scholarly journals Pre-clinical investigations of Verbena officinalis L. tisane effects against induced stress in Wistar male rats

2020 ◽  
Author(s):  
Sonia Marlene Rodrigues Oliveira ◽  
Elsa Dias ◽  
Maria de Lourdes Pereira
Keyword(s):  
Male Rats ◽  
Induced Stress ◽  
Clinical Investigations ◽  
Wistar Male Rats

scholarly journals Alpha2-Adrenoblockers Regulate Development of Oxidative Stress and Cognitive Behaviour of Rats under Chronic Acoustic Stress Conditions

2021 ◽  
Vol 14 (6) ◽  
pp. 529
Author(s):  
Magdalina Melkonyan ◽  
Ashkhen Manukyan ◽  
Lilit Hunanyan ◽  
Artem Grigoryan ◽  
Hayk Harutyunyan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Noise Exposure ◽  
Male Rats ◽  
Behavioral Activity ◽  
Induced Stress ◽  
Y Maze ◽  
Cognitive Behaviour ◽  
The Central Nervous System ◽  
High Level ◽  
Environment Noise

Noise is a wide-spread stress factor in modern life produced by urbanization, traffic, and an industrialized environment. Noise stress causes dysfunction and neurotransmission impairment in the central nervous system, as well as changes in hormone levels. In this study, we have examined the level of α-Tocopherol (α-T) and malondialdehyde (MDA) in plasma and the erythrocytes’ membrane (EM), as well as the behavioral characteristics of a noise-induced stress model in rats. In addition, the modulating effect of α2-adrenoblockers, beditin, and mesedin on the aforementioned parameters has been investigated. For these purposes, albino male rats were divided into four groups: (1) untreated; (2) noise-exposed, (3) noise-exposed and beditin-treated (2 mg/kg, i.p.), and (4) noise-exposed and mesedin-treated (10 mg/kg, i.p.) animals. Noise-exposed groups were treated with 91dBA noise on 60 days with a daily duration of 8 h. Increased MDA and decreased α-T levels in plasma and EM were observed upon chronic high-level noise exposure. Locomotor and behavioral activity assessed with a Y-maze revealed disorientation and increased anxiety under chronic noise exposure. Prominently, α2-adrenoblockers alleviated both behavioral deficits and oxidative stress, providing evidence for the involvement of α2-adrenoceptor in the pathophysiology of noise-induced stress.

Increased Urinary Excretion of Carnitine and Acylcarnitine by Mercuric Chloride Is Reversed by 2,3-Dimercapto-1-Propanesulfonic Acid in Rats

2010 ◽  
Vol 29 (3) ◽  
pp. 313-317
Author(s):  
Waleed A. Al-Madani ◽  
Nikhat J. Siddiqi ◽  
Abdullah S. Alhomida ◽  
Haseeb A. Khan ◽  
Ibrahim A. Arif ◽  
...  
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Mercuric Chloride ◽  
Free Carnitine ◽  
Male Rats ◽  
Significant Protection ◽  
Total Carnitine ◽  
Dose Dependent Increase ◽  
Dose Dependent ◽  
Wistar Male Rats

This investigation was aimed to study the effect of 2,3-dimercapto-1-propanesulfonic acid (DMPS) on mercuric chloride (HgCl2)-induced alterations in urinary excretion of various carnitine fractions including free carnitine (FC), acylcarnitine (AC), and total carnitine (TC). Different groups of Wistar male rats were treated with HgCl2 at the doses of 0.1, 0.5, 1.0, 2.0, and 3.0 mg/kg body weight, and the animals were sacrificed at 24 hours following HgCl2 injection. A separate batch of animals received HgCl2 (2 mg/kg) with or without DMPS (100 mg/kg) and sacrificed at 24 or 48 hours after dosing. Administration of HgCl2 resulted in statistically significant and dose-dependent increase in the urinary excretion of FC, AC, and TC in rats. However, the ratio of urinary AC:FC was significantly decreased by HgCl2. Pretreatment with DMPS offered statistically significant protection against HgCl2-induced alterations in various urinary carnitine fractions in rats.

