scholarly journals Optical Properties and Antioxidant Activity of Water-Ethanolic Extracts from Sempervivum tectorum L. from Bulgaria

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 520
Author(s):  
Galia Gentscheva ◽  
Irina Karadjova ◽  
Stefka Minkova ◽  
Krastena Nikolova ◽  
Velichka Andonova ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Correlation Coefficients ◽  
Large Family ◽  
Ethanol Extract ◽  
Strong Relationship ◽  
Pharmaceutical Products ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Folk Remedy ◽  
Ethanolic Extracts

Sempervivum tectorum L. is an evergreen plant belonging to a large family of the Crassulaceae. The fresh juice of the plant is used as a folk remedy almost exclusively for external purposes. The combination of several instrumental methods—transmission and fluorescence spectroscopy, ICP-MS spectrometry, and assays for the assessment of antioxidant activities were used for the characterization of water-ethanolic extracts from the leaves of Sempervivum tectorum L. with different polarities (ethanol content in the extracts varied between 10% and 95%). The highest total phenolic content was found in the 50% ethanol extract—0.84 ± 0.08 mg GAE/mL. Furthermore, this extract also possessed the highest antioxidant potential evaluated by DPPH and FRAP assays—7.02 ± 0.71 mM TE/mL and 6.15 ± 0.25 mM TE/mL, respectively. High correlation coefficients were found between the total phenolic contents and the antioxidant activities of water-ethanolic extracts from Sempervivum tectorum L. The same is true for the strong relationship between the phenolic contents and the concentrations of Na and K. Most likely, the bioavailable species of elements such as Fe, Zn, Ca, and Mg are mostly aqueous soluble. For all the studied extracts, the toxic element (As, Cd, Pb, Tl, Hg) contents are very much below the permissible limits for pharmaceutical products. On the contrary, the concentrations of compounds such as β-carotene and chlorophyll increase with the increase in ethanol in the extract. Results from this study may be used for the preliminary prognosis of pharmaceutical applications of extracts from Sempervivum tectorum L.

scholarly journals Gümüşhane İlinde Tıbbi Amaçla Kullanılan Atkuyruğu (Equisetum arvense) Bitkisinin Bazı Biyolojik Aktivitelerinin İncelenmesi

2018 ◽  
Vol 5 (13) ◽  
pp. 1810
Author(s):  
Tuba Acet ◽  
Kadriye Özcan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Equisetum Arvense ◽  
Phenolic Contents ◽  
Trolox Equivalent ◽  
Ethanol Extracts ◽  
Gallic Acid Equivalent ◽  
Mic Value

In this study, total phenolic content, antimicrobial and antioxidant activities of the extracts obtained with different polarity solvents of horsetail (Equisetum arvense) plant, which is being used by people in Gümüşhane province for different medical purposes, were investigated. Total phenolic contents were measured by spectrophotometric method as gallic acid equivalent. Antimicrobial activity was determined by disc diffusion and microdilution (MIC value) methods and antioxidant activity was determined by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods and calculated as trolox equivalent. The highest content of phenolic was detected in the ethyl acetate extract (108.9 mg GAE/g extract), while the highest antioxidant capacity was found 15.76 μg/ml as trolox equivalent in the ethanol extract. In addition, the highest antimicrobial activity was detected in the ethanol extracts against to MRSA with 4 μg/ml MIC value.

scholarly journals Antioxidant Activities and Estimation of Phenol and Flavonoid Contents in The Extracts Of Trema Orientalis Linn Blume

2021 ◽  
Vol 41 (2) ◽  
pp. 73-84
Author(s):  
A. Saleh ◽  
S.M. Zainal-Ariffin ◽  
S.F. Yahaya ◽  
A.G. Khaleel
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Contents ◽  
Scavenging Effect ◽  
Inflammatory Conditions ◽  
Trema Orientalis

Phytochemical analysis is important in the evaluation of bioactive compounds from plants. Oxidative stress has been implicated in the pathology of many diseases such as atherosclerosis, rheumatoid arthritis, cancer, cataract, diabetes, cardiovascular diseases, chronic inflammatory conditions, and stroke. The aim of this study was to evaluate polyphenolic content and antioxidant activity of Trema orientalis. Antioxidant activity was estimated spectrophotometrically using 2,2– diphenyl–1–picrylhydrazyl radical scavenging method. The total polyphenolic and flavonoid contents of the Trema orientalis extracts were determined using standard methods. IndependentSample T–test was used for Data analyses. Phytochemical screening revealed the presence of saponins, tannins, steroids, cardiac  glycosides, alkaloids, triterpenes, flavonoids and phenolic compounds. Total phenolic contents were found to be 260.96±2.31 mg GAE/g and 134.08±0.56 mg GAE/g in the ethanol and aqueous extracts respectively. Similarly, total flavonoid contents were between 32.71±0.89 and 4.70±0.23 mg GAE/g. The radical scavenging effect was observed in ethanol extract with IC50 = 9.27 µg/mL. The  abundance of polyphenolic compounds and antioxidant activities of the T. orientalis could confirm their good therapeutic potentials inethnobotany. Keywords: Trema orientalis, Phenols, Flavonoids, Antioxidant activities

Determination of Antioxidant Activities and Total Phenolic Contents of Three Cyanobacterial Species

