Production of D-Lactic Acid by the Fermentation of Orange Peel Waste Hydrolysate by Lactic Acid Bacteria

Lactic acid is one the most interesting monomer candidates to replace some petroleum- based monomers. The application of conventional poly-lactic acid (PLA) is limited due to insufficient thermal properties. This limitation can be overcome by blending poly-D and poly-L-lactic acid. The main problem is the limited knowledge of D-lactic acid (D-LA) production. Efficient biochemical processes are being developed in order to synthesize D-LA from orange peel waste (OPW). OPW is an interesting renewable raw material for biorefinery processes of biocatalytic, catalytic or thermal nature owing to its low lignin and ash content. Bioprocessing of the pretreated OPW is carried out by enzymatic hydrolysis and fermentation of the released sugars to produce D-LA. Several strains of the species Lactobacillus delbrueckii ssp. bulgaricus have been evaluated for the production of D-LA from OPW hydrolysate using Lactobacillus delbrueckii ssp. delbrueckii CECT 286 as a reference strain since its performance in this kind of substrate have been widely reported in previous studies. Preliminary results show that Lactobacillus delbrueckii ssp. bulgaricus CECT 5037 had the best performance with a yield of 84% w/w for D-LA production and up to 95% (e.e.).

Download Full-text

Production of d-lactic acid by Lactobacillus delbrueckii ssp. delbrueckii from orange peel waste: techno-economical assessment of nitrogen sources

Applied Microbiology and Biotechnology ◽

10.1007/s00253-018-9432-4 ◽

2018 ◽

Vol 102 (24) ◽

pp. 10511-10521 ◽

Cited By ~ 14

Author(s):

I. de la Torre ◽

M. Ladero ◽

V. E. Santos

Keyword(s):

Lactic Acid ◽

Orange Peel ◽

Nitrogen Sources ◽

Lactobacillus Delbrueckii ◽

Orange Peel Waste

Download Full-text

Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

REAKTOR ◽

10.14710/reaktor.5.2.79-83 ◽

2017 ◽

Vol 5 (2) ◽

pp. 79

Author(s):

Abdullah Abdullah ◽

H. B. Mat

Keyword(s):

Lactic Acid ◽

Yeast Extract ◽

Lactic Acid Production ◽

Lactobacillus Delbrueckii ◽

Raw Material ◽

Acid Fermentation ◽

Fermentation Time ◽

Pineapple Waste ◽

Fermentation Liquid ◽

Initial Ph

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

Download Full-text

Crystallization and Thermal Degradation of Green Nanocomposites Based on Lignin Coated Cellulose Nanocrystals and Poly(Lactic Acid)

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.737.256 ◽

2017 ◽

Vol 737 ◽

pp. 256-261 ◽

Cited By ~ 1

Author(s):

Martin Boruvka ◽

Luboš Bĕhálek

Keyword(s):

Lactic Acid ◽

Cellulose Nanocrystals ◽

Raw Material ◽

Degree Of Crystallinity ◽

Poly Lactic Acid ◽

Crystalline Cellulose ◽

Melt Crystallization ◽

Scanning Calorimetry ◽

Wide Range ◽

Twin Screw

Cellulose is almost inexhaustible source of raw material comprising at least one-third of all biomass matter. Through deconstruction of cellulose hierarchical structure can be extracted highly crystalline cellulose nanocrystals (CNC) with impressive properties. However, the main barrier in the processing of the nanocomposites based on CNC is their inhomogeneous dispersion and distribution in the non-polar polymer matrix. In this paper is this problem addressed by use of novel hydrophobic lignin coated CNC as a biobased nucleation agents in poly (lactic acid) (PLA) nanocomposites. These green nanocomposites based on natural plant derived substances have enormous potential to replace materials originated from non-renewable resources and show promise of providing degradation back into the environment when they are no longer needed. Resulted composites prepared by twin screw extrusion and injection moulding were characterized by means of scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The addition of L-CNC (1, 2 and 3 wt. %) into PLA increased melt crystallization enthalpy and decreases the cold crystallization enthalpy. The degree of crystallinity (cc) increased from 5.6 % (virgin PLA) to 8.5 % (PLA/1-L-CNC), 10.3 % (PLA/2-L-CNC) and 10.7 % (PLA/3-L-CNC). The wide range of degradation temperatures of lignin coating has been observed starting at 100 °C.

