scholarly journals Preliminary Study on the Development of In Vitro Human Respiratory Epithelium Using Collagen Type I Scaffold as a Potential Model for Future Tracheal Tissue Engineering

2021 ◽  
Vol 11 (4) ◽  
pp. 1787
Author(s):  
Yogeswaran Lokanathan ◽  
Mh Busra Fauzi ◽  
Rohaina Che Man ◽  
Zahra Rashidbenam ◽  
Aminuddin Bin Saim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Collagen Type ◽  
Ex Vivo ◽  
Collagen Type I ◽  
Respiratory Epithelium ◽  
Tracheal Epithelium ◽  
Type I ◽  
Potential Candidate ◽  
3D Scaffold ◽  
Replacement Method

Pathological conditions of the tracheal epithelium, such as postoperative injuries and chronic conditions, often compromise the functionality of the respiratory epithelium. Although replacement of the respiratory epithelium using various types of tracheal transplantation has been attempted, there is no predictable and dependable replacement method that holds for safe and practicable long-term use. Therefore, we used a tissue engineering approach for ex vivo regeneration of the respiratory epithelium (RE) construct. Collagen type I was isolated from sheep tendon and it was fabricated in a three-dimensional (3D) scaffold format. Isolated human respiratory epithelial cells (RECs) and fibroblasts from nasal turbinate were co-cultured on the 3D scaffold for 48 h, and epithelium maturation was allowed for another 14 days in an air–liquid interface culture system. The scanning electron microscope results revealed a fabricated porous-structure 3D collagen scaffold. The scaffold was found to be biocompatible with RECs and fibroblasts and allows cells attachment, proliferation, and migration. Immunohistochemical analysis showed that the seeded RECs and fibroblasts were positive for expression of cytokeratin 14 and collagen type I markers, respectively, indicating that the scaffold supports the native phenotype of seeded cells over a period of 14 days. Although a longer maturation period is needed for ciliogenesis to occur in RECs, the findings suggest that the tissue-engineered RE construct is a potential candidate for direct use in tracheal epithelium replacement or tracheal tube reengineering.

scholarly journals Precision-Cut Kidney Slices as a Tool to Understand the Dynamics of Extracellular Matrix Remodeling in Renal Fibrosis

2016 ◽  
Vol 11 ◽  
pp. BMI.S38439 ◽  
Author(s):  
Federica Genovese ◽  
Zsolt S. Kàrpàti ◽  
Signe H. Nielsen ◽  
Morten A. Karsdal
Keyword(s):  
Extracellular Matrix ◽  
Collagen Type ◽  
Interstitial Fibrosis ◽  
Ex Vivo ◽  
Collagen Type I ◽  
Extracellular Matrix Remodeling ◽  
Type I ◽  
Matrix Remodeling ◽  
Renal Interstitial Fibrosis ◽  
Ex Vivo Model

The aim of this study was to set up an ex vivo model for renal interstitial fibrosis in order to investigate the extracellular matrix (ECM) turnover profile in the fibrotic kidney. We induced kidney fibrosis in fourteen 12-week-old male Sprague Dawley rats by unilateral ureteral obstruction (UUO) surgery of the right ureter. The left kidney (contralateral) was used as internal control. Six rats were sham operated and used as the control group. Rats were terminated two weeks after the surgery; the kidneys were excised and precision-cut kidney slices (PCKSs) were cultured for five days in serum-free medium. Markers of collagen type I formation (P1NP), collagen type I and III degradation (C1M and C3M), and α-smooth muscle actin (αSMA) were measured in the PCKS supernatants by enzyme-linked immunosorbent assay. P1NP, C1M, C3M, and α-SMA were increased up to 2- to 13-fold in supernatants of tissue slices from the UUO-ligated kidneys compared with the contralateral kidneys ( P < 0.001) and with the kidneys of sham-operated animals ( P < 0.0001). The markers could also reflect the level of fibrosis in different animals. The UUO PCKS ex vivo model provides a valuable translational tool for investigating the extracellular matrix remodeling associated with renal interstitial fibrosis.