2014 ◽  
Vol 625 ◽  
pp. 826-829 ◽  
Author(s):  
Hamdy Elsayed Ahmed Ali ◽  
Sanaa Mahmoud Metwally Shanab ◽  
Mervat Aly Mohamed Abo-State ◽  
Emad Ahmed Ahmed Shalaby ◽  
Usama Mohamed Nour El Demerdash ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Correlation Coefficients ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Cyanobacterial Species ◽  
Dpph Scavenging

The antioxidant activity and total phenolic content of different extracts (hexane, chloroform, ethyl acetate, ethanol (70%) and water) of three tested cyanobacterial species (Phormidium sp., Oscillatoria sp. and Nostoc muscorum ) were evaluated, using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging method and Folin–Ciocalteu method, respectively. The ethanolic extract of Oscillatoria sp. possessed the highest antioxidant activity (69.13±0.35 %) and therefore could be potential source of natural antioxidants. The correlation coefficients between the antioxidant activities and the phenolic contents were very small in most of extracts (R2 = 0.0291-0.2934). However, the water extract demonstrated relatively higher value (R2= 0.7446). Thus, phenolic compounds may not be considered the major contributors for the antioxidant activities of these cyanobacterial species. So, other antioxidant substances may be present in the extract and share in this activity.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
E. S. Prasedya ◽  
A. Frediansyah ◽  
N. W. R. Martyasari ◽  
B. K. Ilhami ◽  
A. S. Abidin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Particle Sizes ◽  
Epicatechin Gallate ◽  
Particle Size Reduction ◽  
Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

scholarly journals Bioactive Compounds, Antioxidant, and Antibacterial Properties of Lonicera caerulea Berries: Evaluation of 11 Cultivars

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 624
Author(s):  
Laima Česonienė ◽  
Juozas Labokas ◽  
Ina Jasutienė ◽  
Antanas Šarkinas ◽  
Vilma Kaškonienė ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Vitamin C ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Lonicera Caerulea ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Ethanolic Extracts

The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.

scholarly journals Phenolic Profiles and Antioxidant Activities of 30 Tea Infusions from Green, Black, Oolong, White, Yellow and Dark Teas

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 215 ◽  
Author(s):  
Cai-Ning Zhao ◽  
Guo-Yi Tang ◽  
Shi-Yu Cao ◽  
Xiao-Yu Xu ◽  
Ren-You Gan ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Dry Weight ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Tea Infusions ◽  
Phenolic Profiles ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power

Tea is among the most consumed drink worldwide, and its strong antioxidant activity is considered as the main contributor to several health benefits, such as cardiovascular protection and anticancer effect. In this study, the antioxidant activities of 30 tea infusions, which were obtained by the mimic of drinking tea of the public, from green, black, oolong, white, yellow and dark teas, were evaluated using ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) assays, ranging from 504.80 ± 17.44 to 4647.47 ± 57.87 µmol Fe2+/g dry weight (DW) and 166.29 ± 24.48 to 2532.41 ± 50.18 µmol Trolox/g DW, respectively. Moreover, their total phenolic contents (TPC) were detected by Folin-Ciocalteu assay and were in the range of 24.77 ± 2.02 to 252.65 ± 4.74 mg gallic acid equivalent (GAE)/g DW. Generally, Dianqing Tea, Lushan Yunwu Tea, and Xihu Longjing Tea showed the strongest antioxidant activities among 30 teas. Furthermore, the phenolic compounds in tea infusions were identified and quantified, with catechins most commonly detected, especially in green tea infusions, which were main contributors to their antioxidant activities. Besides tea polyphenols, considerable content of caffeine also presented in 30 tea infusions.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT POTENTIAL OF TRIBULUS TERRESTRIS FRUIT AND MESUA FERREA FLOWER EXTRACTS: A COMPARATIVE STUDY

2018 ◽  
Vol 10 (3) ◽  
pp. 70
Author(s):  
Dakshayini P. N. ◽  
Mahaboob Basha P.
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Pharmaceutical Products ◽  
Free Radical Scavenging ◽  
Aluminium Chloride ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
P Value ◽  
Tribulus Terrestris ◽  
Ethanolic Extracts

Objective: This study aimed to investigate the antioxidants and free radicals scavenging properties of ethanolic extracts from Tribulus terrestris fruit (TTF) and Mesua ferrea flower (MFF).Methods: The antioxidant and free radical scavenging activities were measured by using standard protocols against phytoextracts used at 50, 100, 150, 200 and 250 µg/ml concentration. Total phenolic and flavonoid content were estimated using Folin-Ciocalteu and aluminium-chloride reagent assay methods.Results: Among the extracts, MFF showed the highest total antioxidant ability followed by TTF. With regard to various scavenging activities, MFF had the highest radical scavenging activity than TTF extract closely resembled the standards. Also, the reducing capacity on ferrous ion was high in MFF than TTF. The quantified phenolic contents of TTF and MFF ethanolic extracts were 27.0±0.36 and 57.0±0.32 mg gallic acid equivalent per gram extract respectively. A positive correlation (p value<0.001) was observed between phenolic content and free radical scavenging efficiencies.Conclusion: The results confirm that both MFF and TTF are important sources of natural antioxidants and serves as an effective free radical scavengers and/or inhibitors. Hence, both extracts are suitable plant-based pharmaceutical products can be used to handle 'free radical-mediated diseases.

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