Proteomic analysis of the cold stress response in the moss, Physcomitrella patens

Jojoba (Simmondsia chinensis) is a semi-arid, oil-producing industrial crop that have been widely cultivated in tropical arid region. Low temperature is one of the major environmental stress that impair jojoba’s growth, development and yield and limit introduction of jojoba in the vast temperate arid areas. To get insight into the molecular mechanisms of the cold stress response of jojoba, a combined physiological and quantitative proteomic analysis was conducted. Under cold stress, the photosynthesis was repressed, the level of malondialdehyde (MDA), relative electrolyte leakage (REL), soluble sugars, superoxide dismutase (SOD) and phenylalanine ammonia-lyase (PAL) were increased in jojoba leaves. Of the 2821 proteins whose abundance were determined, a total of 109 differentially accumulated proteins (DAPs) were found and quantitative real time PCR (qRT-PCR) analysis of the coding genes for 7 randomly selected DAPs were performed for validation. The identified DAPs were involved in various physiological processes. Functional classification analysis revealed that photosynthesis, adjustment of cytoskeleton and cell wall, lipid metabolism and transport, reactive oxygen species (ROS) scavenging and carbohydrate metabolism were closely associated with the cold stress response. Some cold-induced proteins, such as cold-regulated 47 (COR47), staurosporin and temperature sensitive 3-like a (STT3a), phytyl ester synthase 1 (PES1) and copper/zinc superoxide dismutase 1, might play important roles in cold acclimation in jojoba seedlings. Our work provided important data to understand the plant response to the cold stress in tropical woody crops.

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Cold-Stress Response of Probiotic Lactobacillus plantarum K25 by iTRAQ Proteomic Analysis

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1909.09021 ◽

2020 ◽

Vol 30 (2) ◽

pp. 187-195

Author(s):

Shaoli Liu ◽

Yimiao Ma ◽

Yi Zheng ◽

Wen Zhao ◽

Xiao Zhao ◽

...

Keyword(s):

Stress Response ◽

Cold Stress ◽

Lactobacillus Plantarum ◽

Proteomic Analysis ◽

Probiotic Lactobacillus

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1083 Proteomic analysis reveals increased Nrf2-mediated oxidative stress response in adipose tissue of late pregnant dairy cows during summer heat stress

Journal of Animal Science ◽

10.2527/jam2016-1083 ◽

2016 ◽

Vol 94 (suppl_5) ◽

pp. 519-520

Author(s):

M. Zachut ◽

G. Kra ◽

G. Friedlander ◽

Y. Levin

Keyword(s):

Oxidative Stress ◽

Adipose Tissue ◽

Heat Stress ◽

Stress Response ◽

Dairy Cows ◽

Proteomic Analysis ◽

Oxidative Stress Response ◽

Summer Heat

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GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response

Plant Physiology ◽

10.1093/plphys/kiab142 ◽

2021 ◽

Author(s):

Zemin Wang ◽

Darren Chern Jan Wong ◽

Yi Wang ◽

Guangzhao Xu ◽

Chong Ren ◽

...

Keyword(s):

Stress Response ◽

Cold Stress ◽

Cold Tolerance ◽

Cold Treatment ◽

Ectopic Expression ◽

Bimolecular Fluorescence Complementation ◽

Luciferase Reporter ◽

Vitis Amurensis ◽

Mobility Shift

Abstract Cultivated grapevine (Vitis) is a highly valued horticultural crop, and cold stress affects its growth and productivity. Wild Amur grape (Vitis amurensis) PAT1 (Phytochrome A signal transduction 1, VaPAT1) is induced by low temperature, and ectopic expression of VaPAT1 enhances cold tolerance in Arabidopsis (Arabidopsis thaliana). However, little is known about the molecular mechanism of VaPAT1 during the cold stress response in grapevine. Here, we confirmed the overexpression of VaPAT1 in transformed grape calli enhanced cold tolerance. Yeast two-hybrid and bimolecular fluorescence complementation assays highlighted an interaction between VaPAT1 with INDETERMINATE-DOMAIN 3 (VaIDD3). A role of VaIDD3 in cold tolerance was also indicated. Transcriptome analysis revealed VaPAT1 and VaIDD3 overexpression and cold treatment coordinately modulate the expression of stress-related genes including lipoxygenase 3 (LOX3), a gene encoding a key jasmonate biosynthesis enzyme. Co-expression network analysis indicated LOX3 might be a downstream target of VaPAT1. Both electrophoretic mobility shift and dual luciferase reporter assays showed the VaPAT1-IDD3 complex binds to the IDD-box (AGACAAA) in the VaLOX3 promoter to activate its expression. Overexpression of both VaPAT1 and VaIDD3 increased the transcription of VaLOX3 and JA levels in transgenic grape calli. Conversely, VaPAT1-SRDX (dominant repression) and CRISPR/Cas9-mediated mutagenesis of PAT1-ED causing the loss of the C-terminus in grape calli dramatically prohibited the accumulation of VaLOX3 and JA levels during cold treatment. Together, these findings point to a pivotal role of VaPAT1 in the cold stress response in grape by regulating JA biosynthesis.

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Proteomic analysis of the oxidative stress response inCandida albicans

PROTEOMICS ◽

10.1002/pmic.200600575 ◽

2007 ◽

Vol 7 (5) ◽

pp. 686-697 ◽

Cited By ~ 61

Author(s):

Harald Kusch ◽

Susanne Engelmann ◽

Dirk Albrecht ◽

Joachim Morschhäuser ◽

Michael Hecker

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Proteomic Analysis ◽

Oxidative Stress Response ◽

Incandida Albicans

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Proteomic analysis of Vibrio metschnikovii under cold stress using a quadrupole Orbitrap mass spectrometer

Research in Microbiology ◽

10.1016/j.resmic.2015.07.011 ◽

2015 ◽

Vol 166 (8) ◽

pp. 618-625 ◽

Cited By ~ 5

Author(s):

Juntao Jia ◽

Ying Chen ◽

Yinghui Jiang ◽

Zhengyi Li ◽

Liqing Zhao ◽

...

