scholarly journals Effects of Selenium and Cadmium on Breast Muscle Fatty-Acid Composition and Gene Expression of Liver Antioxidant Proteins in Broilers

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 147 ◽  
Author(s):  
Evangelos Zoidis ◽  
George Papadomichelakis ◽  
Athanasios C. Pappas ◽  
Georgios Theodorou ◽  
Kostas Fegeros
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Messenger Rna ◽  
Broiler Chickens ◽  
Thioredoxin Reductase ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Breast Muscle ◽  
Iodothyronine Deiodinase ◽  
Organic Selenium

The present work was part of a project intended to evaluate whether organic selenium (Se) has the potential to protect against toxic effects exerted by cadmium (Cd). For this reason, 300 as-hatched, one-day-old broiler chickens were randomly allocated in four dietary treatments with five replicate pens per treatment. Chickens in T1 treatment, were offered a diet supplemented with 0.3 ppm Se (as Se-yeast), without added Cd; in T2 treatment, they were offered a diet with 0.3 ppm Se and 10 ppm Cd; in T3 treatment, they were offered a diet with 0.3 ppm Se and 100 ppm Cd; in T4 treatment, chickens were offered a diet supplemented with 3 ppm Se and 100 ppm Cd. Cadmium was added to the diets in T2, T3, and T4 as CdCl2. On the fourth and sixth weeks, liver and breast samples were obtained from two broilers per replicate pen. Relative gene expression levels of catalase (CAT), superoxide dismutase 1 (SOD1) and 2 (SOD2), methionine sulfoxide reductase A (MSRA) and B3 (MSRB3), iodothyronine deiodinase 1 (DIO1), 2 (DIO2), and 3 (DIO3), glutathione peroxidase 1 (GPX1) and 4 (GPX4), thioredoxin reductase 1 (TXNRD1) and 3 (TXNRD3), and metallothionein 3 (MT3) were analyzed by real-time quantitative PCR in liver, whereas the fatty-acid (FA) profile of breast muscle was determined by gas chromatography. Broilers supplemented with 0.3 ppm Se could tolerate low levels of Cd present in the diets, as there were no significant changes in the breast muscle FA profile, whereas excess Cd led to decreased polyunsaturated fatty acids (PUFAs), and in particular n-6 PUFA. Furthermore, treatments mainly affected the messenger RNA (mRNA) expression of SOD2, TXNRD3, and MT3, while age affected CAT, MSRB3, DIO2, DIO3, GPX4, TXNRD1, and MT3. In conclusion, dietary Se may help against the negative effects of Cd, but cannot be effective when Cd is present at excessive amounts in the diet.

scholarly journals Redox-mediated regulation of a labile, evolutionarily conserved cross-β structure formed by the TDP43 low complexity domain

2019 ◽  
Author(s):  
Yi Lin ◽  
Xiaoming Zhou ◽  
Masato Kato ◽  
Daifei Liu ◽  
Sina Ghaemmaghami ◽  
...  
Keyword(s):  
Rna Binding ◽  
Thioredoxin Reductase ◽  
Methionine Sulfoxide ◽  
Low Complexity ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Residue ◽  
Low Concentrations ◽  
Evolutionarily Conserved ◽  
Methionine Residues ◽  
Self Association

SummaryAn evolutionarily conserved low complexity (LC) domain is found within a 152 residue segment localized to the carboxyl-terminal region of the TDP43 RNA-binding protein. This TDP43 LC domain contains ten conserved methionine residues. Self-association of this domain leads to the formation of liquid-like droplets composed of labile, cross-β polymers. Exposure of polymers to low concentrations of H2O2 leads to a phenomenon of droplet melting that can be reversed upon exposure of the oxidized protein to the MsrA and MsrB methionine sulfoxide reductase enzymes, thioredoxin, thioredoxin reductase and NADPH. Morphological features of the cross-β polymers were revealed by a method of H2O2-mediated footprinting. Similar TDP43 LC domain footprints were observed in highly polymerized, hydrogel samples, liquid-like droplet samples, and living cells. The ability of H2O2 to impede cross-β polymerization was abrogated by a prominent ALS-causing mutation that changes methionine residue 337 to valine. These observations offer potentially useful insight into the biological role of TDP43 in facilitating synapse-localized translation, as well as aberrant aggregation of the protein in neurodegenerative disease.

scholarly journals Effects of the replacement of corn oil with linseed oil on fatty acid composition and the expression of lipogenic genes in broiler chickens

2014 ◽  
Vol 59 (No. 8) ◽  
pp. 353-364 ◽  
Author(s):  
W. Chen ◽  
R. Zhao ◽  
B.X. Yan ◽  
J.S. Zhang ◽  
Y.Q. Huang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Broiler Chickens ◽  
Corn Oil ◽  
Linseed Oil ◽  
Abdominal Fat ◽  
Breast Muscle ◽  
Feed Conversion ◽  
Dietary Pufa ◽  
Total N ◽  
Lipogenic Genes

