiles using hierarchical cluster analysis, and the patterns of the microbial community composition of those exhibiting higher and lower malodor were explored. Four types of bacterial community compositions were detected (clusters I, II, III, and IV). Two parameters for measuring oral malodor intensity (the concentration of volatile sulfur compounds in mouth air and the organoleptic score) were noticeably lower in cluster I than in the other clusters. Using multivariate analysis, the differences in the levels of oral malodor were significant after adjustment for potential confounding factors such as total bacterial count, mean periodontal pocket depth, and tongue coating score (P < 0.001). Among the four clusters with different proportions of indigenous members, the T-RFLP profiles of cluster I were implicated as the bacterial populations with higher proportions of Streptococcus, Granulicatella, Rothia, and Treponema species than those of the other clusters. These results clearly correlate the global composition of indigenous bacterial populations with the severity of oral malodor.

Download Full-text

Identification and antibiotic susceptibility of bacterial microbiota of freshwater fish

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1063 ◽

2019 ◽

Vol 13 (1) ◽

pp. 408-414

Author(s):

Alina Klūga ◽

Miroslava Kačániová ◽

Margarita Terentjeva

Keyword(s):

Antibiotic Susceptibility ◽

Negative Impact ◽

Bacterial Species ◽

Bacterial Count ◽

Diffusion Method ◽

Resistant Bacteria ◽

Total Bacterial Count ◽

Pseudomonas Spp ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Fish Samples

The fish meat is an essential part of human diet. However, fish may be contaminated with different microorganisms, including pathogens. Antimicrobial resistance of fish microbiota may facilitate the spread of resistant microorganisms causing serious consequences for human health. The aim of the present study was to detect bacterial contamination in fish gill, gut and skin and to determine antimicrobial susceptibility of the bacterial isolates. Rainbow trout (Oncorhynchus mykiss) and bream (Abramis bram) were obtained from the market in Jelgava city. Chub (Leuciscus cephalus), crucian carp (Carassius carassius) and tench (Tinca tinca) were collected from fishermen. Fish samples were examined for the total bacterial count (TBC), coliforms, Enterobacteriaceae, Pseudomonas spp. and Aeromonas spp. Testing was done in accordance with International Organization for Standardization (ISO) standards. Identification of all bacteria was accomplished with the Matrix Assisted Laser Desorption Ionization – Time of Flight Mass Spectrometry (MALDI-TOF MS) method. The disc diffusion method was used for the detection of antibiotic susceptibility of isolated bacterial species. TBC ranged from 2.70 to 7.00 log CFU.g-1, coliforms from 0 to 2.67 log CFU.g-1, Enterobacteriaceae from 0 to 2.85 log CFU.g-1. The highest contamination with Pseudomonas spp. and Aeromonas spp. was observed in chub gut samples with 1.60 log CFU.g-1 and 2.23 log CFU.g-1, respectively. Altogether, 16 microbial genera and 31 bacterial species were identified. The dominant bacterial species belonged to Pseudomonas spp. (54%) and Enterobacteriaceae. Pseudomonas spp. were resistant to ticarcillin, susceptibility to ciprofloxacin showed 88% of isolates. All Enterobacteriaceae isolates were susceptible to imipenem. The microbial quality of the fish was acceptable, but the presence of antibiotic resistant bacteria may further cause a negative impact on public health.

Download Full-text

Anti-Early Stage of Bacterial Recolonization Effect of Curcuma longa Extract as Photodynamic Adjunctive Treatment

International Journal of Dentistry ◽

10.1155/2020/8823708 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Doosadee Hormdee ◽

Weena Rinsathorn ◽

Subin Puasiri ◽

Paiboon Jitprasertwong

Keyword(s):

Early Stage ◽

Curcuma Longa ◽

Fusobacterium Nucleatum ◽

Periodontal Pocket ◽

Adjunctive Treatment ◽

Photodynamic Treatment ◽

Posterior Teeth ◽

Light Emitting ◽

Probing Depth ◽

Bacterial Recolonization

Objective. To evaluate the amount of Fusobacterium nucleatum (F. nucleatum) and Prevotella intermedia (P. intermedia) on subgingival recolonized plaque after mechanical debridement and photodynamic treatment by using blue light-emitting diodes (LEDs) in combination with topical Curcuma longa gel extract. Methods. A total of 12 subjects with stage III grade B periodontitis were recruited for the study. Maxillary posterior teeth with periodontal pocket >4 mm were selected. These teeth were examined for periodontal clinical data at baseline and at 1, 2, 4, and 6 weeks after treatment. All remaining teeth were treated by scaling and root planing (SRP). Then, the teeth were bilaterally divided using randomized split-mouth design with and without photodynamic adjunctive therapy (PDT). Samples of the subgingival microbiota were obtained in each visit. All samples were analyzed by multicolor TaqMan real-time polymerase chain reaction (PCR) for the presence of target bacteria. Results. Throughout the six-week follow-up, long-term improvement of probing depth and bleeding on probing was revealed on the PDT group. The number of subgingival F. nucleatum and P. intermedia also significantly reduced, compared to the baseline. There was a statistically significant recolonization in F. nucleatum and P. intermedia number after 2 and 4 weeks of conventional SRP, respectively. Our quantitative PCR method showed no significant recolonization of those subgingival bacteria on PDT sites throughout the 6-week study duration. Conclusion. The results showed that adjunctive photodynamic treatment by using blue LEDs in combination with topical Curcuma longa gel extract was effective to alter the recolonization patterns of F. nucleatum and P. intermedia after conventional debridement.