Keyword(s):

Cold Stress ◽

Mass Spectrometer ◽

Proteomic Analysis ◽

Orbitrap Mass Spectrometer ◽

Vibrio Metschnikovii

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Acute cold stress in rheumatoid arthritis inadequately activates stress responses and induces an increase of interleukin 6

Annals of the Rheumatic Diseases ◽

10.1136/ard.2008.089458 ◽

2008 ◽

Vol 68 (4) ◽

pp. 572-578 ◽

Cited By ~ 16

Author(s):

R H Straub ◽

G Pongratz ◽

H Hirvonen ◽

T Pohjolainen ◽

M Mikkelsson ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Stress Response ◽

Cold Stress ◽

Stress Responses ◽

Plasma Levels ◽

Acute Stress ◽

Whole Body ◽

Cold Therapy ◽

Before And After ◽

Sympathetic Stress

Objective:Acute stress in patients with rheumatoid arthritis (RA) should stimulate a strong stress response. After cryotherapy, we expected to observe an increase of hormones of the adrenal gland and the sympathetic nervous system.Methods:A total of 55 patients with RA were recruited for whole-body cryotherapy at −110°C and −60°C, and local cold therapy between −20°C and −30°C for 7 days. We measured plasma levels of steroid hormones, neuropeptide Y (sympathetic marker), and interleukin (IL)6 daily before and after cryotherapy.Results:In both therapy groups with/without glucocorticoids (GC), hormone and IL6 levels at baseline and 5 h after cold stress did not change over 7 days of cryotherapy. In patients without GC, plasma levels of cortisol and androstenedione were highest after −110°C cold stress followed by −60°C or local cold stress. The opposite was found in patients under GC therapy, in whom, unexpectedly, −110°C cold stress elicited the smallest responses. In patients without GC, adrenal cortisol production increased relative to other adrenal steroids, and again the opposite was seen under GC therapy with a loss of cortisol and an increase of dehydroepiandrosterone. Importantly, there was no sympathetic stress response in both groups. Patients without GC and −110°C cold stress demonstrated higher plasma IL6 compared to the other treatment groups (not observed under GC), but they showed the best clinical response.Conclusions:We detected an inadequate stress response in patients with GC. It is further shown that the sympathetic stress response was inadequate in patients with/without GC. Paradoxically, plasma levels of IL6 increased under strong cold stress in patients without GC. These findings confirm dysfunctional stress axes in RA.

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Cross Talk Between Cold Stress Response Signaling Pathway and Other Stress Response Pathways

Cold Tolerance in Plants ◽

10.1007/978-3-030-01415-5_6 ◽

2018 ◽

pp. 103-109 ◽

Cited By ~ 1

Author(s):

V. C. Dilukshi Fernando

Keyword(s):

Stress Response ◽

Cold Stress ◽

Signaling Pathway ◽

Cross Talk

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Proteogenomic Analysis of Burkholderia Species Strains 25 and 46 Isolated from Uraniferous Soils Reveals Multiple Mechanisms to Cope with Uranium Stress

Cells ◽

10.3390/cells7120269 ◽

2018 ◽

Vol 7 (12) ◽

pp. 269 ◽

Cited By ~ 6

Author(s):

Meenakshi Agarwal ◽

Ashish Pathak ◽

Rajesh Rathore ◽

Om Prakash ◽

Rakesh Singh ◽

...

Keyword(s):

Heavy Metals ◽

Stress Response ◽

Proteomic Analysis ◽

Protein Biosynthesis ◽

Draft Genome ◽

Department Of Energy ◽

Coding Sequences ◽

Total Rna ◽

A Genome ◽

Rna Genes

Two Burkholderia spp. (strains SRS-25 and SRS-46) were isolated from high concentrations of uranium (U) from the U.S. Department of Energy (DOE)-managed Savannah River Site (SRS). SRS contains soil gradients that remain co-contaminated by heavy metals from previous nuclear weapons production activities. Uranium (U) is one of the dominant contaminants within the SRS impacted soils, which can be microbially transformed into less toxic forms. We established microcosms containing strains SRS-25 and SRS-46 spiked with U and evaluated the microbially-mediated depletion with concomitant genomic and proteomic analysis. Both strains showed a rapid depletion of U; draft genome sequences revealed SRS-25 genome to be of approximately 8,152,324 bp, a G + C content of 66.5, containing a total 7604 coding sequences with 77 total RNA genes. Similarly, strain SRS-46 contained a genome size of 8,587,429 bp with a G + C content of 67.1, 7895 coding sequences, with 73 total RNA genes, respectively. An in-depth, genome-wide comparisons between strains 25, 46 and a previously isolated strain from our research (Burkholderia sp. strain SRS-W-2-2016), revealed a common pool of 3128 genes; many were found to be homologues to previously characterized metal resistance genes (e.g., for cadmium, cobalt, and zinc), as well as for transporter, stress/detoxification, cytochromes, and drug resistance functions. Furthermore, proteomic analysis of strains with or without U stress, revealed the increased expression of 34 proteins from strain SRS-25 and 52 proteins from strain SRS-46; similar to the genomic analyses, many of these proteins have previously been shown to function in stress response, DNA repair, protein biosynthesis and metabolism. Overall, this comparative proteogenomics study confirms the repertoire of metabolic and stress response functions likely rendering the ecological competitiveness to the isolated strains for colonization and survival in the heavy metals contaminated SRS soil habitat.

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