Studying the interrelation between the HPV infection status and the morphological features of bladder cancer.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e17003-e17003
Author(s):  
Tatiana A. Zykova ◽  
Alexey N. Shevchenko ◽  
Irina A. Khomutenko ◽  
Elena V. Filatova ◽  
Elena A. Shevyakova ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Tumor Tissue ◽  
Morphological Characteristics ◽  
Hpv Infection ◽  
Invasive Cancer ◽  
Bladder Mucosa ◽  
Hpv Dna ◽  
Significant Difference ◽  
The Status

e17003 Background: The etiological role of human papillomavirus (HPV) in the development of squamous cell carcinoma of the cervix and larynx is considered proven. At the same time, there are conflicting data in the literature on the role of HPV in the development of bladder cancer (BC). The aim of the present study was to evaluate the prevalence of HPV infection in patients with BC, identifying the relationship between the status of infection and the morphological characteristics of the tumor. Methods: The study included 46 patients (10 women and 36 men) with morphologically confirmed urothelial cancer. Samples of urine, tumor tissue and a healthy bladder mucosa were examined. HPV DNA was determined by real-time PCR. Results: 28 (60.9%) patients were infected with high-risk HPV, including 6 (60.0%) women and 22 (61.1%) men. In 14 (30.4%) patients, HPV DNA was found in the tissue of the bladder mucosa: in 6 (13.0%) cases it was only in tumor tissue, in 4 (8.7%) only in healthy mucosa, in 4 (8.7%) simultaneously in healthy and tumor tissue. HPV DNA was detected in urine in 22 (47.8%) patients. The simultaneous presence of HPV DNA in the tissue of the bladder mucosa and urine was found in 8 (28.6%) HPV-positive patients. Single HPV infection was found in 20 (71.4%) patients, two types were found in 8 (28.6%). When analyzing type diversity, it was found that HPV16 infected 14 (38.9%) patients; HPV18, 31, 39 and 56 were determined in 4 (11.1%) cases each; 33, 52 and 59 types in 2 (5.6%) cases. Chronic inflammation was noted in 12 (26.1%) patients, 6 (50.0%) of them were HPV-positive. Among HPV-positive patients, invasive cancer was registered in 18 (64.3%), non-invasive cancer in 10 (35.7%) patients. Among HPV-negative patients, these rates were 14 (77.8%) and 4 (22.2%), respectively (p > 0.05). In the group of HPV-positive patients, tumors with a low degree of differentiation were found in 16 (57.1%), moderate in 6 (21.4%), high in 6 (21.4%). Among HPV-negative patients, these indicators were 4 (22.2%), 12 (66.7%) and 2 (11.1%), respectively (p > 0.05). Conclusions: HPV infection was detected in 60.9% of patients, including tumor tissue infection in 21.7% of patients. The most common HPV type was HPV16. There was no statistically significant difference between HPV-positive and negative tumors.

scholarly journals Italian observational study on HPV infection, E6, and p16 expression in men with penile cancer

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Narcisa Muresu ◽  
Giovanni Sotgiu ◽  
Laura Saderi ◽  
Illari Sechi ◽  
Antonio Cossu ◽  
...  
Keyword(s):  
Penile Cancer ◽  
Tertiary Care ◽  
Hpv Infection ◽  
Molecular Techniques ◽  
Care Hospital ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Diagnosis And Prognosis ◽  
Significant Difference

Abstract Background Human Papillomavirus (HPV) infection is one of the most important causes of cancer. It can play a role in cervical and extra-cervical cancers. Penile cancer is rare, even if an increasing trend was recently reported. Aim of the present study was to assess the prevalence and distribution of HPV genotypes in cases of penile cancer diagnosed in Sardinia, Italy. Surrogate markers of HPV infection (i.e., E6 and p16 genes) were also evaluated in all cases. Methods An observational, retrospective study which recruited all cases of penile cancer diagnosed between 2002 and 2019 at a tertiary care hospital in Sardinia, Italy, was carried out. HPV-DNA detection and genotyping were performed by Real-time PCR. Specimens were tested for oncogene E6 mRNA and for p16(INK4a) expression. Results HPV prevalence was 28.1% (9/32); HPV-16 was the most prevalent genotype (7/9, 77.8%). p16INK4a positivity was found in 66.7% of the samples with a statistically significant difference between HPV-positive and -negative groups. E6-transcript was detected in 71% of the HPV-16 positive samples. The overall survival was not statistically different between HPV-positives and -negatives. Discussion The present study confirms the etiologic role of HPV in penile cancer and supports the adoption of vaccination strategies in men and women. Further studies should clarify the diagnostic and prognostic role of E6 and p16 proteins. Conclusion HPV infection can favor the occurrence of penile cancer, whose diagnosis and prognosis could be improved with the implementation of validated molecular techniques.

