Regulation Mechanism of MYC Family Transcription Factors in Jasmonic Acid Signalling Pathway on Taxol Biosynthesis

Paclitaxel is an important anticancer drug. The phytohormone jasmonic acid can significantly induce the biosynthesis of paclitaxel in Taxus, but the molecular mechanism has not yet been resolved. To establish the jasmonic acid signalling pathway of Taxus media, based on the gene of the jasmonic acid signalling pathway of Arabidopsis thaliana, sequence analysis was performed to isolate the jasmonic acid signal from the transcriptome, a transcriptional cluster of pathway gene homologs and the full length of 22 genes were obtained by RACE PCR at 5′ and 3′: two EI ubiquitin ligase genes, COI1-1 and COI1-2;7 MYC bHLH type transcription factor (MYC2, MYC3, MYC4, JAM1, JAM2, EGL3, TT8); 12 JAZ genes containing the ZIM domain; and MED25, one of the components of the transcriptional complex. The protein interaction between each were confirmed by yeast two hybridization and bimolecular fluorescence complementation based on similar genes interaction in Arabidopsis. A similar jasmonate signaling pathway was illustrated in T. media. All known paclitaxel biosynthesis genes promoters were isolated by genome walker PCR. To investigate the jasmonate signaling effect on these genes’ expression, the transcription activity of MYC2, MYC3 and MYC4 on these promoters were examined. There are 12, 10 and 11 paclitaxel biosynthesis genes promoters that could be activated by MYC2, MYC3 and MYC4.

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Broad‐spectrum stress tolerance conferred by suppressing jasmonate signaling attenuation in Arabidopsis JASMONIC ACID OXIDASE (JAO) mutants

The Plant Journal ◽

10.1111/tpj.15598 ◽

2021 ◽

Author(s):

Valentin Marquis ◽

Ekaterina Smirnova ◽

Stéfanie Graindorge ◽

Pauline Delcros ◽

Claire Villette ◽

...

Keyword(s):

Jasmonic Acid ◽

Stress Tolerance ◽

Broad Spectrum ◽

Acid Oxidase ◽

Jasmonate Signaling

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Are wild insectivorous birds attracted to methyl-jasmonate-treated Pyrenean oak trees?

Behaviour ◽

10.1163/1568539x-00003516 ◽

2018 ◽

Vol 155 (13-15) ◽

pp. 945-967 ◽

Cited By ~ 1

Author(s):

Irene Saavedra ◽

Luisa Amo

Keyword(s):

Jasmonic Acid ◽

Methyl Jasmonate ◽

Field Experiments ◽

Predation Rate ◽

Signalling Pathway ◽

Insectivorous Birds ◽

Oak Trees ◽

Prey Location ◽

Oak Tree ◽

Herbivore Induced Plant Volatiles

Abstract Plants emit herbivore-induced plant volatiles (HIPVs) when they are attacked by herbivorous arthropods to attract natural enemies of the herbivores. Previous studies have suggested that insectivorous birds can detect the HIPVs for prey location. The production of HIPVs is partly mediated by the jasmonic acid signalling pathway. Methyl jasmonate is one compound involved in the jasmonic acid signalling pathway. Therefore, spraying of methyl jasmonate (MeJa) solution on plants can induce the emission of volatiles similar to the HIPVs induced by herbivory. We performed two field experiments to test whether insectivorous adult birds are attracted to Pyrenean oak trees (Quercus pyrenaica) treated with MeJa solutions. We used artificial larvae to measure bird predation rate. There were no differences in the predation rates of plasticine larvae between control and MeJa treatments, suggesting that insectivorous birds were not attracted to trees treated with MeJa. We also tested whether blue tit nestlings without previous experience in foraging respond to the scent of Pyrenean oak tree leaves treated with MeJa, to test whether the attraction to MeJa-treated trees is innate. A similar number of nestlings performed begging responses when exposed to the scent of leaves of MeJa-treated trees compared to the scent of leaves of control untreated trees. Furthermore, the duration of the first begging was similar in response to the volatiles emitted by MeJa-treated trees and untreated trees. Despite previous evidence suggesting that insectivorous birds are attracted to HIPVs, our results show that neither insectivorous adult birds nor inexperienced nestlings were attracted to trees treated with MeJa. Further studies are needed to disentangle whether the differences in the emission of volatiles between herbivore-infested trees and MeJa-treated trees can explain this lack of attraction to MeJa-treated Pyrenean oak trees.

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Risk of Wnt/β-catenin signalling pathway gene polymorphisms in primary Sjögren’s syndrome

Rheumatology ◽

10.1093/rheumatology/kez269 ◽

2019 ◽

Vol 59 (2) ◽

pp. 418-425 ◽

Cited By ~ 1

Author(s):

Javier Fernández-Torres ◽

Nonanzit Pérez-Hernández ◽

Gabriela Hernández-Molina ◽

Gabriela A Martínez-Nava ◽

Daniela Garrido-Rodríguez ◽

...