The effect of dietary 18:2n-6/18:3n-3 ratio (by the replacement of corn oil with linseed oil) on n-3 polyunsaturated fatty acids (PUFA) enrichment in breast muscle of broiler chickens and the expression of lipogenic genes were investigated. Broiler chickens were fed ad libitum with diets containing 5% corn oil (CO), 3.75% corn oil + 1.25% linseed oil (CL1), 2.5% corn oil + 2.5% linseed oil (CL2), and 5% linseed oil (LO) based on the basic diets, respectively. Dietary 18:2n-6/18:3n-3 ratio did not affect 42-day body weight and 0–42-day feed conversion efficiency (feed/gain, P > 0.05) of broiler chickens, however, 5% linseed oil significantly increased 0–21-day feed conversion efficiency (feed/gain, P < 0.05) and decreased breast muscle weight (by 16%, P < 0.05) of broiler chickens. With the decrease of dietary 18:2n-6/18:3n-3 ratio, the enrichment of total n-3 PUFA, 18:3n-3, 20:5n-3, and 22:5n-3 increased linearly (P < 0.01), while the enrichment of total n-6 PUFA and 18:2n-6 decreased linearly (P < 0.01) in breast muscle of broiler chickens. Dietary corn oil increased the enrichment proportion of 20:4n-6 in a dosage-independent manner. Replacing 1.5% corn oil with linseed oil increased the enrichment proportion of 22:6n-3 (P < 0.05), but continuing to increase dietary linseed oil could not further elevate its deposition. Real-time quantitative RT-PCR was used to determine the expression of the mRNA levels of related genes. Dietary PUFA had insignificant effect on the expressions of LPIN2, WD and tetratricopeptide repeats 1 (WDTC1) and △-6 fatty acid desaturase (FADS2) in both breast muscle and abdominal fat. The effect of dietary PUFA on the expression of LPIN1 gene showed clear tissue dependence. Equivalent adding of corn oil and linseed oil could up-regulate the mRNA level of LPIN1 in abdominal fat (P < 0.01). This study demonstrated that decreasing dietary 18:2n-6/18:3n-3 ratio promoted the deposition of desirable n-3 long chain PUFA in the edible tissue and influenced the expression of LPIN1 in a tissue-dependent manner.  

Dietary supplementation with L-arginine and combinations of different oil sources beneficially regulates body fat deposition, lipogenic gene expression, growth performance and carcass yield in broiler chickens

2020 ◽  
Vol 60 (11) ◽  
pp. 1409 ◽  
Author(s):  
J. Khatun ◽  
T. C. Loh ◽  
H. Akit ◽  
H. L. Foo ◽  
R. Mohamad ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Abdominal Fat ◽  
Fat Deposition ◽  
Carcass Yield ◽  
Lipogenic Gene ◽  
Lipogenic Gene Expression

Context Broiler meat with excessive of fat and saturated fatty acids content has serious health implication for consumers. The accumulation of abdominal fats in broiler chickens constitutes a loss of dietary energy and also reduces carcass yield. Oil rich in unsaturated fatty acids and l-arginine are effective for reducing fat deposition and improve meat quality. Aims The aim of this study was to examine the effects of supplementation of l-arginine (l-Arg) with four combinations of palm oil (PO) and sunflower oil (SO) on growth performance, carcass yield, fat deposition, lipogenic gene expression and blood lipid profile in broiler chickens. Methods A total of 180 1-day-old chicks (Cobb 500) were randomly assigned to five dietary treatments as: T1, 6% PO (control); T2, 6% PO + 0.25% l-Arg; T3, 4% PO + 2% SO + 0.25% l-Arg; T4, 2% PO + 4% SO + 0.25% l-Arg; and T5, 6% SO + 0.25% l-Arg. Key results Birds fed l-Arg and combinations of PO and SO had higher weight gain at starter and finisher period compared with the control. The carcass yield increased, and relative abdominal fat reduced in broiler fed with combinations of l-Arg and increased level of SO in the diet. The concentration of oleic, palmitoleic and total monounsaturated fatty acids in liver tissue decreased by addition of l-Arg in broiler diet. The palmitic and total saturated fatty acid decreased, and total unsaturated fatty acid and polyunsaturated fatty acids increased in liver tissue when PO replaced progressively by SO supplemented with l-Arg in the diet. The acetyl-CoA carboxylase , stearoyl-CoA desaturase and fatty acid synthetase gene expression tended to decrease by supplementation of l-Arg with an increased level of SO compared with control. Conclusion Supplementation with l-Arg and combination of PO and SO at the ratio of 4:2 could inhibit lipogenesis and subsequent lower abdominal fat deposition and enhance growth performance and carcass yield in broiler chickens. Implications Ratio of PO and SO, 4:2 with l-Arg supplementation in the dietary of broiler chickens can contribute to a better growth performance, lesser fat deposition and greater carcass yield.

scholarly journals Insect Oil as An Alternative to Palm Oil and Poultry Fat in Broiler Chicken Nutrition

Animals ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 116 ◽  
Author(s):  
Abdelbasset Benzertiha ◽  
Bartosz Kierończyk ◽  
Mateusz Rawski ◽  
Paweł Kołodziejski ◽  
Magdalena Bryszak ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Palm Oil ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Pancreatic Enzyme ◽  
Nutrient Digestibility ◽  
Breast Muscle ◽  
Lipid Fatty Acid ◽  
Muscle Tissues ◽  
Poultry Fat

