scholarly journals Phytochemical Composition, Antioxidant and Antiproliferative Activities of Defatted Sea Buckthorn (Hippophaë rhamnoides L.) Berry Pomace Fractions Consecutively Recovered by Pressurized Ethanol and Water

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 274 ◽  
Author(s):  
Lijana Dienaitė ◽  
Audrius Pukalskas ◽  
Milda Pukalskienė ◽  
Carolina V. Pereira ◽  
Ana A. Matias ◽  
...  
Keyword(s):  
Functional Foods ◽  
Phenolic Content ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Antiproliferative Effects ◽  
Phytochemical Composition ◽  
Ht29 Cells ◽  
Caco2 Cells ◽  
Potential Applications ◽  
Dpph Scavenging

This study aimed at valorisation of sea buckthorn pomace (SBP) for the production of extracts containing valuable bioactive compounds. For this purpose, SBP defatted by supercritical CO2 was subjected to consecutive fractionation with pressurized ethanol and water, which yielded 11.9% and 4.8% of extracts, respectively. The extracts were evaluated for their antioxidant potential, phytochemical composition and antiproliferative effects against cancer cells. Water extracts exhibited remarkably higher values in Folin-Ciocalteu assay of total phenolic content, oxygen radical absorbance capacity (ORAC), ABTS●+/DPPH● scavenging and cellular antioxidant activity (CAA) assays and more efficiently inhibited proliferation of HT29 cells at non-cytotoxic concentrations measured in non-tumoral Caco2 cells. Among 28 detected and 21 quantified phytochemicals, flavonols with the structures of isorhamnetin (five compounds), quercetin (three compounds), kaempferol (three compounds) glycosides and catechin (six compounds) were the most abundant in the extracts. In conclusion, the applied method of fractionation of SBP produces promising natural antioxidant complexes with antiproliferative properties that could find potential applications in nutraceuticals, functional foods and cosmeceuticals.

scholarly journals Influence of Seasonality on Phytochemical Composition, Phenolic Content and Antioxidant Activity of Neoglaziovia variegata (Bromeliaceae)

2021 ◽  
Vol 12 (3) ◽  
pp. 2889-2904
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Content ◽  
Principal Component ◽  
Total Phenolic ◽  
Phytochemical Composition ◽  
Potential Applications ◽  
Anthracene Derivatives ◽  
Layer Chromatography

The production of phytochemicals in plants can be influenced by several factors. This work evaluated the influence of seasonality on phytochemical composition, phenolic content, and antioxidant activity of Neoglaziovia variegata. The leaves were harvested for a year. Phytochemical characterization by thin-layer chromatography indicated the presence of anthocyanins, anthraquinones, anthracene derivatives, flavonoids, tannins, monoterpenes, diterpenes, triterpenes, and steroids in the leaves collected in all months, and coumarins in leaves of 4 of the 12 months of collection. The antioxidant activity, evaluated in vitro, ranged from 33.0 to 86.1% inhibition of DPPH• and from 36.2 to 97.8% inhibition of ABTS•+. The total phenolic content, determined using Folin-Ciocalteu reagent, varied between 21.5 and 65.8 mg GAE/g. Phenolic acids and flavonoids were determined by HPLC-DAD, with higher concentrations of kaempferol (0.47-3.57 mg/g), p-coumaric (0.19-3.59 mg/g) and ellagic (0.26-3.31 mg/g) acids. The Principal Component Analysis (PCA) indicated the separation of the leaf samples according to the season. It was demonstrated the influence of seasonality on the antioxidant activity and phytochemical composition of N. variegata leaves and that this species is rich in secondary metabolites of different classes, with potential applications in several areas, such as pharmaceutical and food.

scholarly journals Raspberry and Strawberry Addition Improves Probiotic Viability in Yogurt and Possess Antioxidant Activity

2015 ◽  
Vol 4 (4) ◽  
pp. 47 ◽  
Author(s):  
Aynur Gunenc ◽  
Susanna Fang ◽  
Farah Hosseinian
Keyword(s):  
Antioxidant Activity ◽  
Cold Storage ◽  
Functional Foods ◽  
Phenolic Content ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Microbial Counts ◽  
Total Titratable Acidity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