Keyword(s):

Dimensionality Reduction ◽

Multifactor Dimensionality Reduction ◽

Gene Interaction ◽

Signalling Pathway ◽

Multifactor Dimensionality Reduction Analysis ◽

Exact Test ◽

Risk Alleles ◽

Pathway Gene ◽

Multifactor Dimensionality Reduction Method ◽

Dimensionality Reduction Method

Abstract Objective To explore genetic polymorphisms of the Wnt/β-catenin signalling pathway in primary SS (PSS). Methods We included 98 patients with PSS and 165 healthy volunteers. Genomic DNA was extracted from peripheral blood samples. Through an open-array platform of low density, we genotyped 25 polymorphisms from 14 genes (WISP1, DKK1, SOST, FRZB, LRP1, LRP4, LRP5, LRP6, GSKB, ADAMTS5, GDF5, FMN2, ADIPOQ and COL11A1) involved in the Wnt/β-catenin signalling pathway. We compared the allelic and genotypic frequencies with Fisher’s exact test and logistic regression analysis adjusted by age, gender and individual admixture, as well as bootstrap-resampling analysis. We assessed the gene–gene interaction by the multifactor dimensionality reduction method. Results We found a positive significant association with four polymorphisms: LRP5 rs606989, FRZB rs409238, GSK3B rs2037547 and ADIPOQ rs2241766. All of them conferred risk for PSS, being the highest among subjects carrying three to four risk alleles (P < 0.001). According to a multifactor dimensionality reduction analysis, the best models included the LRP5 (rs606989), FRZB (rs409238) and ADIPOQ (rs2241766) polymorphisms. Conclusion LRP5, FRZB and ADIPOQ genes related in the Wnt/β-catenin signalling pathway increased the risk of PSS. Further research is needed to establish their functional role in this clinical entity.

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Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula × Tina

Journal of Experimental Botany ◽

10.1093/jxb/eraa159 ◽

2020 ◽

Vol 71 (12) ◽

pp. 3749-3758 ◽

Cited By ~ 1

Author(s):

Ondřej Kocáb ◽

Jana Jakšová ◽

Ondřej Novák ◽

Ivan Petřík ◽

René Lenobel ◽

...

Keyword(s):

Jasmonic Acid ◽

Enzyme Activities ◽

Digestive Enzymes ◽

Defense Mechanisms ◽

Prey Capture ◽

Digestive Enzyme ◽

Carnivorous Plants ◽

Alpha Amylase ◽

Jasmonate Signaling ◽

Digestive Enzyme Activities

Abstract Carnivorous plants within the order Caryophyllales use jasmonates, a class of phytohormone, in the regulation of digestive enzyme activities. We used the carnivorous butterwort Pinguicula × Tina from the order Lamiales to investigate whether jasmonate signaling is a universal and ubiquitous signaling pathway that exists outside the order Caryophyllales. We measured the electrical signals, enzyme activities, and phytohormone tissue levels in response to prey capture. Mass spectrometry was used to identify proteins in the digestive secretion. We identified eight enzymes in the digestive secretion, many of which were previously found in other genera of carnivorous plants. Among them, alpha-amylase is unique in carnivorous plants. Enzymatic activities increased in response to prey capture; however, the tissue content of jasmonic acid and its isoleucine conjugate remained rather low in contrast to the jasmonate response to wounding. Enzyme activities did not increase in response to the exogenous application of jasmonic acid or coronatine. Whereas similar digestive enzymes were co-opted from plant defense mechanisms among carnivorous plants, the mode of their regulation differs. The butterwort has not co-opted jasmonate signaling for the induction of enzyme activities in response to prey capture. Moreover, the presence of alpha-amylase in digestive fluid of P. × Tina, which has not been found in other genera of carnivorous plants, might indicate that non-defense-related genes have also been co-opted for carnivory.

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The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco

Frontiers in Plant Science ◽

10.3389/fpls.2021.810990 ◽

2022 ◽

Vol 12 ◽

Author(s):

Xiaokun Liu ◽

Jingjing Duan ◽

Dan Huo ◽

Qinqin Li ◽

Qiaoyun Wang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Bimolecular Fluorescence Complementation ◽