Download Full-text

Lactic acid production by Lactobacillus sp. from biodiesel derived raw glycerol

CT&F - Ciencia Tecnología y Futuro ◽

10.29047/01225383.213 ◽

2012 ◽

Vol 5 (1) ◽

pp. 57-66 ◽

Cited By ~ 2

Author(s):

Yessica Prada-Palomo ◽

Marly Romero-Vanegas ◽

Pedro Díaz-Ruíz ◽

Daniel Molina-Velasco ◽

Carolina Guzmán-Luna

Keyword(s):

Lactic Acid ◽

Reference Strain ◽

Lactic Acid Production ◽

Value Added ◽

Raw Material ◽

Raw Glycerol ◽

Native Strain ◽

Vegetable Products ◽

Adaptation Phase ◽

Biodiesel Industry

Lactic Acid (LA) is a versatile compound with extensive industrial usage. Lactic Acid Bacteria (LAB) are renowned for their capacity to ferment carbohydrates to LA. In the biodiesel industry, bioconversion of residual glycerol to monomers of biopolymers, such as LA, is an alternative for usage of this waste as raw material to produce a high value added biotechnological compound. This research evaluates LA production through fermentation processes using a Lactobacillus sp. native strain and raw glycerol from the biodiesel industry. Native strain was isolated from the processing of dairy, fruit, and vegetable products and through cultures in Man, Rogosa & Sharpe agar (MRS). After an adaptation phase, the fermentative capability of the strain was evaluated through chemical quantification of metabolites using Nuclear Magnetic Resonance (1H-NMR); fermentative potential was compared with a Lactobacillus sp. reference strain (ATCC 7469). The native strain Lactobacillus sp. CYP4 showed a higher bioconversion potential (LA concentration: 39.41 mM, conversion percentage: 39.27%, at 24 h, volumetric productivity: 1.28 g.L-1.h-1, yield: 0.71 g·g-1) from raw glycerol, when compared to the reference strain.

Download Full-text

Orange peel waste from juicing as raw material for plastic composites intended for use in food packaging

Journal of Applied Polymer Science ◽

10.1002/app.48841 ◽

2020 ◽

Vol 137 (26) ◽

pp. 48841 ◽

Cited By ~ 2

Author(s):

Jack Fehlberg ◽

Chun‐Lung Lee ◽

Laurent M. Matuana ◽

Eva Almenar

Keyword(s):

Food Packaging ◽

Orange Peel ◽

Raw Material ◽

Plastic Composites ◽

Orange Peel Waste

Download Full-text

Solid And Liquid Pineapple Waste Utilization For Lactic Acid Fermentation

REAKTOR ◽

10.14710/reaktor.11.1.50-52 ◽

2017 ◽

Vol 11 (1) ◽

pp. 50 ◽

Cited By ~ 5

Author(s):

Abdullah Abdullah

Keyword(s):

Lactic Acid ◽

Carbon Source ◽

Batch Fermentation ◽

Anaerobic Fermentation ◽

Waste Utilization ◽

Lactic Acid Fermentation ◽

Lactobacillus Delbrueckii ◽

Raw Material ◽

Acid Fermentation ◽

Pineapple Waste

The liquid and solid pineapple waste contain mainly sucrose, glucose,fructose, and other nutrients. It therefore can potentially be used as carbon source for fermentation to produce organic acid. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer, the experiments were carried out in batch fermentation using the liquid and solid pineapple waste to produce lactic acid. The anaerobic fermentation of lactic acis were performed at 40 0C, ph 6, 5% inocolum, and 50 rpm. Initially results show that the liquid pineapple waste by using Lactobacillus delbrueckii can be used as carbon source for lactic acid fermentation. The production of lactic acid are found to be 79% yield, while only 56% yield was produced y using solid waste.Keywords : Lactic acid fermentation, Pineapple waste, Lactobacillus delbrueckii