Prevalence of HPV 16 and HPV 18 in cervical cancer in Minia Governorate

2020 ◽  
Vol EJMM29 (4) ◽  
pp. 57-63
Author(s):  
Mohammed S. Mohammed ◽  
Ahmed S. Sand ◽  
Noha A. Hassuna
Keyword(s):  
Cervical Cancer ◽  
Real Time ◽  
Real Time Pcr ◽  
Critical Role ◽  
Hpv Infection ◽  
Hpv Dna ◽  
Contraceptive Usage ◽  
Significant Difference ◽  
Histopathological Results

Background: Human Papilloma virus (HPV) have a critical role in cervical cancer especially type 16 and 18 and great efforts were done to determine the role of HPV in cervical cancer. Objective: The objective of this work is evaluation of the prevalence of cervical cancer and determination of the role of HPV in cervical cancer, in Minia Governorate. Methodology: Colposcopic-directed biopsy was done for one hundred females by colposcopist. One hundred women were included in this study for detection of HPV infection by histopathology, real time PCR and sequencing. One hundred biopsies were subjected to analysis for detection of HPV types 16 and 18 by histopathology, real time PCR and sequencing. Results and conclusion: Our histopathological results revealed that 15% of samples were positive for HPV and by HPV DNA real time PCR, 14% were positive. Our study reported that the infection was single in 9% and mixed in 5% and HPV type 16 was higher than type 18. The age range across the sample was 18–55 years with a median age of 35 years. The women patients with subtype 18 had the highest median age. Contraceptive usage had a statistically significant difference especially using OC.

scholarly journals Lack of HPV in pterygium with no evidence of autoinoculation and the role of cytokines in pterygium with dry eye

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lita Uthaithammarat ◽  
Ngamjit Kasetsuwan ◽  
Yuda Chongpison ◽  
Pimpetch Kasetsuwan ◽  
Usanee Reinprayoon ◽  
...  
Keyword(s):  
Dry Eye ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Urine Samples ◽  
Vascular Endothelial ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Polymerase Chain ◽  
Endothelial Growth Factor

AbstractThis study evaluated human papillomavirus’s (HPV) role in pterygium pathogenesis, its autoinoculation from genitalia to ocular surface, potential cytokines involved, and crosstalk cytokines between pterygium and dry eye (DE). This cross-sectional study enrolled 25 healthy controls (HCs) and 116 pterygium patients. Four subgroups of pterygium and DE were used in cytokine evaluations. Conjunctival and pterygium swabs and first-void urine samples (i.e., genitalia samples) were collected for HPV DNA detection using real-time polymerase chain reaction. Tear cytokines interleukin (IL)-6, IL-18, and vascular endothelial growth factor (VEGF) in tears were evaluated. No HPV DNA was detected in conjunctival or pterygium swabs. No association was found between HPV DNA in urine samples and that from conjunctival or pterygium swabs. Tear VEGF levels were significantly higher in pterygium patients than in HCs, with no markedly different levels between primary and recurrent pterygia. Tear IL-6, IL-18, and tear VEGF were significantly higher in participants with DE, regardless of pterygium status. In conclusion, HPV infection was not a pathogenic factor of pterygia. The hypothesis of HPV transmitting from the genitals to ocular surfaces was nullified. Tear VEGF was involved in both pterygia and DE, whereas tear IL-6 and IL-18 played roles only in DE.

scholarly journals Distribution and Prevalence of High-Risk Human Papillomavirus Genotypes in Cervical Specimens

2020 ◽  
Vol 25 (3) ◽  
pp. 325-331
Author(s):  
Erkan Özmen ◽  
Ülkü Altoparlak ◽  
Muhammet Hamidullah Uyanık ◽  
Abdulkadir Gülen
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Age Groups ◽  
Hpv Infection ◽  
High Rate ◽  
Genotype Distribution ◽  
Hpv Dna ◽  
Hpv Test ◽  
Significant Difference ◽  
Hpv Types

