scholarly journals Nitrogen Fertilizer Induced Alterations in The Root Proteome of Two Rice Cultivars

2019 ◽  
Vol 20 (15) ◽  
pp. 3674 ◽  
Author(s):  
Tang ◽  
Sun ◽  
Chen ◽  
Damaris ◽  
Lu ◽  
...  
Keyword(s):  
Crop Production ◽  
Oryza Sativa L ◽  
N Fertilizer ◽  
Differentially Expressed ◽  
Growth And Yield ◽  
Limiting Factor ◽  
Fertilizer Treatment ◽  
Rice Cultivars ◽  
Differentially Expressed Proteins ◽  
Proteomic Approach

Nitrogen (N) is an essential nutrient for plants and a key limiting factor of crop production. However, excessive application of N fertilizers and the low nitrogen use efficiency (NUE) have brought in severe damage to the environment. Therefore, improving NUE is urgent and critical for the reductions of N fertilizer pollution and production cost. In the present study, we investigated the effects of N nutrition on the growth and yield of the two rice (Oryza sativa L.) cultivars, conventional rice Huanghuazhan and indica hybrid rice Quanliangyou 681, which were grown at three levels of N fertilizer (including 135, 180 and 225 kg/hm2, labeled as N9, N12, N15, respectively). Then, a proteomic approach was employed in the roots of the two rice cultivars treated with N fertilizer at the level of N15. A total of 6728 proteins were identified, among which 6093 proteins were quantified, and 511 differentially expressed proteins were found in the two rice cultivars after N fertilizer treatment. These differentially expressed proteins were mainly involved in ammonium assimilation, amino acid metabolism, carbohydrate metabolism, lipid metabolism, signal transduction, energy production/regulation, material transport, and stress/defense response. Together, this study provides new insights into the regulatory mechanism of nitrogen fertilization in cereal crops.

Clomazone and Starter Nitrogen Fertilizer Effects on Growth and Yield of Hybrid and Inbred Rice Cultivars

2017 ◽  
Vol 31 (2) ◽  
pp. 207-216 ◽  
Author(s):  
Bobby R. Golden ◽  
Benjamin H. Lawrence ◽  
Jason A. Bond ◽  
H. Matthew Edwards ◽  
Timothy W. Walker
Keyword(s):  
Rice Yield ◽  
Leaf Growth ◽  
Differential Susceptibility ◽  
Field Studies ◽  
N Fertilizer ◽  
Growth And Yield ◽  
Fertilizer Treatment ◽  
Rice Cultivars ◽  
Early Season ◽  
Rough Rice

Cultivar and/or application of early-season (starter) nitrogen (N) fertilizer may influence rice tolerance to clomazone. Field studies were conducted to compare the response of hybrid and inbred rice cultivars to applications of clomazone and starter N fertilizer treatments. The inbred cultivar ‘Cocodrie’ and the hybrid cultivar ‘XL723’ were treated with clomazone at 0, 420, or 672 g ai ha−1immediately after seeding, and starter N fertilizer was applied at 0 or 24 kg N ha−1when rice reached the two-leaf growth stage. Pooled across clomazone rates and starter N fertilizer treatments, height of Cocodrie 1 week after emergence (WAE) was greater than that of XL723 in 1 of 3 yr. The difference in height between Cocodrie and XL723 resulted from greater clomazone injury 1 WAE on XL723 compared with Cocodrie. No differences in rice height 3 WAE were detected between Cocodrie and XL723 in 2 of 3 yr. when data were pooled across clomazone rates and starter N fertilizer treatments. Injury 3 WAE was similar for Cocodrie across the 3 yr., but injury on XL723 was greater in 1 of 3 yr. Rough rice yield was lower in plots treated with either rate of clomazone where no starter N fertilizer treatment was applied; however, in plots receiving a starter N fertilizer treatment, no effect of clomazone rate on rough rice yield was observed. Clomazone rate did not influence rough rice yield of Cocodrie in any single yr., but rough rice yields of XL723 were lower in plots receiving clomazone compared with plots that received no clomazone in 1 of 3 yr. Therefore, differential susceptibility to clomazone between Cocodrie and XL723 exists based on early-season response and rough rice yield. Starter N fertilizer treatments were beneficial for overcoming yield reductions due to clomazone injury.

scholarly journals Analysis of Proteomic Profile of Contrasting Phosphorus Responsive Rice Cultivars Grown under Phosphorus Deficiency

