scholarly journals Application of FTIR Spectroscopy for Quantitative Analysis of Blood Serum: A Preliminary Study

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2391
Author(s):  
Lyudmila V. Bel’skaya ◽  
Elena A. Sarf ◽  
Denis V. Solomatin
Keyword(s):  
Blood Serum ◽  
Ftir Spectroscopy ◽  
Ftir Spectra ◽  
Practical Implementation ◽  
Serum Samples ◽  
Absorption Bands ◽  
Reduced Gradient Method ◽  
Model Solutions ◽  
Freeze Dried ◽  
Generalized Reduced Gradient

The aim of this study was to analyze the possibility of simultaneous determination of the concentration of components from the characteristics of FTIR spectra using the example of a model blood serum. To prepare model solutions, a set of freeze-dried control sera based on bovine blood serum was used, certified for approximately 38 parameters. Based on the values of the absorbance and areas of absorption bands in the FTIR spectra of model solutions, a regression equation was constructed by solving a nonlinear problem using the generalized reduced gradient method. By using the absorbance of the absorption bands at 1717 and 3903 cm−1 and the areas of the absorption bands at 616, 3750, and 3903 cm−1, it is possible to simultaneously determine the concentrations of 38 components with an error of less than 0.1%. The results obtained confirm the potential clinical use of FTIR spectroscopy as a reagent-free express method for the analysis of blood serum. However, its practical implementation requires additional research, in particular, analysis of real blood serum samples and validation of the method.

Quantification of Sulphate in Ambient Aerosols by FTIR Spectroscopy

2013 ◽  
Vol 718-720 ◽  
pp. 1136-1139 ◽  
Author(s):  
Xiu Li Wei ◽  
Min Guang Gao ◽  
Jian Guo Liu ◽  
Liang Xu
Keyword(s):  
Air Quality ◽  
Ftir Spectroscopy ◽  
Ftir Spectra ◽  
Ir Absorption ◽  
Absorption Bands ◽  
Ambient Aerosols ◽  
Important Species ◽  
The Relationship

Aerosols are important species in the Earths atmosphere and influence both air quality and climate in many important. In this article the relationship between IR absorption bands and SO42- was discussed. Theν3 band of SO42- in FTIR Spectra was studied when used as Na2SO4 . The results indicated that the peak positions of SO42- were 984 cm-1 and 1133cm-1. And the relation between these peaks aera and the quality of SO42- was well linear. And we give the value of α in band of 900-1300 cm-1. we measured all of the samples on the Ge and not a point, so the quantitive was not been affected by the nonuniform sample on Ge.

scholarly journals Interrogation of IDH1 Status in Gliomas by Fourier Transform Infrared Spectroscopy

Cancers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3682
Author(s):  
James M. Cameron ◽  
Justin J. A. Conn ◽  
Christopher Rinaldi ◽  
Alexandra Sala ◽  
Paul M. Brennan ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Blood Serum ◽  
Ftir Spectroscopy ◽  
Rapid Determination ◽  
Fourier Transform Infrared ◽  
Attenuated Total Reflection ◽  
Serum Samples ◽  
Genetic Classification ◽  
Isocitrate Dehydrogenase 1 ◽  
Surgical Biopsy

Mutations in the isocitrate dehydrogenase 1 (IDH1) gene are found in a high proportion of diffuse gliomas. The presence of the IDH1 mutation is a valuable diagnostic, prognostic and predictive biomarker for the management of patients with glial tumours. Techniques involving vibrational spectroscopy, e.g., Fourier transform infrared (FTIR) spectroscopy, have previously demonstrated analytical capabilities for cancer detection, and have the potential to contribute to diagnostics. The implementation of FTIR microspectroscopy during surgical biopsy could present a fast, label-free method for molecular genetic classification. For example, the rapid determination of IDH1 status in a patient with a glioma diagnosis could inform intra-operative decision-making between alternative surgical strategies. In this study, we utilized synchrotron-based FTIR microanalysis to probe tissue microarray sections from 79 glioma patients, and distinguished the positive class (IDH1-mutated) from the IDH1-wildtype glioma, with a sensitivity and specificity of 82.4% and 83.4%, respectively. We also examined the ability of attenuated total reflection (ATR)-FTIR spectroscopy in detecting the biomolecular events and global epigenetic and metabolic changes associated with mutations in the IDH1 enzyme, in blood serum samples collected from an additional 72 brain tumour patients. Centrifugal filtration enhanced the diagnostic ability of the classification models, with balanced accuracies up to ~69%. Identification of the molecular status from blood serum prior to biopsy could further direct some patients to alternative treatment strategies.