Luciferase Reporter ◽

Myb Transcription Factor ◽

Anthocyanin Synthesis ◽

Regulation Mechanism ◽

Reporter Assay ◽

Anthocyanin Accumulation ◽

Tree Peony ◽

R2r3 Myb

Paeonia qiui is a wild species of tree peony native to China. Its leaves are purplish red from the bud germination to the flowering stage, and anthocyanin is the main pigment in purplish red leaves. However, the anthocyanin synthesis regulation mechanism in tree peony leaves remains unclear. In this study, an R2R3-MYB, PqMYB113 was identified from the leaves of P. qiui. Phylogenetic analysis revealed that PqMYB113 clustered with Liquidambar LfMYB113 and grape VvMYBA6. Subcellular location analysis showed that PqMYB113 was located in the cell nucleus. The transient reporter assay suggested that PqMYB113 was a transcriptional activator. The overexpression of PqMYB113 in Arabidopsis thaliana and tobacco (Nicotiana tabacum) resulted in increased anthocyanin accumulation and the upregulation of CHS, F3H, F3’H, DFR, and ANS. The dual luciferase reporter assay showed that PqMYB113 could activate the promoters of PqDFR and PqANS. Bimolecular fluorescence complementation assays and yeast two-hybrid assays suggested that PqMYB113 could form a ternary MBW complex with PqbHLH1 and PqWD40 cofactors. These results provide insight into the regulation of anthocyanin biosynthesis in tree peony leaves.

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Cloning and Characterization of an Agglutinin Gene from Arisaema lobatum

Bioscience Reports ◽

10.1007/s10540-005-2895-4 ◽

2005 ◽

Vol 25 (5-6) ◽

pp. 345-362 ◽

Cited By ~ 4

Author(s):

Juan Lin ◽

Xuanwei Zhou ◽

Yongzhen Pang ◽

Han Gao ◽

Jiong Fei ◽

...

Keyword(s):

Genomic Sequence ◽

Three Dimensional ◽

Dimensional Structure ◽

Regulation Mechanism ◽

Amino Acid Residues ◽

Reading Frame ◽

Mannose Binding ◽

Pcr Technology ◽

Plant Families ◽

Race Pcr

A novel agglutinin gene was cloned from Arisaema lobatum using SMART RACE-PCR technology. The full-length cDNA of Arisaema lobatum agglutinin (ala) was 1078 bp and contained a 774 bp open reading frame encoding a lectin precursor (proproprotein) of 258 amino acid residues with a 23 aa signal peptide. ALA contained three mannose-binding sites (QXDXNXVXY) with two-conserved domains of 45% identity, ALA-DOM1 and ALA-DOM2. The three-dimensional structure of ALA was very similar to that of GNA (Galanthus nivalis agglutinin). ALA shared varying identities, ranging from 40% to 85%, with mannose-binding lectins from other species of plant families, such as Araceae, Alliaceae, Iridaceae, Lillaceae, Amaryllidaceae and Bromeliaceae. Genomic sequence of ala was also cloned using genomic walker technology, and it was found to contain three putative TATA boxes and eight possible CAAT boxes in the 5′-flanking region. No intron was found within the region of genomic sequence. Southern blot analysis indicated that the ala belonged to a multi-copy gene family. Expression pattern analysis revealed that the ala preferentially expressed in the tissues with the higher expression being found in spadix, bud, leaf, spathe and tuber. The cloning of the ala gene not only provides a basis for further investigation of its structure, expression and regulation mechanism, but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into plants in the future.

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Cytokinin promotes jasmonic acid accumulation in the control of maize leaf growth

10.1101/760405 ◽

2019 ◽

Author(s):

Aimee N. Uyehara ◽

Angel R. Del Valle-Echevarria ◽

Charles T. Hunter ◽

Hilde Nelissen ◽

Kirin Demuynck ◽

...

Keyword(s):

Growth Rate ◽

Cell Division ◽

Jasmonic Acid ◽

Leaf Growth ◽

Leaf Size ◽

Wild Type ◽

Cytokinin Signaling ◽

Transcript Accumulation ◽

Maize Leaf ◽

Pathway Gene

AbstractGrowth of plant organs results from the combined activity of cell division and cell expansion. The coordination of these two processes depends on the interplay between multiple hormones that determine final organ size. Using the semidominant Hairy Sheath Frayed1 (Hsf1) maize mutant, that hypersignals the perception of cytokinin (CK), we show that CK can reduce leaf size and growth rate by decreasing cell division. Linked to CK hypersignaling, the Hsf1 mutant has increased jasmonic acid (JA) content, a hormone that can inhibit cell division. Treatment of wild type seedlings with exogenous JA reduces maize leaf size and growth rate, while JA deficient maize mutants have increased leaf size and growth rate. Expression analysis revealed increased transcript accumulation of several JA pathway genes in the Hsf1 leaf growth zone. A transient treatment of growing wild type maize shoots with exogenous CK also induced JA pathway gene expression, although this effect was blocked by co-treatment with cycloheximide. Together our results suggest that CK can promote JA accumulation possibly through increased expression of specific JA pathway genes.One sentence summaryCytokinin-signaling upregulates the jasmonate biosynthesis pathway, resulting in jasmonate accumulation and influences on maize leaf growth.

