scholarly journals Modulation of Metamorphic and Regenerative Events by Cold Atmospheric Pressure Plasma Exposure in Tadpoles, Xenopus laevis

2019 ◽  
Vol 9 (14) ◽  
pp. 2860 ◽  
Author(s):  
Ma Veronica Holganza ◽  
Adonis Rivie ◽  
Kevin Martus ◽  
Jaishri Menon
Keyword(s):  
Atmospheric Pressure ◽  
Developmental Plasticity ◽  
Morphological Changes ◽  
Atmospheric Pressure Plasma ◽  
Ultrastructural Changes ◽  
Plasma Exposure ◽  
Tail Regeneration ◽  
Developmental Processes ◽  
Pressure Plasma ◽  
Metamorphic Development

Atmospheric pressure plasma has found wide clinical applications including wound healing, tissue regeneration, sterilization, and cancer treatment. Here, we have investigated its effect on developmental processes like metamorphosis and tail regeneration in tadpoles. Plasma exposure hastens the process of tail regeneration but delays metamorphic development. The observed differences in these two developmental processes following plasma exposure are indicative of physiological costs associated with developmental plasticity for their survival. Ultrastructural changes in epidermis and mitochondria in response to the stress of tail amputation and plasma exposure show characteristics of cellular hypoxia and oxidative stress. Mitochondria show morphological changes such as swelling with wide and fewer cristae and seem to undergo processes such as fission and fusion. Complex interactions between calcium, peroxisomes, mitochondria and their pore transition pathways are responsible for changes in mitochondrial structure and function, suggesting the subcellular site of action of plasma in this system.

scholarly journals In Situ Investigation of the Formation Kinematics of Plasma-Generated Silver Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 555 ◽  
Author(s):  
Daniel Tasche ◽  
Mirco Weber ◽  
Julia Mrotzek ◽  
Christoph Gerhard ◽  
Stephan Wieneke ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Atmospheric Pressure ◽  
Nanoparticle Synthesis ◽  
Atmospheric Pressure Plasma ◽  
Spherical Particles ◽  
Synthesis Process ◽  
Plasma Exposure ◽  
Pressure Plasma ◽  
In Situ Investigation

In this publication, it is shown how to synthesize silver nanoparticles from silver cations out of aqueous solutions by the use of an atmospheric pressure plasma source. The use of an atmospheric pressure plasma leads to a very fast reduction of silver ions in extensive solvent volumes. In order to investigate the nanoparticle synthesis process, ultraviolet/visible (UV/VIS) absorption spectra were recorded in situ. By using transmission electron microscopy and by the analysis of UV/VIS spectra, the kinetics of silver nanoparticle formation by plasma influence can be seen in more detail. For example, there are two different sections visible in the synthesis during the plasma exposure process. The first section of the synthesis is characterized by a linear formation of small spherical particles of nearly constant size. The second section is predominated by saturation effects. Here, particle faults are increasingly formed, induced by changes in the particle shape and the fusion of those particles. The plasma exposure time, therefore, determines the shape and size distribution of the nanoparticles.

scholarly journals Oxidative modification and electrochemical inactivation of Escherichia coli upon cold atmospheric pressure plasma exposure

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173618 ◽  
Author(s):  
Marlène Dezest ◽  
Anne-Laure Bulteau ◽  
Damien Quinton ◽  
Laurent Chavatte ◽  
Mickael Le Bechec ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Oxidative Modification ◽  
Plasma Exposure ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

scholarly journals The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley

2021 ◽  
Vol 22 (6) ◽  
pp. 2833
Author(s):  
Mária Peťková ◽  
Renáta Švubová ◽  
Stanislav Kyzek ◽  
Veronika Medvecká ◽  
Ľudmila Slováková ◽  
...  
Keyword(s):  
Dna Damage ◽  
Enzyme Activities ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Agricultural Practice ◽  
Plasma Exposure ◽  
Positive Effects ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
Germination Parameters

Climate change, environmental pollution and pathogen resistance to available chemical agents are part of the problems that the food industry has to face in order to ensure healthy food for people and livestock. One of the promising solutions to these problems is the use of cold atmospheric pressure plasma (CAPP). Plasma is suitable for efficient surface decontamination of seeds and food products, germination enhancement and obtaining higher yields in agricultural production. However, the plasma effects vary due to plasma source, treatment conditions and seed type. In our study, we tried to find the proper conditions for treatment of barley grains by diffuse coplanar surface barrier discharge, in which positive effects of CAPP, such as enhanced germination or decontamination effects, would be maximized and harmful effects, such as oxidation and genotoxic potential, minimized. Besides germination parameters, we evaluated DNA damage and activities of various germination and antioxidant enzymes in barley seedlings. Plasma exposure resulted in changes in germination parameters and enzyme activities. Longer exposures had also genotoxic effects. As such, our findings indicate that appropriate plasma exposure conditions need to be carefully optimized in order to preserve germination, oxidation balance and genome stability, should CAPP be used in agricultural practice.

Inactivation of Bread Molds on Agar by Atmospheric Pressure Plasma Jet

2017 ◽  
Vol 866 ◽  
pp. 92-96 ◽  
Author(s):  
Panakamon Thonglor ◽  
Plaimein Amnuaycheewa
Keyword(s):  
Atmospheric Pressure ◽  
Argon Plasma ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Dry Weight ◽  
Atmospheric Pressure Plasma Jet ◽  
Plasma Exposure ◽  
Pressure Plasma ◽  
Higher Power ◽  
Argon Plasma Jet

The aim of this study was to evaluate the effect of argon plasma jet, generated at atmospheric pressure, on Aspergillus sp. The molds were isolated from commercial white bread and inoculated on potato dextrose agar (PDA) containing 10% tartaric acid. The atmospheric pressure plasma jet (APPJ) was generated at a high frequency (43 kHz) power of 10, 20, or 30W and applied to the inoculated molds on PDA for 5, 10, or 20 minutes. The PDA plates were incubated for 3 and 6 days and the fungal inactivation was investigated directly under light microscope and indirectly by comparing the fungal dry weights. The results indicated that higher power supply and longer plasma exposure time led to more reduction in the fungal dry weight. The APPJ appears to be effective in destroying Aspergillus sp., yet the optimal plasma treatment condition remains to be optimized.

Ultrasonic Wave Detection in Atmospheric Pressure Plasma Using Fraunhofer Diffraction Effect

PIERS Online ◽  
2010 ◽  
Vol 6 (7) ◽  
pp. 636-639
Author(s):  
Toshiyuki Nakamiya ◽  
Fumiaki Mitsugi ◽  
Shota Suyama ◽  
Tomoaki Ikegami ◽  
Yoshito Sonoda ◽  
...  
Keyword(s):  
Ultrasonic Wave ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Diffraction Effect ◽  
Fraunhofer Diffraction ◽  
Wave Detection ◽  
Pressure Plasma
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