scholarly journals Momilactones A, B, and Tricin in Rice Grain and By-Products are Potential Skin Aging Inhibitors

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 602 ◽  
Author(s):  
Nguyen Van Quan ◽  
Dam Duy Thien ◽  
Tran Dang Khanh ◽  
Hoang-Dung Tran ◽  
Tran Dang Xuan
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Biological Activities ◽  
Ultra Performance Liquid Chromatography ◽  
Skin Aging ◽  
Ionization Mass ◽  
Rice Grain ◽  
Synergistic Activity ◽  
Ic50 Values

We previously reported the inhibitory potentials of momilactones A (MA) and B (MB) against key enzymes related to type 2 diabetes and obesity. In this study, antioxidant and anti-skin-aging activities of MA and MB were investigated and compared with tricin, a well-known antioxidant and antiaging flavonoid in rice. MA, MB, and tricin were purified from rice husk by column chromatography and their biological activities were subsequently assayed by in vitro trials. The contents of MA, MB, and tricin of different commercial rice cultivars in Japan were quantified and confirmed by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS) and high-performance liquid chromatography (HPLC) analyses. The antioxidant assays revealed a synergistic activity of the mixture MA and MB (MAB, 1:1, v/v). In addition, in 2,2’-azino-bis (ABTS) assay, IC50 values of MAB (0.3 mg/mL) and tricin (0.3 mg/mL) was 4-fold and 9-fold greater than that of individual MB (1.3 mg/mL) or MA (2.8 mg/mL), respectively. The in vitro enzymatic assays on pancreatic elastase and tyrosinase indicated that MA and MB were potential to relief skin wrinkles and freckles. In detail, MA exerted higher inhibition on both enzymatic activities (30.9 and 37.6% for elastase and tyrosinase inhibition, respectively) than MB (18.5 and 12.6%) and MAB (32.0 and 19.7%) at a concentration of 2.0 mg/mL. Notably, MA and the mixture MAB exhibited stronger inhibitions on elastase and tyrosinase in comparison with tricin and vanillin. MA, MB, and tricin in rice are potential to develop cosmetics as well as supplements for skin aging treatments.

scholarly journals Momilactones A and B Are α-Amylase and α-Glucosidase Inhibitors

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 482 ◽  
Author(s):  
Nguyen Quan ◽  
Hoang-Dung Tran ◽  
Tran Xuan ◽  
Ateeque Ahmad ◽  
Tran Dat ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
In Vitro Assays ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Rice Grain ◽  
Esi Ms ◽  
13C Nuclear Magnetic Resonance

Momilactones A (MA) and B (MB) are the active phytoalexins and allelochemicals in rice. In this study, MA and MB were purified from rice husk of Oryza sativa cv. Koshihikari by column chromatography, and purification was confirmed by high-performance liquid chromatography, thin-layer chromatography, gas chromatography-mass spectrometry, liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and 1H and 13C nuclear magnetic resonance analyses. By in vitro assays, both MA and MB exerted potent inhibition on α-amylase and α-glucosidase activities. The inhibitory effect of MB on these two key enzymes was greater than that of MA. Both MA and MB exerted greater α-glucosidase suppression as compared to that of the commercial diabetic inhibitor acarbose. Quantities of MA and MB in rice grain were 2.07 ± 0.01 and 1.06 ± 0.01 µg/dry weight (DW), respectively. This study was the first to confirm the presence of MA and MB in refined rice grain and reported the α-amylase and α-glucosidase inhibitory activity of the two compounds. The improved protocol of LC-ESI-MS in this research was simple and effective to detect and isolate MA and MB in rice organs.

scholarly journals Phytochemical Study and In Vitro Screening Focusing on the Anti-Aging Features of Various Plants of the Greek Flora

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1206
Author(s):  
Aimilia D. Sklirou ◽  
Maria T. Angelopoulou ◽  
Aikaterini Argyropoulou ◽  
Eliza Chaita ◽  
Vasiliki Ioanna Boka ◽  
...  
Keyword(s):  
Plant Species ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Ultra Performance Liquid Chromatography ◽  
Skin Aging ◽  
Rosa Damascena ◽  
Phytochemical Study ◽  
Age Related