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1028
Author(s):  
Aadil Yousuf Tantray ◽  
Hayssam M. Ali ◽  
Altaf Ahmad
Keyword(s):  
Oryza Sativa L ◽  
Phosphorus Deficiency ◽  
Expression Patterns ◽  
Phosphorus Uptake ◽  
Crop Productivity ◽  
Differentially Expressed ◽  
Limiting Factors ◽  
Rice Cultivars ◽  
Differentially Expressed Proteins ◽  
P Deficiency

Phosphorus (P) deficiency is one of the major limiting factors for crop productivity. The yield of rice (Oryza sativa L.) is severely limited by phosphorus deficiency. An attempt has been made in this study to identify P deficiency responsive differentially expressed proteins of rice through analysis of leaf proteome of contrasting P-responsive rice cultivars under P deficiency conditions because genetic variability has been found in the rice cultivars for adaptive response to P deficiency and a controlled regulatory system is involved in the P deficiency adaptation response. Phosphorus-efficient (cv. Panvel) and P-inefficient (cv. Nagina 22) rice cultivars were hydroponically grown in the nutrient medium under control environmental conditions at low-P level (2.0 µM) and optimum-P level (320 µM) treatments. Expression patterns of the proteins of the leaves of both the cultivars were analyzed in 30-day-old plants. The identification of these proteins through mass spectrometry and MASCOT software (Matrix Science Inc., Boston, USA) revealed that these differentially expressed proteins were homologous to known functional proteins involved in energy metabolism, biosynthesis, photosynthesis, signaling, protein synthesis, protein folding, phospholipid metabolism, oxidative stress, transcription factors, and phosphorus metabolism. It has been observed that rice cultivars responded differently to low-P treatment through modification in protein expressions pattern to maintain the growth of the plants. Therefore, the expression patterns of proteins were different in both of the cultivars under low-P treatment. Higher potential of protein stability, stress tolerance, osmo-protection, and regulation of phosphorus uptake was observed in cv. Panvel than cv. Nagina 22. This study could help to unravel the complex regulatory process that is involved in adaptation to P deficiency in rice.

Identification of differentially expressed proteins in retinoblastoma tumors using mass spectrometry-based comparative proteomic approach

2017 ◽  
Vol 159 ◽  
pp. 77-91 ◽  
Author(s):  
Jasmine Naru ◽  
Ritu Aggarwal ◽  
Ashok Kumar Mohanty ◽  
Usha Singh ◽  
Deepak Bansal ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Proteomic Approach

Proteomic analysis of the effects of lutein on mammary gland metabolism in dairy cows

2018 ◽  
Vol 85 (2) ◽  
pp. 152-156
Author(s):  
Caihong Wang ◽  
Chong Wang ◽  
Jianxin Liu ◽  
Hongyun Liu
Keyword(s):  
Blood Flow ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Control Diet ◽  
Differentially Expressed ◽  
Acid Oxidation ◽  
Differentially Expressed Proteins ◽  
Proteomic Approach ◽  
Increased Blood Flow

The aim of the research reported in this Research Communication was to identify differentially expressed proteins in dairy cows with normal and lutein diet and to elucidate the mechanisms of lutein-induced effects on bovine mammary gland metabolism using a comparative proteomic approach. Thirty-three differentially expressed proteins were identified from mammary gland of control diet-fed and lutein diet-fed dairy cows. Among these proteins, 15 were upregulated and 18 were downregulated in the lutein group. Functional analysis of the differentially expressed proteins showed that increased blood flow, depressed glycolysis, enhanced lactose anabolism, decreased fatty acid oxidation and up-regulated beta lactoglobulin expression were connected with lutein addition. These results suggested that the increased blood flow, reduced glucose catabolism, enhanced capacity for milk lactose synthesis, depressed fatty acid catabolism and increased expression of antioxidantion related protein may be the prime factors contributing to the increased milk production and enhanced immune status in lutein-fed dairy cows. This study provides molecular mechanism of dietary lutein in regulating lactation of dairy cows.

scholarly journals Proteomic profiling reveals differentially expressed proteins associated with amylose accumulation during rice grain filling

