scholarly journals Assessing Plasmin Generation in Health and Disease

2021 ◽  
Vol 22 (5) ◽  
pp. 2758
Author(s):  
Adam Miszta ◽  
Dana Huskens ◽  
Demy Donkervoort ◽  
Molly J. M. Roberts ◽  
Alisa S. Wolberg ◽  
...  
Keyword(s):  
Research Method ◽  
Bleeding Disorders ◽  
Important Process ◽  
Disease Pathogenesis ◽  
Antifibrinolytic Therapy ◽  
Diet Induced Obesity ◽  
Health And Disease ◽  
Kinetics Of ◽  
Human And Mouse

Fibrinolysis is an important process in hemostasis responsible for dissolving the clot during wound healing. Plasmin is a central enzyme in this process via its capacity to cleave fibrin. The kinetics of plasmin generation (PG) and inhibition during fibrinolysis have been poorly understood until the recent development of assays to quantify these metrics. The assessment of plasmin kinetics allows for the identification of fibrinolytic dysfunction and better understanding of the relationships between abnormal fibrin dissolution and disease pathogenesis. Additionally, direct measurement of the inhibition of PG by antifibrinolytic medications, such as tranexamic acid, can be a useful tool to assess the risks and effectiveness of antifibrinolytic therapy in hemorrhagic diseases. This review provides an overview of available PG assays to directly measure the kinetics of plasmin formation and inhibition in human and mouse plasmas and focuses on their applications in defining the role of plasmin in diseases, including angioedema, hemophilia, rare bleeding disorders, COVID-19, or diet-induced obesity. Moreover, this review introduces the PG assay as a promising clinical and research method to monitor antifibrinolytic medications and screen for genetic or acquired fibrinolytic disorders.

The Salivary miRNome: A Promising Biomarker of Disease

MicroRNA ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Sara Tomei ◽  
Harshitha Shobha Manjunath ◽  
Selvasankar Murugesan ◽  
Souhaila Al Khodor
Keyword(s):  
Current Knowledge ◽  
Transcriptional Level ◽  
Biological Processes ◽  
Circulating Mirnas ◽  
Disease Pathogenesis ◽  
Technical Aspects ◽  
Recent Developments ◽  
Health And Disease ◽  
Non Coding Rnas

: MicroRNAs (miRNAs) are non-coding RNAs ranging from 18-24 nucleotides also known to regulate the human genome mainly at the post-transcriptional level. MiRNAs were shown to play an important role in most biological processes such as apoptosis and in the pathogenesis of many diseases such as cardiovascular diseases and cancer. Recent developments of advanced molecular high-throughput technologies have enhanced our knowledge of miRNAs. MiRNAs can now be discovered, interrogated, and quantified in various body fluids, and hence can serve as diagnostic and therapeutic markers for many diseases. While most studies use blood as a sample source to measure circulating miRNAs as possible biomarkers for disease pathogenesis, fewer studies have assessed the role of salivary miRNAs in health and disease. This review aims at providing an overview of the current knowledge of the salivary miRNome, addressing the technical aspects of saliva sampling and highlighting the applicability of miRNA screening to clinical practice.

scholarly journals Neuropeptide 26RFa (QRFP) is a key regulator of glucose homeostasis and its activity is markedly altered in obese/hyperglycemic mice

2019 ◽  
Vol 317 (1) ◽  
pp. E147-E157 ◽  
Author(s):  
Gaëtan Prévost ◽  
Arnaud Arabo ◽  
Marie-Anne Le Solliec ◽  
Justine Bons ◽  
Marie Picot ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Glucose Homeostasis ◽  
High Fat Diet ◽  
Release Kinetics ◽  
Oral Glucose ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Glucose Challenge ◽  
Kinetics Of

