scholarly journals Occurrence of Antibiotic-Resistant Staphylococcus spp. in Orange Orchards in Thailand

2021 ◽  
Vol 19 (1) ◽  
pp. 246
Author(s):  
Siwalee Rattanapunya ◽  
Aomhatai Deethae ◽  
Susan Woskie ◽  
Pornpimol Kongthip ◽  
Karl R. Matthews
Keyword(s):  
Human Health Risk ◽  
Control Plant ◽  
Bacterial Disease ◽  
Antibiotic Resistant ◽  
Production Environments ◽  
Open Production ◽  
Orange Orchard ◽  
Susceptibility Profiles ◽  
Staphylococcus Spp ◽  
Orange Trees

Background: The widespread indiscriminate application of antibiotics to food crops to control plant disease represents a potential human health risk. In this study, the presence of antibiotic-resistant staphylococci associated with workers and orange orchard environments was determined. A total of 20 orchards (orange and other fruits) were enrolled in the study. Trees in the orange orchards were treated with ampicillin on a pre-determined schedule. Environmental samples (n = 60) included soil, water, and oranges; 152 hand and nasal samples were collected from 76 healthy workers. Antibiotic susceptibility profiles were determined for all staphylococcal isolates. Results: This investigation revealed that of the total Staphylococcus spp. recovered from the orange orchard, 30% (3/10) were resistant to erythromycin, 20% (2/10) were resistant to ampicillin, and 20% (2/10) resistant to both erythromycin and ampicillin. Conclusion: The application of antibiotics to orange trees in open production environments to halt the spread of bacterial disease presents risks to the environment and creates health concerns for Thai farmers using those agents. ARB on crops such as oranges may enter the global food supply and adversely affect public health.

scholarly journals Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure

2021 ◽  
Vol 18 (6) ◽  
pp. 3218
Author(s):  
Ana Rita Almeida ◽  
Marta Tacão ◽  
Joana Soares ◽  
Inês Domingues ◽  
Isabel Henriques
Keyword(s):  
Statistical Significance ◽  
Recovery Period ◽  
Antibiotic Resistance Genes ◽  
Antibiotic Use ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Potential Impact ◽  
Susceptibility Profiles ◽  
Selection Of ◽  
Significant Mortality

The emergence of antibiotic-resistant pathogens due to worldwide antibiotic use is raising concern in several settings, including aquaculture. In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytetracycline (OTC) for two months, followed by a recovery period. The selection of ARB in water and fish was determined using selective media. The abundance of tetA genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control samples, although statistical significance was only achieved five days after exposure. Isolates recovered from samples exposed to the antibiotic were affiliated with Pseudomonas and Stenotrophomonas. Various antibiotic susceptibility profiles were detected and 37% of the isolates displayed multidrug resistance (MDR). The selection of the tetA gene was confirmed by qPCR at the highest OTC concentration tested. Two MDR isolates, tested using zebrafish embryos, caused significant mortality, indicating a potential impact on fish health and survival. Overall, our work highlights the potential impact of antibiotic contamination in the selection of potential pathogenic ARB and ARGS.

scholarly journals Characterization of CRISPR Spacer and Protospacer Sequences in Paenibacillus larvae and Its Bacteriophages

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 459
Author(s):  
Casey Stamereilers ◽  
Simon Wong ◽  
Philippos K. Tsourkas
Keyword(s):  
Comparative Studies ◽  
Point Mutations ◽  
Paenibacillus Larvae ◽  
Bacterial Disease ◽  
American Foulbrood ◽  
Antibiotic Resistant ◽  
Future Studies ◽  
Complete Genomes ◽  
Point Of Reference

The bacterium Paenibacillus larvae is the causative agent of American foulbrood, the most devastating bacterial disease of honeybees. Because P. larvae is antibiotic resistant, phages that infect it are currently used as alternative treatments. However, the acquisition by P. larvae of CRISPR spacer sequences from the phages could be an obstacle to treatment efforts. We searched nine complete genomes of P. larvae strains and identified 714 CRISPR spacer sequences, of which 384 are unique. Of the four epidemiologically important P. larvae strains, three of these have fewer than 20 spacers, while one strain has over 150 spacers. Of the 384 unique spacers, 18 are found as protospacers in the genomes of 49 currently sequenced P. larvae phages. One P. larvae strain does not have any protospacers found in phages, while another has eight. Protospacer distribution in the phages is uneven, with two phages having up to four protospacers, while a third of phages have none. Some phages lack protospacers found in closely related phages due to point mutations, indicating a possible escape mechanism. This study serve a point of reference for future studies on the CRISPR-Cas system in P. larvae as well as for comparative studies of other phage–host systems.

