scholarly journals Nitrogen Source Affects the Composition of Metabolites in Pepper (Capsicum annuum L.) and Regulates the Synthesis of Capsaicinoids through the GOGAT–GS Pathway

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 150 ◽  
Author(s):  
Jing Zhang ◽  
Jian Lv ◽  
Jianming Xie ◽  
Yantai Gan ◽  
Jeffrey A. Coulter ◽  
...  

Phytochemical analyses of pepper fruit metabolites have been reported; however, much less is known about the influence of different forms of nitrogen (N), which is critical for plant growth and fruit quality formation. The “Longjiao No. 5” variety (Capsicum annuum L.) grown in Northwestern China was profiled using liquid chromatography–mass spectrometry (LC–MS) coupled with multivariate data analysis to explore the composition of different metabolites in pericarp and placenta, and to investigate the effect of three ammonium (NH4+) to-nitrate (NO3−) ratios (0:100, 25:75, and 50:50). A total of 215 metabolites were obtained by qualitative analysis, where 31 metabolites were the major differential metabolite components of pepper fruits between placenta and pericarp, and 25 among N treatments. The addition of ammonium up-regulated carbohydrates, such as α-lactose and sucrose, as well as phenylalanine lyase (PAL) of placenta tissue. The supply of 25% NH4+–N and 75% NO3−–N exhibited a relatively higher levels of ascorbic acid in pericarp and amino acids, capsaicin, and dihydrocapsaicin in placenta, and led to higher fruit weight among the ammonium-to-nitrate ratios. The expression and activities of glutamic acid synthetase (GOGAT) and glutamine synthetase (GS) that are involved in ammonium assimilation were affected by adjusting the ammonium–N proportion, and they were significantly positively correlated with capsaicin, dihydrocapsaicin contents, capsaicinoid synthetase (CS), as well as the relative expression levels of genes related to capsaicinoid biosynthesis, such as acyltransferase 3 (AT3) and acyl-ACP thioesterase (FatA).

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 374 ◽  
Author(s):  
Marta Rodríguez-Ruiz ◽  
Salvador González-Gordo ◽  
Amanda Cañas ◽  
María Jesús Campos ◽  
Alberto Paradela ◽  
...  

During the ripening of sweet pepper (Capsicum annuum L.) fruits, in a genetically controlled scenario, enormous metabolic changes occur that affect the physiology of most cell compartments. Peroxisomal catalase gene expression decreases after pepper fruit ripening, while the enzyme is also susceptible to undergo post-translational modifications (nitration, S-nitrosation, and oxidation) promoted by reactive oxygen and nitrogen species (ROS/RNS). Unlike most plant catalases, the pepper fruit enzyme acts as a homodimer, with an atypical native molecular mass of 125 to 135 kDa and an isoelectric point of 7.4, which is higher than that of most plant catalases. These data suggest that ROS/RNS could be essential to modulate the role of catalase in maintaining basic cellular peroxisomal functions during pepper fruit ripening when nitro-oxidative stress occurs. Using catalase from bovine liver as a model and biotin-switch labeling, in-gel trypsin digestion, and nanoliquid chromatography coupled with mass spectrometry, it was found that Cys377 from the bovine enzyme could potentially undergo S-nitrosation. To our knowledge, this is the first report of a cysteine residue from catalase that can be post-translationally modified by S-nitrosation, which makes it especially important to find the target points where the enzyme can be modulated under either physiological or adverse conditions.