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Hengdong Zhang ◽  
Jiana Chen ◽  
Shuanglü Shan ◽  
Fangbo Cao ◽  
Guanghui Chen ◽  
...  
Keyword(s):  
Amylose Content ◽  
Starch Synthesis ◽  
Grain Filling ◽  
Adenosine Diphosphate ◽  
Differentially Expressed ◽  
Rice Grain ◽  
Rice Cultivars ◽  
Differentially Expressed Proteins ◽  
Proteomic Profiling ◽  
Rice Grains

Abstract Background Amylose accumulation in rice grains is controlled by genetic and environmental factors. Amylose content is a determinant factor of rice quality in terms of cooking and eating. Great variations in amylose content in indica rice cultivars have been observed. The current study was to identify differentially expressed proteins in starch and sucrose metabolism and glycolysis/gluconeogenesis pathways and their relationships to amylose synthesis using two rice cultivars possess contrasting phenotypes in grain amylose content. Results Synthesis and accumulation of amylose in rice grains significantly affected the variations between rice cultivars in amylose contents. The high amylose content cultivar has three down-regulated differentially expressed proteins, i.e., LOC_Os01g62420.1, LOC_Os02g36600.1, and LOC_Os08g37380.2 in the glycolysis/gluconeogenesis pathway, which limit the glycolytic process and decrease the glucose-1-phosphate consumption. In the starch and sucrose metabolic pathway, an up-regulated protein, i.e., LOC_Os06g04200.1 and two down-regulated proteins, i.e., LOC_Os05g32710.1 and LOC_Os04g43360.1 were identified (Figure 4). Glucose-1-phosphate is one of the first substrates in starch synthesis and glycolysis that are catalyzed to form adenosine diphosphate glucose (ADPG), then the ADPG is catalyzed by granule-bound starch synthase I (GBSS I) to elongate amylose. Conclusions The results indicate that decreasing the consumption of glucose-1-phosphate in the glycolytic process is essential for the formation of ADPG and UDPG, which are substrates for amylose synthesis. In theory, amylose content in rice can be regulated by controlling the fate of glucose-1-phosphate.

N Fertilizer Dose-Dependent Efficiency of Serratia spp. for Improving Growth and Yield of Upland Rice (Oryza sativa L.)

2019 ◽  
Vol 13 (3) ◽  
pp. 217-226 ◽  
Author(s):  
Adriano Stephan Nascente ◽  
Anna Cristina Lanna ◽  
Thatyane Pereira de Sousa ◽  
Amanda Abdallah Chaibub ◽  
Alan Carlos Alves de Souza ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Upland Rice ◽  
Oryza Sativa L ◽  
N Fertilizer ◽  
Growth And Yield ◽  
Dose Dependent

scholarly journals iTRAQ Quantitative Analysis of Multidrug Resistance Mechanisms in Human Gastric Cancer Cells

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Huai-Dong Hu ◽  
Feng Ye ◽  
Da-Zhi Zhang ◽  
Peng Hu ◽  
Hong Ren ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Multidrug Resistance ◽  
Cell Line ◽  
Molecular Mechanisms ◽  
Absolute Quantification ◽  
Differentially Expressed ◽  
Human Gastric Cancer ◽  
Differentially Expressed Proteins ◽  
Gastric Cancer Cells ◽  
Proteomic Approach

Multidrug resistance (MDR) is a major obstacle towards a successful treatment of gastric cancer. However, the mechanisms of MDR are intricate and have not been fully understood. To elucidate the molecular mechanisms of MDR in gastric cancer, we employed the proteomic approach of isobaric tags for relative and absolute quantification (iTRAQ), followed by LC-MS/MS, using the vincristine-resistant SGC7901/VCR cell line and its parental SGC7901 cell line as a model. In total, 820 unique proteins were identified and 91 proteins showed to be differentially expressed in SGC7901/VCR compared with SGC7901. Several differentially expressed proteins were further validated by western blot analysis. Furthermore, the association of MVP, one of the highly expressed proteins in SGC7901/VCR, with MDR was verified. Our study is the first application of iTRAQ technology for MDR mechanisms analysis in gastric cancer, and many of the differentially expressed proteins identified have not been linked to MDR in gastric cancer before, which showed the value of this technology in identifying differentially expressed proteins in cancer.