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The rice SCFOsFBK1 E3 ligase mediates jasmonic acid induced degradation of a RING-H2 protein and the cinnamoyl-CoA reductase, OsCCR14

10.21203/rs.3.rs-300884/v1 ◽

2021 ◽

Author(s):

Pratikshya Borah ◽

Aishwarye Sharma ◽

Jitendra Khurana

Keyword(s):

Jasmonic Acid ◽

Conformational Changes ◽

Molecular Level ◽

Nuclear Periphery ◽

E3 Ligase ◽

Bimolecular Fluorescence Complementation ◽

Stable Complex ◽

Cytoplasmic Localization ◽

Cinnamoyl Coa Reductase ◽

Coa Reductase

Abstract We had previously shown the rice F-box, OsFBK1, plays a role in anther development by mediating the turnover of OsCCR14, a cinnamoyl CoA-reductase regulating lignification. Another substrate identified in the same Y2H library screening was OsATL53, a member of the ATL family of RING-H2 proteins that is primarily localized to the cytoplasm. We found OsATL53 to be a component and substrate of SCFOsFBK1 by immunoprecipitation and cell-free studies. Incidentally, OsATL53 was found to interact with OsCCR14 in the cytoplasm and form a stable complex in cell-free experiments and bimolecular fluorescence complementation assays. Biochemically, OsATL53 was found to influence the enzymatic activity of OsCCR14 by decreasing its efficiency. Degradation studies have shown OsFBK1 mediates turnover of OsCCR14 in the nucleus, while OsATL53 is degraded in both cytoplasm and nucleus. The degradation of ATLs by F-box proteins has not been reported before. In presence of jasmonic acid (JA), which plays a role in anther dehiscence, OsATL53 has been found to gather around the nucleus, and this property enables the translocation of the OsATL53-OsCCR14 complex from a cytoplasmic localization to accumulate around the nuclear periphery. FLIM analyses revealed OsCCR14-OsATL53 complex undergoing conformational changes in presence of JA and this triggers OsFBK1 to mediate the targeted degradation of OsATL53 in the cytoplasm, thereby dissociating the cytoplasmic OsCCR14-OsATL53 complex and enabling OsCCR14 to enter the nucleus and eventually get degraded by SCFOsFBK1 E3 ligase. We have thus studied the signalling mechanism of a variant JA-induced E3 ligase-mediated substrate turnover in plants at the molecular level.

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Interactions of JAZ Repressors with Anthocyanin Biosynthesis-Related Transcription Factors of Fragaria × ananassa

Agronomy ◽

10.3390/agronomy10101586 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1586

Author(s):

Adrián Garrido-Bigotes ◽

Marcela Torrejón ◽

Roberto Solano ◽

Carlos R. Figueroa

Keyword(s):

Transcription Factors ◽

Protein Interactions ◽

Anthocyanin Biosynthesis ◽

Phylogenetic Analyses ◽

Molecular Targets ◽

Fragaria Ananassa ◽

Jasmonate Signaling ◽

High Conservation ◽

Transcriptional Complex ◽

Signaling Components

Strawberry fruits are rich in flavonoids like proanthocyanidins and anthocyanins. Their biosynthesis and accumulation are controlled by the MYB-bHLH-WD40 (MBW) transcriptional complex, which is mainly formed by basic helix-loop-helix (bHLH) and MYB transcription factors (TFs). In Arabidopsis thaliana both bHLH and MYB TFs are repressed by JASMONATE ZIM-DOMAIN (JAZ) proteins, the key repressors of the jasmonate-signaling pathway. The aim of this research was the characterization of the FaJAZ1/8.1/9/10 proteins and molecular targets of signaling components and anthocyanin biosynthesis-related TFs of Fragaria × ananassa by protein–protein interactions. For this, domain compositions were studied by multiple alignments and phylogenetic analyses, while interactions were analyzed by yeast two-hybrid (Y2H) assays. We detected high conservation of FaJAZ proteins and jasmonate-signaling components, as well as FabHLHs and FaMYB10 TFs. Moreover, we report the F. × ananassa YABBY1 (FaYAB1) TF, which is related to anthocyanin biosynthesis in Arabidopsis, showed high conservation of functional domains. We demonstrated that FaJAZ repressors interacted with F. × ananassa NOVEL INTERACTOR OF JAZ (FaNINJA), FaMYC2, and JASMONATE ASSOCIATED MYC2-LIKE (FaJAM) proteins. Besides, transcription factors of MBW-complex like FabHLH3, FabHLH33, and FaMYB10, together with FaYAB1, were molecular targets of FaJAZ repressors, exhibiting specificity or redundancy of interaction depending on particular FaJAZ protein. Overall, these results suggest that interactions of jasmonate-signaling components are fully conserved, and anthocyanin biosynthesis might be regulated by JAZ repressors in F. × ananassa.

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