Introduction: Human papillomavirus (HPV) is frequently a sexually transmitted virus and can cause cervical cancer in women. Cervical cancer is the second most common type of cancer among the developing countries. In this study, cervical HPV DNA positivity and genotype distributions were investigated in female patients living in our region and the results were compared with different studies. Materials and Methods: Between 1 July, 2017 and 1 March, 2019, 433 cervical swabs were sent to Ataturk University, Medical Faculty Hospital, Medical Microbiology Laboratory due to suspicion of HPV. Swab samples were evaluated for HPV virus using molecular (Polymerase Chain Reaction-PCR) methods. For this purpose, Xpert HPV Test (Cepheid, Inc, Sunnyvale, CA) was used to identify HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 t in a single sample. Results: Mean age of the patients ranged from 20 to 69 years, with a mean of 39.8 years (± 10.0). Positivity was detected in 62 of the 433 patients. Mean age of the positive patients was 40.2 years (± 11.3). When the positive patients were examined in terms of HPV types, the presence of HPV 16 was observed with a rate of 25.6%, while the HPV 18/45 types were found to be 9.0% in total. When patients were evaluated according to age groups, HPV DNA positivity was highest in the 25-34 age group with 38.7%. In our statistical study, there was no significant difference in HPV DNA positivity rate between the ages of 35 and under 35 years. Conclusion: This study demonstrates the prevalence and viral genotype distribution of HPV infection in women in Erzurum region. HPV type 16 is seen with a high rate in our region.

Comparing the Lifestyle of Veteran and Non-Veteran Families

2021 ◽  
Author(s):  
Seyed Alireza Afshani ◽  
Seyed Mojtaba Hosseini-motlagh ◽  
Hamide Shiri-Mohammadabad
Keyword(s):  
Health Literacy ◽  
Employment Status ◽  
Survey Method ◽  
Statistical Population ◽  
Significant Difference ◽  
The Status ◽  
Lifestyle Questionnaire ◽  
Mashhad City ◽  
The Mean

Background: Considering the role of lifestyle in promoting, maintaining, continuity health and the importance of paying attention to the veterans and their families who have suffered a lot in order to sacrifice for the homeland, the present study intends to compare the lifestyle among veterans and non-veterans in Mashhad city. Methods: This study was conducted using a survey method. The statistical population included the veteran and non-veteran families of Mashhad. A total of 360 questionnaires were filled out in this city. The research instruments were Lifestyle Questionnaire (LSQ) and All Aspects of Health Literacy Scale. The data were analyzed by SPSS v.24. Results: There is not a significant difference between the mean scores of lifestyle in the two groups of veteran and non-veteran in Mashhad (p > 0.05). The comparison of mean lifestyle scores also showed that there was no significant difference between the lifestyle scores of men and women in the non-veteran group and the mean scores of the single and married lifestyle in the veteran and non-veteran group (p > 0.05). There is a significant relationship between education level and health literacy of veteran and non-veteran families with their lifestyle, age, and employment status. They are also related to the lifestyle of their life because of the status they provide for the sacrifices. Conclusion: Comparison of the two groups in terms of lifestyle indicates that the veterans are in a less favorable position than non-veterans in terms of some aspects of lifestyle such as physical health, exercise and health, disease prevention and mental health.

4. High prevalence of anal HPV infection among HIV-positive male subjects in the absence of anal intercourse

Sexual Health ◽  
2013 ◽  
Vol 10 (6) ◽  
pp. 571
Author(s):  
Eugenio Nelson Cavallari ◽  
Gabriella d'Ettorre ◽  
Giancarlo Ceccarelli ◽  
Sara Serafini ◽  
Alessandra Pierangeli ◽  
...  
Keyword(s):  
Anal Cancer ◽  
Injection Drug Users ◽  
Anal Intercourse ◽  
Hpv Infection ◽  
Hiv Positive ◽  
Receptive Anal Intercourse ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Significant Difference ◽  
History Of

Background The risk of progression to cancer from HPV infection is increased in HIV-positive subjects. Anal HPV infection is a concern among men who have sex with men (MSM), especially in the setting of HIV infection. Methods: Cross-sectional study including 62 HIV-positive injection drug users (IDUs) without history of receptive anal intercourse and 110 HIV-positive MSM. All patients were receiving HAART with HIV-RNA <37 copies/mL. Patients answered a self-administered questionnaire investigating their sexual habits. Participants underwent anal brushing collecting samples for cytology examination (Bethesda 2001 criteria for cervical cytology) and high resolution anoscopy collecting samples for histological classification. HPV-DNA polymerase chain reaction (PCR) identification was performed. Results: Of the IDUs, 62.5% showed CD4 nadir <200 cell/mmc compared with 12% of MSM (P < 0.05), the actual CD4+ T-cells count did not show a statistical difference. HPV-DNA PCR tested positive in 52% of IDUs and 69% of MSM (P < 0.05). Presence of high-risk genotypes did not show significant difference between the groups (38% of IDUs and 31% of MSM). LSIL prevalence was higher among MSM than IDUs (38% v. 23%), HSIL was higher among IDUs than MSM (26% v. 21%). The prevalence of anal cancer was similar in the two populations (3% in each group). All patients, except for men presenting with anal cancer, were completely asymptomatic. Conclusions: Anal HPV infection and anal SIL may be acquired in the absence of anal intercourse in HIV-infected men. The prevalence of HSIL is high among HIV-positive IDUs. All HIV-infected men, regardless of history of anal intercourse, should be considered for anal cytology screening.

