scholarly journals An Exon Skipping in CRS1 Is Associated with Perturbed Chloroplast Development in Maize

2021 ◽  
Vol 22 (19) ◽  
pp. 10668
Author(s):  
Mao Wang ◽  
Kaiwen Li ◽  
Yang Li ◽  
Lingyu Mi ◽  
Zhubing Hu ◽  
...  
Keyword(s):  
Growth And Development ◽  
Chloroplast Development ◽  
Higher Plants ◽  
Exon Skipping ◽  
Mutant Gene ◽  
Single Mutation ◽  
Plant Growth And Development ◽  
Transmission Electron ◽  
Phenotype Analysis ◽  
Morphological Defects

Chloroplasts of higher plants are semi-autonomous organelles that perform photosynthesis and produce hormones and metabolites. They play crucial roles in plant growth and development. Although many seedling-lethal nuclear genes or regulators required for chloroplast development have been characterized, the understanding of chloroplast development is still limited. Using a genetic screen, we isolated a mutant named ell1, with etiolated leaves and a seedling-lethal phenotype. Analysis by BN-PAGE and transmission electron microscopy revealed drastic morphological defects of chloroplasts in ell1 mutants. Genetic mapping of the mutant gene revealed a single mutation (G-to-A) at the 5′ splice site of intron 5 in CRS1, resulting in an exon skipping in CRS1, indicating that this mutation in CRS1 is responsible for the observed phenotype, which was further confirmed by genetic analysis. The incorrectly spliced CRS1 failed to mediate the splicing of atpF intron. Moreover, the quantitative analysis suggested that ZmCRS1 may participate in chloroplast transcription to regulate the development of chloroplast. Taken together, these findings improve our understanding of the ZmCRS1 protein and shed new light on the regulation of chloroplast development in maize.

Some recent advances in studies of silicon in higher plants

1984 ◽  
Vol 304 (1121) ◽  
pp. 537-549 ◽  
Keyword(s):  
Cell Wall ◽  
Growth And Development ◽  
Time Course ◽  
Higher Plants ◽  
Cereal Grains ◽  
Soluble Form ◽  
Plant Growth And Development ◽  
Wide Range ◽  
Complete Exclusion ◽  
Root Endodermis

In comparison with elements commonly associated with the nutrition of higher plants, silicon has received relatively little attention. Recently developed techniques have, however, demonstrated its occurrence in a wide range of tissues and species. Grasses are heavy accumulators, but considerable variation occurs between and within species. The factors involved in uptake, translocation and deposition in different species are not fully understood. Deposition has been investigated in the roots of a number of species. Active or passive uptake or almost complete exclusion has been observed. While deposits most frequently occur in cell wall layers or in cell lumina of the root endodermis, the major influx remains in a soluble form and is translocated to the shoot. Deposition is heavy in grass and cereal inflorescence bracts. Silica has also been detected in the epicarp hairs of cereal grains, and evidence is presented regarding the time course of its accumulation in these hairs. It is suggested that such deposition cannot be entirely attributed to a passive transpiration mediated mechanism. The significance of these deposits is discussed in relation to plant growth and development, and to wider aspects associated with human health.

scholarly journals Exogenous salicylic acid treatments enhance tolerance to salinity of wheat (Triticum aestivum) plantlets

2010 ◽  
pp. 34-38
Author(s):  
Cornelia Purcǎrea ◽  
Dorina Cachită ◽  
Adriana Petrus ◽  
Liviu Pop ◽  
Adriana Chis
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Growth And Development ◽  
Higher Plants ◽  
Plant Growth And Development ◽  
Physiological Processes ◽  
Negative Effect ◽  
Exogenous Treatment ◽  
Exogenous Salicylic Acid ◽  
Tolerance To Salinity