<p>This study aimed to i) investigate probiotic potentials of raspberry and strawberry addition in yogurts, ii) explore antioxidant activity of berries extracted by microwave using oxygen radical absorbance (ORAC), and 2,2-diphenyl-1-picryhydrazyl radical (DPPH) as well as iii) determine the total phenolic content (TPC) of the berries. The probiotic potentials of those berry additions into yogurts containing different probiotics were determined by subsequent viable microorganism counts in each yogurt trial using selective media, pH and total titratable acidity (TTA) during 28 days of cold storage at 4ºC. Viable microbial counts in yogurt trials containing probiotic <em>Lactobacillus acidophilus </em>and raspberry increased (P&lt;0.05) for 21 consecutive days of cold storage. The pH levels decreased (P&lt;0.05) as the TTA increased over 28 days of cold storage in all yogurts containing the berries. ORAC results showed that raspberry had higher antioxidant activity (505.72 µmol TE/100g of fruit) than strawberry (495 µmol TE/100g of fruit). Also, DPPH scavenging activity results showed that raspberry (86.11%) had higher antioxidant activity than strawberry (85.69%). There was not a significant (P&lt;0.05) difference in TPC values of raspberry (0.20 g GAE/kg) and strawberry (0.18 g GAE/kg). This study suggests that both berries have potential as a source of prebiotics with antioxidant activity for future functional foods and nutraceutical applications.</p>

scholarly journals Valorization of European Cranberry Bush (Viburnum opulus L.) Berry Pomace Extracts Isolated with Pressurized Ethanol and Water by Assessing Their Phytochemical Composition, Antioxidant, and Antiproliferative Activities

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1413 ◽  
Author(s):  
Lijana Dienaitė ◽  
Milda Pukalskienė ◽  
Carolina V. Pereira ◽  
Ana A. Matias ◽  
Petras Rimantas Venskutonis
Keyword(s):  
Phenolic Content ◽  
Total Phenolic ◽  
Healthy Foods ◽  
Viburnum Opulus ◽  
Functional Ingredients ◽  
Phytochemical Composition ◽  
On Line ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay ◽  
Antiproliferative Activities

Defatted by supercritical CO2, Viburnum opulus berry pomace (VOP) was subjected to consecutive extraction with pressurized ethanol (E) and water (W) and yielded 23% of VOP-E and 8% of VOP-W, respectively. The major phytochemical groups covering 42 identified and quantified constituents in VOP extracts were organic and phenolic acids, iridoids, quercetin and (epi)catechin derivatives, flavalignans, procyanidins, and anthocyanins. The on-line HPLC-DPPH•-scavenging assay revealed the presence of numerous antioxidants. VOP-E had a higher total phenolic content, was a stronger antioxidant (equivalent to 0.77, 0.42, and 0.17 g trolox/g in oxygen radical absorbance capacity (ORAC), ABTS, and DPPH assays, respectively), and recovered the major part of phenolics from the pomace; however, both extracts demonstrated similar antioxidant activity in the cellular assay. VOP-E inhibited HT29 cancer cells at non-cytotoxic concentrations. The results of this study revealed that VOP contains valuable phytochemicals possessing antioxidant and antiproliferative activities. Consequently, extracts from VOP substances may be of interest in developing functional ingredients for healthy foods, nutraceuticals, and cosmeceuticals.

scholarly journals ANTIOXIDANT EVALUATION AND PHYTOCHEMICAL CONTENT OF VARIOUS RICE BRAN EXTRACTS OF THREE VARIETIES RICE FROM SEMARANG-CENTRAL JAVA, INDONESIA

2017 ◽  
Vol 10 (6) ◽  
pp. 377 ◽  
Author(s):  
Sukrasno Sukrasno ◽  
Slamet Tuty ◽  
Irda Fidrianny
Keyword(s):  
Rice Bran ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Red Rice ◽  
Black Rice ◽  
Dpph Assay ◽  
Rice Bran Extract ◽  
Dpph Scavenging

Objectives: The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were FRAP (Ferric Reducing Antioxidant Power) and DPPH (2,2-diphenyl-1-picrylhydrazyl), and correlation of total phenolic, flavonoid and carotenoid content with their EC50 of FRAP and IC50 of DPPH antioxidant activities. Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and carotenoid content, antioxidant activities using FRAP and DPPH assays were performed by UV-visible spectrophotometry and its correlation with EC50 of FRAP capacities and IC50 of DPPH scavenging activities were analyzed by Pearson’s method. Results: Ethanolic rice bran extract of black rice showed the lowest EC50 of FRAP capacity 64.35 µg/ml and IC50 of DPPH scavenging activity 23.92 µg/ml. The highest phenolic content, flavonoid content and carotenoid content were also given by ethanolic rice bran extract of black rice. There were significantly negative correlation between total phenolic content and carotenoid content in rice bran extract of red rice and black rice with their IC50 of DPPH. Conclusions: All of rice bran extracts (except n-hexane rice bran extract of black rice and ethanolic rice bran extract of white rice) were very strong antioxidant, by DPPH assay. Phenolic and carotenoid compounds in rice bran extracts of red rice and black rice were the major contributor in antioxidant activity by DPPH assay. Rice bran extracts of black rice had linear results by FRAP and DPPH assays. 