scholarly journals Modifications of Golgi Complex in Chondrocytes from Osteoarthrotic (OA) Rat Cartilage

2002 ◽  
Vol 50 (10) ◽  
pp. 1333-1339 ◽  
Author(s):  
Juan B. Kourí ◽  
Lourdes Rojas ◽  
Elizabeth Pérez ◽  
Karin A. Abbud-Lozoya
Keyword(s):  
Monoclonal Antibody ◽  
Endoplasmic Reticulum ◽  
Golgi Complex ◽  
Normal Cartilage ◽  
Golgi Membranes ◽  
Significant Difference ◽  
The Status ◽  
Nuclear Changes ◽  
Oa Chondrocytes

The status of the Golgi complex in normal vs osteoarthrotic (OA) cartilage has not yet been studied. A monoclonal antibody, MAb 58-K-9, allowed scoring of Golgi labeling intensity. In addition, ultrastructural assessment enabled us to focus on the distribution and relation between the endoplasmic reticulum (ER) and Golgi membranes. The study was performed in both normal and partially menisectomized OA-induced rat cartilage 20 and 45 days after surgery. Comparing Golgi immunolabeling intensities (mean ± SEM) revealed a highly significant difference between normal (9.98 ± 1.25), 20-day (2.49 ± 0.34), and 45-day (0.82 ± 0.22) cartilage. Moreover, chondrocytes from normal cartilage displayed 71.18% of labeling intensity in contrast to OA cartilage, in which chondrocyte labeling intensities were 24.95% (20 days) and 8.11% (45 days). OA chondrocytes appeared to display an overall reduction in Golgi labeling intensity, suggesting disruption of this organelle as the OA damage progressed. Interestingly, many 20-day OA-induced chondrocytes exhibited bubble-like Golgi immunolabeling compartmentalizing the cytoplasm, concomitant with putative apoptotic nuclear changes. At the same time, OA chondrocytes with a typical ultrastructural apoptotic pattern revealed a prominent ER gathered together with Golgi vesicles and saccules, also appearing to compartmentalize chondrocyte cytoplasm. We speculate about the role of Golgi modifications and apoptosis in OA pathogenesis.

scholarly journals Interrelation of HPV-infection of endometry carcinoma and its clinical-morphological features

2018 ◽  
Vol 5 (3) ◽  
pp. 75-82
Author(s):  
T. A. Zykova ◽  
T. I. Moiseenko ◽  
E. M. Frantsiyants ◽  
M. A. Vovkochina
Keyword(s):  
Morphological Characteristics ◽  
Tumor Grade ◽  
Hpv Infection ◽  
Disease Stage ◽  
Tissue Samples ◽  
Total Cohort ◽  
First Case ◽  
Tumor Tissues ◽  
The Status ◽  
Formalin Fixed Paraffin Embedded

Background. There are contradictory data on the detection of human papillomavirus (HPV) in the tumor tissue in endometrial cancer (EС). Objective: to assess HPV infection rates in EC tumor tissues and to establish the relationship between the status of HPV infection and tumor morphological characteristics. Materials and methods. 57 formalin-fixed paraffin-embedded (FFPE) tissue samples of EC patients aged 47–78 years were studied. HPV DNAs were found in 54.4 % of samples. Results and conclusion. We did not reveal an association between the HPV tumor status and age, metastasis or invasion depth. However, there was an interdependence between HPV infection and some morphological characteristics of the tumor: its histological type (adenocarcinomas with squamous cell differentiation were HPV-positive 1.8 times more frequent compared to adenocarcinomas without one; in the first case, tumor tissues were more often infected with HPV 16, and in the second case with HPV 18); tumor grade (in the total cohort and in serous-papillary adenocarcinomas, tumors with higher grades were more often HPV-infected: from 0 to 81.8 % and from 0 to 100 % respectively); disease stage (in the total cohort the percentage of HPV-positive tumors in stage II was 2.4 times and in stage III – 1.6 times higher than in stage I, and stage IA tumors were HPV-positive 2.3 times more often than IB tumors); type of tumor growth (tumors with infiltrative growth type were HPV-positive 1.7 times more often than with exophytic growth, and 2.2 times more often than with mixed growth). The achieved results do not allow us to conclude with confidence that HPV is the main tumor forming factor in EC.

