scholarly journals Combined Effect of Cadmium and Lead on Durum Wheat

2019 ◽  
Vol 20 (23) ◽  
pp. 5891 ◽  
Author(s):  
Alessio Aprile ◽  
Erika Sabella ◽  
Enrico Francia ◽  
Justyna Milc ◽  
Domenico Ronga ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Toxic Metals ◽  
Molecular Mechanisms ◽  
Field Studies ◽  
Combined Effect ◽  
Toxic Heavy Metals ◽  
Wheat Roots ◽  
Metal Transporters ◽  
Hydroponic Experiment ◽  
Cadmium And Lead

Cadmium (Cd) and lead (Pb) are two toxic heavy metals (HMs) whose presence in soil is generally low. However, industrial and agricultural activities in recent years have significantly raised their levels, causing progressive accumulations in plant edible tissues, and stimulating research in this field. Studies on toxic metals are commonly focused on a single metal, but toxic metals occur simultaneously. The understanding of the mechanisms of interaction between HMs during uptake is important to design agronomic or genetic strategies to limit contamination of crops. To study the single and combined effect of Cd and Pb on durum wheat, a hydroponic experiment was established to examine the accumulation of the two HMs. Moreover, the molecular mechanisms activated in the roots were investigated paying attention to transcription factors (bHLH family), heavy metal transporters and genes involved in the biosynthesis of metal chelators (nicotianamine and mugineic acid). Cd and Pb are accumulated following different molecular strategies by durum wheat plants, even if the two metals interact with each other influencing their respective uptake and translocation. Finally, we demonstrated that some genes (bHLH 29, YSL2, ZIF1, ZIFL1, ZIFL2, NAS2 and NAAT) were induced in the durum wheat roots only in response to Cd.

scholarly journals Molecular Responses to Cadmium Exposure in Two Contrasting Durum Wheat Genotypes

2021 ◽  
Vol 22 (14) ◽  
pp. 7343
Author(s):  
Erika Sabella ◽  
Andrea Luvisi ◽  
Alessandra Genga ◽  
Luigi De Bellis ◽  
Alessio Aprile
Keyword(s):  
Heavy Metal ◽  
Transcription Factors ◽  
Durum Wheat ◽  
Molecular Mechanisms ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Ros Scavenging ◽  
Metal Transporters ◽  
New Findings ◽  
Cd Translocation

Cadmium is a heavy metal that can be easily accumulated in durum wheat kernels and enter the human food chain. Two near-isogenic lines (NILs) with contrasting cadmium accumulation in grains, High-Cd or Low-Cd (H-Cd NIL and L-Cd NIL, respectively), were used to understand the Cd accumulation and transport mechanisms in durum wheat roots. Plants were cultivated in hydroponic solution, and cadmium concentrations in roots, shoots and grains were quantified. To evaluate the molecular mechanism activated in the two NILs, the transcriptomes of roots were analyzed. The observed response is complex and involves many genes and molecular mechanisms. We found that the gene sequences of two basic helix–loop–helix (bHLH) transcription factors (bHLH29 and bHLH38) differ between the two genotypes. In addition, the transporter Heavy Metal Tolerance 1 (HMT-1) is expressed only in the low-Cd genotype and many peroxidase genes are up-regulated only in the L-Cd NIL, suggesting ROS scavenging and root lignification as active responses to cadmium presence. Finally, we hypothesize that some aquaporins could enhance the Cd translocation from roots to shoots. The response to cadmium in durum wheat is therefore extremely complex and involves transcription factors, chelators, heavy metal transporters, peroxidases and aquaporins. All these new findings could help to elucidate the cadmium tolerance in wheat and address future breeding programs.

scholarly journals The occurrence of fungi on roots and stem bases of common wheat (Triticum aestivum ssp. vulgare L.) and durum wheat (Triticum durum Desf.) grown under two levels of chemical protection

2012 ◽  
Vol 64 (3) ◽  
pp. 93-102
Author(s):  
Irena Kiecana ◽  
Leszek Rachoń ◽  
Elżbieta Mielniczuk ◽  
Grzegorz Szumiło
Keyword(s):  
Durum Wheat ◽  
Common Wheat ◽  
Triticum Durum ◽  
Experimental Treatment ◽  
Bipolaris Sorokiniana ◽  
Wheat Roots ◽  
Chemical Protection ◽  
The Mean ◽  
Triticum Durum Desf ◽  
The University

