scholarly journals Survey, Identification, and Pathogenicity of Ceratocystis fimbriata Complex Associated with Wilt Disease on Acacia mangium in Malaysia

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1782
Author(s):  
Samsuddin Ahmad Syazwan ◽  
Ahmad Mohd-Farid ◽  
Wan-Azhar Wan-Muhd-Azrul ◽  
Hishamuddin Muhammad Syahmi ◽  
Abdullah Mohd Zaki ◽  
...  

Ceratocystis wilt disease surveys were conducted in three selected Malaysian Acacia mangium plantations. These completed surveys revealed the occurrence of the wilt disease, with the incidence of infection ranging from 7.5% to 13.6%. Signs of wood-boring insects, bark peeling due to squirrel activity, and pruning wounds were often associated with this disease. The fungus most frequently isolated from the diseased trees was the Ceratocystis fungus. The analysis on the morphological characteristics has identified the fungus as Ceratocystis fimbriata complex. Phylogenetic analysis based on the sequences of the ITS, and concatenated sequences of EF1α-βT regions grouped the isolates within the C. fimbriata sensu stricto, in comparison to other C. fimbriata isolates. Pathogenicity tests were conducted on six to nine-month-old healthy A. mangium seedlings by inoculating these seedlings with eight out of the 16 isolates. The results demonstrated that all the isolates were pathogenic, with mortality beginning as early as two weeks after inoculation. However, an ANOVA test indicated a significant difference between the pathogenicity levels among the fungal isolates. The results also showed that pathogen aggressiveness was not correlated with geographical origin. A host range test was also conducted by using C. fimbriata SSB3 and FRIM1162 isolates against several forest plantation species. The findings suggested that only A. mangium was susceptible to C. fimbriata. The other species remained healthy with no symptoms of infection even after seven weeks of treatment, as compared to the A. mangium species, where between 38 to 60% of the inoculated plants had died. This study provides new information on the status of Ceratocystis wilt disease, especially on the occurrence and effects on A. mangium plantation, by giving insights on how to control and manage this ferocious plant pathogen in the future.

2017 ◽  
Vol 5 (1) ◽  
pp. 15-22
Author(s):  
Mohammad Nizam Uddin ◽  
Barua Subhasis ◽  
Boonyanuphap Jaruntorn

This study was aimed to identify the dominant species establishing during regeneration, their species diversity and whether selection thinning could enhance the regeneration of secondary forest species. Chittagong University Forest was selected for the study and harvested and unharvested sites of forest plantations were demarcated and set 30 quadratic plots (3m × 3m) for each site. Ten per cent selection thinning was done in harvested site although a little anthropogenic disturbance was noticed in both the plantations.  Regenerations were identified into species level and their numbers were counted. Shannon-Weiner diversity index for unharvested plantation was 2.21 and it was 1.45 for harvested plantation. Species evenness index was recorded as 0.81 and 0.74 for unharvested and harvested plantations respectively. Both the plantations did not show a significant difference in diversity of species. According to the Important Value Index (IVI) the dominant species in the harvested site were Acacia auriculiformis followed by Acacia mangium and Albizia lebbeck. In the unharvested site, IVI was the highest for Acacia auriculiformis followed by Aphanamixis polystachya and Lagerstroemia speciosa. In both the sites, Acacia auriculiformis had a significantly (p<0.05) higher number of regeneration in harvested plantations than those of unharvested plantations. Therefore, thinning has a positive impact on natural regeneration and Acacia auriculiformis is the best for natural regeneration in plantations.


1984 ◽  
Vol 35 (4) ◽  
pp. 427 ◽  
Author(s):  
SJ Rowland

The status of bream, from estuaries near Narooma on the south coast of New South Wales, which display morphological characteristics intermediate to those distinguishing the species A. australis and A. butcheri was investigated. The two species were separated electrophoretically at a malate dehydrogenase locus (Mdh-1) where they are fixed for alternative alleles, and by a significant difference in allele frequency at the lactate dehydrogenase locus Ldh-A. Because four individual bream, which displayed intermediate morphometric characteristics, have inherited both species-specific Mdh-1 alleles and are heterozygous at the Ldh-A locus, their status as hybrids is determined. The presence of three bream with allelic characteristics of A. australis at the Mdh-1 locus but allelic characteristics of A. butcheri at the Ldh-A locus is evidence for the presence of later generation or backcross hybrids. No evidence of introgression was found. The reproduction of A. australis and A. butcheri is temporally and spatially isolated under normal circumstances but in some landlocked, coastal lakes there is a breakdown of these isolating mechanisms. Because A. australis and A. butcheri differ morphometrically and ecologically over their sampled ranges, and only appear to hybridize in the unusual environmental conditions of closed lakes, it is suggested that their specific status be retained. Using electrophoretic data, a high level of genic similarity (I = 0.91) was found to exist between the two species. This, together with the close relationship indicated by the production of fertile F1 hybrids, suggests a recent speciation.