Proteomic analysis of differentially expressed proteins in the roots of Columbia-0 and Landsberg erecta ecotypes of Arabidopsis thaliana in response to aluminum toxicity

2012 ◽  
Vol 92 (7) ◽  
pp. 1267-1282 ◽  
Author(s):  
T. Karuppanapandian ◽  
S-J. Rhee ◽  
E-J. Kim ◽  
B. K. Han ◽  
O. A. Hoekenga ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Stress Responses ◽  
Proteomic Analysis ◽  
Crop Production ◽  
Aluminum Toxicity ◽  
Expression Patterns ◽  
Differentially Expressed ◽  
Monodehydroascorbate Reductase ◽  
Differentially Expressed Proteins ◽  
Al Stress

Karuppanapandian, T., Rhee, S.-J., Kim, E.-J., Han, B. K., Hoekenga, O. A. and Lee, G. P. 2012. Proteomic analysis of differentially expressed proteins in the roots of Columbia-0 and Landsberg erecta ecotypes of Arabidopsis thaliana in response to aluminum-toxicity. Can. J. Plant Sci. 92: 1267–1282. Aluminum (Al) is phytotoxic when solubilized into Al3+ in acidic soils and represents a major constraint for crop production. The present study describes Al-stress responses in roots of Al-tolerant and Al-sensitive Arabidopsis ecotypes, Columbia-0 (Col-0) and Landsberg erecta (Ler), respectively. Comparative proteomic analysis was applied to plants grown in hydroponic solution culture under acidic pH (4.2) conditions. To investigate time-dependent responses, 6-d-old seedlings were treated with 30 µM AlCl3 for 24, 48, or 72 h; total proteins were prepared from roots and separated by two-dimensional gel electrophoresis (2-DE). From 2-DE analysis, were 600 proteins were inspected, 29 proteins were differentially responsive to Al-treatment. The 2-DE patterns were compared and differentially expressed proteins identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Analysis of protein expression patterns revealed that a set of proteins is functionally associated with tricarboxylic acid (TCA) cycle and glycolysis, reactive oxygen quenching and detoxification mechanism, and signal transduction pathways, etc., could play important roles in mediating plant response to Al in Arabidopsis ecotypes. Comparison of the changes in the protein profiles revealed that Al-stress increased Al-tolerance related proteins in Al-tolerant Col-0, but only generic stress responses occurred in Al-sensitive Ler. Specifically, Al up-regulated proteins such as alcohol dehydrogenase, monodehydroascorbate reductase, GTP-binding nuclear protein Ran-2, and leucine aminopeptidase in Col-0 but not in Ler.

scholarly journals Application of local microbes increases growth and yield of several local upland rice cultivars of Southeast Sulawesi, Indonesia

2022 ◽  
Vol 951 (1) ◽  
pp. 012011
Author(s):  
T Wijayanto ◽  
D Boer ◽  
A Aco ◽  
N Mu’min ◽  
A Khaeruni ◽  
...  
Keyword(s):  
Crop Production ◽  
Upland Rice ◽  
Rice Cultivar ◽  
Biological Agents ◽  
Research Field ◽  
Rice Production ◽  
Growth And Yield ◽  
Rice Cultivars ◽  
Dry Land ◽  
Food Needs

Abstract Rice is the major food commodity in Indonesia and many other countries, as the main source of carbohydrate. Rice production must be increased continuously to meet food needs, one of which is by utilizing largely available dry land areas. Two important factors required to increase rice production on marginal soils are the use of high-production adaptive varieties and biological agents. It is necessary to conduct research on the application of local microbes to the cultivation of upland rice cultivars, with the aim to determine the best genotypes and/or suitable microbes. This research was carried out at the Research Field and laboratories of Faculty of Agriculture, University of Halu Oleo, from April 2020 to April 2021. The first tested factor was biological agents (local microbes), consisting of three levels, namely: without microbes (M0), the fungus Trichoderma sp. (M1), and the bacteria Pseudomonas sp. (M2). The second factor was the upland rice cultivar, which consisted of five levels, namely: V1 (Tinangge cultivar), V2 (Enggalaru cultivar), V3 (Bakala cultivar), V4 (Momea cultivar), and 1 nasional variety, namely V5 (Inpago-12 variety), as check variety. The research results showed that the interaction between local microbes Trichoderma sp. (M1) and Pseudomonas sp. (M2) on Tinangge (V1) and Momea (V4) cultivars gave a better effect on crop production. The best cultivar based on the growth and yield variables was generally obtained from the Momea cultivar (V4), although in many variables it was not significantly different from the Tinangge cultivar (V1). These cultivars could be further studied and possibly developed for promising cultivars in Kendari areas.

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