Download Full-text

Preparation of Urea Slow Release Membrane Environment Friendly

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.482-484.1618 ◽

2012 ◽

Vol 482-484 ◽

pp. 1618-1621 ◽

Cited By ~ 1

Author(s):

Shu Jiang Wang ◽

Xing Quan Wang ◽

Ming Hui Chen

Keyword(s):

Lactic Acid ◽

Sustained Release ◽

Corn Starch ◽

Slow Release ◽

Urea Formaldehyde ◽

Raw Material ◽

Poly Lactic Acid ◽

Dialdehyde Starch ◽

Membrane Absorption ◽

High Efficient

First, the corn starch was taken as raw material, and then it was broken down by enzymes and fermentation by lactic acid bacteria, and the poly lactic acid was obtained by chemical synthesis. Then the corn starch was taken as raw material again, the dialdehyde starch was obtained by high efficient light-catalytic reactor. Secondly, a certain percentage of dialdehyde starch and urea were mixed together to get urea-formaldehyde resin. Finally, a certain amount of polylactic acid, urea formaldehyde resins and dispersions were put together and mixed in a high-speed stirring to get urea slow release membrane materials. Experimental results showed that it needed 150-300 days degradate completely by 75% poly lactic acid sustained release membrane, while 85% needed 100 -120 days. Single thin membrane absorption test showed that per unit area of the sustained release membrane absorption ability would increase with the poly lactic acid increased, and the absorbent capacity of 85% poly lactic acid of sustained release membrane was 112 times higher than the mass of itself.

Download Full-text

Pengasuhan Manajemen Kewirausahaan melalui Pelatihan Pembuatan Sabun Aroma Terapi dari Limbah Kulit Jeruk

Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA ◽

10.21831/jpmmp.v2i2.21915 ◽

2018 ◽

Vol 2 (2) ◽

pp. 87-94

Author(s):

Eli Rohaeti ◽

Sulistyani Sulistyani ◽

Dewi Yuanita Lestari

Keyword(s):