2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e17003-e17003
Author(s):  
Tatiana A. Zykova ◽  
Alexey N. Shevchenko ◽  
Irina A. Khomutenko ◽  
Elena V. Filatova ◽  
Elena A. Shevyakova ◽  
...  

e17003 Background: The etiological role of human papillomavirus (HPV) in the development of squamous cell carcinoma of the cervix and larynx is considered proven. At the same time, there are conflicting data in the literature on the role of HPV in the development of bladder cancer (BC). The aim of the present study was to evaluate the prevalence of HPV infection in patients with BC, identifying the relationship between the status of infection and the morphological characteristics of the tumor. Methods: The study included 46 patients (10 women and 36 men) with morphologically confirmed urothelial cancer. Samples of urine, tumor tissue and a healthy bladder mucosa were examined. HPV DNA was determined by real-time PCR. Results: 28 (60.9%) patients were infected with high-risk HPV, including 6 (60.0%) women and 22 (61.1%) men. In 14 (30.4%) patients, HPV DNA was found in the tissue of the bladder mucosa: in 6 (13.0%) cases it was only in tumor tissue, in 4 (8.7%) only in healthy mucosa, in 4 (8.7%) simultaneously in healthy and tumor tissue. HPV DNA was detected in urine in 22 (47.8%) patients. The simultaneous presence of HPV DNA in the tissue of the bladder mucosa and urine was found in 8 (28.6%) HPV-positive patients. Single HPV infection was found in 20 (71.4%) patients, two types were found in 8 (28.6%). When analyzing type diversity, it was found that HPV16 infected 14 (38.9%) patients; HPV18, 31, 39 and 56 were determined in 4 (11.1%) cases each; 33, 52 and 59 types in 2 (5.6%) cases. Chronic inflammation was noted in 12 (26.1%) patients, 6 (50.0%) of them were HPV-positive. Among HPV-positive patients, invasive cancer was registered in 18 (64.3%), non-invasive cancer in 10 (35.7%) patients. Among HPV-negative patients, these rates were 14 (77.8%) and 4 (22.2%), respectively (p > 0.05). In the group of HPV-positive patients, tumors with a low degree of differentiation were found in 16 (57.1%), moderate in 6 (21.4%), high in 6 (21.4%). Among HPV-negative patients, these indicators were 4 (22.2%), 12 (66.7%) and 2 (11.1%), respectively (p > 0.05). Conclusions: HPV infection was detected in 60.9% of patients, including tumor tissue infection in 21.7% of patients. The most common HPV type was HPV16. There was no statistically significant difference between HPV-positive and negative tumors.


Author(s):  
Aliza Sultana ◽  
Ashok Kumar Paul ◽  
Meher Un Nessa

This study was conducted in the Khulna metropolitan city to determine the status of noise pollution at major traffic intersections and assess the possible effect of noise on regular noise afflicted people. For this research, five busiest roadways and traffic intersections; two mixed zone and three commercial zones were selected purposively and 'ST-8850 Sound Level Meter' was used to record the noise pressure level. Average noise pressure level and some noise parameters (Leq, L10, L50, L90, and Lnp) were measured at different shifts in each location both on working days and holidays. The result evidently specified that noise level in all major traffic circles of Khulna metropolitan city was much higher than the recommended threshold of acceptable noise level set by the Department of Environment (DOE) of Bangladesh. It was observed that the mixed area (Sonadanga, Gollamari) tends to have a higher average noise level than those of the commercial area (Shibbari, Notunrasta, Dakbangla). The noise pollution level (Lnp) in mixed areas varied from 92.77 dB (A) to 104.74 dB(A) on working days and 86.9 dB(A) to 105.5 dB(A) on holidays. On working days, the highest Leq was observed 92.65 ± 4.15 dB(A) in Sonadanga (mixed area), whereas the lowest was 85.13 ± 2.83 dB(A) in Dakbangla (commercial area). However, on holidays the highest level of Leq was found 90.41 ± 2.25 dB(A) in Sonadanga, while the lowest levels were 80.43 ± 3.20 dB(A) in Dakbangla. One-way ANOVA with LSD post-hoc test results illustrated that on working days there was a significant difference between the noise pollution levels (NPL) in different shifts of day time (F = 8.412, p < .05). The respondents of the study area addressed that they were the most affected by annoyance (70%), headache (62%), and hearing loss (46%) due to regular exposure to the traffic noise. Therefore, it is recommended to enhance awareness regarding the cause and effect of noise pollution among the noise producers and oppressed people. Subsequently, administrative measures have to take to attenuate noise pollution and indemnify the quality health of the citizens.


Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 290
Author(s):  
Giacomo Cremonesi ◽  
Francesco Bisi ◽  
Lorenzo Gaffi ◽  
Thet Zaw ◽  
Hla Naing ◽  
...  

The effects of human disturbance represent one of the major threats for wildlife conservation. Many studies have shown that wildlife avoids or reduces direct contact with human activities through changes in activity patterns, and by minimizing spatiotemporal overlap. In this study, we investigated the possible effects of human presence on the temporal activity of medium-to-large mammals using two areas in Myanmar that differ in the intensity of human disturbance. We monitored temporal segregation mechanisms using camera trapping data and with two statistical approaches: daily activity overlaps between humans and wildlife and circular statistics. We did not find a significant difference in overlapping activity between areas but, thanks to circular statistics, we found that some species show changes in activity patterns, suggesting temporal avoidance. We observed that the daily activity of five species differed between areas of Myanmar, likely adopting mechanisms to reduce overlap in areas highly frequented by humans. Interestingly, these species are all threatened by hunting or poaching activities, four of which have been described in literature as “cathemeral”, or species that are active through day and night. This study suggests that some species adapt their behavior, at least partially, to avoid human presence in habitats with higher anthropic occurrence and increase our knowledge on the status of medium–large mammals in a poorly studied country as Myanmar.


2018 ◽  
Vol 71 (1) ◽  
pp. 135-142 ◽  
Author(s):  
P. K. Shukla ◽  
Savita Varma ◽  
Tahseen Fatima ◽  
Anju Bajpai ◽  
Rupesh Mishra ◽  
...  

2020 ◽  
Vol 32 (1) ◽  
pp. 23-28
Author(s):  
TOFAEL AHMED SUMON ◽  
MD. ASHRAF HUSSAIN ◽  
FARJANA AKHTER MITA ◽  
JOYANTA BIR ◽  
SARKER MOHAMMED IBRAHIM KHALIL

The study revealed the status of formalin used in four commercially important fishes namely Rohu (Labeo rohita), Catla (Catla catla), Mrigal (Cirrhinus cirrhosus) and Hilsa (Tenualosa ilisha) from Sylhet and Moulvibazar district for a period of four months from April to July 2016. A total of 100 fish samples from different town and village markets were collected and analyzed instantly on the market to detect the presence of formalin by using a kit developed by Bangladesh Council of Scientific and Industrial Research (BCSIR). Among the analyzed fishes 46.7, 26.7, 20.0 and 13.3% of Rohu, Catla, Mrigal and Hilsa, respectively were found treated with formalin from Sylhet district. Similarly, formalin was also detected in 40, 20, 20 and 20% of investigated Rohu, Catla, Mrigal and Hilsa from Moulvibazar district, individually. There was no significant difference in formalin used between town and village market, whereas a significant difference in application of formalin in domestic and imported fishes except Catla has been identified. Two-third of imported Rohu (66.7%) was found formalin positive followed by Hilsa (44.4%), Mrigal (40%) and Catla (35.7%). Amongst the domestic fishes, presence of formalin was detected in 27.8, 18.8, 10.0 and 4.8% in Rohu, Catla, Mrigal and Hilsa, separately. This devastating use of formalin in food fishes should be banned through proper enactment of the existing laws of the country, as well as timely and appropriate implementation of government monitoring is mandatory to combat the unethical formalin use.


