scholarly journals Salmonella enterica Serovar Enteritidis Control in Poultry Litter Mediated by Lytic Bacteriophage Isolated from Swine Manure

Author(s):  
Paula Rogovski ◽  
Raphael da Silva ◽  
Rafael Dorighello Cadamuro ◽  
Estêvão Brasiliense de Souza ◽  
Beatriz Pereira Savi ◽  
...  

We report the use of bacteriophages for control of Salmonella Enteritidis in poultry production. Phage was isolated by the double-agar plate assay from agricultural waste samples, and one isolate, named SM1, was selected and propagated for application in poultry litter. Two experimental protocols were tested: single treatment and repeated treatment (re-application of phage SM1 after 6 h and 12 h). Each treatment cycle involved 25 g of poultry litter placed in plastic boxes and contaminated with 105 Colony Forming Units mL−1 (CFU mL−1) of S. Enteritidis, in independent duplicates. The contaminated litter was treated with 106 Plaque Forming Units mL−1 (PFU mL−1) of SM1 phage by dripping. Repeated application of phage SM1 reduced Salmonella counts by over 99.9%; the phage persisted in poultry litter for over 35 days. This study illustrates the application of SM1 treatment as a promising technology for bacterial control in production matrices that could allow safe and sustainable use of agricultural waste products as biofertilizers.

2020 ◽  
Vol 62 (4) ◽  
pp. 88-93
Author(s):  
Larisa V. Pilip ◽  
◽  
Maria E. Kazakova ◽  

According to the Federal Waste Classification Catalogue swine manure and slurry are classified as hazardous wastes of class 3. When stored these wastes emit various gases into the atmosphere including greenhouse gases. It is possible to solve this problem by using a chemical approach. For binding pollutants of acidic nature it is proposed to use sodium hypochlorite showing the properties of bases, while for the substances having basic properties it is possible to use sulfuric acid. The distinctive feature of this method is the technology of using the wastes of chemical industry. This paper proposes and justifies the technological scheme of the chemical method for cleaning the air polluted by emissions from industrial pig enterprises. The research was performed under laboratory conditions, taking into account the technological specifics of pig industry. In the course of the study, gravimetric and potentiometric methods were used. The object of the study was native manure obtained from 4-month-old pigs. In the experiment, we used waste sulfuric acid and alkaline solution of sodium hypochlorite produced By "HaloPolymer Kirovo-Chepetsk". The handbook of best available methods in pig industry recommends using low-waste technologies, while it is possible to use waste products from local chemical enterprises for recycling agricultural waste. The technological solution will make it possible to reduce the concentration of odorigenic substances in the air of livestock premises, to eliminate odors from ventilation emissions, to reduce the toxicity of manure effluent and to process toxic fresh pig manure into granular organic fertilizer. The introduction of this scheme into industrial pig farming will dramatically reduce the amount of malodorous animal waste, significantly reduce odor pollution and improve the environmental situation in the areas adjacent to pig farms.


Author(s):  
F. N. Shubin ◽  
A. V. Rakov ◽  
N. A. Kuznetsova ◽  
T. V. Yakubich ◽  
I. P. Snetkova

Aim. Study plasmid characteristics of S. enteritidis strains in patients and features of epidemiology of the infection in regions with incomplete supply of population with local poultry production. Materials and methods. Plasmid analysis of microbe strains isolated from 382 patients and 8 samples of products was carried out, and significance of plasmid types in population morbidity was evaluated. Identification of salmonella was carried out by conventional methods, plasmid specter - by Kado C.I. and Liu S.T. (1981) method. Results. 98.4% of strains contained virulence plasmid p38, and 80.1% of strains also had small plasmids. Sakhalin strains were divided into 16 plasmid types (D=0.794), and strains from Jewish AO - 10 (D=0.834). Uniformity of strains in patients during infection outbreaks and in transmission factors was detected. Conclusion. Features of salmonellosis in the studied subjects of Russian Federation are determined by higher risk of import of products containing salmonella. Monitoring based on plasmid analysis is an effective base for epidemiologic control.


Author(s):  
Adrina Rosseira ◽  
Noor Nabilah Sarbini ◽  
Izni Syahrizal Ibrahim ◽  
Nor Hasanah Abdul Shukor Lim ◽  
Abdul Rahman Mohd Sam ◽  
...  

2020 ◽  
Vol 63 (2) ◽  
pp. 445-454 ◽  
Author(s):  
Qitao Cao ◽  
Wanqin Zhang ◽  
Yunhao Zheng ◽  
Tianjing Lian ◽  
Hongmin Dong

HighlightsThe maximum SCCA concentration was achieved at a C/N ratio of 25 with 18 days of fermentation.The highest VFAs and LA were produced at C/N ratios of 12.7 and 35, respectively, after 12 days of fermentation.The unit energy value of SCCAs for drop-in fuel production was 50.5% higher than that of methane.The dominant bacteria changed from Clostridium to Lactobacillus with increasing C/N ratio.Abstract. Advancing technologies to produce short-chain carboxylic acids (SCCAs) from agricultural waste are expected to be more economical and efficient. This study presented a new attempt to enhance SCCA production from co-digestion of swine manure and corn silage with the carbon-nitrogen (C/N) ratio adjusted from 12.7 to 50.2. Results showed that the maximum SCCA concentration of 26,214 ±2948 mg COD L-1 was achieved at a C/N ratio of 25 with 18 days of digestion, and the concentrations of lactic acid (LA) and volatile fatty acids (VFAs) were 8700 ±1060 mg COD L-1 and 17,514 ±1888 mg COD L-1, respectively. The LA content first increased and then slightly decreased with increasing C/N ratio, and the maximum LA concentration was 468.5 ±44.3 mg COD g-1 VSadded (VSadded means added fermentation material is calculated as volatile solids) at a C/N ratio of 35 at 12 days of digestion. VFAs showed an opposite trend, with a maximum VFA concentration of 601.1 ±42.4 mg COD g-1 VSadded found at a C/N ratio of 12.7 after 12 days of digestion. Potential energy analysis of the highest SCCA production showed that the unit energy value of SCCAs for drop-in fuel production was 50.5% higher than that of methane. Analysis of the microbial community showed that the dominant bacterial groups changed gradually from butyric acid-producing bacteria (Clostridium) to lactic acid-producing bacteria (Lactobacillus) as the C/N ratio increased from 12.7 to 50.2. Keywords: Anaerobic digestion, Corn silage, Lactic acid, Short-chain carboxylic acids, Swine manure.


