scholarly journals Adding Two Antimicrobial Glasses to an Endodontic Sealer to Prevent Bacterial Root Canal Reinfection: An In Vivo Pilot Study in Dogs

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1183
Author(s):  
Álvaro Zubizarreta-Macho ◽  
Cristina Rico-Romano ◽  
María Jesús Fernández-Aceñero ◽  
Jesús Mena-Álvarez ◽  
Belén Cabal ◽  
...  
Keyword(s):  
Glass Ceramic ◽  
Root Canal ◽  
Tissue Response ◽  
Control Group ◽  
Root Canal Sealer ◽  
Bacterial Reduction ◽  
Canal Systems ◽  
Histological Results

Current endodontic procedures continue to be unsuccessful for completely removing pathogens present inside the root canal system, which can lead to recurrent infections. In this study, we aimed to assess the antimicrobial capacity and tissue response of two inorganic bactericidal additives incorporated into a paste root canal sealer on contaminated root dentin in vivo. An experimental study was performed in 30 teeth of five Beagle dogs. After inducing microbiological contamination, root canal systems were treated by randomly incorporating one of two antimicrobial additives into a commercial epoxy-amine resin sealer (AH Plus), i.e., G3T glass-ceramic (n = 10) and ZnO-enriched glass (n = 10); 10 samples were randomized as a control group. After having sacrificed the animals, microbiological, radiological, and histological analyses were performed, which were complemented with an in vitro bactericidal test and characterization by field emission scanning electron microscopy. The tested groups demonstrated a non-significant microbiological reduction in the postmortem periapical index values between the control group and the bactericidal glass-ceramic group (p = 0.885), and between the control group and the ZnO-enriched glass group (p = 0.169). The histological results showed low values of inflammatory infiltrate, and a healing pattern characterized by fibrosis in 44.4% of the G3T glass-ceramic and 60.0% of ZnO-enriched glass. Bactericidal glassy additives incorporated in this root canal sealer are safe and effective in bacterial reduction.

scholarly journals Physical properties and biocompatibility of an injectable calcium-silicate-based root canal sealer: in vitro and in vivo study

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Eun-Su Lim ◽  
Young-Bae Park ◽  
Young-Sun Kwon ◽  
Won-Jun Shon ◽  
Kwang-Won Lee ◽  
...  
Keyword(s):  
Physical Properties ◽  
Root Canal ◽  
Calcium Silicate ◽  
In Vivo Study ◽  
Root Canal Sealer

scholarly journals The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response

2019 ◽  
Vol 20 (8) ◽  
pp. 1969 ◽  
Author(s):  
Dominik Sieger ◽  
Tadas Korzinskas ◽  
Ole Jung ◽  
Sanja Stojanovic ◽  
Sabine Wenisch ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bone Substitute ◽  
Tissue Response ◽  
Control Group ◽  
L929 Cells ◽  
High Doses ◽  
Tissue Reactions ◽  
Inflammatory Macrophages

Biphasic bone substitutes (BBS) are currently well-established biomaterials. Through their constant development, even natural components like hyaluronic acid (HY) have been added to improve both their handling and also their regenerative properties. However, little knowledge exists regarding the consequences of the addition of HY to their biocompatibility and the inflammatory tissue reactions. Thus, the present study was conducted, aiming to analyze the influence of two different amounts of high molecular weight HY (HMWHY), combined with a BBS, on in vitro biocompatibility and in vivo tissue reaction. Established in vitro procedures, using L929 cells, were used for cytocompatibility analyses under the test conditions of DIN EN:ISO 10993-5. For the in vivo part of the study, calvarial defects were created in 20 Wistar rats and subsequently filled with BBS, and BBS combined with two different HMWHY amounts, i.e., BBS + HY(L) and BBS + HY(H). As controls, empty defects were used. Established histological, immunohistochemical, and histomorphometrical methods were applied to analyze the tissue reactions to the three different materials, including the induction of pro- and anti-inflammatory macrophages and multinucleated giant cells (BMGCs). The in vitro results showed that none of the materials or compositions caused biological damage to the L929 cells and can be considered to be non-toxic. The in vivo results showed that only the addition of high doses of HY to a biphasic bone substitute significantly decreases the occurrence of pro-inflammatory macrophages (* p < 0.05), comparable to the numbers found in the control group, while no significant differences within the three study groups for M2-macrophages nor BMGCs were detected. In conclusion, the addition of different amounts of HMWHY does not seem to affect the inflammation response to BBS, while improving the material handling properties.

