scholarly journals Chrysin Reduces Oxidative Stress but Does Not Affect Polyol Pathway in the Lenses of Type 1 Diabetic Rats

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 160 ◽  
Author(s):  
Weronika Wojnar ◽  
Maria Zych ◽  
Sławomir Borymski ◽  
Ilona Kaczmarczyk-Sedlak
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Antioxidative Activity ◽  
Polyol Pathway ◽  
Diabetic Rats ◽  
Stress Index ◽  
Glycation End Products ◽  
Oxidative Damage To Proteins ◽  
And Oxidative Stress

Prolonged hyperglycemia is one of the main causes of reactive oxygen species and free radicals generation in diabetes which may affect various organs, including the eye. Oxidative damage to proteins and lipids in the eye lens could lead to cataract formation. To cope with oxidative stress, the endogenous antioxidative system may be supported by the supplementation of exogenous antioxidants. The aim of this study was to evaluate the effect of chrysin, a natural flavonoid, on oxidative stress and polyol pathway-related markers in the lenses of streptozotocin-induced type 1 male diabetic rats. Chrysin at doses of 50 and 100 mg/kg was administered by gavage for 28 days. This treatment resulted in a decrease in antioxidative enzymes activity and oxidative stress index. Moreover, chrysin administration elevated the reduced glutathione level in the lenses. A decrease in the markers linked to oxidative damage to proteins and lipids in the lenses was noted, especially after treatment with 50 mg/kg of chrysin. Neither of the chrysin doses affected glycemia-related markers in the serum or altered parameters related to the polyol pathway and advanced glycation end-products level in the lenses of diabetic rats. Upon obtaining results, it can be concluded that chrysin reveals antioxidative activity in the lenses but shows no antihyperglycemic or antiglycation properties.

scholarly journals Beneficial Effect of Aqueous Garlic Extract on Inflammation and Oxidative Stress Status in the Kidneys of Type 1 Diabetic Rats

2016 ◽  
Vol 32 (3) ◽  
pp. 329-336 ◽  
Author(s):  
Abolfazl Nasiri ◽  
Nasrin Ziamajidi ◽  
Roghayeh Abbasalipourkabir ◽  
Mohammad Taghi Goodarzi ◽  
Massoud Saidijam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Beneficial Effect ◽  
Diabetic Rats ◽  
Garlic Extract ◽  
Oxidative Stress Status ◽  
And Oxidative Stress

scholarly journals Curcumin attenuates oxidative stress in liver in Type 1 diabetic rats

2017 ◽  
Vol 12 (1) ◽  
pp. 452-459 ◽  
Author(s):  
Zhenglu Xie ◽  
Xinqi Zeng ◽  
Xiaqing Li ◽  
Binbin Wu ◽  
Guozhi Shen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Polyol Pathway ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Curcumin Treatment ◽  
Kinase C ◽  
Diabetic Model ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Plasma Malondialdehyde

AbstractWe investigated the effect of curcumin on liver anti-oxidative stress in the type 1 diabetic rat model induced by streptozotocin (STZ). Experimental diabetic rats were induced by STZ intraperitoneally. All rats were fed for 21 days including three groups of control (NC), diabetic model (DC) and curcumin-treated (Cur, 1.5 g/kg by gavage). The results showed that curcumin-treatment significantly decreased the blood glucose and plasma malondialdehyde levels, but significantly increased the plasma superoxide dismutase, glutathione peroxidase and reduced glutathione levels. Curcumin treatment decreased the activity of aldose reductase, but increased the plasma glucose-6-phosphate dehydrogenase, glucose synthetase and glucose-polymerizing activities. Curcumin treatment significantly decreased the protein of protein kinase C (PKC) and poly ADP ribose polymerase (PARP) expression in the Cur group compared with the DC group. Moreover, the sorbitol dehydrogenase activity was significantly decreased and deterred glucose enters into the polyol pathway leading to an increased NADPH content in the Cur group compared with the DC group. Our data provides evidence that oxidative stress in diabetic rats may be attenuated by curcumin by inhibiting polyol pathway associated with down-regulated expression of PKC and PARP, as evidenced by both an increase the antioxidant enzymes levels and glycogen biosynthesis enzymes activities.