2012 ◽  
Vol 32 (2) ◽  
pp. 405-414 ◽  
Author(s):  
Adriane de A. Silva ◽  
Adriana M. da Costa ◽  
Regina M. Q. Lana ◽  
Ângela M. Q. Lana

The utilization of organic wastes represents an alternative to recover degraded pasture. The experiment aimed to assess the changes caused by the provision of different organic waste (poultry litter, turkey litter and pig manure) in a medium-textured Oxisol in Brazilian Savanna under degraded pasture. It was applied different doses of waste compared to the use of mineral fertilizers and organic mineral and evaluated the effect on soil parameters (pH, organic matter, phosphorus and potassium) and leaf of Brachiariadecumbens (crude protein, phosphorus and dry mass production). It was observed that application of organic waste did not increase the level of soil organic matter and pH in the surface layer, and the application of turkey litter caused acidification at depths of 0.20-0.40 m and 0.40-0.60 m. There was an increase in P and K in the soil with the application of poultry litter and swine manure. All organic wastes increased the productivity of dry matter and crude protein and phosphorus. The recycling of nutrients via the application of organic waste allows efficiency of most parameters similar to those observed with the use of mineral sources, contributing to improving the nutritional status of soil-plantsystem.


2001 ◽  
Vol 30 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Kezelee Jones ◽  
Gerard D'Souza

We explore the transfer of poultry litter among watersheds incorporating both economic characteristics (litter demand and supply) and environmental characteristics (vulnerability to phosphorus runoff, a major pollutant). A combination of techniques was employed: the Lemunyon-Gilbert P-Index model to determine watershed environmental vulnerability, GIS for land use coverages, and a goal focusing model (incorporating Saaty's eigen-value approach for penalty weight estimation) to identify optimal litter shipments among watersheds. Both primary and secondary data were used. The results should be useful to producers and policy makers in the study area and in other areas where poultry production is linked to water quality, and contribute to a more sustainable poultry sector.


2019 ◽  
Vol 11 (22) ◽  
pp. 6515 ◽  
Author(s):  
Katarzyna Anna Koryś ◽  
Agnieszka Ewa Latawiec ◽  
Katarzyna Grotkiewicz ◽  
Maciej Kuboń

Adequate management of biomass residues generated by agricultural and food industry can reduce their negative impacts on the environment. The alternative use for agricultural waste is production of biogas. Biomass feedstock intended as a substrate for the agricultural biogas plants may include energy crops, bio-waste, products of animal and plant origin and organic residues from food production. This study reviews the potential of selected biomass residues from the agri-food industry in terms of use for agricultural biogas production in Poland. The most common agri-food residues used as substrates for biogas plants in Poland are maize silage, slurry, and distillery waste. It is important that the input for the agricultural biogas installations can be based on local wastes and co-products that require appropriate disposal or storage conditions and might be burdensome for the environment. The study also discusses several limitations that might have an unfavourable impact regarding biogas plants development in Poland. Given the estimated biomass potential, the assumptions defining the scope of use of agricultural biogas and the undeniable benefits provided by biogas production, agricultural biogas plants should be considered as a promising branch of sustainable electricity and thermal energy production in Poland, especially in rural areas.


2014 ◽  
Vol 600 ◽  
pp. 240-249
Author(s):  
Everton Jose da Silva ◽  
Maria Lidiane Marques ◽  
Antonio Rogério B. Vasconcelos ◽  
Jorge L. Akasaki ◽  
Mauro M. Tashima ◽  
...  

Nowadays, the reuse of waste products in the construction process is a priority research area. Several industrial and agricultural waste products have been investigated, such as fly ash, sugar cane bagasse ash and rice husk ash. This paper analyzes a very important aspect under intense discussion in the scientific community: the Rice Husk Ash (RHA) grinding process. This paper investigates a low carbon RHA with high pozzolanic reactivity produced under uncontrolled burning conditions. The compressive strength of mortar specimens prepared using both ground and natural RHA were tested for 3-56 days and the capillarity absorption was measured for mortars cured during 28 days. Very promising and interesting results were obtained using natural rice husk ash in the production of blended mortars.


Author(s):  
R SRINIVASAN ◽  
K Sathiya

Utilization of industrial and agricultural waste products in the industry has been the focus of research for economical, environmental, and technical reasons. Sugar-cane bagasse is a fibrous waste-product of the sugar refining industry, along with ethanol vapor. This waste-product (Sugar-cane Bagasse ash) is already causing serious environmental pollution which calls for urgent ways of handling the waste. Bagasse ash mainly contains aluminum ion and silica. In this paper, Bagasse ash has been chemically and physically characterized, and partially replaced in the ratio of 0%, 5%, 15% and 25% by weight of cement in concrete. The Fresh concrete tests like compaction factor test, Slump cone test was obtained and hardened concrete tests like compressive strength, split tensile strength, flexural strength and Modulus of Elasticity at the age of 7 and 28 days was obtained. The result shows the increases in percentage of bagasse ash replacement, strength also increased.


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