Skin health is heavily affected by ultraviolet irradiation from the sun. In addition, senile skin is characterized by major changes in the collagen, elastin and in the hyaluronan content. Natural products (NPs) have been shown to delay cellular senescence or in vivo aging by regulating age-related signaling pathways. Moreover, NPs are a preferable source of photoprotective agents and have been proven to be useful against the undesirable skin hyperpigmentation. Greek flora harvests great plant diversity with approximately 6000 plant species, as it has a wealth of NPs. Here, we report an extensive screening among hundreds of plant species. More than 440 plant species and subspecies were selected and evaluated. The extracts were screened for their antioxidant and anti-melanogenic properties, while the most promising were further subjected to various in vitro and cell-based assays related to skin aging. In parallel, their chemical profile was analyzed with High-Performance Thin-Layer Chromatography (HPTLC) and/or Ultra-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UPLC-HRMS). A variety of extracts were identified that can be of great value for the cosmetic industry, since they combine antioxidant, photoprotective, anti-melanogenic and anti-aging properties. In particular, the methanolic extracts of Sideritis scardica and Rosa damascena could be worthy of further attention, since they showed interesting chemical profiles and promising properties against specific targets involved in skin aging.

scholarly journals Electrospray ionization mass spectrometry combined with the ultra high performance liquid chromatography in the analysis of in vitro formation of chlorophyll complexes with copper and zinc

2014 ◽  
Vol 79 (6) ◽  
pp. 689-706 ◽  
Author(s):  
Jelena Zvezdanovic ◽  
Sanja Petrovic ◽  
Dejan Markovic ◽  
Tatjana Andjelkovic ◽  
Darko Andjelkovic
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
High Performance ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Central Type

The aim of this work was to give more accurate insight in the interaction of major photosynthesis pigment, chlorophyll (Chl), with copper (II) and zinc (II) in solution by flow injection analysis with electrospray ionization mass spectrometry (FIA-ESI-MS) method, as well as combined with ultra high performance liquid chromatography with DAD detection (UHPLC-DAD). These interactions may potentially, but not necessarily lead to formation of Cu-Chl and Zn-Chl complexes of two different types, which has a lot of - at least - disfunctional implications in plant world. The results based on analysis of full-scan and MS/MS spectra - with or without the UHPLC chromatograms - confirm formation of ?central type? Cu-Chl complex, and ?central type? Zn-Chl complex as well, along with a proof for formation of ?peripheral? Zn-Chl complex, the latter one originating from a very week coordinative interaction at the edge of Chl structure. The used techniques appeared to be an efficient and reliable tool for studying formation and stability of heavy metals complexes with chlorophyll, at least in vitro, with a considerable possibility for assessment of real bio-environmental behavior.

scholarly journals Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of Elaeagnusmultiflora Thunb

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 849
Author(s):  
Sabina Lachowicz-Wiśniewska ◽  
Ireneusz Kapusta ◽  
Carla M. Stinco ◽  
Antonio J. Meléndez-Martínez ◽  
Anna Bieniek ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Photodiode Array Detection ◽  
Fruit Skin ◽  
Liquid Chromatography Tandem Mass

The purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of Elaeagnus multiflora Thunb., as well as their in vitro biological potency. The polyphenols and isoprenoids were determined with UPLC-PDA-MS/MS (ultra-performance liquid chromatography coupled to photodiode array detection and electrospray ionization tandem mass spectrometry) and RRLC-MS/MS (rapid resolution liquid chromatography/tandem mass spectrometry) methods, the organic acid with HPLC-RID (high-performance liquid chromatography coupled to a Refractive Index Detector), and the antioxidant capacity using ABTS and FRAP assays. Enzymatic activity was established as the ability to inhibit α-amylase, α-glucosidase, and pancreatic lipase. Owing to such an effective technique, 88 compounds were recorded, with 17 polyphenolic compounds and 3 isoprenoids identified for the first time in the seeds and leaves of cherry silverberry. In total, 55 compounds were identified in the leaves, 36 in the seeds, and 31 in the fruit skin + pulp. The predominant polyphenol was polymeric procyanidin (66–95% of total polyphenolics), whereas the predominant isoprenoids were chlorophyll b and (all-E)-lycopene. The results of our work noted that there are significant differences in the profiles of several secondary metabolites between the analyzed parts of the plant, and depending on the need, the compounds can be used to develop different innovative food or cosmetic products.