This study was conducted to evaluate the effects of Tenebrio molitor (TM) oil as a total replacement for palm oil and poultry fat in broiler chicken diets on growth performance, nutrient digestibility, pancreatic enzyme activity, selected blood parameters and the lipid fatty acid compositions of liver and breast muscle tissues. A total of 72 seven-day-old female Ross 308 broiler chickens were used. The birds were randomly distributed into three groups with 12 replicates each, using two birds per replicate for 30 days in metabolic cages. The basal diet was supplemented with 5% palm oil, poultry fat or TM oil. There was no effect (p > 0.05) caused by the dietary oil replacement on the birds’ performance and apparent nutrient digestibility. Liver size (p = 0.033), the concentration of hepatic triglycerides (p = 0.049) and total cholesterol (p = 0.048) were reduced by TM oil supplementation. Furthermore, TM oil supplementation increased n-3 and n-6 fatty acids (p = 0.006; p < 0.001, respectively) in breast muscle tissue. In conclusion, the use of TM oil in broiler chickens’ diets did not show any adverse effects on performance, nutrient digestibility and blood biochemical parameters. Moreover, TM oil supplementation improved the fatty acid profiles of liver and breast muscle tissues.

scholarly journals Selenoproteins and their emerging roles in signaling pathways

2020 ◽  
Vol 11 (2) ◽  
pp. 186-199
Author(s):  
N. V. Stanishevska
Keyword(s):  
Endoplasmic Reticulum ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Thioredoxin Reductase ◽  
Methionine Sulfoxide ◽  
Mapk Signaling ◽  
Methionine Sulfoxide Reductase ◽  
Selenoprotein S ◽  
Selenoprotein K ◽  
Er Membrane

The functional activity of selenoproteins has a wide range of effects on complex pathogenetic processes, including teratogenesis, immuno-inflammatory, neurodegenerative. Being active participants and promoters of many signaling pathways, selenoproteins support the lively interest of a wide scientific community. This review is devoted to the analysis of recent data describing the participation of selenoproteins in various molecular interactions mediating important signaling pathways. Data processing was carried out by the method of complex analysis. For convenience, all selenoproteins were divided into groups depending on their location and function. Among the group of selenoproteins of the ER membrane, selenoprotein N affects the absorption of Ca2+ by the endoplasmic reticulum mediated by oxidoreductin (ERO1), a key player in the CHOP/ERO1 branch, a pathogenic mechanism that causes myopathy. Another selenoprotein of the ER membrane selenoprotein K binding to the DHHC6 protein affects the IP3R receptor that regulates Ca2+ flux. Selenoprotein K is able to affect another protein of the endoplasmic reticulum CHERP, also appearing in Ca2+ transport. Selenoprotein S, associated with the lumen of ER, is able to influence the VCP protein, which ensures the incorporation of selenoprotein K into the ER membrane. Selenoprotein M, as an ER lumen protein, affects the phosphorylation of STAT3 by leptin, which confirms that Sel M is a positive regulator of leptin signaling. Selenoprotein S also related to luminal selenoproteins ER is a modulator of the IRE1α-sXBP1 signaling pathway. Nuclear selenoprotein H will directly affect the suppressor of malignant tumours, p53 protein, the activation of which increases with Sel H deficiency. The same selenoprotein is involved in redox regulation. Among the cytoplasmic selenoproteins, abundant investigations are devoted to SelP, which affects the PI3K/Akt/Erk signaling pathway during ischemia/reperfusion, is transported into the myoblasts through the plasmalemma after binding to the apoER2 receptor, and into the neurons to the megaline receptor and in general, selenoprotein P plays the role of a pool that stores the necessary trace element and releases it, if necessary, for vital selenoproteins. The thioredoxin reductase family plays a key role in the invasion and metastasis of salivary adenoid cystic carcinoma through the influence on the TGF-β-Akt/GSK-3β pathway during epithelial-mesenchymal transition. The deletion of thioredoxin reductase 1 affects the levels of messengers of the Wnt/β-catenin signaling pathway. No less studied is the glutathione peroxidase group, of which GPX3 is able to inhibit signaling in the Wnt/β-catenin pathway and thereby inhibit thyroid metastasis, as well as suppress protein levels in the PI3K/Akt/c-fos pathway. A key observation is that in cases of carcinogenesis, a decrease in GPX3 and its hypermethylation are almost always found. Among deiodinases, deiodinase 3 acts as a promoter of the oncogenes BRAF, MEK or p38, while stimulating a decrease in the expression of cyclin D1. The dependence of the level of deiodinase 3 on the Hedgehog (SHH) signaling pathway is also noted. Methionine sulfoxide reductase A can compete for the uptake of ubiquitin, reduce p38, JNK and ERK promoters of the MAPK signaling pathway; methionine sulfoxide reductase B1 suppresses MAPK signaling messengers, and also increases PARP and caspase 3.

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