scholarly journals Effect of trickle irrigation on root development of the wet bulb and 'pera' orange tree yield in the state of São Paulo, Brazil

2011 ◽  
Vol 31 (6) ◽  
pp. 1096-1103 ◽  
Author(s):  
Regina C. de M Pires ◽  
Décio Bodine Junior ◽  
Emílio Sakai ◽  
Heloísa L Villar ◽  
Tonny J. A. da Silva ◽  
...  
Keyword(s):  
Root System ◽  
Root Development ◽  
Drip Line ◽  
The State ◽  
Trickle Irrigation ◽  
Yield And Quality ◽  
Orange Orchard ◽  
Orange Trees ◽  
Cleopatra Mandarin

The aim of this study was to evaluate the effect of different microirrigation designs on root system distribution in wet bulb region, orange orchard yield and quality of orange fruits. The experiment was installed as random blocks with five treatments and four replicates in an orchard of 'Pêra' orange trees grafted on 'Cleopatra' mandarin rootstock. The treatments consisted of: one drip line (T1), two drip lines (T2), four drip lines (T3) per planting row, microsprinkler irrigation (T4) and without irrigation (T5). Irrigation treatments favored yield and ºBrix. The treatment with a single drip line (T1) showed the greatest quantity of roots in relation to the treatments T2 and T3.

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

scholarly journals A diversity of Antibiotic-resistant Staphylococcus spp. in a Public Transportation System

2011 ◽  
Vol 2 (3) ◽  
pp. 202-209 ◽  
Author(s):  
Pamela J. Yeh ◽  
Dawn M. Simon ◽  
Jess A. Millar ◽  
H. Forrest Alexander ◽  
Darleen Franklin
Keyword(s):  
Public Transportation ◽  
Transportation System ◽  
Antibiotic Resistant ◽  
Public Transportation System ◽  
Staphylococcus Spp

scholarly journals Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors

2016 ◽  
Vol 113 (29) ◽  
pp. 8188-8193 ◽  
Author(s):  
Alexis I. Cocozaki ◽  
Roger B. Altman ◽  
Jian Huang ◽  
Ed T. Buurman ◽  
Steven L. Kazmirski ◽  
...  
Keyword(s):  
Binding Site ◽  
Single Molecule ◽  
Antibiotic Susceptibility ◽  
Ribosomal Subunit ◽  
Structural Defects ◽  
Resistance Mutations ◽  
Antibiotic Resistant ◽  
E Coli ◽  
X Ray Crystallography ◽  
Susceptibility Profiles

Mutations conferring resistance to translation inhibitors often alter the structure of rRNA. Reduced susceptibility to distinct structural antibiotic classes may, therefore, emerge when a common ribosomal binding site is perturbed, which significantly reduces the clinical utility of these agents. The translation inhibitors negamycin and tetracycline interfere with tRNA binding to the aminoacyl-tRNA site on the small 30S ribosomal subunit. However, two negamycin resistance mutations display unexpected differential antibiotic susceptibility profiles. Mutant U1060A in 16S Escherichia coli rRNA is resistant to both antibiotics, whereas mutant U1052G is simultaneously resistant to negamycin and hypersusceptible to tetracycline. Using a combination of microbiological, biochemical, single-molecule fluorescence transfer experiments, and X-ray crystallography, we define the specific structural defects in the U1052G mutant 70S E. coli ribosome that explain its divergent negamycin and tetracycline susceptibility profiles. Unexpectedly, the U1052G mutant ribosome possesses a second tetracycline binding site that correlates with its hypersusceptibility. The creation of a previously unidentified antibiotic binding site raises the prospect of identifying similar phenomena in antibiotic-resistant pathogens in the future.

scholarly journals Strain diversity and antibiotic-sensitivity of staphylococcus spp. Isolates from patients of multiprofile pediatric hospital in St. Petersburg, Russia

2021 ◽  
Vol 12 (4) ◽  
pp. 15-25
Author(s):  
D. P. Gladin ◽  
A. R. Khairullina ◽  
A. M. Korolyuk ◽  
N. S. Kozlova ◽  
O. V. Ananyeva ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Antibiotic Sensitivity ◽  
Specific Weight ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Therapeutic Effects ◽  
Antimicrobial Drugs ◽  
Antibiotic Resistant ◽  
Resistant Strains ◽  
Staphylococcus Spp