Recent studies have shown that the hypothalamic neuropeptide 26RFa regulates glucose homeostasis by acting as an incretin and increasing insulin sensitivity. In this study, we further characterized the role of the 26RFa/GPR103 peptidergic system in the global regulation of glucose homeostasis using a 26RFa receptor antagonist and also assessed whether a dysfunction of the 26RFa/GPR103 system occurs in obese hyperglycemic mice. First, we demonstrate that administration of the GPR103 antagonist reduces the global glucose-induced incretin effect and insulin sensitivity whereas, conversely, administration of exogenous 26RFa attenuates glucose-induced hyperglycemia. Using a mouse model of high-fat diet-induced obesity and hyperglycemia, we found a loss of the antihyperglcemic effect and insulinotropic activity of 26RFa, accompanied with a marked reduction of its insulin-sensitive effect. Interestingly, this resistance to 26RFa is associated with a downregulation of the 26RFa receptor in the pancreatic islets, and insulin target tissues. Finally, we observed that the production and release kinetics of 26RFa after an oral glucose challenge is profoundly altered in the high-fat mice. Altogether, the present findings support the view that 26RFa is a key regulator of glucose homeostasis whose activity is markedly altered under obese/hyperglycemic conditions.

scholarly journals Kinetics of Neutrophil Subsets in Acute, Subacute, and Chronic Inflammation

2021 ◽  
Vol 12 ◽  
Author(s):  
Suzanne H. Bongers ◽  
Na Chen ◽  
Erinke van Grinsven ◽  
Selma van Staveren ◽  
Marwan Hassani ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chronic Inflammation ◽  
Peripheral Blood ◽  
Acute Inflammation ◽  
Experimental Endotoxemia ◽  
Inflammatory Conditions ◽  
Treatment Naïve ◽  
Health And Disease ◽  
Kinetics Of

At homeostasis the vast majority of neutrophils in the circulation expresses CD16 and CD62L within a narrow expression range, but this quickly changes in disease. Little is known regarding the changes in kinetics of neutrophils phenotypes in inflammatory conditions. During acute inflammation more heterogeneity was found, characterized by an increase in CD16dim banded neutrophils. These cells were probably released from the bone marrow (left shift). Acute inflammation induced by human experimental endotoxemia (LPS model) was additionally accompanied by an immediate increase in a CD62Llow neutrophil population, which was not as explicit after injury/trauma induced acute inflammation. The situation in sub-acute inflammation was more complex. CD62Llow neutrophils appeared in the peripheral blood several days (>3 days) after trauma with a peak after 10 days. A similar situation was found in the blood of COVID-19 patients returning from the ICU. Sorted CD16low and CD62Llow subsets from trauma and COVID-19 patients displayed the same nuclear characteristics as found after experimental endotoxemia. In diseases associated with chronic inflammation (stable COPD and treatment naive HIV) no increases in CD16low or CD62Llow neutrophils were found in the peripheral blood. All neutrophil subsets were present in the bone marrow during homeostasis. After LPS rechallenge, these subsets failed to appear in the circulation, but continued to be present in the bone marrow, suggesting the absence of recruitment signals. Because the subsets were reported to have different functionalities, these results on the kinetics of neutrophil subsets in a range of inflammatory conditions contribute to our understanding on the role of neutrophils in health and disease.

PERAN IBU DALAM PENINGKATAN SISTEM IMUN ANAK DENGAN INFEKSI SALURAN PERNAFASAN AKUT

2019 ◽  
Vol 11 (2) ◽  
pp. 79-86
Author(s):  
Cindy Ayustin Noya ◽  
Angkit Kinasih ◽  
Venti Agustina ◽  
R.Rr Maria Dyah Kurniasari
Keyword(s):  
Immune System ◽  
Acute Respiratory Infection ◽  
Lower Respiratory Tract ◽  
Research Method ◽  
Respiratory Infections ◽  
Acute Respiratory Infections ◽  
Clean Environment ◽  
History Of ◽  
Qualitative Descriptive