The objective of this research was to evaluate the effect of organic fertilization, compared to inorganic and a combination of both in the cultivation of serrano pepper (Capsicum annuum L.) under shade net at the University of Sonora. The variables that were evaluated were: fruit weight, yield, length and diameter of fruit, as well as efficiency of water use. For the weight of the fruit per plant, no significant differences were observed between treatments in the first two cuts, however, for the third cut if there were significant differences, the best treatments were inorganic fertilization and the mixture of inorganic plus algae extracts with 280.29 and 212.21 g respectively. For the fourth and fifth cut the trend was in the same way up to the final average resulting in the best treatment inorganic fertilization with 233.94 g per plant followed by the mixture of inorganic plus seaweed extract with 166.75 g per plant. Regarding the yield, it can be observed that from the third cut the treatments that contained inorganic resulted with the highest yield, resulting in the total average inorganic fertilization with 38665.77 kg ha-1 and inorganic fertilization plus algae extract with 27514.08 kg ha-1. The results obtained from this research show that organic fertilization does not provide high yields compared to inorganic because of the gradual release of nutrients to the plant.


2019 ◽  
Vol 1 (1) ◽  
pp. 54
Author(s):  
Kana Dau Sukmawati ◽  
Muhamad Syukur ◽  
Arya Widura Ritonga

ABSTRACT Chili pepper (Capsicum annuum L.) is a commodity that has a high economic value and has been known and cultivated by people throughout the world. Ornamental chili pepper has aesthetic value as ornamental plants such as the round or long round fruits and the diversity of fruit colors. This study was conducted to evaluate the qualitative and quantitative characters of 11 genotypes of ornamental chili pepper to obtain superior ornamental chili pepper varieties. The analyzed variables were the plant height, stem length, stem diameter, stem color, shortened internode, plant habitus, leaf shape, leaf color, leaf length, leaf width, days to flowering, corolla color, anther color, flower position, harvest date, fruit weight, fruit length, fruit pedicel length, fruit diameter, fruit shape, fruit shape at blossom end, fruit color, and anthocyanin coloration. This study was conducted in green house of the Cikabayan Experimental Garden and the Laboratory of Genetics and Plant Breeding, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, from January to June 2018. The experimental design was randomized complete block design with one factor (genotipe) and four replicates. All genotypes evaluated had diverse quantitative and qualitative characters especially in the character of fruit weight, fruit length, and fruit color. The most preferred genotypes were Ayesha 1, Violeta, and Nazla. Ayesha 1 was favored because of its rounded fruit, interesting and quite varied fruit colors. Violeta and Nazla were prefered because of the purple fruits. Nazla was prefered as pot ornamental plants for its short plant and the convenience to take care. Keywords: Capsicum annuum L, genotipe, ornamental chili pepper  Cabai (Capsicum annuum L.) merupakan komoditas yang memiliki nilai ekonomi tinggi dan merupakan tanaman yang sudah dikenal dan dibudidayakan oleh masyarakat di seluruh dunia. Karakter cabai hias memiliki nilai estetika sebagai tanaman hias seperti bentuk buah yang umumnya bulat atau bulat panjang dan warna buah yang sangat bervariasi. Penelitian ini dilakukan untuk mengevaluasi karakter kualitatif dan kuantitatif 11 genotipe cabai hias untuk menghasilkan varietas cabai hias yang unggul. Karakter yang diamati terdiri atas tinggi tanaman, tinggi dikotomus, diameter batang, warna batang, pemendekan ruas, habitus tanaman, bentuk daun, warna daun, panjang daun, lebar daun, waktu munculnya bunga, warna mahkota bunga, warna anther, kedudukan bunga, umur panen buah, bobot buah, panjang buah, panjang tangkai buah, diameter buah, bentuk buah, bentuk pangkal buah, perubahan warna buah, dan pewarnaan antosianin. Penelitian dilakukan di rumah kaca Kebun Percobaan Cikabayan dan Laboratorium Genetika dan Pemuliaan Tanaman, Departemen Agronomi dan Hortikultura, Fakultas Pertanian Institut Pertanian Bogor. Penelitian dilaksanakan pada bulan Januari sampai Juni 2018. Rancangan penelitian yang digunakan adalah Rancangan Kelompok Lengkap Teracak (RKLT) satu faktor yaitu genotipe dengan 4 kali ulangan. Semua genotipe yang dievalusi memiliki karakter kuantitatif dan kualitatif yang beragam baik antar genotipe uji maupun dengan varietas pembanding, terutama pada karakter bobot buah, panjang buah, dan warna buah. Genotipe yang paling disukai adalah Ayesha 1, Violeta, dan Nazla. Ayesha 1 disukai karena bentuk buahnya yang membulat dan warna buahnya yang menarik dan beragam. Violeta dan Nazla disukai karena buahnya berwarna ungu. Nazla lebih disukai untuk tanaman hias pot karena perawakannya yang pendek dan memudahkan perawatan. Kata kunci: Capsicum annuum L., cabai hias, genotipe


