scholarly journals Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells

2020 ◽  
Vol 21 (5) ◽  
pp. 1618 ◽  
Author(s):  
Mingxi Jia ◽  
Wenjing Zhang ◽  
Taojin He ◽  
Meng Shu ◽  
Jing Deng ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Food Packaging ◽  
Antibacterial Properties ◽  
Human Colon ◽  
Ag Nps ◽  
Damage Potential ◽  
Human Colon Cancer ◽  
Colon Cells ◽  
Antibacterial Textiles ◽  
The Many

Nano Ag has excellent antibacterial properties and is widely used in various antibacterial materials, such as antibacterial medicine and medical devices, food packaging materials and antibacterial textiles. Despite the many benefits of nano-Ag, more and more research indicates that it may have potential biotoxic effects. Studies have shown that people who ingest nanoparticles by mouth have the highest uptake in the intestinal tract, and that the colon area is the most vulnerable to damage and causes the disease. In this study, we examined the toxic effects of different concentrations of Ag-NPs on normal human colon cells (NCM460) and human colon cancer cells (HCT116). As the concentration of nanoparticles increased, the activity of the two colon cells decreased and intracellular reactive oxygen species (ROS) increased. RT-qPCR and Western-blot analyses showed that Ag NPs can promote the increase in P38 protein phosphorylation levels in two colon cells and promote the expression of P53 and Bax. The analysis also showed that Ag NPs can promote the down-regulation of Bcl-2, leading to an increased Bax/Bcl-2 ratio and activation of P21, further accelerating cell death. This study showed that a low concentration of nano Ag has no obvious toxic effect on colon cells, while nano Ag with concentrations higher than 15 μg/mL will cause oxidative damage to colon cells.

scholarly journals Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ragaa A. Hamouda ◽  
Mervat H. Hussein ◽  
Rasha A. Abo-elmagd ◽  
Salwa S. Bawazir
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Fourier Transforms ◽  
Human Colon ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Ag Nps ◽  
Human Colon Cancer ◽  
Visible Absorption ◽  
Ft Ir

Abstract Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30–17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.

scholarly journals Fabrication, Characterization, and Antibacterial Properties of Electrospun Membrane Composed of Gum Karaya, Polyvinyl Alcohol, and Silver Nanoparticles

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Vinod Vellora Thekkae Padil ◽  
Nhung H. A. Nguyen ◽  
Alena Ševců ◽  
Miroslav Černík
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Polyvinyl Alcohol ◽  
Food Packaging ◽  
Chemical Reduction ◽  
Antibacterial Properties ◽  
Hydroxyl Groups ◽  
Ag Nps ◽  
Gum Karaya ◽  
Nanofiber Membranes

Gum karaya (GK), a natural hydrocolloid, was mixed with polyvinyl alcohol (PVA) at different weight ratios and electrospun to produce PVA/GK nanofibers. An 80 : 20 PVA/GK ratio produced the most suitable nanofiber for further testing. Silver nanoparticles (Ag-NPs) were synthesised through chemical reduction of AgNO3(at different concentrations) in the PVA/GK solution, the GK hydroxyl groups being oxidised to carbonyl groups, and Ag+cations reduced to metallic Ag-NPs. These PVA/GK/Ag solutions were then electrospun to produce nanofiber membranes containing Ag-NPs (Ag-MEMs). Membrane morphology and other characteristics were analysed using scanning electron microscopy coupled with energy dispersive X-ray analysis, transmission electron microscopy, and UV-Vis and ATR-FTIR spectroscopy. The antibacterial activity of the Ag-NP solution and Ag-MEM was then investigated against Gram-negativeEscherichia coliandPseudomonas aeruginosaand Gram-positiveStaphylococcus aureus. Our results show that electrospun nanofiber membranes based on natural hydrocolloid, synthetic polymer, and Ag-NPs have many potential uses in medical applications, food packaging, and water treatment.

Cocoplum (Chrysobalanus icaco L.) anthocyanins exert anti-inflammatory activity in human colon cancer and non-malignant colon cells

2017 ◽  
Vol 8 (1) ◽  
pp. 307-314 ◽  
Author(s):  
Vinicius P. Venancio ◽  
Paula A. Cipriano ◽  
Hyemee Kim ◽  
Lusânia M. G. Antunes ◽  
Stephen T. Talcott ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Human Colon ◽  
Inflammatory Activity ◽  
Human Colon Cancer ◽  
Colon Cells ◽  
Anti Inflammatory

Cocoplum anthocyanins reduced cell proliferation in cancer cells and decreased inflammation in both non-malignant and cancer cells.

scholarly journals CHK Methylation Is Elevated in Colon Cancer Cells and Contributes to the Oncogenic Properties

2021 ◽  
Vol 9 ◽  
Author(s):  
Shudong Zhu ◽  
Yan Zhu ◽  
Qiuwen Wang ◽  
Yi Zhang ◽  
Xialing Guo
Keyword(s):  
Dna Methylation ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Dna Methyltransferases ◽  
Colon Cancer Cells ◽  
Normal Colon ◽  
Human Colon Cancer ◽  
Colon Cells ◽  
Matrigel Invasion

