scholarly journals A Tailor-Made Protocol to Synthesize Yolk-Shell Graphene-Based Magnetic Nanoparticles for Nanomedicine

2018 ◽  
Vol 4 (4) ◽  
pp. 55 ◽  
Author(s):  
Raquel Rodrigues ◽  
Giovanni Baldi ◽  
Saer Doumett ◽  
Juan Gallo ◽  
Manuel Bañobre-López ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Drug Targeting ◽  
Colloidal Stability ◽  
Superparamagnetic Iron Oxide ◽  
In Vitro Tests ◽  
Chemotherapeutic Drug ◽  
On Demand ◽  
Superparamagnetic Behaviour ◽  
Ph Conditions

A simple tailor-made protocol to synthesize graphene-based magnetic nanoparticles (GbMNPs) for nanomedicine is herein reported. Different GbMNPs with very distinctive physicochemical and toxicological properties were synthesized by adjusting the number of carbon precursors in the coating of superparamagnetic iron oxide nanoparticles. In vitro tests show the ability to use these GbMNPs as intelligent and on-demand drug nanocarrier systems for drug delivery, exhibiting the following features: good colloidal stability, good loading capacity of the chemotherapeutic drug doxorubicin, high pH-controlled release of the encapsulated drug (targeting tumour acidic pH conditions), superparamagnetic behaviour and biocompatibility. Due to their combined properties (i.e., physicochemical, magnetic, and biocompatibility), GbMNPs show high potentiality to be combined with other biomedical techniques, such as magnetic hyperthermia, which can represent an enhancement in the treatment of cancer.

scholarly journals The Potential of Intrinsically Magnetic Mesenchymal Stem Cells for Tissue Engineering

2018 ◽  
Vol 19 (10) ◽  
pp. 3159 ◽  
Author(s):  
Fransiscus Kerans ◽  
Lisa Lungaro ◽  
Asim Azfer ◽  
Donald Salter
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Nanoparticles ◽  
Mammalian Cells ◽  
Cell Tracking ◽  
Magnetic Domain ◽  
Superparamagnetic Iron Oxide ◽  
In Vitro Assays

The magnetization of mesenchymal stem cells (MSC) has the potential to aid tissue engineering approaches by allowing tracking, targeting, and local retention of cells at the site of tissue damage. Commonly used methods for magnetizing cells include optimizing uptake and retention of superparamagnetic iron oxide nanoparticles (SPIONs). These appear to have minimal detrimental effects on the use of MSC function as assessed by in vitro assays. The cellular content of magnetic nanoparticles (MNPs) will, however, decrease with cell proliferation and the longer-term effects on MSC function are not entirely clear. An alternative approach to magnetizing MSCs involves genetic modification by transfection with one or more genes derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesizes single-magnetic domain crystals which are incorporated into magnetosomes. MSCs with either or mms6 and mmsF genes are followed by bio-assimilated synthesis of intracytoplasmic magnetic nanoparticles which can be imaged by magnetic resonance (MR) and which have no deleterious effects on MSC proliferation, migration, or differentiation. The stable transfection of magnetosome-associated genes in MSCs promotes assimilation of magnetic nanoparticle synthesis into mammalian cells with the potential to allow MR-based cell tracking and, through external or internal magnetic targeting approaches, enhanced site-specific retention of cells for tissue engineering.

Functionalization of γ-Fe2O3@SiO2 nanoparticles using the antiviral drug zidovudine: synthesis, characterization, in vitro cytotoxicity and DNA interaction studies

RSC Advances ◽  
2016 ◽  
Vol 6 (77) ◽  
pp. 73605-73616 ◽  
Author(s):  
Nahid Shahabadi ◽  
Monireh Falsafi ◽  
Foroozan Feizi ◽  
Reza Khodarahmi
Keyword(s):  
Magnetic Nanoparticles ◽  
Drug Targeting ◽  
Antiviral Drug ◽  
Sio2 Nanoparticles ◽  
Dna Interaction ◽  
In Vitro Cytotoxicity ◽  
Biological Applications ◽  
Interaction Studies

The aim of this study was to design and prepare γ-Fe2O3@SiO2-zidovudine magnetic nanoparticles (MNPs) for magnetic guided drug targeting and biological applications.