scholarly journals Survey, Identification, and Pathogenicity of Ceratocystis fimbriata Complex Associated with Wilt Disease on Acacia mangium in Malaysia

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1782
Author(s):  
Samsuddin Ahmad Syazwan ◽  
Ahmad Mohd-Farid ◽  
Wan-Azhar Wan-Muhd-Azrul ◽  
Hishamuddin Muhammad Syahmi ◽  
Abdullah Mohd Zaki ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Acacia Mangium ◽  
Sensu Stricto ◽  
Wilt Disease ◽  
Ceratocystis Fimbriata ◽  
Ceratocystis Wilt ◽  
Significant Difference ◽  
The Status ◽  
Plantation Species ◽  
Bark Peeling

Ceratocystis wilt disease surveys were conducted in three selected Malaysian Acacia mangium plantations. These completed surveys revealed the occurrence of the wilt disease, with the incidence of infection ranging from 7.5% to 13.6%. Signs of wood-boring insects, bark peeling due to squirrel activity, and pruning wounds were often associated with this disease. The fungus most frequently isolated from the diseased trees was the Ceratocystis fungus. The analysis on the morphological characteristics has identified the fungus as Ceratocystis fimbriata complex. Phylogenetic analysis based on the sequences of the ITS, and concatenated sequences of EF1α-βT regions grouped the isolates within the C. fimbriata sensu stricto, in comparison to other C. fimbriata isolates. Pathogenicity tests were conducted on six to nine-month-old healthy A. mangium seedlings by inoculating these seedlings with eight out of the 16 isolates. The results demonstrated that all the isolates were pathogenic, with mortality beginning as early as two weeks after inoculation. However, an ANOVA test indicated a significant difference between the pathogenicity levels among the fungal isolates. The results also showed that pathogen aggressiveness was not correlated with geographical origin. A host range test was also conducted by using C. fimbriata SSB3 and FRIM1162 isolates against several forest plantation species. The findings suggested that only A. mangium was susceptible to C. fimbriata. The other species remained healthy with no symptoms of infection even after seven weeks of treatment, as compared to the A. mangium species, where between 38 to 60% of the inoculated plants had died. This study provides new information on the status of Ceratocystis wilt disease, especially on the occurrence and effects on A. mangium plantation, by giving insights on how to control and manage this ferocious plant pathogen in the future.

Hybridization between the estuarine fishes yellowfin bream, Acanthopagrus australis (Gunther), and black bream, A. butcheri (Munro) (Pisces : Sparidae)

1984 ◽  
Vol 35 (4) ◽  
pp. 427 ◽  
Author(s):  
SJ Rowland
Keyword(s):  
Morphological Characteristics ◽  
F1 Hybrids ◽  
South Wales ◽  
Significant Difference ◽  
Close Relationship ◽  
Electrophoretic Data ◽  
The Status ◽  
Backcross Hybrids ◽  
Species Specific ◽  
High Level

The status of bream, from estuaries near Narooma on the south coast of New South Wales, which display morphological characteristics intermediate to those distinguishing the species A. australis and A. butcheri was investigated. The two species were separated electrophoretically at a malate dehydrogenase locus (Mdh-1) where they are fixed for alternative alleles, and by a significant difference in allele frequency at the lactate dehydrogenase locus Ldh-A. Because four individual bream, which displayed intermediate morphometric characteristics, have inherited both species-specific Mdh-1 alleles and are heterozygous at the Ldh-A locus, their status as hybrids is determined. The presence of three bream with allelic characteristics of A. australis at the Mdh-1 locus but allelic characteristics of A. butcheri at the Ldh-A locus is evidence for the presence of later generation or backcross hybrids. No evidence of introgression was found. The reproduction of A. australis and A. butcheri is temporally and spatially isolated under normal circumstances but in some landlocked, coastal lakes there is a breakdown of these isolating mechanisms. Because A. australis and A. butcheri differ morphometrically and ecologically over their sampled ranges, and only appear to hybridize in the unusual environmental conditions of closed lakes, it is suggested that their specific status be retained. Using electrophoretic data, a high level of genic similarity (I = 0.91) was found to exist between the two species. This, together with the close relationship indicated by the production of fertile F1 hybrids, suggests a recent speciation.

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