scholarly journals Debris Removal by Activation of Endodontic Irrigants in Complex Root Canal Systems: A Standardized In-Vitro-Study

2021 ◽  
Vol 11 (16) ◽  
pp. 7331
Author(s):  
Matthias Widbiller ◽  
Lukas Keim ◽  
Ralf Schlichting ◽  
Birgit Striegl ◽  
Karl-Anton Hiller ◽  
...  
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
In Vitro Study ◽  
Model System ◽  
Control Group ◽  
Complex Root ◽  
Micro Computed Tomography ◽  
Canal Systems ◽  
Endodontic Irrigation

Aim of the study was to develop a standardized model system to investigate endodontic irrigation techniques and assess the efficiency of different activation methods on the removal of hard tissue debris in complex root canal systems. Mesial roots of mandibular molars were firstly scanned by micro-computed tomography (µCT) and allocated to three groups of irrigant activation: sonic activation (EDDY, VDW, Munich, Germany), laser activation (AutoSWEEPS, FOTONA, Ljubljana, Slovenia) and conventional needle irrigation (control). Roots were fixed in individual 3D-printed holders to facilitate root canal enlargement under constant irrigation with NaOCl (5%). To enable standardized quantification of remaining debris, BaSO4-enriched dentine powder was compacted into the canals, followed by another µCT-scan. The final irrigation was performed using 17% ethylenediaminetetraacetic acid (EDTA) and 5% sodium hypochlorite (NaOCl) with the respective activation method, and the volume of remaining artificial debris was quantified after a final µCT-scan. The newly developed model system allowed for reliable, reproducible and standardized assessment of irrigation methods. Activation of the irrigant proved to be significantly more effective than conventional needle irrigation regarding the removal of debris, which persisted particularly in the apical third of the root canal in the control group. The efficiency of irrigation was significantly enhanced with laser- and sonic-based activation, especially in the apical third.

scholarly journals Photonic real-time monitoring of bacterial reduction in root canals by genetically engineered bacteria after chemomechanical endodontic therapy

2007 ◽  
Vol 18 (3) ◽  
pp. 202-207 ◽  
Author(s):  
Aguinaldo Silva Garcez ◽  
Silvia Cristina Nunez ◽  
José Luis Lage-Marques ◽  
Michael R. Hamblin ◽  
Martha Simões Ribeiro
Keyword(s):  
Real Time ◽  
Root Canal ◽  
Imaging System ◽  
Genetically Engineered ◽  
Microbial Infection ◽  
Bacterial Reduction ◽  
Culture Methods ◽  
Root Canals

Microbial infection plays an important role in the development of pulp necrosis and formation of periapical lesions. In vitro and in vivo research in this field, traditionally microbiological culture methods using paper point sampling and quantitative culture, faces difficulties in completely removing bacteria from the root canal system and analyzing sequential procedures. This study employed genetically engineered bioluminescent bacteria and a light-sensitive imaging system to allow real-time visualization of the infection. Ten extracted teeth incubated with P. aeruginosa were treated by mechanical instrumentation with K-files (#30 K-file, #35 K-file and #40 K-file) and chemical irrigation with sodium hypochlorite and hydrogen peroxide. Irrigation alone reduced the contamination in 18%; the first chemomechanical sequence (instrumentation with a #30 K-file + irrigation) provided 41% of reduction; the second sequence (#35 K-file + irrigation) achieved 62%; and the complete therapy (#30 K-file + #35 K-file + #40 K-file + irrigation) achieved 93% of bacterial reduction. These results suggest that the endodontic treatment is dependent on the association of a chemical and mechanical approaches and that root canal enlargement improves bacterial reduction probably because the irrigation has more access to the apical third.

scholarly journals Biocompatibility of Root Canal Sealers: A Systematic Review of In Vitro and In Vivo Studies

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4113 ◽  
Author(s):  
Diogo Afonso Fonseca ◽  
Anabela Baptista Paula ◽  
Carlos Miguel Marto ◽  
Ana Coelho ◽  
Siri Paulo ◽  
...  
Keyword(s):  
Systematic Review ◽  
Root Canal ◽  
Biological Effects ◽  
Tissue Response ◽  
In Vivo Studies ◽  
Cochrane Library ◽  
Toxic Potential ◽  
Root Canal Sealers