Testosterone promotes glucose intolerance, lipid disorder and oxidative stress in type 1 diabetic rats

2014 ◽  
Vol 25 (1) ◽  
Author(s):  
Ayodele Olufemi Morakinyo ◽  
Daniel Abiodun Adekunbi ◽  
Kayode Akanni Dada ◽  
Olufeyi Adefunke Adegoke
Keyword(s):  
Oxidative Stress ◽  
Glucose Intolerance ◽  
Diabetic Rats ◽  
Lipid Disorder ◽  
And Oxidative Stress

scholarly journals Effects of Metformin in Combination with a Herbal Capsule (Glucoblock) on Insulin Resistance and Oxidative Stress Index in Type 2 Diabetic Rats

2021 ◽  
pp. 18-25
Author(s):  
Ojoye N. Briggs ◽  
Kemzi N. Elechi-amadi ◽  
Justice C. Ohaka ◽  
Edna O. Nwachuku ◽  
Bartimaeus S. Ebirien-agana
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Stress Index ◽  
Control Group ◽  
Oxidative Stress Index ◽  
Treatment Groups ◽  
And Oxidative Stress

Aim: This study evaluated the effects of metformin in combination with a herbal capsule (glucoblock) on insulin resistance and oxidative stress index in type 2 diabetic rats. Methodology: A total of 35 male Wistar albino rats weighing between 120-220 g were used for this study. The rats were placed on high fat diet, and diabetes was induced by a single intraperitoneal injection of freshly prepared streptozotocin (STZ) (45 mg/kg body wt). Fasting plasma glucose (FPG) was determined using the glucose oxidase method. Fasting plasma insulin (FPI), total oxidant status (TOS), total antioxidant status (TAS) and superoxide dismutase (SOD) levels were quantitatively determined by a rat-specific sandwich-enzyme linked immunosorbent assay (ELISA) method. Insulin resistance (IR) was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) method. Oxidative stress index (OSI) was determined by the ratio of TOS to TAS. Phytochemical analysis on the herbal capsule was done using classical methods. Results: The results revealed the presence of alkaloids (100.31μg/mg), flavonoids (131.45μg/mg), cardiac glycosides (55.93μg/mg) and saponins (61.47μg/mg) in the herbal drug glucoblock. The results showed significantly lower FPG levels in the treatment groups when compared to the diabetic control. Group 3 administered metformin had significantly higher FPG levels compared to the negative control. Group 4 administered the herbal drug glucoblock and group 5 administered a combination of metformin and glucoblock, showed no significant differences in FPG levels when compared to the negative control. The diabetic control had significantly higher FPI levels compared to the negative control and treatment groups. The treatment groups showed no significant differences in FPI levels when compared to the negative control. HOMA-IR was significantly higher in the diabetic control compared to the negative control and treatment groups. Also, HOMA-IR values in the treatment groups showed no significant difference compared to the negative control except for group 3 (metformin), that was significantly higher than the negative control. SOD was significantly lower in the diabetic control, compared to the negative control and treatment groups. There were no significant differences in SOD levels in the treatment groups compared to the negative control. TOS levels in the negative control group and treatment groups were significantly lower, compared to the diabetic control. TAS was significantly lower in the diabetic control and treatment groups compared to the negative control. OSI in the diabetic control was significantly higher, compared to the negative control and treatment groups. Also, the treatment groups had significantly higher OSI compared to the negative control. Conclusion: High fat diet and streptozotocin induction produced significant insulin resistance and oxidative stress in the diabetic rats. Glucoblock was more effective in reducing insulin resistance compared to metformin. The combination showed synergistic drug-herb reaction as glucoblock potentiated the actions of metformin. Both showed antioxidant potential but were not effective in lowering oxidative stress to normal levels. There is need to incorporate antioxidant therapy in the treatment protocol for diabetes mellitus.