scholarly journals Biotransformation of quercetin, kaempferol and apigenin to monoglycosylated derivatives by in vitro suspension cultures of Astragalus vesicarius ssp. carniolicus

Pharmacia ◽  
2021 ◽  
Vol 68 (2) ◽  
pp. 307-311
Author(s):  
Pavlinka Popova ◽  
Yancho Zarev ◽  
Iliana Ionkova
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Electrospray Ionization ◽  
High Performance ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Ionization Mass

Biotransformation of exogenous substrates quercetin, kaempferol and apigenin by suspension cultures of Astragalus vesicarius ssp. carniolicus to their monoglycosylated derivatives was performed. The maximal enzymatic potential of cells of A. vesicarius ssp. carniolicus was evaluated by different concentrations of substrate exposure. According to quantitative ultra-high performance liquid chromatography-high resolution electrospray ionization mass spectrometry (UHPLC-HR-ESI-MS) analysis, the highest concentration of kaempferol O-glycoside (14.88 nmol/g dry weight, DW), apigenin O-glycoside (10.55 nmol/g DW) and quercetin O-glycoside (150.83 nmol/g DW) was achieved, when suspension cultures were treated with 4 mg/mL kaempferol, 4 mg/mL apigenin and 3 mg/mL quercetin, respectively. The glycosidic products of biotransformation were not detected in the untreated control.

scholarly journals Effects of Pigment Constituents and Their Distribution on Spathe Coloration of Zantedeschia hybrida

HortScience ◽  
2017 ◽  
Vol 52 (12) ◽  
pp. 1840-1848
Author(s):  
Ting Lei ◽  
Yang Song ◽  
Xuehua Jin ◽  
Tianyu Su ◽  
Yiwen Pu
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
Colorimetric Method ◽  
Ultra Performance Liquid Chromatography ◽  
Color Variation ◽  
Ionization Mass ◽  
Photodiode Array Detection ◽  
Total Anthocyanin ◽  
Different Color

The plant Zantedeschia hybrida is colorful and suitable for cut flowers and potted plants. This study employed a colorimetric method for the determination of spathe color phenotypes in 27 Z. hybrida cultivars and classified them into six major color classes. To characterize the coloration mechanism of the Z. hybrida spathe, this study explored the main colorants and pigment distribution using high-performance liquid chromatography (HPLC) with photodiode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS), ultra-performance liquid chromatography/hybrid triple quadrupole linear ion trap mass spectrometry (UPLC-Q-TRAP-MS), and tissue sections. The results showed that flavonoids were colorants in the spathes of different color groups and that cyanidin (Cy) was the main colorant, whereas carotenoids were not detected in the spathe. Total anthocyanin (TA) content was negatively correlated with lightness (L*) of coloration, such that a spathe with a higher TA and thicker pigmented cell layer showed a deeper color; however, there was no correlation between deep coloration in a spathe and flattened upper epidermal cells. The difference in TA was the main reason for the color variation among Z. hybrida of different color groups, whereas the total flavones and flavonols (TF) played a key role in the coloration of the orange and yellow group.

Gut microbiota-mediated deglycosylation of ginsenoside Rb1 in rats: in vitro and in vivo insights from quantitative ultra-performance liquid chromatography-mass spectrometry analysis

2015 ◽  
Vol 7 (15) ◽  
pp. 6173-6181 ◽  
Author(s):  
Kang An ◽  
Zhang Shengjie ◽  
Shan Jinjun ◽  
Di Liuqing
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Biological Activities ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometry Analysis ◽  
Ginsenoside Rb1 ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis

Ginsenoside Rb1, an ingredient of the herbal medicine Panax ginseng, possesses a variety of biological activities.

scholarly journals In Vitro Antifungal Activity of Peltophorum dubium (Spreng.) Taub. extracts against Aspergillus flavus

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 438
Author(s):  
Lucía S. Di Ciaccio ◽  
Alejandra V. Catalano ◽  
Paula G. López ◽  
Dante Rojas ◽  
Diego Cristos ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Antifungal Activity ◽  
Aspergillus Flavus ◽  
High Performance ◽  
Colony Growth ◽  
Ionization Mass ◽  
Esi Ms

Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of different collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 µg/mL, and the differences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and Daidzein.

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