Fabrication of a RP-Based Biomimetic Grafting Material for Bone Tissue Engineering

2009 ◽  
Vol 626-627 ◽  
pp. 553-558 ◽  
Author(s):  
Xing Ma ◽  
Y.Y. Hu ◽  
Xiao Ming Wu ◽  
J. Liu ◽  
Zhuo Xiong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Type ◽  
Autologous Bone Marrow ◽  
Collagen Type I ◽  
Control Group ◽  
Type I ◽  
High Porosity ◽  
Highly Porous

Three-dimensional (3D) highly porous poly (DL-lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The biopolymer carriers (4mm×4mm×4mm) subsequently were coated with collagen type I (Col) to produce PLGA/TCP/Col composites and utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Autologous bone marrow stromal cells (BMSCs) harvested from New Zealand white rabbits were cultured under an osteogenic condition (BMSCs-OB) followed by seeding into the structural highly porous PLGA/TCP/Col composites (i.e. PLGA/TCP/Col/BMSCs-OB). Scanning electron microscopy observation found that the RP-based scaffolds had appropriate microstructure, controlled interconnectivity and high porosity. Modification of the scaffolds with collagen type I (PLGA/TCP/Col) essentially increased the affinity of the carriers to seeding cells, and PLGA/TCP/Col composites were well biocompatible with BMSCs-OB. The PLGA/TCP/Col/BMSCs-OB constructs were then subcutaneously implanted in the back of rabbits compared to controls with autologous BMSCs suspension and carriers alone. As a result, histological new bone formation was observed only in the experimental group with PLGA/TCP/Col/BMSCs-OB constructs 8 weeks after implantation. In the control group with scaffold alone only biodegradation of the carriers was found. Therefore, these results validate our bio-manufacturing methods for a new bone graft substitute.

scholarly journals Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications

2011 ◽  
Vol 17 (9) ◽  
pp. 879-885 ◽  
Author(s):  
Christina A. Pacak ◽  
Jared M. Powers ◽  
Douglas B. Cowan
Keyword(s):  
Tissue Engineering ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Engineering Applications

scholarly journals Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering

2016 ◽  
Vol 17 (10) ◽  
pp. 3145-3152 ◽  
Author(s):  
Nelda Vázquez-Portalatı́n ◽  
Claire E. Kilmer ◽  
Alyssa Panitch ◽  
Julie C. Liu
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Collagen Type ◽  
Cartilage Tissue Engineering ◽  
Collagen Type I ◽  
Cartilage Tissue ◽  
Type I

Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering

2018 ◽  
Vol 7 (18) ◽  
pp. 1800605 ◽  
Author(s):  
Heinz P. Janke ◽  
Nihan Güvener ◽  
Weiqiang Dou ◽  
Dorien M. Tiemessen ◽  
Anglita YantiSetiasti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Mr Imaging ◽  
Contrast Agent ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Soft Tissue Engineering

scholarly journals Collagen Type I Biomaterials as Scaffolds for Bone Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 599
Author(s):  
Gustavo A. Rico-Llanos ◽  
Sara Borrego-González ◽  
Miguelangel Moncayo-Donoso ◽  
José Becerra ◽  
Rick Visser
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Mechanical Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Type ◽  
Collagen Type I ◽  
Current Status ◽  
Type I ◽  
Organic Constituent

Collagen type I is the main organic constituent of the bone extracellular matrix and has been used for decades as scaffolding material in bone tissue engineering approaches when autografts are not feasible. Polymeric collagen can be easily isolated from various animal sources and can be processed in a great number of ways to manufacture biomaterials in the form of sponges, particles, or hydrogels, among others, for different applications. Despite its great biocompatibility and osteoconductivity, collagen type I also has some drawbacks, such as its high biodegradability, low mechanical strength, and lack of osteoinductive activity. Therefore, many attempts have been made to improve the collagen type I-based implants for bone tissue engineering. This review aims to summarize the current status of collagen type I as a biomaterial for bone tissue engineering, as well as to highlight some of the main efforts that have been made recently towards designing and producing collagen implants to improve bone regeneration.