Abstract 412: The Sodium-Glucose Cotransporter 2 Inhibitor Empagliflozin Improves Diabetic Complications in the Streptozotocin Type 1 Diabetes Mellitus Model by Interfering With Glucotoxicity and Rescue of Beta-Cell Function

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
Philipp Welschof ◽  
Matthias Oelze ◽  
Swenja Kröller-Schön ◽  
Thomas Jansen ◽  
Michael Hausding ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Type I Diabetes ◽  
Cardiovascular Complications ◽  
Diabetic Rats ◽  
Low Grade ◽  
Type I ◽  
Urinary Glucose ◽  
And Oxidative Stress

Objectives: In diabetes, cardiovascular complications are associated with endothelial dysfunction and oxidative stress. Empagliflozin (Empa), as a selective sodium-glucose co-transporter 2 inhibitor (SGLT2i) in clinical development, offers a promising novel approach for the treatment of type 2 diabetes by enhancing urinary glucose excretion. The aim of the present study was to test whether treatment with Empa could improve endothelial dysfunction in type I diabetic rats via reduction of glucotoxicity and associated oxidative stress. Research Design and Methods: Type I diabetes in Wistar rats was induced by an intravenous injection of streptozotocin (60 mg/kg). One week after injection Empa was administered via drinking water for 7 weeks. Results: Treatment with Empa (10 and 30 mg/kg/d), showed reduction of blood glucose and a normalization of endothelial dysfunction (aortic rings) in diabetic rats and a reduced oxidative stress in aortic vessels (dihydroethidine staining) and in blood (phorbol ester/zymosan A-stimulated chemiluminescence). Additionally, the pro-inflammatory phenotype and glucotoxicity in diabetic animals was normalized by SGLT2i therapy. Conclusion: In this study we could demonstrate that Empa improves hyperglycemia and prevents the development of endothelial dysfunction and oxidative stress in type 1 diabetic rats. Future studies will investigate the underlying mechanisms of these antioxidant and anti-inflammatory effects with special emphasis on low-grade inflammation, glucotoxicity and oxidative stress, all of which contributes to cardiovascular complications.

scholarly journals Ameolirative effects of garlic (Allium sativum L.,) in streptozotocin induced oxidative stress in diabetic brain of rat

2020 ◽  
Vol 11 (4) ◽  
pp. 7950-7957
Author(s):  
Pratap Reddy K ◽  
Bhaskar Nagilla ◽  
Varija K
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Polyol Pathway ◽  
Diabetic Rats ◽  
Garlic Extract ◽  
Advanced Glycation ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Glycation End Products ◽  
End Products

The aim of the present study was to evaluate the protective effect of methanol garlic extract on the enzymes related with polyol pathway, advanced glycation end products, markers of oxidative stress and antioxidant status in brain of streptozotocin induced diabetic rats. Antioxidant capability of methanol extract of garlic was evaluated by 2,2-diphenyl-2-picrylhydrazyl hydrate radical and FOX (ferrous ion oxidation-xylenol orange) H2O2 scavenging test. Diabetes was induced by single i.p injection of STZ (32mg/kg per body Wt.,). Blood glucose levels and body wt, were measured on every 7th day over a period of 30 days. The diabetic rats treated with garlic extract at two doses 250mg/kg and 500mg/kg body wt., by oral administration. Diabetic rats showed significant increase in food and water intake, decrease in blood glucose levels, body weights, but could not show any recovery by garlic treatment. Garlic treatment significantly decreased aldose reductase (AR); sorbitol dehydrogenase (SD) and glutathione S-transferase (GST) enzyme activities. A decrease of malndialdehyde (MDA), Protein carbonyls (PC), Pentosidine advance oxidation protein products (AOPP), Advanced glycation end products (AGEs) was also observed. Additionally garlic administration produced a restoration of brain superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) in diabetic rats. Garlic treatment also reduced the free radical formation and progression of hyperglycemia induced diabetic complications by decreasing influx of glucose into the polyol pathway and increased activity of antioxidant enzymes. The current study reveals exerts, efficiently, an attenuating effect of methanol garlic extract exterted antihyperlgycemic, antioxidant and anti-glycating effects in a dose dependent manner in diabetic rats.