Magnetic nanoparticles for magnetic drug targeting

2015 ◽  
Vol 60 (5) ◽  
Author(s):  
Stefan Lyer ◽  
Raminder Singh ◽  
Rainer Tietze ◽  
Christoph Alexiou
Keyword(s):  
Magnetic Nanoparticles ◽  
Side Effects ◽  
Drug Targeting ◽  
Superparamagnetic Iron Oxide ◽  
Magnetic Drug Targeting ◽  
Oxide Nanoparticles ◽  
Treatment Concept ◽  
Substantial Progress ◽  
New Treatment ◽  
Tumor Area

AbstractNanomedicine and superparamagnetic iron oxide nanoparticles (SPIONs) are thought to have an important impact on medicine in the future. Especially in cancer therapy, SPIONs offer the opportunity of improving the effectivity of the treatment and reduce side effects by magnetic accumulation of SPION-bound chemotherapeutics in the tumor area. Although still some challenges have to be overcome, before the new treatment concept of magnetic drug targeting will reach the patients, substantial progress has been made, and promising results were shown in the last years.

Superparamagnetic Iron Oxide Nanoarticles for Biomedical Applications: a focus on PVA as a coating

2003 ◽  
Vol 789 ◽  
Author(s):  
M. Chastellain ◽  
A. Petri ◽  
H. Hofmann
Keyword(s):  
Iron Oxide ◽  
Biomedical Applications ◽  
Large Scale ◽  
Colloidal Stability ◽  
Superparamagnetic Iron Oxide ◽  
Narrow Size Distribution ◽  
Water Based ◽  
Co Precipitation

ABSTRACTNanoscaled particles showing a superparamagnetic behavior have been intensively studied these past years for biomedical applications and water-based ferrofluids turned out to be promising candidates for various in vivo as well as in vitro applications. Nevertheless, the lack of well-defined particles remains an important problem. One of the major challenges is still the large-scale synthesis of particles with a narrow size distribution. In this work iron oxide nanoparticles are obtained by classical co-precipitation in a water-based medium and are subsequently coated with polyvinyl alcohol. The thus obtained ferrofluids are studied and a focus is made on their colloidal stability.

The effect of grafting method on the colloidal stability and in vitro cytotoxicity of carboxymethyl dextran coated magnetic nanoparticles

2010 ◽  
Vol 20 (39) ◽  
pp. 8539 ◽  
Author(s):  
Mar Creixell ◽  
Adriana P. Herrera ◽  
Magda Latorre-Esteves ◽  
Vanessa Ayala ◽  
Madeline Torres-Lugo ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Colloidal Stability ◽  
In Vitro Cytotoxicity ◽  
Carboxymethyl Dextran

In vitro study of magnetic nanoparticles as the implant for implant assisted magnetic drug targeting

2011 ◽  
Vol 323 (14) ◽  
pp. 1903-1908 ◽  
Author(s):  
Jan O. Mangual ◽  
Misael O. Avilés ◽  
Armin D. Ebner ◽  
James A. Ritter
Keyword(s):  
Magnetic Nanoparticles ◽  
Drug Targeting ◽  
In Vitro Study ◽  
Vitro Study ◽  
Magnetic Drug Targeting

scholarly journals Chitosan-Functionalized Mg0.5Co0.5Fe2O4 Magnetic Nanoparticles Enhance Delivery of 5-Fluorouracil In Vitro

Coatings ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 446 ◽  
Author(s):  
Sanele Mngadi ◽  
Seipati Mokhosi ◽  
Moganavelli Singh ◽  
Wendy Mdlalose
Keyword(s):  
Magnetic Nanoparticles ◽  
Targeted Delivery ◽  
Colloidal Stability ◽  
Breast Adenocarcinoma ◽  
Transmission Electron ◽  
Physico Chemical ◽  
Anti Cancer ◽  
Diagnostic Agents ◽  
Diphenyl Tetrazolium Bromide