Src is an important oncogene that plays key roles in multiple signal transduction pathways. Csk-homologous kinase (CHK) is a kinase whose molecular roles are largely uncharacterized. We previously reported expression of CHK in normal human colon cells, and decreased levels of CHK protein in colon cancer cells leads to the activation of Src (Zhu et al., 2008). However, how CHK protein expression is downregulated in colon cancer cells has been unknown. We report herein that CHK mRNA was decreased in colon cancer cells as compared to normal colon cells, and similarly in human tissues of normal colon and colon cancer. Increased levels of DNA methylation at promotor CpG islands of CHK gene were observed in colon cancer cells and human colon cancer tissues as compared to their normal healthy counterparts. Increased levels of DNA methyltransferases (DNMTs) were also observed in colon cancer cells and tissues. DNA methylation and decreased expression of CHK mRNA were inhibited by DNMT inhibitor 5-Aza-CdR. Cell proliferation, colony growth, wound healing, and Matrigel invasion were all decreased in the presence of 5-Aza-CdR. These results suggest that increased levels of DNA methylation, possibly induced by enhanced levels of DNMT, leads to decreased expression of CHK mRNA and CHK protein, promoting increased oncogenic properties in colon cancer cells.

scholarly journals Tectorigenin inhibits Caco-2 human colon cells via NF-κB pathway suppression

2015 ◽  
Vol 10 (4) ◽  
pp. 948
Author(s):  
Xiao-Yu Dai ◽  
Yong-Ming Yu ◽  
Zheng-Fang Kong ◽  
Yi-Sheng Cao ◽  
Dan-Dan Huang ◽  
...  
Keyword(s):  
Human Colon ◽  
Pathological Process ◽  
Human Colon Cancer ◽  
Colon Cells ◽  
Reverse Transcription Pcr ◽  
Contagious Diseases ◽  
Tnf Α ◽  
Cytokine Tumor Necrosis Factor ◽  
Factor Α ◽  
Necrosis Factor

<p class="Abstract">Effective immunomodulator and pro-inflammatory cytokine, Tumor necrosis factor-α (TNF-α) are considered to be responsible for connecting autoimmune pathological process and contagious diseases. TNF-α stimulates the C-X-C motif chemokine 10 (CXCL10) expression that is participated in migration of tumor, metastasis, and invasion. Tectorigenin, an o-methylated isoflavone, is present as a major portion in the Iris tectorum rhizomes. In this study, we investigated the tectorigenin effects as an anticancer drug. The obtained results showed that tectorigenin hinders the invasion of human colon cancer cells (Caco-2). We used reverse transcription PCR, q-PCR and enzyme linked immunosorbent assays to test whether the tectorigenin is involved in the inhibition of TNF-α induced CXCL-10 expression in the Caco-2 cell lines. Further, we tested TNF-α induced NF-κB activity using the tectorigenin. Collective results showed that tectorigenin inhibits the CXCL10 production by using TNF-α via NF-κB inhibition.</p><p> </p>

Cellulose nanocrystals/silver nanoparticles: in-situ preparation and application in PVA films

Holzforschung ◽  
2020 ◽  
Vol 74 (5) ◽  
pp. 523-528 ◽  
Author(s):  
Li Fan ◽  
Hui Zhang ◽  
Mengxi Gao ◽  
Meng Zhang ◽  
Pengtao Liu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cellulose Nanocrystals ◽  
Food Packaging ◽  
Moisture Absorption ◽  
Antibacterial Properties ◽  
Nanocomposite Films ◽  
Hydroxyl Groups ◽  
In Situ Preparation ◽  
The Many

AbstractWith the increasing application of polyvinyl alcohol (PVA) films in the field of food packaging, it is important to improve its mechanical and antibacterial properties. This paper focuses on the preparation of PVA nanocomposite films and how their properties are affected by a silver-loaded nanocellulose solution. Cellulose nanocrystals (CNCs) were used as both the carrier and the dispersant of silver nanoparticles (AgNPs) prepared using glucose as the reducing agent. Ag+ was stabilized by the many hydroxyl groups located in the CNCs, and then the Ag+ was reduced to AgNPs in situ. After addition of silver-loaded nanocellulose, the tensile strength of the CNC-PVA-AgNP films increased from 47 MPa to 73 MPa, and the nanocomposite films displayed reduced moisture absorption and good antibacterial properties.