scholarly journals UJI AKTIVITAS ANTIPIRETIK EKSTRAK ETANOL DAUN JARAK PAGAR (Jatropha curcas L.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus) GALUR WISTAR YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 342
Author(s):  
Angel T. Gosal ◽  
Edwin De Queljoe ◽  
Elly J. Suoth
Keyword(s):  
Vitamin C ◽  
Jatropha Curcas ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Male Rats ◽  
Anova Analysis ◽  
Jatropha Curcas L ◽  
Antipyretic Activity ◽  
Wistar Male Rats

ABSTRACTJatropha curcas L. leaves is plants containing saponins, flavonoid compounds such as kaempferol, nicotoflorin, quersitin, astragalin, ricinin and vitamin C. Flavonoids showed more than one hundred kinds of bioactivity, which is antipyretic, analgesic and anti-inflammatory effects. Several researches informed that flavonoid compound had antipyretic activity. The aim of this study was to examined the effect of ethanolic extract of Jatropha curcas L. leaves as antipyretic on white Wistar male rats (Rattus norvegicus) induced with DPT. There were 15 rats tested, divided into 5 groups that is negative control (CMC 1%), positive control (paracetamol) and treatment group of physic nut leaves extract 200 mg, 400 mg and 800 mg. Every rat was induced by fever using 0,4 ml of DPT vaccine intramuscularly. The temperature was measured before the rats were induced, 2-3 hour after induced and every 30 minute for 3 hours. The data obtained were analyzed by ANOVA (Analysis of Variants) and LSD. The results showed that the dose group of Jatropha curcas L. leaves ethanol extract had comparable activity with positive control of paracetamol in reducing fever. The conclusion was the ethanol extract of Jatropha curcas L. leaves has an antipyretic activity that could reduce temperature on white Wistar male rats at a dose of 200 mg, 400 mg and 800 mg. Keywords: Jatropha curcas L., antipyretic, Rattus norvegicus ABSTRAKDaun Jarak Pagar (Jatropha curcas L.) merupakan tanaman yang mengandung saponin, senyawa flavonoida antara lain kaempferol, nikotoflorin, kuersitin, astragalin, risinin dan vitamin C. Flavonoid menunjukkan lebih dari seratus macam bioaktivitas, antara lain efek antipiretik, analgetik dan antiinflamasi. Penelitian ini bertujuan untuk menguji aktivitas antipiretik ekstrak etanol daun jarak pagar (Jatropha curcas L.) terhadap tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Hewan uji yang digunakan sebanyak 15 ekor dan dibagi menjadi 5 kelompok yaitu kontrol negatif (CMC 1%), kontrol positif (parasetamol) dan kelompok dosis uji yaitu pemberian ekstrak daun jarak pagar 200 mg, 400 mg dan 800 mg. Setiap tikus diinduksi demam dengan vaksin DPT dosis 0,4 ml secara intramuskular. Pengukuran suhu dilakukan sebelum induksi, 2-3 jam setelah pemberian induksi dan setiap 30 menit selama 3 jam. Data yang diperoleh dianalisis dengan menggunakan uji ANOVA (Analysis of Variance) dan uji LSD (Least Significant Different). Hasil penelitian menunjukkan bahwa kelompok dosis ekstrak etanol daun jarak pagar memiliki aktivitas yang sebanding dengan kontrol positif parasetamol dalam menurunkan demam. Kesimpulannya ekstrak etanol daun jarak pagar (Jatropha curcas L.) memiliki aktivitas antipiretik yang dapat menurunkan suhu tubuh pada tikus putih jantan galur wistar (Rattus norvegicus) pada dosis 200 mg, 400 mg dan 800 mg. Kata kunci: Jatropha curcas L., antipiretik, Rattus norvegicus

scholarly journals Effects of Two Types of Restraint Stress on the Spontaneous Behaviour in Rats