scholarly journals Deposition of Zinc Oxide Coatings on Wood Surfaces Using the Solution Precursor Plasma Spraying Process

Coatings ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 183
Author(s):  
Ghiath Jnido ◽  
Gisela Ohms ◽  
Wolfgang Viöl
Keyword(s):  
Zinc Oxide ◽  
Ftir Spectroscopy ◽  
Photoelectron Spectroscopy ◽  
Color Change ◽  
Uv Light ◽  
Beech Wood ◽  
Nitrate Solution ◽  
Ftir Spectra ◽  
Solution Precursor ◽  
Wood Surfaces

In the present work, the solution precursor plasma spray (SPPS) process was used to deposit zinc oxide (ZnO) coatings on wood surfaces using zinc nitrate solution as precursor to improve the hydrophobicity and the color stability of European beech wood under exposure to ultraviolet (UV) light. The surface morphology and topography of the wood samples and the coatings were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The formation of ZnO was detected with the help of X-ray photoelectron spectroscopy (XPS) and by Fourier transform infrared (FTIR) spectroscopy. The FTIR spectra of the coated samples showed the typical Zn–O band at 445 cm−1. According to the XPS analysis, the coatings consist of two different Zn-containing species: ZnO and Zn(OH)2. Variation of the deposition parameters showed that the most significant parameters affecting the microstructure of the coating were the solution concentration, the deposition scan speed, and carrier gas flow rate. The wettability behaviors of the coated wood were evaluated by measuring the water contact angle (WCA). The coatings that completely covered the wood substrates showed hydrophobic behaviors. UV-protection of wood surfaces after an artificial UV light irradiation was evaluated by color measurements and FTIR spectroscopy. The ZnO-coated wood surfaces were more resistant to color change during UV radiation exposure. The total color change decreased up to 60%. Additionally, the FTIR spectra showed that the wood surfaces coated with ZnO had more stability. The carbonyl groups formation and C=C-bonds consumption were significantly lower.

scholarly journals Effect of Water Leaching on Photodegraded Scots Pine and Spruce Timbers Monitored by FTIR Spectroscopy

Forests ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 833
Author(s):  
Edina Preklet ◽  
László Tolvaj ◽  
Eszter Visi-Rajczi ◽  
Tamás Hofmann
Keyword(s):  
Ftir Spectroscopy ◽  
Scots Pine ◽  
Uv Irradiation ◽  
Chemical Elements ◽  
Uv Light ◽  
Carbonyl Groups ◽  
Water Leaching ◽  
Absorption Bands ◽  
Chemical Changes ◽  
Absorption Increase

The goal of this research was the systematic study and comparison of the divided individual effects of UV light irradiation and water leaching during artificial weathering. Spruce (Picea abies Karst.) and Scots pine (Pinus sylvestris L.) samples were irradiated by ultraviolet (UV) light. Another sequence of samples was treated with the combination of UV irradiation and water leaching. The total extent of UV treatment was 20 days for both series of samples. Time relation of UV irradiation and water leaching was 2:1. The chemical changes were observed by FTIR spectroscopy. The difference spectrum was used for determination of the chemical changes. Degradation of lignin was greater for the leached samples than for the pure UV treated samples. Scots pine suffered greater lignin degradation than spruce, and produced higher absorption increase on the absorption region of unconjugated carbonyls. The unconjugated carbonyl groups were the most responsive chemical elements to leaching. Spruce was more susceptible to leaching of unconjugated carbonyl groups than Scots pine. Two absorption bands of unconjugated carbonyl groups at 1706 and 1764 cm−1 wavenumbers were produced by photodegradation. The absorption band at 1764 cm−1 was more sensitive to water leaching than the band at 1706 cm−1.

scholarly journals Characterization of essential oils extracted from different aromatic plants by FTIR spectroscopy

2017 ◽  
Vol 74 (1) ◽  
pp. 37 ◽  
Author(s):  
Maria Ioana MORAR ◽  
Florinela FETEA ◽  
Ancuta Mihaela ROTAR ◽  
Melinda NAGY ◽  
Cristina Anamaria SEMENIUC
Keyword(s):  
Essential Oils ◽  
Ftir Spectroscopy ◽  
Ftir Spectra ◽  
Aromatic Plants

Four essential oils isolated from dried leaves of parsley, lovage, basil, and thyme were investigated by FTIR spectroscopy. FTIR spectra revealed characteristic key bands for the main compounds of tested essential oils.