Induction of Apoptic Features in Human Colon Cancer Cells After Exposure to Specific Vitamin Combinations

2000 ◽  
Vol 6 (S2) ◽  
pp. 892-893
Author(s):  
B. Brown ◽  
K. R. Garvin ◽  
B. G. Hughes ◽  
M. D. Standing ◽  
K. L. O'Neill ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Colon Cells ◽  
Human Cancer Cells ◽  
Induction Of Apoptosis ◽  
Programed Cell Death ◽  
Ht 29

Proper nutrition and vitamin consumption have long been associated with lower risks for many human cancers. Data from recent studies have suggested that exposure to antioxidant vitamins and vitamin combinations can induce genetic programed cell death, apoptosis, in human cancer cells. ‘“3 In this study, we report on the induction of apoptosis in HT-29 human colon adenocarcinoma cells after exposure to low doses of 13- c/s-retinoic acid (RA) and vitamin E succinate (VES). Induction of apoptosis was seen only in cancerous colon cells and not in normal human colon cells when exposed to RA and VES in combination, but not when exposed to either vitamin alone.Cultures of HT-29 cells were seeded in 6 well plates at 10,000 cells/well and grown at 37°C, 5% C02 in RPMI 1640 medium supplemented with 10% fetal bovine serum.

scholarly journals Cytotoxic Effect of Cholesterol Metabolites on Human Colonic Tumor (Caco-2) and Non-Tumor (CCD-18Co) Cells and Their Potential Implication in Colorectal Carcinogenesis

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 56
Author(s):  
Mussa Makran ◽  
Gabriel López-García ◽  
Guadalupe Garcia-Llatas ◽  
Reyes Barberá ◽  
Amparo Alegría ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Viability ◽  
Cytotoxic Effect ◽  
Cellular Proliferation ◽  
Human Colon ◽  
Colorectal Carcinogenesis ◽  
Human Colon Cancer ◽  
Colon Cells ◽  
Potential Implication ◽  
The One

Unabsorbed cholesterol, along with that of bile secretions and flaked colon cells, can be metabolized by colonic microbiota. The generated metabolites have been proposed as promoters of colorectal cancer (CRC). In this study, the cytotoxicity (MTT assay) of the main commercially available cholesterol-derived metabolites (coprostanol, cholestanol, coprostanone, and cholestenone) on human colon cancer (Caco-2) and non-tumor (CCD-18Co) cells was evaluated at different physiologically relevant concentrations (9.4–300 µM) and incubation times (24–72 h). In general, the metabolites that most reduced cell viability were coprostanone (54–85% in Caco-2 and 20–81% in CCD- 18Co) and cholestenone (17–91% in Caco-2 and 14–81% in CCD-18Co). These two metabolites are the most hydrophobic, thus reflecting a possible relationship between hydrophobicity and cytotoxicity. Moreover, cholestenone (IC50 at 72 h: 5 ± 1 µg/mL) should be considered cytotoxic on CCD- 18Co cells (non-tumor cells) since it shows an IC50 close to the one considered toxic (<4 µg/mL). Furthermore, CCD-18Co cells are more vulnerable to the cytotoxic effect of cholesterol metabolites. Possible compensatory responses, attenuating the reduction in cell viability caused by cholesterol metabolites, were observed, however these reactions could favor inflammation, resistance to apoptosis, and cellular proliferation, likely contributing to the development of CRC. In conclusion, cholesterol metabolites, mainly the most hydrophobic, could act as promoters of CRC through their cytotoxic activity.

Bile acid hydrophobicity is correlated with induction of apoptosis and/or growth arrest in HCT116 cells

2001 ◽  
Vol 356 (2) ◽  
pp. 481-486 ◽  
Author(s):  
Ashley A. POWELL ◽  
Janna M. LaRUE ◽  
A. K. BATTA ◽  
Jesse D. MARTINEZ
Keyword(s):  
Colon Cancer ◽  
Biological Activity ◽  
Bile Acid ◽  
Bile Acids ◽  
Growth Arrest ◽  
Cancer Cell Line ◽  
Human Colon ◽  
Colon Cancer Cell Line ◽  
Human Colon Cancer ◽  
Colon Cells

Faecal bile acids have long been associated with colon cancer; highly hydrophobic bile acids, which induce apoptosis, have been implicated in the promotion of colon tumours. The moderately hydrophobic chemopreventive agent ursodeoxycholic acid (UDCA) does not induce apoptosis; rather, it causes colon-derived tumour cells to arrest their growth. To investigate the relationship between bile acid hydrophobicity and biological activity we examined 26 bile acids for their capacity to induce apoptosis or alter cell growth. We found that the rapidity with which, and the degree to which, bile acids could induce apoptosis or growth arrest was correlated with their relative hydrophobicities. Of the bile acids tested, only deoxycholic acid (DCA) and chenodeoxycholic acid, the most hydrophobic bile acids tested, could induce apoptosis in less than 12h in the human colon cancer cell line HCT116. The moderately hydrophobic bile acids hyoDCA, lagoDCA, norDCA, homoUDCA and isoUDCA induced growth arrest at 12h but longer incubations resulted in apoptosis. Conjugation of glycine or taurine to the bile acids decreased relative hydrophobicity and eliminated biological activity in our assays. In addition, we tested a subset of these bile acids for their ability to translocate across cell membranes. When 14C-labelled and 3H-labelled DCA, UDCA and lagoDCA were added to cell cultures, we found only minimal uptake by colon cells, whereas hepatocytes had considerably higher absorption. These experiments suggest that hydrophobicity is an important determinant of the biological activity exhibited by bile acids but that under our conditions these activities are not correlated with cellular uptake.

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