Syzygium aromaticum Reduces Diabetes-induced Glucotoxicity via the NRF2/Glo1 Pathway

Planta Medica ◽  
2020 ◽  
Vol 86 (12) ◽  
pp. 876-883
Author(s):  
Moon Ho Do ◽  
Jiwon Choi ◽  
Yoonsook Kim ◽  
Sang Keun Ha ◽  
Guijae Yoo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Damage ◽  
Treated Group ◽  
Diabetic Rats ◽  
Related Factor ◽  
Syzygium Aromaticum ◽  
Inhibitory Effects ◽  
Collagen Crosslinks ◽  
Glycation End Products ◽  
And Oxidative Stress

AbstractAdvanced glycation end products and methylglyoxal are known to show increased levels in diabetic conditions and induce diverse metabolic disorders. However, the antiglycation ability of the bark of Syzygium aromaticum is not yet studied. In this study, we determined the inhibitory effects of S. aromaticum on AGE formation. Moreover, S. aromaticum showed breakage and inhibitory ability against the formation of AGE-collagen crosslinks. In SV40 MES13 cells, treatment with the S. aromaticum extract significantly ameliorated MG-induced oxidative stress as well as cytotoxicity. Furthermore, in the S. aromaticum extract-treated group, there was a reduction in levels of several diabetic markers, such as blood glucose, kidney weight, and urinary albumin to creatinine ratio in streptozotocin-induced diabetic rats. Treatment with the S. aromaticum extract significantly increased the expression of nuclear factor erythroid 2-related factor 2, a transcription factor involved in the expression of antioxidant enzymes. Moreover, the treatment significantly upregulated the expression of glyoxalase 1 and downregulated the expression of receptor for AGEs. These results suggest that the S. aromaticum extract might ameliorate diabetes-induced renal damage by inhibiting the AGE-induced glucotoxicity and oxidative stress through the Nrf2/Glo1 pathway.

scholarly journals Effect of moderate exercise on renal changes and oxidative stress in ovariectomized rats with type 1 diabetes mellitus

2019 ◽  
Vol 6 (13) ◽  
pp. 331-345 ◽  
Author(s):  
Mayane Oliveira Rebouças da Silveira ◽  
Liliany Souza de Brito Amaral ◽  
Samira Itana de Souza ◽  
Halanna Rocha Ferraz ◽  
Jéssica Alves Dias ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Renal Function ◽  
Aerobic Exercise ◽  
Type 1 Diabetes Mellitus ◽  
Diabetic Rats ◽  
Ovariectomized Rats ◽  
And Oxidative Stress

This study evaluated the aerobic exercise effects of moderate and progressive intensity on renal function and structure, and oxidative stress in ovariectomized rats with type 1 diabetes mellitus induced by streptozotocin (STZ). Eighteen Wistar rats were divided into 3 groups: OSC - ovariectomized and sedentary control rats; OSD - ovariectomized and sedentary diabetic rats; and OTD - ovariectomized and trained diabetic rats. After induction of diabetes, the OTD group was submitted to eight weeks of exercise. Twenty-four hours after the last training session urine samples were collected. Blood samples and kidneys were collected after euthanasia for renal function analysis, histology, morphometry and oxidative stress. Our results have shown a reduction of the weight gain, increase of kidney weight and postprandial glycemia in diabetic rats. However, exercise decreased glycosuria and prevented the proteinuria in OTD group rats. Focal segmental glomerulosclerosis (FSGS), juxtamedullary glomerular tuft area, tubulointerstitial lesions (TIL), brush border loss and tubular cell debridement were reduced in OTD rats. In addition, exercise training decreased urinary and plasma concentrations of thiobarbituric acid reactive substance (TBARS). Our results demonstrate the beneficial effect of progressive aerobic exercise on proteinuria, glycosuria, and renal structure in ovariectomized diabetic rats, which may be mediated in part by reduction of oxidative stress.

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