An improvement of silk-based scaffold properties using collagen type I for skin tissue engineering applications

2017 ◽  
Vol 75 (2) ◽  
pp. 685-700 ◽  
Author(s):  
Suwimon Boonrungsiman ◽  
Nareerat Thongtham ◽  
Orawan Suwantong ◽  
Tuksadon Wutikhun ◽  
Nattakan Soykeabkaew ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Collagen Type ◽  
Collagen Type I ◽  
Skin Tissue ◽  
Type I ◽  
Engineering Applications ◽  
Skin Tissue Engineering ◽  
Scaffold Properties

Osteochondral Autograft Plugs versus Paste Graft: Ex Vivo Morselization Increases Chondral Matrix Production

Cartilage ◽  
2020 ◽  
pp. 194760352091655
Author(s):  
Daniel Grande ◽  
Todd Goldstein ◽  
Thomas J. Turek ◽  
Susan Hennessy ◽  
Ann W. Walgenbach ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Viability ◽  
Collagen Type ◽  
Ex Vivo ◽  
Collagen Type I ◽  
Cartilage Tissue ◽  
Type I ◽  
Osteochondral Autograft ◽  
Qrt Pcr ◽  
Matrix Production

Objective Patients undergoing articular cartilage paste grafting have been shown in studies to have significant improvement in pain and function in long-term follow-ups. We hypothesized that ex vivo impacting of osteochondral autografts results in higher chondrocyte matrix production versus intact osteochondral autograft plugs. Design This institutional review board–approved study characterizes the effects of impacting osteochondral plugs harvested from the intercondylar notch of 16 patients into a paste, leaving one graft intact as a control. Cell viability/proliferation, collagen type I/II, SOX-9, and aggrecan gene expression via qRT-PCR (quantitative reverse transcription-polymerase chain reaction) were analyzed at 24 and 48 hours. Matrix production and cell morphology were evaluated using histology. Results Paste samples from patients (mean age 39.7) with moderate (19%) to severe (81%) cartilage lesions displayed 34% and 80% greater cell proliferation compared to plugs at 24 and 48 hours post processing, respectively ( P = 0.015 and P = 0.021). qRT-PCR analysis yielded a significant ( P = 0.000) increase of aggrecan, SOX-9, collagen type I and II at both 24 and 48 hours. Histological examination displayed cell division throughout paste samples, with accumulation of aggrecan around multiple chondrocyte lacunae. Conclusions Paste graft preparation resulted in increased mobility of chondrocytes by matrix disruption without loss of cell viability. The impaction procedure stimulated chondrocyte proliferation resulting in a cellular response to reestablish native extracellular matrix. Analysis of gene expression supports a regenerative process of cartilage tissue formation and contradicts long-held beliefs that impaction trauma leads to immediate cell death. This mechanism of action translates into clinical benefit for patients with moderate to severe cartilage damage.

scholarly journals A 3D-Printed PLCL Scaffold Coated with Collagen Type I and Its Biocompatibility

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yong He ◽  
Wei Liu ◽  
Lianxiong Guan ◽  
Jielin Chen ◽  
Li Duan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Mechanical Test ◽  
Great Influence ◽  
Collagen Type I ◽  
Blue Staining ◽  
Type I ◽  
3D Printed ◽  
Better Than

Scaffolds play an important role in tissue engineering and their structure and biocompatibility have great influence on cell behaviors. In this study, poly(l-lactide-co-ε-caprolactone) (PLCL) scaffolds were printed by a 3D printing technology, low-temperature deposition manufacturing (LDM), and then PLCL scaffolds were treated by alkali and coated with collagen type I (COLI). The scaffolds were characterized by scanning electron microscopy (SEM), porosity test, mechanical test, and infrared spectroscopy. The prepared PLCL and PLCL-COLI scaffolds had three-dimensional (3D) porous structure and they not only have macropores but also have micropores in the deposited lines. Although the mechanical property of PLCL-COLI was slightly lower than that of PLCL scaffold, the hydrophilicity of PLCL-COLI was significantly enhanced. Rabbit articular chondrocytes were extracted and were identified as chondrocytes by toluidine blue staining. To study the biocompatibility, the chondrocytes were seeded on scaffolds for 1, 3, 5, 7, and 10 days. MTT assay showed that the proliferation of chondrocytes on PLCL-COLI scaffold was better than that on PLCL scaffold. And the morphology of cells on PLCL-COLI after 1-day culture was much better than that on PLCL. This 3D-printed PLCL scaffold coated with COLI shows a great potential application in tissue engineering.

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