Infeksi saluran pernafasan akut atau yang sering disebut ISPA merupakan infeksi pada saluran pernafasan baik saluran pernafasan atas atau bawah.ISPA juga kebanyakan terjadi pada anak balita karena daya tahan tubuh mereka tidak kuat dalam menghadapi penyakit ISPA. ISPA mengakibatkan kematiansekitar15%-20% per tahun pada usia balita di Negara berkembang. Tujuan penelitian ini adalah untuk mengetahui dan menganalisa peran ibu dalam meningkatkan sistem imun anak dengan ISPA.Metode penelitian yang digunakan dalam penelitian ini adalah kualitatif deskriptif dengan sampel purposive sampling.Populasi dan sampel penelitian ini adalah ibu yang mempunyai anak dengan riwayat dan saat ini menderita penyakit ISPA di Batu Gajah Kota Ambon.Partisipan dalam penelitian ini berjumlah 5 orang. Hasil dari penelitian mendapati 4 kategori yaitu pemberian nutrisi pada anak untuk memenuhi kebutuhan agar sistem imunnya terjaga, kebersihan lingkungan, peran ibu dalam melakukan pencegahan pada anaknya yang mengalami ISPA, dan  peran ibu dalam menjaga dan mempertahankan kesehatan anaknya.   Kata kunci: peran ibu, sistem imun, ispa THE ROLE OF MOTHERS IN INCREASING IMMUNE SYSTEM OF CHILDREN WITH ACUTE RESPIRATORY INFECTION    ABSTRACT Acute respiratory infections or often called ARI is an infection of the upper or lower respiratory tract. ARI occurs mostly in children under the age of five because their endurance is not strong in dealing with ARI. ARI results in deaths of around 15%-20% per year at the age of under-five in developing countries. The purpose of this study was to determine and analyze the role of mothers in improving the immune system of children against ARI. The research method used in this study was qualitative descriptive with a purposive sampling sample. Respondents and samples of this study were five mothers who had children with a history of ARI and currently suffering from the disease in Batu Gajah, Ambon City. The results of the study found 4 categories, namely providing nutrition to children to meet their needs so that their immune systems are maintained, clean environment, mother's role in preventing children with ARI, and mother's role to preserve and maintain the health of their children. The findings indicated that in terms of coping or improving the immune system of a child to avoid ARI, it is necessary to have role the of mothers in providing nutrition so that the immune system is boosted, besides that the mother can prevent and protect her child from various diseases, especially ARI. Keywords: role of mothers, immune system, acute respiratory infections

Perempuan dan Liminalitas dalam Tradisi Perkawinan Adat di Timor Tengah Selatan

2020 ◽  
Vol 6 (1) ◽  
pp. 114
Author(s):  
Marsel Eliaser Liunokas
Keyword(s):  
Qualitative Research ◽  
Research Method ◽  
Literature Study ◽  
Qualitative Research Method ◽  
South Central ◽  
A Value ◽  
Traditional Marriage ◽  
The Village ◽  
As If

Timorese culture is patriarchal in that men are more dominant than women. As if women were not considered in traditional rituals so that an understanding was built that valued women lower than men. However, in contrast to the article to be studied, this would like to see the priority of women’s roles in traditional marriages in Belle village, South Central Timor. The role of women wiil be seen from giving awards to their parents called puah mnasi manu mnasi. This paper aims to look at the meaning of the rituals of puah mnasi maun mnasi and the role and strengths that women have in traditional marriage rituals in the village of Belle, South Central Timor. The method used for this research is a qualitative research method using interview techniques with a number of people in the Belle Villa community and literature study to strengthen this writing. Based on the data obtained this paper shows that the adat rituals of puah mnasi manu mnasi provide a value that can be learned, namely respect for women, togetherness between the two families, and brotherhood that is intertwined due to customary marital affrairs.