Magnetic nanoparticles (MNPs) have been widely investigated as a strategy to improve the delivery efficiency of therapeutic and diagnostic agents. Substituted iron oxides or ferrite nanoparticles (NPs) such as CoFe2O4 represent an interesting and novel class of MNPs, although they are under-researched in the field of biomedicine. In this study, chitosan-functionalized Mg0.5Co0.5Fe2O4 NPs were loaded with the anti-cancer 5-fluorouracil (5-FU) drug to yield CS-Mg0.5Co0.5Fe2O4-5FU. Transmission electron microscopy (TEM), Fourier Transform infra-red (FTIR) spectroscopy and nanoparticle tracking analysis (NTA) were employed to determine the physiochemical properties of the NPs. Physico-chemical characterizations confirmed spherical NPs with particle sizes of approximately 20.39 nm. Improved colloidal stability was observed, as determined by a zeta potential of approximately −20 mV for the drug-loaded CS-Mg0.5Co0.5Fe2O4 NPs. Drug encapsulation efficiencies of >60% were attained, showing a pH-dependent release of 5-FU. Cell viabilities investigated using the 3-[(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) and sulforodhamine B (SRB) assays in human embryonic kidney (HEK293), human breast adenocarcinoma (MCF-7) and human cervical cancer (HeLa) cells showed that these drug-loaded NPs exhibited more targeted tumor-specific cytotoxicities compared to free drugs. CS-Mg0.5Co0.5Fe2O4-5-FU NPs displayed significant targeted delivery potential to the investigated cancer cell lines. Conclusively, these results suggest that the CS-Mg0.5Co0.5Fe2O4-5-FU NPs are promising therapeutic delivery systems in anti-cancer treatment.

Microemulsions and nanoemulsions modified with cationic surfactants for improving the solubility and therapeutic efficacy of loaded drug indomethacin

2021 ◽  
Author(s):  
Alla B Mirgorodskaya ◽  
Marina Koroleva ◽  
Rushana A Kushnazarova ◽  
Ekaterina V Mishchenko ◽  
Konstantin A. Petrov ◽  
...  
Keyword(s):  
Cationic Surfactants ◽  
Colloidal Stability ◽  
Fluorescence Probe ◽  
Nile Red ◽  
Inflammatory Activity ◽  
In Vitro Tests ◽  
Prolonged Release ◽  
Anti Inflammatory

Abstract In this work, a noncovalent strategy was successfully used to modify colloidal stability and in vitro and in vivo efficacy of two amphiphilic formulations of the anti-inflammatory drug indomethacin. Namely, nanoemulsions and microemulsions based on oleic acid and nonionic surfactants have been produced and compared. The influence of cationic surfactants cetyltrimethylammonium bromide and its carbamate bearing analogue on the size characteristics, stability and ability to provide prolonged action of loaded drug indomethacin has been evaluated. Adding the positively charged molecules in the surface layer of nanoemulsions and microemulsions has shown the stability increase along with maintaining the size characteristics and homogeneity in time. Moreover, the carbamate modified analogue demonstrated beneficial behavior. Indomethacin loaded in microemulsions and nanoemulsions showed prolonged-release (10 to 15% release for 5 h) compared to a free drug (complete release for 5 h). The rate of release of indomethacin from nanoemulsions was slightly higher than from microemulsions and insignificantly decreased with an increase in the concentration of the cationic surfactant. For carbamate surfactant nanocarrier loaded with fluorescence probe Nile red, the ability to penetrate into the cell was supported by flow cytometry study and visualized by fluorescence microscopy. In vitro tests on anti-inflammatory activity of the systems demonstrated that the blood cell membrane stabilization increased in the case of modified microemulsion. The anti-inflammatory activity of the encapsulated drug was tested in rats using a carrageenan-induced edema model. Nanoemulsions without cationic surfactants appeared more efficient compared to microemulsions. Indomethacin emulsion formulations with carbamate surfactant added showed slower carrageenan-induced edema progression compared to unmodified compositions. Meanwhile, the edema completely disappeared upon treatment with emulsion loaded indomethacin after 4 h in the case of microemulsions versus 5 h in the case of nanoemulsions.