2004 ◽  
Vol 47 (3) ◽  
pp. 177-180 ◽  
Author(s):  
Lenka Trnečková ◽  
Pavel Šída ◽  
Sixtus Hynie ◽  
Ivan Krejčí ◽  
Zdeněk Hliňák ◽  
...  
Keyword(s):  
Restraint Stress ◽  
Open Field Test ◽  
Exploratory Activity ◽  
Behavioural Response ◽  
Male Rats ◽  
Cognitive Responses ◽  
Activity Levels ◽  
Strong Suppression ◽  
Partial Immersion ◽  
Wistar Male Rats

Our previous findings suggested the existence of stressor-specific behavioural and cognitive responses in rats. In the present study, restraint stressor (immobilization, IMO) and restraint stressor combined with partial immersion of rats into water (IMO+C) were applied for 1 hour to Wistar male rats and their spontaneous behaviour was examined in the open field test. The classic behavioural parameters were recorded: crossing, rearing, and resting. When tested 1 and 4 hours after IMO+C, animals exhibited strong suppression of locomotor and exploratory activity (crossing and rearing); partial inhibition of both behavioural variables was found after IMO. Thus, substantial differences were observed in dependence on the length of period between the end of stressor application and the start of testing. In testing performed one week later, the locomotor and exploratory activity levels of both IMO and IMO+C animals corresponded to the control ones. These data suggest a differential behavioural response to both used stressors that may result from their different proportion of psychical and physical components. In conclusion, our results provide other data for the support of differential effects of two types of restraint stressors on spontaneous behaviour of animals exposed to a novel environment.

scholarly journals Nutritional Quality Evaluation of a Pea Protein Isolate in Rats with or Without Amino Acid Supplementation (P08-064-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Florence Guillin ◽  
Juliane Calvez ◽  
Laetitia Guérin-Deremaux ◽  
Catherine Lefranc-Millot ◽  
Nadezda Khodorova ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nitrogen Balance ◽  
Nutritional Quality ◽  
Protein Isolate ◽  
Male Rats ◽  
Amino Acid Supplementation ◽  
Pea Protein ◽  
Indispensable Amino Acid ◽  
Wistar Male Rats ◽  
Pea Protein Isolate

Abstract Objectives The global demand for protein is growing and it seems necessary to find new alternatives for animal proteins. Legumes are good candidates, as their indispensable amino acid (IAA) profile is relatively balanced. The aim of the study is to evaluate the nutritional quality of a pea protein isolate (NUTRALYS® pea protein), with or without methionine (Met) supplementation, using various indexes. We measured the Protein Efficiency Ratio (PER), the Digestible Indispensable Amino Acid Score (DIAAS) and the nitrogen balance in rats. Methods Study 1: 40 Wistar male rats weighing ∼50 g were fed ad libitum for 28 days with a diet containing 10% protein with variation in protein sources only. 5 groups (n = 8) were included: pea, casein, wheat gluten, pea-gluten combination, pea supplemented with Met. PER is obtained by the ratio between weight gain and protein intake throughout the experimental period. Study 2: 45 Wistar male rats weighing ∼250 g were housed in metabolic cages for 2 days and fed with a diet containing 14% protein of different sources. 5 groups (n = 9) were included: pea, casein, gluten, pea supplemented with Met, and protein-free diet for endogenous losses. Nitrogen content of diets, feces and urines was measured with an elementary analyzer for nitrogen balance (N ingested – N excreted). Then, rats were given a calibrated meal containing an indigestible marker and were euthanized 6 h later. Stomach, intestine, ileum, cecum, and colon contents were collected. DIAAS was calculated as follows: mg digestible IAA in 1 g of test protein*100/mg of IAA in 1 g of reference protein. IAA in ileum contents and diets were assayed by UPLC. Results Values are means ± SD. Met supplementation allows pea protein to reach the PER of casein, but association with gluten is not sufficient. Nitrogen balance values are higher for casein and pea + Met. Fecal digestibility of pea protein is higher than casein in our conditions. Analysis for DIAAS calculation are ongoing. Conclusions Pea protein is highly digestible in our conditions and Met supplementation can improve its capacity to insure growth. Funding Sources Roquette.

Sign in / Sign up

Export Citation Format

Share Document