Aseuk Hatong Antara Seni Berkomunikasi dan Teknologi: Studi Fenomenologi tentang Budaya Bertani Ladang Masyarakat Tatar Karang Priangan Kabupaten Tasikmalaya

Metahumaniora ◽  
2017 ◽  
Vol 7 (3) ◽  
pp. 332
Author(s):  
Samson CMS
Keyword(s):  
Rainy Season ◽  
Research Method ◽  
Technology Development ◽  
Appropriate Technology ◽  
Musical Aesthetics ◽  
Research Show

ABSTRAKTidak mampunya kita mendalami pengetahuan asli kita sendiri mengakibatkanterjadinya disharmoni teknologi dengan kebutuhan di lapangan, tidak terkecuali TeknologiTepat Guna (TTG) dalam pertanian ladang. Tujuan penelitian ini adalah untuk mengetahuiperanan Aseuk Hatong dalam tradisi pertanian ladang di masyarakat Tatar Karang PrianganKabupaten Tasikmalaya yang religius Islami, tapi masih mempertahankan tradisi tersebut.Metode penelitian ini menggunakan studi fenomenologi Schutz. Hasil penelitian menunjukkanbahwa 1) Seni berkomunikasi: (kakawihan) dalam tradisi Aseuk Hatong adalah upayaharmonisasi antara petani dengan alam yang sedang kemarau melalui senandung ringan yangkontekstual. 2) Teknologi Tani: (Aseuk Hatong) adalah suatu alat untuk mengolah untuk tanahladang pada musim kemarau yang dibalut dengan estetika musikal sederhana khas petaniladang, hasil pengembangan teknologi yang tepat guna, menyenangkan, inovatif, fungsional,terjangkau, murah, dan ramah lingkungan. Dengan demikian, dapat dirumuskan bahwa TradisiAseuk Hatong di Tatar Karang Priangan merupakan media persuasif bertani di kala ngahuma(berladang) tidak dibarengi musim penghujan. Tradisi Aseuk Hatong juga merupakanpengembangan teknologi yang sangat memperhitungkan kearifan lokal yang berlaku.Kata kunci: aseuk hatong, kawih, komunikasi seni, teknologi tani, pertanian ladangABST RACTOur inability to deepen our own original knowledge results in disharmony of technologywith the needs in the field, including the Appropriate Technology (TTG) in agriculture. It istherefore that in any development it is often not harmonious with the needs of society. Theobjective of the research is to know the role of Aseuk Hatong in the agricultural tradition in theTatar Karang Priangan community of Tasikmalaya Regency whicht is religiously Islamic yetstill maintains the tradition. The research method is Schutz phenomenology study. The resultsof research show that (1) the art of communicating (kakawihan) in Aseuk Hatong tradition isa harmonious effort between farmers and the dry nature through mild, contextual humming.(2) Farm technology (Aseuk Hatong)is a tool to cultivate the soil, plowing the fields during thedry season by wrapping with simple typical musical aesthetics of field farmers as the resultsof appropriate technology development, fun, innovative, functional, affordable, cheap, andenvironmentally friendly. As the conclusion, it can be said that Aseuk Hatong Tradition in TatarKarang Priangan as a persuasive media farming during ngahuma (farming) not accompaniedby rainy season is a technology development that takes into account local wisdom occur.Keywords: aseuk hatong, kawih, art communication, farming technology

Preliminary Report on the Research of “Activity Centers” in Taidong, Taiwan

2019 ◽  
Vol 40 (2) ◽  
pp. 121-125
Author(s):  
Petr Janda
Keyword(s):  
Preliminary Report ◽  
Research Method ◽  
Local Communities ◽  
Traditional Beliefs ◽  
Aboriginal Communities ◽  
Religious Significance ◽  
Local Actors ◽  
Symbolic Value ◽  
Suitable Location

This report presents current research on aboriginal activity centers in Taidong County, Taiwan, primarily in the townships of Chishang and Yanping with over 30% of the population being of aboriginal ancestry. Taidong County is the region with the most distinctive aboriginal communities in Taiwan. The research attempts to identify the actors behind the operation of such centers and their significance for aboriginal communities. The research investigates the process of selecting suitable location for the facilities, the specific features of such centers, the potential religious significance of the locations including the role of traditional beliefs in predominantly Christian aboriginal communities, the symbolic value of structures built in the traditional style for construction of ethnicity and financing that enables the construction of the facilities and the organization of the festivities held in them. The principle research method used was interviews with local actors including local representatives, organizers of festivities, as well as members of local communities. The research began in 2017.

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