Determination of urea in 10-?l blood serum samples with a urease reactor / ion-selective electrode cell

1983 ◽  
Vol 81 (1-2) ◽  
pp. 135-147 ◽  
Author(s):  
U. Thanei-Wyss ◽  
W. E. Morf ◽  
P. Lienemann ◽  
Z. Stefanac ◽  
I. Mostert ◽  
...  
Keyword(s):  
Blood Serum ◽  
Ion Selective Electrode ◽  
Selective Electrode ◽  
Serum Samples ◽  
Electrode Cell

Analysis of candlenut oil as oil adulterant in three functional oils of soybean oil, sunflower oil and grapeseed oil in quaternary mixture systems using FTIR spectroscopy and chemometrics

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 248-253
Author(s):  
A.B. Riyanta ◽  
S. Riyanto ◽  
E. Lukitaningsih ◽  
A. Rohman
Keyword(s):  
Root Mean Square Error ◽  
Soybean Oil ◽  
Ftir Spectroscopy ◽  
Mean Square Error ◽  
Root Mean Square ◽  
Sunflower Oil ◽  
Ftir Spectra ◽  
Mean Square ◽  
Grapeseed Oil ◽  
Quaternary Mixture

Soybean oil (SBO), sunflower oil (SFO) and grapeseed oil (GPO) contain high levels of unsaturated fats that are good for health and have proximity to candlenut oil. Candlenut oil (CNO) has a lower price and easier to get oil from that seeds than other seed oils, so it is used as adulteration for gains. Therefore, authentication is required to ensure the purity of oils by proper analysis. This research was aimed to highlight the FTIR spectroscopy application with multivariate calibration is a potential analysis for scanning the quaternary mixture of CNO, SBO, SFO and GPO. CNO quantification was performed using multivariate calibrations of principle component (PCR) regression and partial least (PLS) square to predict the model from the optimization FTIR spectra regions. The highest R2 and the lowest values of root mean square error of calibration (RMSEC) and root mean square error of prediction (RMSEP) were used as the basis for selection of multivariate calibrations created using several wavenumbers region of FTIR spectra. Wavenumbers regions of 4000-650 cm-1 from the second derivative FTIR-ATR spectra using PLS was used for quantitative analysis of CNO in quaternary mixture with SBO, SFO and GPO with R2 calibration = 0.9942 and 0.0239% for RMSEC value and 0.0495%. So, it can be concluded the use of FTIR spectra combination with PLS is accurate to detect quaternary mixtures of CNO, SBO, SFO and GPO with the highest R2 values and the lowest RMSEC and RMSEP values.

Synthesis of Galactomannan Sulfate-Citrate

2022 ◽  
Vol 1049 ◽  
pp. 218-223
Author(s):  
Aleksandr S. Kazachenko ◽  
Yuriy N. Malyar ◽  
Anna S. Kazachenko
Keyword(s):  
Biological Activity ◽  
Ftir Spectroscopy ◽  
X Ray Diffraction ◽  
Absorption Bands ◽  
X Ray ◽  
Mixed Ester ◽  
First Time ◽  
Derivatives Of

Sulfated derivatives of polysaccharides have anticoagulant, hypolipedimic and other biological activity. In this work, a complex mixed ester of galactomannan, its sulfate-citrate, was obtained for the first time. The introduction of citrate and sulfate groups was proved by FTIR spectroscopy by the appearance of corresponding absorption bands. It was shown by X-ray diffraction that the introduction of the citrate group leads to the amorphization of the galactomannan structure.

Correlated vibrations of the tetrahedral and octahedral complexes and splitting of the absorption bands in FTIR spectra of Li-Zn ferrites

2017 ◽  
Vol 92 ◽  
pp. 267-272 ◽  
Author(s):  
V. Rathod ◽  
A.V. Anupama ◽  
R. Vijaya Kumar ◽  
V.M. Jali ◽  
B. Sahoo
Keyword(s):  
Ftir Spectra ◽  
Absorption Bands
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