Investigations were carried out in 2007-2009 on the plots of the Felin Experimental Station belonging to the University of Life Science in Lublin. The studies comprised two cultivation lines of durum wheat (<i>Triticum durum</i> L.): STH 716 and STH 717, as well as the 'Tonacja' cultivar of common wheat (<i>T. aestivum</i> ssp. <i>vulgare</i> L.). Two levels of chemical protection were applied in the cultivation: minimal and complex protection. Infection of wheat roots and stem bases was recorded in each growing season at hard dough stage (87 in Tottman's scale, 1987). After three years of study, the mean disease indexes for the analyzed wheat genotypes in the experimental treatment with minimal protection were 31.13, 30.43 and 38.83 for, respectively, the 'Tonacja' cultivar and the cultivation lines of <i>T. durum</i> STH 716 and STH 717. In the experimental combination with complex protection, after three years of study the disease indexes ranged from 25.26 (<i>T. durum</i> STH 716) to 30.83 (<i>T. durum</i> STH 717). The results of mycological analysis of diseased plants showed that <i>Fusarium</i> spp., especially <i>F. culmorum</i>, <i>F. avenaceum</i> as well as <i>Bipolaris sorokiniana</i> and <i>Rhizoctonia solani</i>, caused root rot and necrosis of wheat stem bases. The analyzed chemical protection levels did not significantly influence grain yield of the investigated genotypes of <i>T. aestivum</i> and <i>T. durum</i>.

scholarly journals Molecular Mechanisms of Proline-Mediated Tolerance to Toxic Heavy Metals in Transgenic Microalgae

2002 ◽  
Vol 14 (11) ◽  
pp. 2837-2847 ◽  
Author(s):  
Surasak Siripornadulsil ◽  
Samuel Traina ◽  
Desh Pal S. Verma ◽  
Richard T. Sayre
Keyword(s):  
Heavy Metals ◽  
Molecular Mechanisms ◽  
Toxic Heavy Metals

Novel Bioremediation Methods in Waste Management

2020 ◽  
pp. 1627-1643 ◽  
Author(s):  
Charu Gupta ◽  
Dhan Prakash
Keyword(s):  
Heavy Metals ◽  
Genetically Modified Organisms ◽  
Treatment Method ◽  
Toxic Heavy Metals ◽  
Novel Methods ◽  
Contaminated Waste ◽  
Oily Waste ◽  
Electro Coagulation ◽  
Cadmium And Lead ◽  
New Generation

Bioremediation technologies are one of the novel methods in the field of waste and environment management and are presently gaining immense credibility for being eco-compatible. Bioremediation using microbes has been well accepted as an environment friendly and economical treatment method for disposal of hazardous petroleum hydrocarbon contaminated waste (oily waste). Besides this, earthworms can be used to extract toxic heavy metals, including cadmium and lead, from solid waste from domestic refuse collection and waste from vegetable and flower markets. Other novel methods used recently for treatment of wastes are plasma incineration or plasma assisted gasification and pyrolysis technology. The technologies applied for conditioning include ultrasonic degradation, chemical degradation, enzyme addition, electro-coagulation and biological cell destruction. Genetic engineering is another method for improving bioremediation of heavy metals and organic pollutants. Transgenic plants and associated bacteria constitute a new generation of genetically modified organisms for bioremediation.

Novel Bioremediation Methods in Waste Management

2016 ◽  
pp. 141-157 ◽  
Author(s):  
Charu Gupta ◽  
Dhan Prakash
Keyword(s):  
Heavy Metals ◽  
Genetically Modified Organisms ◽  
Treatment Method ◽  
Toxic Heavy Metals ◽  
Novel Methods ◽  
Contaminated Waste ◽  
Oily Waste ◽  
Electro Coagulation ◽  
Cadmium And Lead ◽  
New Generation

Bioremediation technologies are one of the novel methods in the field of waste and environment management and are presently gaining immense credibility for being eco-compatible. Bioremediation using microbes has been well accepted as an environment friendly and economical treatment method for disposal of hazardous petroleum hydrocarbon contaminated waste (oily waste). Besides this, earthworms can be used to extract toxic heavy metals, including cadmium and lead, from solid waste from domestic refuse collection and waste from vegetable and flower markets. Other novel methods used recently for treatment of wastes are plasma incineration or plasma assisted gasification and pyrolysis technology. The technologies applied for conditioning include ultrasonic degradation, chemical degradation, enzyme addition, electro-coagulation and biological cell destruction. Genetic engineering is another method for improving bioremediation of heavy metals and organic pollutants. Transgenic plants and associated bacteria constitute a new generation of genetically modified organisms for bioremediation.

scholarly journals Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture

2020 ◽  
Vol 71 (3) ◽  
pp. 197-204
Author(s):  
Dragana Javorac ◽  
Aleksandra Buha Đorđević ◽  
Milena Anđelković ◽  
Simona Tatović ◽  
Katarina Baralić ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Molecular Mechanisms ◽  
Real Life ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Special Importance ◽  
Essential Metal ◽  
Cadmium And Lead ◽  
The Brain ◽  
Insight Into