Orange Peel ◽

Marketing Strategies ◽

Raw Material ◽

Processing Waste ◽

Management Activity ◽

Citrus Peel ◽

Soap Making ◽

Entrepreneurial Management ◽

Orange Peel Waste ◽

Training Activities

Kegiatan penyuluhan dan pelatihan melalui PkM ini bertujuan untuk memberikan pengetahuan tentang cara pembuatan sabun mandi aroma terapi dari limbah kulit jeruk pada pemuda Kampung Pingit, menjelaskan manfaat pengolahan limbah kulit jeruk menjadi sabun mandi aroma terapi, dan memotivasi pemuda Kampung Pingit untuk membuat usaha pembuatan sabun mandi aroma terapi. Kegiatan PkM ditujukan bagi kelompok Muda Mudi Mesjid Al-Manshurin Kampung Pingit, Kelurahan Bumijo, Kecamatan Jetis, Kodya Yogya. Melalui koordinasi dengan Ketua Muda Mudi Mesjid Al-Manshurin Kampung Pingit, sebanyak 48 anggota kelompok muda mudi Kampung Pingit diundang dalam pelatihan ini. Metode yang digunakan dalam kegiatan PkM ini adalah ceramah, diskusi, dan demonstrasi tentang cara pembuatan sabun mandi aroma terapi, manfaat pengolahan kulit jeruk menjadi minyak atsiri sebagai bahan baku pembuatan sabun mandi aroma terapi, serta kiat untuk melakukan wirausaha di bidang pembuatan sabun mandi aroma terapi meliputi modal yang dibutuhkan, teknik pengemasan, serta strategi pemasaran. Berdasarkan evaluasi proses dan produk serta diskusi dengan peserta pelatihan kegiatan PPM dapat disimpulkan bahwa seluruh pemuda Kampung Pingit peserta kegiatan pengasuhan manajemen kewirausahaan mengungkap bahwa kegiatan PPM tentang cara pembuatan sabun mandi aroma terapi dari limbah kulit jeruk dapat memberikan bekal pengetahuan dan pemahaman tentang pembuatan sabun mandi aroma terapi. Sebanyak 100% peserta pelatihan menyatakan bahwa kegiatan pengasuhan manajemen kewirausahaan pengolahan limbah kulit jeruk menjadi sabun mandi aroma terapi dapat memberikan manfaat kepada mereka. Sebanyak 91% peserta pelatihan menyatakan bahwa kegiatan pengasuhan manajemen kewirausahaan ini mampu memotivasi pemuda Kampung Pingit untuk mencoba sendiri dan sebanyak 80% peserta pelatihan menyatakan bahwa kegiatan PPM tersebut dapat memotivasi mereka untuk berwirausaha membuat usaha pembuatan sabun mandi aroma terapi. Kata kunci: limbah kulit jeruk, pengasuhan, dan sabun aroma terapi Parenting of Entrepreneurship Management through Training of Making of Aroma Soap from Citrus Skin Waste Abstract Extension and training activities through PkM aimed to provide knowledge on how to make aromatherapy bath soap from citrus peel waste in Pingit Village youth, to explain the benefits of citrus skin processing waste into aromatherapy soap, and to motivate the youth in Pingit Village to make a bath soap making business aroma therapy. The activities of PkM were aimed at youths in Mesjid Al-Manshurin Kampung Pingit, Bumijo, Jetis, Yogya. In coordination with the Chairman of Muda Mudi Mesjid Al-Manshurin of Pingit Village, 48 members of the young in village group were invited to this training. The methods used in this PkM activity were lectures, discussions, and demonstrations on how to make aromatherapy soap, the benefits of citrus skin processing into essential oil as a raw material for aromatherapy soaps, and tips for entrepreneurship in aromatherapy soap making including the required capital, packaging techniques, and marketing strategies. Based on the evaluation of the process and product and discussion with the participants of the PkM activity, it can be concluded that all youth participated in entrepreneurial management activities revealed that the PkM activities on how to make aromatherapy soap bath from orange peel waste can provide knowledge and understanding about making aroma bath soap therapy. As many as 100% of trainees stated that the entrepreneurial management activities of citrus skin processing waste into aromatherapy soap can benefit them. As many as 91% of the participants stated that this entrepreneurial management activity is able to motivate the youth to try their own and as many as 80% of the participants stated that the PkM activities can motivate them to make entrepreneurial effort to make aromatherapy bath soap. Keywords: citron skin waste, parenting, and aromatherapy soap

Download Full-text

STRATEGI IMITASI DAN MANAJEMEN PENGETAHUAN DALAM PENGEMBANGAN INOVASI PLA (POLY LACTIC ACID) PADA AGROINDUSTRIKEMASAN BIODEGRADABEL DI INDONESIA

JURNAL TEKNIK INDUSTRI ◽

10.25105/jti.v3i2.1574 ◽

2013 ◽

Vol 3 (2) ◽

Author(s):

Fitry Filianty

Keyword(s):

Knowledge Management ◽

Lactic Acid ◽

Raw Materials ◽

Raw Material ◽

Poly Lactic Acid ◽

Product Imitation ◽

Imitation Strategy

Indonesia has the potential for development of PLA (Poly Lactic Acid) as biodegradable packaging agroindustry due to the availability of raw material, such as tubers as a source of starch and lignocellulose as a source of cellulose, but Indonesia has limitations in terms of its technology. This was due to the intensity and quality of the research that has not been adequate. For that condition, imitation strategies can be developed for PLA (Poly Lactic Acid) agroindustry in Indonesia and the level of product imitation strategies is knockoff or cloning. At that level, PLA agroindustry to be developed completely replicate existing products but have other brands. With this strategy, the PLA agroindustry is expected to produce a cheaper price product because of the availability of raw materials in Indonesia that easy to fine. Raw material for PLA that can be selected from cassava tubers beacause its easy in cultivation and cheaper among other types of bulbs. To improve competitiveness, the company who built the imitation strategy needs to be managed with the application of knowledge management in order to be able to last long existence.

Download Full-text