scholarly journals Antioxidant potential of five commercially less valued leafy vegetables from Bangladesh

2018 ◽  
Vol 47 (4) ◽  
pp. 953-959
Author(s):  
Kishwar Jahan Shethi ◽  
Mohammad Zashim Uddin
Keyword(s):  
Phenolic Content ◽  
Leafy Vegetables ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phenolic And Flavonoid

To evaluate the antioxidant potential of less valued green leafy vegetables of Bangladesh, the methanolic extracts of Alternanthera sessilis, A. philoxeroides, A. paronychioides, Glinus oppositifolius and Portulaca oleracea were subjected to assay for total phenolic content, total flavonoid content and DPPH free radicle scavenging activity. Among the studied materials, A. sessilis showed the highest phenolic and flavonoid content. However, G. oppositifolius presented the lowest phenolic content (14.53 ± 0.48 mg of GAE/g of sample) and P. oleracea exhibited minimum (7.005 ± 0.25 mg of RUE/g of sample) flavonoid content. DPPH radical scavenging activity was found to increase with increase in the concentration of each individual material. A. philoxiroides (IC50 = 6.07 ± 0.27 μg/ml) was found as the most active in DPPH radical percentage inhibition, which was followed by A. sessilis (IC50 = 7.17 ± 0.67 μg/ml). Interestingly, A. paronychioides, in spite of having good amount of total phenolic and flavonoid content, showed less DPPH scavenging activity. No linear relationship was found to exist in case of the total phenolic, flavonoid content and DPPH scavenging activity among G. oppositifolius, P. oleracea and A. paronychioides. The results from different methods may differ substantially because each complex chemical reaction generates unique values. However, all the five plants exhibited creditable antioxidant activity and thus consumption of these vegetables may play important role in combating oxidative stresses and thereby help in retaining good health.

scholarly journals Sunflower (Helianthus annuus L.) Plants at Various Growth Stages Subjected to Extraction—Comparison of the Antioxidant Activity and Phenolic Profile

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 535 ◽  
Author(s):  
Francesco Gai ◽  
Magdalena Karamać ◽  
Michał A. Janiak ◽  
Ryszard Amarowicz ◽  
Pier Giorgio Peiretti
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Growth Stages ◽  
Scavenging Activity ◽  
Flowering Stage ◽  
Phenolic Profile ◽  
Aerial Parts ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The aim of this study was to evaluate the differences in the antioxidant activity and phenolic profile of sunflower (Helianthus annuus L.) extracts obtained from the aerial parts of plants harvested at five growth stages. In vitro assays were used to determine the antioxidant activity, i.e., ABTS•+ and DPPH• scavenging activity, the ferric-reducing antioxidant power (FRAP) and the ability to inhibit β-carotene–linoleic acid emulsion oxidation. Phenolic compounds, such as mono- and dicaffeoylquinic acid isomers and caffeic acid hexose, were identified using the LC–TOF–MS/MS technique. The predominant compound during the growth cycle of the plant was 3,5-di-O-caffeoylquinic acid, whose content was the highest at the mid-flowering stage. The total phenolic content was also the highest in sunflowers at the mid-flowering stage. The main phenolic compound contents were closely correlated with ABTS•+ and DPPH• scavenging activity and FRAP. No significant correlation was found between the total phenolic content and the antioxidant activity in the emulsion system. The highest antiradical activity and FRAP were generally determined in older plants (mid-flowering and late flowering stages). In conclusion, the aerial parts of sunflowers, in particular those harvested at the mid-flowering stage, are a good plant material from which to obtain phenolic compound extracts, albeit mainly of one class (esters of caffeic acid and quinic acid), with high antioxidant activity.

scholarly journals Antioxidant Properties of Crude Extract, Partition Extract, and Fermented Medium of Dendrobium sabin Flower

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Farahziela Abu ◽  
Che Norma Mat Taib ◽  
Mohamad Aris Mohd Moklas ◽  
Sobri Mohd Akhir
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Correlation Relationship ◽  
Good Potential ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Antioxidant properties of crude extract, partition extract, and fermented medium from Dendrobium sabin (DS) flower were investigated. The oven-dried DS flower was extracted using 100% methanol (w/v), 100% ethanol (w/v), and 100% water (w/v). The 100% methanolic crude extract showed the highest total phenolic content (40.33 ± mg GAE/g extract) and the best antioxidant properties as shown by DPPH, ABTS, and FRAP assays. A correlation relationship between antioxidant activity and total phenolic content showed that phenolic compounds were the dominant antioxidant components in this flower extract. The microbial fermentation on DS flower medium showed a potential in increasing the phenolic content and DPPH scavenging activity. The TPC of final fermented medium showed approximately 18% increment, while the DPPH of fermented medium increased significantly to approximately 80% at the end of the fermentation. Dendrobium sabin (DS) flower showed very good potential properties of antioxidant in crude extract and partition extract as well as better antioxidant activity in the flower fermented medium.

Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

2011 ◽  
Vol 38 (9) ◽  
pp. 697 ◽  
Author(s):  
Arvind K. Goyal ◽  
Bharat C. Basistha ◽  
Arnab Sen ◽  
Sushil K. Middha
Keyword(s):  
Antioxidant Activity ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Hippophae Salicifolia ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phenolic And Flavonoid

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

scholarly journals Effect of varying levels of acorn flour on antioxidant, staling and sensory properties of Iranian toast

2021 ◽  
Vol 10 (2) ◽  
pp. 322-333
Author(s):  
Babak Mousavi ◽  
Sajad Ghaderi ◽  
Mohammad Ali Hesarinejad ◽  
Azizollah Pourmahmoudi
Keyword(s):  
Functional Foods ◽  
Phenolic Content ◽  
Sensory Properties ◽  
Total Phenolic ◽  
Anti Oxidant ◽  
The Stability ◽  
High Level ◽  
Acorn Flour ◽  
Colour Indices ◽  
Oxidant Content

Due to the high level of antioxidant activity of acorn fruits, they can be used as an ingredient the production of functional foods. The goal of the this research was to partially substitute wheat flour with varying levels (10% - 50% w/w) of debittered acorn flour and to investigate its effects on the rheological characteristics of the dough, total phenolic content (TPC), staling, colour indices and sensory properties of toast bread. The farinograph degree of softening and water absorption of the dough decreased with increasing the acorn flour content, while the stability and time of development of the dough increased. Resistance and extensibility to deformation of the samples respectively increased and decreased compared to those of the control. Toast bread with 30% acorn flour replacement was observed to have lower staling than the control. The highest TPC (9.44 mg GAE/g) and the lowest peroxide value (0.36 m eq O2/kg) were obtained for the bread having 30% acorn flour substitution. Moreover, the breads showed darker crumbs with significantly lower specific loaf volumes. Overall, the bread with 30% of acorn flour substitution showed good rheological, staling properties and reasonable anti-oxidant content compared to the control bread as well as the highest sensorial acceptability.

scholarly journals Antioxidant and Anti-Obesity Potentials of Korean-Native Wild Vegetables (Allium species)

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 541
Author(s):  
Ji Yeon Lee ◽  
Kyung Hye Seo ◽  
Eun Young Lee ◽  
Yun-Jeong Ji ◽  
Yun Ji Lee ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
High Performance ◽  
Phenolic Content ◽  
Therapeutic Potential ◽  
Total Phenolic ◽  
Edible Plants ◽  
Allium Species ◽  
Wild Vegetables ◽  
New Crops ◽  
Dpph Scavenging

Four Korea-native wild vegetables belonging to the Allium genus (Allium tuberosum Rottl., Allium senescens L., Allium thunbergii G. Don., and Allium sacculiferum Maxim.) were investigated for their antioxidant and anti-obesity potential, in order to promote the utilization of wild edible plants for the development of potential new crops. In this study, we compared the antioxidant activity of the plant extracts by ABTS+ and DPPH scavenging method. We then compared the anti-adipogenic effects of the extracts in adipocytes. We quantified the phenolics in the extracts using high-performance liquid chromatography (HPLC) to identify the bioactive compounds. A. tuberosum and A. sacculiferum had a higher total phenolic content (19.0 ± 0.3 and 19.6 ± 0.7 mg GA/g extract, respectively) than A. senescens and A. thunbergii (16.0 ± 0.2 and 14.5 ± 0.7 mg GA/g extract). Caffeic acid, an antioxidant and anti-adipogenic compound, was detected in A. tuberosum and A. sacculiferum, but not in A. senescens and A. thunbergii. A. tuberosum, and A. sacculiferum showed superior antioxidative and anti-adipogenic activities compared to A. senescens and A. thunbergii. Therefore, A. tuberosum and A. sacculiferum, both of which contain antioxidant and anti-adipogenic phenolics, such as caffeic acid, present a therapeutic potential against obesity. Various phenolic compounds, which have been found to differ among Allium spp., may partially account for differences among the Allium spp. with respect to their antioxidant and anti-obesity effects.

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