Salt stress, an abiotic stress, determines modifications of some biochemical indicators, like, antioxidant enzymes, proline (amino acidaccumulate in higher plants under salinity stress) content, and some physiological processes including: plant growth and development. Inthis paper we studied the influence of exogenous treatment of wheat seeds, with 0.1 mM salicylic acid (SA) solution, in the plant response tosalt stress. The treatment was applied by presoaking the seeds in the treatment solution for 12 hours before germination. The results showedthat exogenous 0.1 mM SA solution, administrated to the wheat cariopses significantly ameliorated the negative effect of salt stress in firstweek of germination in laboratory conditions.

scholarly journals Research Progress of PPR Proteins in RNA Editing, Stress Response, Plant Growth and Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Tengfei Qin ◽  
Pei Zhao ◽  
Jialiang Sun ◽  
Yuping Zhao ◽  
Yaxin Zhang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rna Editing ◽  
Growth And Development ◽  
Higher Plants ◽  
Current Knowledge ◽  
Research Progress ◽  
Future Research ◽  
Pentatricopeptide Repeat ◽  
Plant Growth And Development ◽  
Ppr Proteins

RNA editing is a posttranscriptional phenomenon that includes gene processing and modification at specific nucleotide sites. RNA editing mainly occurs in the genomes of mitochondria and chloroplasts in higher plants. In recent years, pentatricopeptide repeat (PPR) proteins, which may act as trans-acting factors of RNA editing have been identified, and the study of PPR proteins has become a research focus in molecular biology. The molecular functions of these proteins and their physiological roles throughout plant growth and development are widely studied. In this minireview, we summarize the current knowledge of the PPR family, hoping to provide some theoretical reference for future research and applications.

scholarly journals Function and regulation of phospholipid signalling in plants

2009 ◽  
Vol 421 (2) ◽  
pp. 145-156 ◽  
Author(s):  
Hong-Wei Xue ◽  
Xu Chen ◽  
Yu Mei
Keyword(s):  
Growth And Development ◽  
Higher Plants ◽  
Plant Growth And Development ◽  
Signalling Molecules ◽  
Cell Responses ◽  
Molecular Approaches ◽  
Multiple Processes ◽  
Hormone Effects ◽  
Messenger Molecule ◽  
Phospholipid Signalling

As an important metabolic pathway, phosphatidylinositol metabolism generates both constitutive and signalling molecules that are crucial for plant growth and development. Recent studies using genetic and molecular approaches reveal the important roles of phospholipid molecules and signalling in multiple processes of higher plants, including root growth, pollen and vascular development, hormone effects and cell responses to environmental stimuli plants. The present review summarizes the current progress in our understanding of the functional mechanism of phospholipid signalling, with an emphasis on the regulation of Ins(1,4,5)P3–Ca2+ oscillation, the second messenger molecule phosphatidic acid and the cytoskeleton.

scholarly journals OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Lan Shen ◽  
Qiang Zhang ◽  
Zhongwei Wang ◽  
Hongling Wen ◽  
Guanglian Hu ◽  
...  
Keyword(s):  
Growth And Development ◽  
Chloroplast Development ◽  
Terminal Region ◽  
Splicing Factors ◽  
Chloroplast Structure ◽  
Plant Growth And Development ◽  
Yeast Two Hybrid ◽  
Phenotypic Analysis ◽  
Additional Information ◽  
Two Hybrid

Abstract Background Chloroplasts play an important role in plant growth and development. The chloroplast genome contains approximately twenty group II introns that are spliced due to proteins encoded by nuclear genes. CAF2 is one of these splicing factors that has been shown to splice group IIB introns in maize and Arabidopsis thaliana. However, the research of the OsCAF2 gene in rice is very little, and the effects of OsCAF2 genes on chloroplasts development are not well characterized. Results In this study, oscaf2 mutants were obtained by editing the OsCAF2 gene in the Nipponbare variety of rice. Phenotypic analysis showed that mutations to OsCAF2 led to albino leaves at the seeding stage that eventually caused plant death, and oscaf2 mutant plants had fewer chloroplasts and damaged chloroplast structure. We speculated that OsCAF2 might participate in the splicing of group IIA and IIB introns, which differs from its orthologs in A. thaliana and maize. Through yeast two-hybrid experiments, we found that the C-terminal region of OsCAF2 interacted with OsCRS2 and formed an OsCAF2-OsCRS2 complex. In addition, the N-terminal region of OsCAF2 interacted with itself to form homodimers. Conclusion Taken together, this study improved our understanding of the OsCAF2 protein, and revealed additional information about the molecular mechanism of OsCAF2 in regulating of chloroplast development in rice.