AbstractMost Pb and Cd neurotoxicity studies investigate exposure to either of the toxic metals alone, while data on co-exposure are scarce. The aim of our study was to fill that gap by investigating acute combined effects of Pb and Cd on redox and essential metal status in the brain of Wistar rats. Animals were randomised in four groups of six to eight rats, which received 15 or 30 mg/kg of Cd, 150 mg/kg of Pb, or 150 mg/kg of Pb + 15 mg/kg of Cd by gavage. The fifth, control, group received distilled water only. Co-treatment with Pb and Cd induced significant increase in malondialdehyde (MDA) and thiobarbituric acid-reactive substances (TBARS) compared to control and groups receiving either metal alone. This is of special importance, as MDA presence in the brain has been implicated in many neurodegenerative disorders. The groups did not significantly differ in Zn, Cu, Mn, and Fe brain levels. Our findings highlight the importance of metal mixture studies. Neurotoxicity assessments of single chemicals do not provide a real insight into exposure to mixtures in real life. Further research should look into interactions between these metals to reveal complex molecular mechanisms of their neurotoxicity.

scholarly journals Bioaccumulation Levels and Potential Health Risks of Mercury, Cadmium, and Lead in Albacore (Thunnus alalunga, Bonnaterre, 1788) from The Aegean Sea, Greece

2019 ◽  
Vol 16 (5) ◽  
pp. 821 ◽  
Author(s):  
Nikolaos Stamatis ◽  
Nikolaos Kamidis ◽  
Pelagia Pigada ◽  
Despoina Stergiou ◽  
Argyris Kallianiotis
Keyword(s):  
Health Risks ◽  
Graphite Furnace ◽  
Aegean Sea ◽  
Absorption Spectrometry ◽  
Consumer Health ◽  
The European Union ◽  
Toxic Heavy Metals ◽  
Potential Health ◽  
Gf Aas ◽  
Cadmium And Lead

Aegean Sea albacore (T. alalunga), fresh or processed, is marketed locally in Greece or exported, mainly to Japan, Italy, Spain, and France. To provide information for consumers and biomonitoring programs and assess the potential human health risks, concentrations of mercury (Hg), cadmium (Cd) and lead (Pb) were determined in albacore edible muscle samples from two fishing grounds of the Aegean Sea, Greece via graphite furnace atomic absorption spectrometry (GF-AAS). Of the 82 individuals, 28 contained Cd and three contained Pb above the permissible limits set by the European Union (0.1 mg kg−1 wet wt and 0.3 mg kg−1 wet wt, respectively). None of the samples contained mercury above the limit (1.0 mg kg−1 wet wt). Potential health risks to human via dietary intake of albacore were estimated by the total target hazard quotients (TTHQs), which indicated that the consumers could acquire health problems due to consumption of Aegean Sea albacore. Thus consequently, concentrations of toxic heavy metals in albacore, especially mercury, must be monitored regularly and comprehensively with respect to consumer health.

Combined Effect of Blood Cadmium and Lead Levels on Coronary Heart Disease Prediction Risk in Korean Men

Angiology ◽  
2015 ◽  
Vol 67 (6) ◽  
pp. 582-586 ◽  
Author(s):  
Hye-Min Cho ◽  
Doo-Yeoun Cho ◽  
Min-Young Kim ◽  
Sung-Won Yang ◽  
Young-Seok Seo ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Combined Effect ◽  
Disease Prediction ◽  
Blood Cadmium ◽  
Lead Levels ◽  
Prediction Risk ◽  
Cadmium And Lead

Evaluation of drought resistance-related traits in durum wheat somaclonal lines selected in vitro

2004 ◽  
Vol 44 (1) ◽  
pp. 27 ◽  
Author(s):  
M. Bajji ◽  
P. Bertin ◽  
S. Lutts ◽  
J-M. Kinet
Keyword(s):  
Durum Wheat ◽  
Somaclonal Variation ◽  
Drought Resistance ◽  
In Vitro Selection ◽  
Culture Media ◽  
Field Studies ◽  
Resistance Mechanisms ◽  
Wide Range ◽  
Triticum Durum Desf

Somaclonal variation associated with in vitro selection has been used as a source of variability to improve drought resistance of 3 durum wheat (Triticum durum Desf.) cultivars (Selbera, Sebou, and Kyperounda). In a previous study, R0 plants with improved drought resistance-related characters were regenerated after selection on culture media containing polyethylene glycol (PEG). This improvement was transmitted to the R1 progeny. The present study analysed the behaviour of the selected tissue culture-derived lines in subsequent R2, R3 and R4�generations. Differences in electrolyte leakage, chlorophyll fluorescence (Fv/Fm), stomatal conductance and days to heading were found between the parental cultivars and most of their in vitro-derived lines. The changes may differ from one cultivar to another. Many promising somaclonal lines still presented improvement for at least 3 of the 4�parameters measured comparatively to initial cultivars. Somaclonal variation thus appears to induce a wide range of modifications among individual components of drought-resistance mechanisms. These improved traits could be valuable if shown to be inherited and to give enhanced agronomic performances in future field studies.

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