(1) Aim: To perform a systematic review of the literature on the biocompatibility of root canal sealers that encompasses the various types of sealers that are commercially available as well as both in vitro and in vivo evidence. (2) Methods: This systematic review has been registered in PROSPERO (ID 140445) and was carried out according to PRISMA guidelines using the following databases: PubMed, Cochrane Library, ClinicalTrials.gov, Science Direct, and Web of Science Core Collection. Studies published between 2000 and 11 June 2019 that evaluated cytotoxicity (cell viability/proliferation) and biocompatibility (tissue response) of root canal sealers were included. (3) Results: From a total of 1249 studies, 73 in vitro and 21 in vivo studies were included. In general, studies suggest that root canal sealers elicit mild to severe toxic effects and that several factors may influence biocompatibility, e.g., material setting condition and time, material concentration, and type of exposure. Bioactive endodontic sealers seem to exhibit a lower toxic potential in vitro. (4) Conclusions: The available evidence shows that root canal sealers exhibit variable toxic potential at the cellular and tissue level. However, the methodological heterogeneity among studies included in this systematic review and the somewhat conflicting results do not allow a conclusion on which type of sealer presents higher biocompatibility. Further research is crucial to achieve a better understanding of the biological effects of root canal sealers.

scholarly journals Different Methods of Studying Root Canal Morphology of Human Tooth: A Review

2015 ◽  
Vol 5 (2) ◽  
pp. 59-63
Author(s):  
Piyali Datta ◽  
Shabnam Zahir ◽  
Gautam Kumar Kundu ◽  
Kaushik Dutta
Keyword(s):  
Root Canal ◽  
Human Tooth ◽  
Root Canal Therapy ◽  
Canal Systems ◽  
Root Canal Morphology ◽  
Research And Education ◽  
Metal Castings ◽  
Canal Morphology

Successful root canal therapy requires an in-depth knowledge of root canal morphology.False assumptions about the root canal anatomy of teeth may lead to misdiagnosis, missed canals, improper debridement and breakage of root canal instruments during root canal treatment. The objective of this paper was to review the various methods used to study and understand root canal systems. The complexity of root canal morphology presents a challenge to any clinician. Any attempt to perform root canal therapy must be preceded with a thorough understanding of the anatomy of both the pulp chamber and the root canal system.Several methods have been used to examine the root canal system ranging from in vitro methods such as sectioning of teeth, metal castings to advanced in vivo tomographic imaging, along with the use of magnificationBangladesh Journal of Dental Research and Education Vol.5(2) 2015: 59-63

scholarly journals A influência de diferentes cimentos de obturação de canal na infiltração apical

2021 ◽  
Vol 36 (1) ◽  
pp. 24-26
Author(s):  
Elaine Vianna Freitas Fachin ◽  
Marilia Nedel Sperb ◽  
Tereza Maria Prietsch Kohler
Keyword(s):  
Statistical Analysis ◽  
Root Canal ◽  
In Vitro Study ◽  
Control Group ◽  
Root Canal Sealer ◽  
Dye Penetration ◽  
Root Canal Sealers ◽  
Clearing Method ◽  
Lower Incisors

It is an "in vitro" study to evaluate the sealing ability of 3 root canal sealers using a dye leakage/clearing method. 40 lower incisors were instrumented by the step-back technique and divided into 4 groups. The teeth were filled with lateral condensation of guta-percha varying the root canal sealer. Fill Canal, Sealer 26, N-Rickert were tested and compared to the control group in which guttapercha cones with no seater were used. Linear dye penetration was measured after demineralizatÎon and clearing. Statistical analysÏs showed significant differences among the groups. The lowest leakage values were found in the Fill Canal group followed by the N-Rickert and the Sealer 26 groups.

In-vitro- und In-vivo-Wirkung von Schilddrüsenhormon auf das Wachstum von Neuroblastomzellen

1990 ◽  
Vol 29 (03) ◽  
pp. 120-124
Author(s):  
R. P. Baum ◽  
E. Rohrbach ◽  
G. Hör ◽  
B. Kornhuber ◽  
E. Busse
Keyword(s):  
Cell Differentiation ◽  
Neuroblastoma Cells ◽  
Control Group ◽  
Initial Value ◽  
Initial Rise ◽  
Biphasic Effect ◽  
Contrast Microscopy ◽  
Morphological Sign

The effect of triiodothyronine (T3) on the differentiation of cultured neuroblastoma (NB) cells was studied after 9 days of treatment with a dose of 10-4 M/106 cells per day. Using phase contrast microscopy, 30-50% of NB cells showed formation of neurites as a morphological sign of cellular differentiation. The initial rise of the mitosis rate was followed by a plateau. Changes in cyclic nucleotide content, in the triphosphates and in the activity of the enzyme ornithine decarboxylase (ODC) were assessed in 2 human and 2 murine cell lines to serve as biochemical parameters of the cell differentiation induced by T3. Whereas the cAMP level increased significantly (3 to 7 fold compared with its initial value), the cGMP value dropped to 30 to 50% of that of the control group. ATP and GTP increased about 200%, the ODC showed a decrease of about 50%. The present studies show a biphasic effect of T3 on neuroblastoma cells: the initial rise of mitotic activity is followed by increased cell differentiation starting from day 4 of the treatment.