2021 ◽  
Vol 9 (47) ◽  
pp. 11515-11527
Author(s):  
Shubhashree Dash

COVID-19 pandemic has influenced every aspect of human life including Education and Mental Health of the learners. Thus, the present study was aimed at assessing the status of online learning and mental health of online learners and to investigate the relationship between the two variables. The descriptive survey method was adopted for the present study and the collected data analysed quantitatively. For collection of the data two self-made tools were used namely Status of Online Learning Scale for Learners and Mental Health Assessment Scale for Learners. The tools were administered on 140 students. The tabulated data was analysed through the Mean, Standard Deviation, correlation and t test. The results revealed that learners are moderately active in online learning. Mental health of the online learners is moderately stable. Online learning is negatively affecting the mental health of the online learners and there was no significant difference found between the mental health of online learners in terms of the gender and the locality.


2021 ◽  
Vol 104 (3) ◽  
pp. 003685042110429
Author(s):  
Abdulaziz M Albargi ◽  
Ali A Assiry ◽  
Hammam A Bahammam ◽  
Mohand Y Alassiri ◽  
Anand Marya ◽  
...  

Objectives: This study aimed to evaluate the truthfulness of patients about their pre-appointment COVID-19 screening tests at a dental clinic. Methods: A total of 613 patients were recruited for the study from the dental clinic at the Faculty of Dentistry, Najran University, Saudi Arabia. The data collection was done in three parts from the patients who visited the hospital to receive dental treatment. The first part included the socio-demographic characteristics of the patients and the COVID-19 swab tests performed within the past 14 days. The second part was the clinical examination, and the third part was a confirmation of the swab test taken by the patient by checking the Hesen website using the patient ID. After data collection, statistical analysis was carried out using SPSS 26.0. Descriptive analysis was done and expressed as mean, standard deviation, frequency, and percentage (%). A cross-tabulation, also described as a contingency table, was used to identify trends and patterns across data and explain the correlation between different variables. Results: It was seen from the status of the swab test within 14 days of the patient's arrival at the hospital for the dental treatment that 18 (2.9%) patients lied about the pre-treatment swab test within 14 days, and 595 (97.1%) were truthful. The observed and expected counts showed across genders and diagnosis a statistically significant difference ( p < 0.001), and there was no significant difference seen across different age groups ( p = 0.064) of the patients. Conclusions: Dental healthcare workers are worried and assume a high risk of COVID-19 infection as the patients are not truthful about the pre-treatment COVID-19 swab test. Routine rapid tests on patients and the healthcare staff are a feasible option for lowering overall risks.


10.2196/14826 ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. e14826 ◽  
Author(s):  
Fuzhi Wang ◽  
Zhuoxin Wang ◽  
Weiwei Sun ◽  
Xiumu Yang ◽  
Zhiwei Bian ◽  
...  

Background As representatives of health information communication platforms accessed through mobile phones and mobile terminals, health-related WeChat public accounts (HWPAs) have a large consumer base in the Chinese-speaking world. However, there is still a lack of general understanding of the status quo of HWPAs and the quality of the articles they release. Objective The aims of this study were to assess the conformity of HWPAs to the Health on the Net Foundation Code of Conduct (HONcode) and to evaluate the suitability of articles disseminated by HWPAs. Methods The survey was conducted from April 23 to May 5, 2019. Based on the monthly (March 1-31, 2019) WeChat Index provided by Qingbo Big Data, the top 100 HWPAs were examined to evaluate their HONcode compliance. The first four articles published by each HWPA on the survey dates were selected as samples to evaluate their suitability. All materials were assessed by three raters. The materials were assessed using the HONcode checklist and the Suitability Assessment of Materials (SAM) score sheet. Data analysis was performed with SPSS version 17.0 (SPSS Inc, Chicago, IL, USA) and Excel version 2013 (Microsoft Inc, Washington DC, USA). Results A total of 93 HWPAs and 210 of their released articles were included in this study. For six of the eight principles, the 93 HWPAs nearly consistently did not meet the requirements of the HONcode. The HWPAs certified by Tencent Corporation (66/93, 71%) were generally slightly superior to those without such certification (27/93, 29%) in terms of compliance with HONcode principles. The mean SAM score for the 210 articles was 67.72 (SD 10.930), which indicated “adequate” suitability. There was no significant difference between the SAM scores of the articles published by certified and uncertified HWPAs (P=.07), except in the literacy requirements dimension (tdf=97=–2.418, P=.02). Conclusions The HWPAs had low HONcode conformity. Although the suitability of health information released by HWPAs was at a moderate level, there were still problems identified, such as difficulty in tracing information sources, excessive implicit advertisements, and irregular usage of charts. In addition, the low approval requirements of HWPAs were not conducive to improvement of their service quality.


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