Identification of calcium oxalate crystals in dried or fixed tobacco leaf

1984 ◽  
Vol 42 ◽  
pp. 288-289
Author(s):  
Vicki L. Baliga ◽  
Mary Ellen Counts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Growth And Development ◽  
Polarized Light ◽  
Calcium Oxalate Crystals ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Calcium is an important element in the growth and development of plants and one form of calcium is calcium oxalate. Calcium oxalate has been found in leaf seed, stem material plant tissue culture, fungi and lichen using one or more of the following methods—polarized light microscopy (PLM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction.Two methods are presented here for qualitatively estimating calcium oxalate in dried or fixed tobacco (Nicotiana) leaf from different stalk positions using PLM. SEM, coupled with energy dispersive x-ray spectrometry (EDS), and powder x-ray diffraction were used to verify that the crystals observed in the dried leaf with PLM were calcium oxalate.

Mechanisms of ethylene biosynthesis and response in plants

2015 ◽  
Vol 58 ◽  
pp. 61-70 ◽  
Author(s):  
Paul B. Larsen
Keyword(s):  
Plant Growth ◽  
Growth And Development ◽  
Ethylene Biosynthesis ◽  
Unsaturated Hydrocarbon ◽  
Flower Senescence ◽  
Detailed Knowledge ◽  
Plant Growth And Development ◽  
Step Process ◽  
Ethylene Response ◽  
Ethylene Signalling

Ethylene is the simplest unsaturated hydrocarbon, yet it has profound effects on plant growth and development, including many agriculturally important phenomena. Analysis of the mechanisms underlying ethylene biosynthesis and signalling have resulted in the elucidation of multistep mechanisms which at first glance appear simple, but in fact represent several levels of control to tightly regulate the level of production and response. Ethylene biosynthesis represents a two-step process that is regulated at both the transcriptional and post-translational levels, thus enabling plants to control the amount of ethylene produced with regard to promotion of responses such as climacteric flower senescence and fruit ripening. Ethylene production subsequently results in activation of the ethylene response, as ethylene accumulation will trigger the ethylene signalling pathway to activate ethylene-dependent transcription for promotion of the response and for resetting the pathway. A more detailed knowledge of the mechanisms underlying biosynthesis and the ethylene response will ultimately enable new approaches to be developed for control of the initiation and progression of ethylene-dependent developmental processes, many of which are of horticultural significance.

Plant hormones, plant growth regulators

2014 ◽  
Vol 155 (26) ◽  
pp. 1011-1018 ◽  
Author(s):  
György Végvári ◽  
Edina Vidéki
Keyword(s):  
Nervous System ◽  
Growth And Development ◽  
Large Scale ◽  
Essential Role ◽  
Higher Plants ◽  
Structure And Function ◽  
Wide Sense ◽  
Large Scale Integration ◽  
Scale Integration ◽  
And Function

Plants seem to be rather defenceless, they are unable to do motion, have no nervous system or immune system unlike animals. Besides this, plants do have hormones, though these substances are produced not in glands. In view of their complexity they lagged behind animals, however, plant organisms show large scale integration in their structure and function. In higher plants, such as in animals, the intercellular communication is fulfilled through chemical messengers. These specific compounds in plants are called phytohormones, or in a wide sense, bioregulators. Even a small quantity of these endogenous organic compounds are able to regulate the operation, growth and development of higher plants, and keep the connection between cells, tissues and synergy beween organs. Since they do not have nervous and immume systems, phytohormones play essential role in plants’ life. Orv. Hetil., 2014, 155(26), 1011–1018.

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