Protective Effect of Boswellic Resin Against Memory Loss and Alzheimer’s Induced by Aluminum Tetrachloride and D-Galactose (Experimental study in Mice)

2020 ◽  
Author(s):  
K. Zerrouki ◽  
N. Djebli ◽  
L. Gadouche ◽  
I. Erdogan Orhan ◽  
F. SezerSenol Deniz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Protective Effect ◽  
Cell Damage ◽  
Memory Loss ◽  
Control Group ◽  
Total Extract ◽  
Swiss Mice ◽  
Octyl Acetate

Nowadays, because of the industrialization, a lot of contaminant were available ; the consequences of this availability are apparition of diseases including neurodegeneration. Neurodegenerative diseases of the human brain comprise a variety of disorders that affect an increasing percentage of the population. This study is based on the effect of the Boswellic resin, which is from a medicinal plant and known for its antioxidant effects on nerve cell damage. The objective of this work was to evaluate the in vitro and in vivo effects of the Boswellic resin on anticholinesterase activity and Alzheimer’s disease (AD) induced by D-galactose and aluminum tetrachloride in Swiss mice. Chemical composition of the resin essential oil was identified by the CG-MS analysis. The antioxidant activity was also assessed by the DMPD and metal chelation methods. In order to understand the mechanism of memory improvement, the acetylcholinesterase, AChE, and butyrylcholinesterase, BChE, inhibitory assays were performed. In vivo part of the study was achieved on Swiss mice divided into four groups: control, AD model, treated AD, and treated control group. The identification of chemical composition by CG-MS reach the 89.67% of the total extract compounds presented some very important molecules (p-Cymene, n-Octyl acetate, α-Pinene…). The present study proves that Boswellic resin improves memory and learning in treated Alzheimer’s group, modulates the oxidative stress and be involved in the protective effect against amyloid deposition and neurodegeneration, and stimulates the immune system in mice’s brain.

Cold Plasma Treatment Promotes Full-thickness Healing of Skin Wounds in Murine Models

2021 ◽  
pp. 153473462110021
Author(s):  
Joon M. Jung ◽  
Hae K. Yoon ◽  
Chang J. Jung ◽  
Soo Y. Jo ◽  
Sang G. Hwang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plasma Treatment ◽  
Cold Plasma ◽  
Smooth Muscle Actin ◽  
Treated Group ◽  
Control Group ◽  
Alpha Smooth Muscle Actin ◽  
Skin Wound Healing

Cold plasma can be beneficial for promoting skin wound healing and has a high potential of being effectively used in treating various wounds. Our aim was to verify the effect of cold plasma in accelerating wound healing and investigate its underlying mechanism in vitro and in vivo. For the in vivo experiments, 2 full-thickness dermal wounds were created in each mouse (n = 30). While one wound was exposed to 2 daily plasma treatments for 3 min, the other wound served as a control. The wounds were evaluated by imaging and histological analyses at 4, 7, and 11 days post the wound infliction process. Immunohistochemical studies were also performed at the same time points. In vitro proliferation and scratch assay using HaCaT keratinocytes and fibroblasts were performed. The expression levels of wound healing–related genes were analyzed by real-time polymerase chain reaction and western blot analysis. On day 7, the wound healing rates were 53.94% and 63.58% for the control group and the plasma-treated group, respectively. On day 11, these rates were 76.05% and 93.44% for the control and plasma-treated groups, respectively, and the difference between them was significant ( P = .039). Histological analysis demonstrated that plasma treatment promotes the formation of epidermal keratin and granular layers. Immunohistochemical studies also revealed that collagen 1, collagen 3, and alpha-smooth muscle actin appeared more abundantly in the plasma-treated group than in the control group. In vitro, the proliferation of keratinocytes was promoted by plasma exposure. Scratch assay showed that fibroblast exposure to plasma increased their migration. The expression levels of collagen 1, collagen 3, and alpha-smooth muscle actin were elevated upon plasma treatment. In conclusion, cold plasma can accelerate skin wound healing and is well tolerated.

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