Background. Staphylocci are the leading pus-forming Gram-positive bacteria in the childrens hospitals. The prevalence of the antibiotic resistant strains among them limits therapeutic effects of infections in children. Aim. The research is aimed at characterizing the species of staphylococcus, which are isolated from the different clinical specimens of the patients at the clinics of Saint Petersburg State Pediatric Medical University in 2019, and analysis of their susceptibility to antimicrobial agents. Materials and metods. According to the clinical recommendations of 2018, susceptibility to antimicrobial drugs (AMD) was revealed in 860 strains of staphylococci determined by the disc diffusion method, which were identified by the automated analyser Vitek-2 compact. Results. Six species of staphylococci were represented at the hospital departments, among which Staphylococcus epidermidis prevailed in the departments of the neonate pathology department and intensive care units (63.0% and 46.2% respectively), Staphylococcus aureus is commonly found at the departments of surgery and the departments of the therapeutic profiles (61.7% and 46.2% respectively). More than a half of the staphylococci strains (63.0%) were resistant to at least one of the antimicrobial drugs. Vancomycin and line solid showed the highest activity to these staphylococci. High specific weight of multidrug resistant (MDR) bacteria (37.8%) and extensively drug resistant (XDR) strains of the phenotype (33.0%) was revealed. The level of antibiotic resistant strains was the highest in Staphylococcus haemolyticus (98.1%) and S. epidermidis (82.0%), while the specific weight of the resistant ones, MDR and XDR strains was extremely low among S. aureus (16.2%, 1.5% and 0.4 respectively), as well as in methicillin-resistant isolates (0.8%). Conclusions. A great variety of antibiotic resistance was revealed among the staphylococci. The prevalence of these strains in the pediatric hospitals requires constant local monitoring of the antibiotic resistant staphylococci.

scholarly journals A Retrospective Study of Dogs with Osteomyelitis Secondary to Fractures (2016-2020)

2020 ◽  
Vol 77 (2) ◽  
pp. 65
Author(s):  
Cornel IGNA ◽  
Larisa SCHUSZLER ◽  
Daniel BUMB ◽  
Roxana DASCALU ◽  
Cristian ZAHA ◽  
...  
Keyword(s):  
Healing Time ◽  
Long Bones ◽  
Bone Segment ◽  
Satisfactory Method ◽  
Resistance Rates ◽  
Susceptibility Profiles ◽  
Staphylococcus Spp ◽  
Effective Drugs ◽  
Internal Fracture

The purpose of this work was to evaluate the role of surgical procedures and the effectiveness of antimicrobial therapy in dogs with osteomyelitis secondary to fractures repair.Medical records between 2016 and 2020 were analyzed. Clinical data including signalment, affected bone segment, fracture type, isolated microorganisms, antimicrobial susceptibility profiles, applied therapeutic management and fracture healing time were documented.Eleven cases were confirmed with exogenous osteomyelitis of long bones secondary to fractures repair. The most common microorganisms isolated were Staphylococcus spp., Streptoccocus spp., and Escherichia coli. Amoxicillin and clavulanate potassium followed by ceftriaxone were the most effective drugs. High-resistance rates were documented for penicillin and clindamycin. All 10 cases undergoing surgical revision were cured in 10-17 weeks.Simultaneous debridement with implant removal, reduction, and rigid internal fracture fixation is a satisfactory method for revision surgery in treatment of long bones fractures complicated with osteomyelitis.

scholarly journals A dose response model for quantifying the infection risk of antibiotic-resistant bacteria

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Srikiran Chandrasekaran ◽  
Sunny C. Jiang
Keyword(s):  
Dose Response ◽  
Human Health Risk ◽  
Infection Risk ◽  
Resistant Bacteria ◽  
Microbial Infection ◽  
Total Risk ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Regulatory Policies

AbstractQuantifying the human health risk of microbial infection helps inform regulatory policies concerning pathogens, and the associated public health measures. Estimating the infection risk requires knowledge of the probability of a person being infected by a given quantity of pathogens, and this relationship is modeled using pathogen specific dose response models (DRMs). However, risk quantification for antibiotic-resistant bacteria (ARB) has been hindered by the absence of suitable DRMs for ARB. A new approach to DRMs is introduced to capture ARB and antibiotic-susceptible bacteria (ASB) dynamics as a stochastic simple death (SD) process. By bridging SD with data from bench experiments, we demonstrate methods to (1) account for the effect of antibiotic concentrations and horizontal gene transfer on risk; (2) compute total risk for samples containing multiple bacterial types (e.g., ASB, ARB); and (3) predict if illness is treatable with antibiotics. We present a case study of exposure to a mixed population of Gentamicin-susceptible and resistant Escherichia coli and predict the health outcomes for varying Gentamicin concentrations. Thus, this research establishes a new framework to quantify the risk posed by ARB and antibiotics.

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