scholarly journals About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2733
Author(s):  
Alexis Loiseau ◽  
Julien Boudon ◽  
Céline Mirjolet ◽  
Véronique Morgand ◽  
Nadine Millot
Keyword(s):  
Prostate Cancer ◽  
Colloidal Stability ◽  
Metal Oxide Nanoparticles ◽  
Cancer Cell Line ◽  
Drug Efficacy ◽  
Prostate Cancer Cell Line ◽  
Resistance Mechanisms ◽  
Titanate Nanotubes ◽  
In Vitro Tests

The association between chemotherapeutic drugs and metal oxide nanoparticles has sparked a rapidly growing interest in cancer nanomedicine. The elaboration of new engineered docetaxel (DTX)‑nanocarriers based on titanate nanotubes (TiONts) was reported. The idea was to maintain the drug inside cancer cells and avoid multidrug resistance mechanisms, which often limit drug efficacy by decreasing their intracellular concentrations in tumor cells. HS‑PEGn‑COOH (PEG: polyethylene glycol, n = 3000, 5000, 10,000) was conjugated, in an organic medium by covalent linkages, on TiONts surface. This study aimed to investigate the influence of different PEG derivatives chain lengths on the TiONts colloidal stability, on the PEGn density and conformation, as well as on the DTX biological activity in a prostate cancer model (human PC‑3 prostate adenocarcinoma cells). In vitro tests highlighted significant cytotoxicities of the drug after loading DTX on PEGn‑modified TiONts (TiONts‑PEGn‑DTX). Higher grafting densities for shorter PEGylated chains were most favorable on DTX cytotoxicity by promoting both colloidal stability in biological media and cells internalization. This promising strategy involves a better understanding of nanohybrid engineering, particularly on the PEGylated chain length influence, and can thus become a potent tool in nanomedicine to fight against cancer.

scholarly journals Silica Coating of Ferromagnetic Iron Oxide Magnetic Nanoparticles Significantly Enhances Their Hyperthermia Performances for Efficiently Inducing Cancer Cells Death In Vitro

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2026
Author(s):  
Cristian Iacoviță ◽  
Ionel Fizeșan ◽  
Stefan Nitica ◽  
Adrian Florea ◽  
Lucian Barbu-Tudoran ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Cancer Cells ◽  
Colloidal Stability ◽  
Silica Coating ◽  
Neutral Red ◽  
Heating Performance ◽  
Iron Oxide Magnetic Nanoparticles ◽  
Ferromagnetic Iron

Increasing the biocompatibility, cellular uptake, and magnetic heating performance of ferromagnetic iron-oxide magnetic nanoparticles (F-MNPs) is clearly required to efficiently induce apoptosis of cancer cells by magnetic hyperthermia (MH). Thus, F-MNPs were coated with silica layers of different thicknesses via a reverse microemulsion method, and their morphological, structural, and magnetic properties were evaluated by multiple techniques. The presence of a SiO2 layer significantly increased the colloidal stability of F-MNPs, which also enhanced their heating performance in water with almost 1000 W/gFe as compared to bare F-MNPs. The silica-coated F-MNPs exhibited biocompatibility of up to 250 μg/cm2 as assessed by Alamar Blues and Neutral Red assays on two cancer cell lines and one normal cell line. The cancer cells were found to internalize a higher quantity of silica-coated F-MNPs, in large endosomes, dispersed in the cytoplasm or inside lysosomes, and hence were more sensitive to in vitro MH treatment compared to the normal ones. Cellular death of more than 50% of the malignant cells was reached starting at a dose of 31.25 μg/cm2 and an amplitude of alternating magnetic field of 30 kA/m at 355 kHz.

Sign in / Sign up

Export Citation Format

Share Document