Download Full-text

Adjunctive air-polishing with erythritol in nonsurgical periodontal therapy – a randomized clinical trial

10.21203/rs.3.rs-47863/v3 ◽

2020 ◽

Author(s):

Holger F.R. Jentsch ◽

Christian Flechsig ◽

Benjamin Kette ◽

Sigrun Eick

Keyword(s):

Test Group ◽

Nonparametric Tests ◽

Periodontal Therapy ◽

Periodontal Pocket ◽

Control Group ◽

Clinical Variables ◽

Air Polishing ◽

Probing Depth ◽

Nonsurgical Periodontal Therapy ◽

Attachment Level

Abstract Background: This study was aimed to investigate if the adjunctive use of erythritol air-polishing powder applied with the nozzle-system during subgingival instrumentation (SI) has an effect on the outcome of non-surgical periodontal treatment in patients with moderate to severe periodontitis. Methods: Fourty-two individuals with periodontitis received nonsurgical periodontal therapy by SI without (controls, n=21) and with adjunctive air-polishing using nozzle + erythritol powder (test, n=21). They were analyzed for the clinical variables BOP (primary outcome at six month), probing depth (PD), attachment level (AL), four selected microorganisms and two biomarkers at baseline, before SI as well as three and six months after SI. Statistical analysis included nonparametric tests for intra- and intergroup comparisons. Results: In both groups, the clinical variables probing depth (PD), attachment level and BOP significantly improved three and six months after SI. The number of sites with PD ≥ 5mm was significantly lower in the test group than in the control group after six months. At six months vs. baseline, there were significant reductions of Tannerella forsythia and Treponema denticola counts as well as lower levels of MMP-8 in the test group. Conclusions: Subgingival instrumentation with adjunctive erythritol air-polishing powder does not reduce BOP. But it may add beneficial effects like reducing the probing depth measured as number of residual periodontal pocket with PD ≥ 5mm when compared with subgingival instrumentation only. Clinical relevance: The adjunctive use of erythritol air-polishing powder applied with the nozzle-system during SI may improve the clinical outcome of SI and may reduce the need for periodontal surgery. Trial registration: The study was retrospectively registered in the German register of clinical trials, DRKS00015239 on 6th August 2018, https://www.drks.de/drks_web/navigate.do?navigationId =trial.HTML&TRIAL

Download Full-text

Determination of Microbial Activity and Quality Traits of Eggs Coated with Propolis

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v8i6.1380-1384.3385 ◽

2020 ◽

Vol 8 (6) ◽

pp. 1380-1384

Author(s):

Sezai Alkan ◽

Ömer Ertürk ◽

İsmail Türker

Keyword(s):

Microbial Activity ◽

Storage Time ◽

Egg Quality ◽

Bacterial Species ◽

Bacterial Count ◽

Total Bacterial Count ◽

Quality Traits ◽

Bacterial Counts ◽

Local Farm

Eggs were coated with propolis in order to determine its effect on egg quality and total bacterial count. Effect of storage time prior to propolis coating was also studied. Eggs were obtained from a local farm, coated with 0, 3, 6 or 9% of propolis and stored at 4 ºC with a humidity of 75% for 30 days. A total of 18 eggs were used for egg quality traits and 6 eggs were chosen for microbial activity for each group. Bacteria were recovered with sterile cotton swabs from egg surfaces. Colonies with different morphology and color were detected. 11 bacterial species were determined following the characterization of 14 isolates. Elevated levels of bacterial counts were detected for daily eggs, which were significantly higher than those stored for 30 days. The quality traits of the daily eggs have also been found higher compared with the other groups. It was observed that coating the eggs with varying concentrations of propolis lead to significant differences in the total bacterial counts of the eggshells. Moreover, the egg quality traits which decreased with the increase in storage time have not been further affected with propolis coating.

Download Full-text

Bacteriological assessment of some borehole water samples in Mile 50, Abakaliki, Ebonyi State, Nigeria

Archives of Agriculture and Environmental Science ◽

10.26832/24566632.2020.0502015 ◽

2020 ◽

Vol 5 (2) ◽

pp. 179-189

Author(s):

Eziafakaego M. Ibo ◽

Odera R. Umeh ◽

Bright O. Uba ◽

Pius I. Egwuatu

Keyword(s):

Water Samples ◽

Bacterial Species ◽

Bacterial Count ◽

Salmonella Typhi ◽

Dry Season ◽

Total Bacterial Count ◽

Dilution Method ◽

Peptone Water ◽

Borehole Water ◽

Level Of Significance

Water as excellent natural resource is meant to be of high quality to reduce the outbreak of water-borne diseases. Bacteriological load of some borehole water samples in Mile 50 Abakaliki were carried out to determine their potability. Fifteen borehole water samples were sampled during rainy and dry season from June to July and November to December 2018 respectively. The total bacterial count was determined by tenfold serial dilution method using peptone water. Eight bacterial species namely Vibrio cholerae, Pseudomonas aeroginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Shigella flexineri, Proteus vulgaris and Klebsiella pneumoniaewere isolated using standard analytical procedures. The bacterium that had the highest frequency of occurrence during both rainy and dry season’s was K. pneumoniae with percentage frequency of 21.81% and 20.79% respectively, and P. vulgaris had the least value of 6.96% during rainy season. E. coli and S. aureus have the least value of 5.94% during dry season. Amoxicillin (30ug) was mostly resisted by the bacterial isolates why being was more susceptible to Ciprofloxacin (10ug) among the antibiotics used for susceptibility test. Two way analysis of variance (ANOVA) was used to determine the level of significance among the bacteriological analyses of both seasons. Therefore, there is need to create awareness about the present situation of the borehole waters and the necessity for further treatment of water by consumers, before it can be used for both drinking and domestic purposes to prevent disease outbreak in the area.

Download Full-text