2004 ◽  
Vol 10 (1) ◽  
Author(s):  
Sz. Jakusné Sári

In spite of the several good properties of peat, recently, some experiments were carried out with the aim of finding natural materials which can substitute for peat. According to the results, several inorganic and organic materials were proved to be suitable for this purpose. This study examines the effect of different organic materials (example: pine bark, composts, peats) on the growth and yield of green pepper (Capsicum annuum L., variety Danubia). We found that the most developed plants were grown in peat-mixtures and pine bark. The average fruit weight was the highest at those plants which were planted also in these media. The plants which were grown in composts fell short of our expectations in development and in yield, too.


OALib ◽  
2021 ◽  
Vol 08 (03) ◽  
pp. 1-20
Author(s):  
Hand Mathias Julien ◽  
Nono Giles Vivien ◽  
Tonfack Libert Brice ◽  
Taffouo Victor Désiré ◽  
Youmbi Emmanuel

2020 ◽  
Vol 4 (2) ◽  
pp. 150-156
Author(s):  
M. R. I. Suhel ◽  
N. J. Methela ◽  
R. A. Ruhi ◽  
B. Hossain

Salinity is one of the major issues that lead to a reduction in crop productivity. This study aimed to investigate the response of sweet pepper (Capsicum annuum L.) to saline soil. The research was conducted in Agricultural Research Field at Noakhali Science and Technology University during 2018-19 with two sweet pepper varieties as treatment named Red Army (V1) and California Wonder (V2). The results indicated that there were significant variations between the two varieties in different parameters of growth as well as yield performance. The result showed that between the treatments V2 (California Wonder) gave the higher yield ie., 10.65 ton/ha while V1(Red Army) produced 9.61 ton/ha. Moreover, the taller plants and longer fruit were obtained from the V2 which were 32.43 cm and 8.23 cm respectively. Even among the other parameters, V2 gave higher growth and development than V1. Furthermore, average single fruit weight/plant was recorded 76.33 g in California Wonder (V2) whereas Red Army (V1) provided 74.16 g. The finding of the experiment indicated that the overall better performance could be obtained from V2 California Wonder variety of sweet pepper. This study evaluated the varietal performance of two varieties and it can be said from the experiment that the California Wonder is the best variety for cultivation in saline region in Bangladesh.


1999 ◽  
Vol 79 (3) ◽  
pp. 411-416 ◽  
Author(s):  
Peter M.A. Toivonen ◽  
P.A. Bowen

The effect of preharvest calcium sprays on postharvest quality of sweet bell peppers grown in plasticulture was studied. Calcium was applied as three sprays of 0.4% (wt/vol) CaCl2 in water to plots of Oriole and Bell Boy peppers in each of 2 yr. Fruit weight, water loss, firmness, decay and the composition of the pectic fractions were determined at harvest and during storage. Preharvest sprays of CaCl2 increased the weight of fruit for Oriole, improved firmness retention in storage, decreased decay, increased pericarp wall thickness and the insoluble pectic fractions of both cultivars but had no effect on water loss in storage. The results suggest that Ca improves firmness retention through enhancing the pectic composition of cell walls and pericarp wall thickness. Effects of Ca sprays on reducing decay could not be attributed to factors measured in this work. Preharvest foliar sprays of CaCl2 show promise in reducing postharvest decay and improving firmness retention in sweet bell peppers grown under plasticulture. Key words: Capsicum annuum L., calcium, pectin, shelf life, postharvest decay


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